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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Borrelia burgdorferi C t Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... burgdorferi N40 were grown at 33°C in Barbour-Stoenner-Kelly H (BSK-H) complete medium (Sigma-Aldrich, #B8291) with 6% rabbit serum ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were washed three times with PBS-T at 4°C and the specific proteins were visualized with a chemiluminescence HRP substrate system (Millipore, Massachusetts, USA) using LAS 4000 (Fujifilm Life Science ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were then washed in PBS-T and blocked overnight at 4 °C in PBS-T with 1 % bovine serum albumin (BSA, Sigma) and 5 % donkey serum (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... in a mixture of Cyclohexanone:Terpineol (C/T) (398241 and 814759, Sigma Aldrich) at a ratio of 7:3 ...
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bioRxiv - Neuroscience 2023Quote: ... anti-prosurfactant protein C (proSP-C, Millipore Sigma AB3786, RRID: AB_91588) was used at 1:4,000 with microwave antigen retrieval as described above ...
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bioRxiv - Microbiology 2023Quote: ... burgdorferi B31 (PA003) spirochetes were diluted into BSK-II or BSK-II +10mM spermidine (Sigma Aldrich, Catalog #S2626-1G) adjusted to pH 7.8 ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti-Prosurfactant Protein C (pro-SP-C) antibody (1:4000) (Millipore, #AB3786). Antibodies were incubated in 2% powder milk in TBS pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... 30 KDa for T-protein and L-protein and 100 KDa for P-protein) were purchased from Millipore (Billerica, MA, USA). Mut Express II Fast Mutagenesis kit V2 was purchased from Vazyme (Jiangsu ...
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bioRxiv - Cell Biology 2023Quote: ... and protein analysis of tau and β-III-tubulin (Sigma T-8660) by western blot as described above.
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bioRxiv - Plant Biology 2022Quote: ... Membranes were then incubated overnight at 4 °C with 10 ml TBS-T containing either anti-c-myc-peroxidase antibody (1:10,000; Sigma, A5598), or anti-HA primary antibody (1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... burgdorferi at an MOI of 50 for 18h alone or in the presence of 50mM N-acetyl cysteine (NAC; Sigma, A7250). Cells were collected in RIPA buffer (for immunoblotting ...
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bioRxiv - Pathology 2021Quote: ... pro-surfactant protein C (Millipore AB3786, 1:500), p53 (Abcam ab131442 ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... T cells were cultured at 37°C and 5% CO2 in RPMI 1640 (Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-protein kinase C-α (PKCα) (Sigma, P4334); rabbit anti-VSX2 (Chx10 ...
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bioRxiv - Cell Biology 2021Quote: Thioflavin T (ThT, Sigma Aldrich T-3516) was kept as a 10 mM stock solution in distilled water and stored at 4°C in the dark ...
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bioRxiv - Microbiology 2019Quote: ... Protein A/G Plus agarose beads were blocked with bovine t-RNA (1mg/mL; Sigma) for 2h in modified-RIPA buffer ...
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bioRxiv - Genetics 2019Quote: Proteins were precipitated in 23% TCA (Sigma-Aldrich, St. Louis, MO, Product number T-0699) at 4 °C O/N ...
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bioRxiv - Physiology 2020Quote: ... light chain 3B protein (LC3I/II) tubulin (1:1,000; Cat. No. T-5168; Sigma-Aldrich), or mitochondrial porin (1:500 ...
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bioRxiv - Microbiology 2019Quote: B. burgdorferi strain B31 was cultured in BSK-H medium (HiMedia Laboratories Pvt. Ltd.) with 6% rabbit serum (Sigma-Aldrich, St. Louis, MO, USA). All culture medium was filter-sterilized by 0.2 µm filter ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-pro-surfactant protein C (Sigma, Ab3786; 1:2000), rabbit anti-TGFβ2 (Proteintech ...
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bioRxiv - Immunology 2022Quote: ... T cells were cultured at 37°C in RPMI supplemented with 5% heat-inactivated FBS (Sigma-Aldrich), 1% non-essential amino acids (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... T cells were cultured at 37°C in RPMI supplemented with 5% heat-inactivated FBS (Sigma-Aldrich), 1% non-essential amino acids (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... T cells were cultured at 37° C / 5% CO2 in enriched RPMI medium containing HEPES (25mM; Sigma-Aldrich), 2-Mercaptoethanol (50μM ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-surfactant associated protein C (SFTPC, 1:1000, Millipore, AB3786), goat anti-podoplanin (PDPN ...
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bioRxiv - Developmental Biology 2022Quote: ... polyclonal rabbit anti-pro surfactant protein C (1:4000, Millipore, #AB3786), mouse monoclonal acetylated tubulin (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal Prosurfactant Protein C (1:200 pH9, AB3786, Merck Millipore), mouse monoclonal TTF-1 (clone SPT24 ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-pro-surfactant protein C (SFTPC, 1:1000, AB3786, Millipore), goat anti-SOX9 (SOX9 ...
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bioRxiv - Biochemistry 2019Quote: Thioflavin T (Sigma) was dissolved in PBS and filtered through a 0.02 µm filter ...
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bioRxiv - Biophysics 2021Quote: ... Thioflavin-T (Sigma) was added in half-area black 96-well microplates (Corning ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked in 5% milk in TBS-T and incubated overnight at 4°C with the primary antibodies anti-tyrosine hydroxylase (1:1,000; AB152; Merck Millipore) or anti-dopa decarboxylase (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was blocked overnight at 4°C in TBS-T (20 mM Tris, 150 mM NaCl, pH 8.0, 0.1% Tween 20 (Sigma, P9416) plus 5% dry milk (Apex ...
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bioRxiv - Immunology 2022Quote: ... washed 3X with 0.05% Tween20 in PBS (PBS-T) and then incubated overnight at 4 °C with 0.25 µg/well CT (Sigma). OSP and CT ELISAs were then blocked with warm 200 µl/well of R10 media (RPMI + L-Glutamate (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... Blots were incubated with primary antibodies overnight at 4 °C in 2.5 % dry milk and washed with PBS-T (PBS containing 0.1 % v/v Tween 20, Sigma-Aldrich) before incubation with anti-mouse (#7076S ...
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bioRxiv - Immunology 2021Quote: ... the frozen lobes of the right lungs were mechanically homogenized in 300 μl of T-PER protein extraction buffer supplemented with appropriate amount of protease inhibitor cocktail (cOmplete™, Mini Protease Inhibitor Cocktail, Sigma Aldrich; 10 mL T-Per buffer/1 tablet). The suspensions were spun ...
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bioRxiv - Immunology 2020Quote: ... Proteins were precipitated overnight at −20°C with six volumes of pre-chilled (−20°C) acetone (Sigma-Aldrich, USA). The samples were centrifuged at 8,000xg for 10 minutes at 4°C to obtain the protein pellets and supernatants were discarded ...
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bioRxiv - Immunology 2020Quote: ... Proteins were precipitated overnight at −20°C with six volumes of pre-chilled (−20°C) acetone (Sigma-Aldrich, USA). The samples were centrifuged at 8,000xg for 10 minutes at 4°C to obtain the protein pellets and supernatants were discarded ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellet was washed with −20°C cold acetone (Sigma-Aldrich), vortexed ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-amyloid precursor protein (APP, C-terminal, 1:1000, Sigma, #A8717), rat anti-green fluorescent protein (GFP ...
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bioRxiv - Immunology 2024Quote: ... Protein extracts were immunoprecipitated with a PP2A C-specific antibody (1D6; Millipore). An appropriate phosphorylated peptide (amino acid sequence K-R-pT-I-R-R ...
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bioRxiv - Microbiology 2023Quote: ... Enriched proteins were denatured in U/T buffer (6 M urea, 2 M thiourea, 1 mM DTT (Sigma), 10 mM HEPES ...
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bioRxiv - Cancer Biology 2019Quote: ... A preclearing step was made for 2hours at 4°C with 50µl of previously blocked protein-A and protein-G beads (Sigma) for 200µg of chromatin ...
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bioRxiv - Microbiology 2021Quote: ... The cell-free supernatants were stored at –80°C for total protein quantification using the BCA protein assay (Sigma).
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were reduced at 37 °C for 1 hour with 10 mm Tris(2-carboxyethyl)phosphine (TCEP, C-4706 Sigma) in 100 mm NH4HCO3 ...
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bioRxiv - Microbiology 2019Quote: ... mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 °C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO3 ...
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bioRxiv - Microbiology 2021Quote: ... mexicana strain M379 (MNYC/BC/62/M379) promastigotes were grown in T-75 flasks at 26 °C in Schneider’s insect medium (Sigma-Aldrich) supplemented with 0.4 g/L NaHCO3 ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% milk in TBS-T (TBS with 1% Tween-20) and incubated over night at 4°C with the primary anti-FLAG antibody (Sigma F1804-200UG ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with PBS-T containing 2% NGS and antisera against Olig-2 (1:500,Millipore) A2B5 (which stains for OPCs ...
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bioRxiv - Molecular Biology 2023Quote: ... sections were fixed with ice-cold 100 % acetone for 10 min at −20 °C and washed with PBS-T (0.1 % v/v Tween 20, Sigma-Aldrich) before blocking with 5 % BSA in PBS (m/v ...
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bioRxiv - Molecular Biology 2022Quote: ... for one hour and incubated overnight at 4°C in 5% milk/PBS-T with the following antibodies: anti-LIMCH1 (1:400, catalog number HPA063840, Sigma), anti-MBNL1 (1:1,000 ...