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Citations for Millipore Sigma :
501 - 550 of 9718 citations for Beta NGF Mouse 110a.a since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Slides were incubated in a humidified chamber in primary antibody for Amyloid-Beta (6E10) (1:1000; Sigma, Cat#Sig39320) or p-Tau (AT8 ...
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bioRxiv - Pathology 2023Quote: ... Fibers were incubated in an Eppendorf tube with primary antibodies to detect microtubule structure (beta-tubulin; T4026, Sigma-Aldrich) and the population of microtubule tubulin modified by detyrosination (deTyr-tubulin ...
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bioRxiv - Neuroscience 2023Quote: ... dissolved in distilled water with 10% DMSO (99,5%, PanReac AppliChem, 131954.1611) and 30 mg/mL of beta-cyclodextrin (Sigma, H107), based on ref98 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed in laemlli buffer supplemented with beta-mercaptoethanol and HALT protease and phosphatase inhibitor (PPI) cocktail (Sigma). Isolation of nuclei from whole cell lysis adapted a standard centrifugation based protocol [65] ...
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bioRxiv - Cell Biology 2024Quote: ... mixed with 4X Laemmli sample buffer (62.5mM Tris, 10% glycerol, 1%SDS, 0.005% bromophenol blue) containing beta-mercaptoethanol (M6250, Sigma-Aldrich), and incubated for 5 minutes at 95 deg C ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-mouse αSMA (Sigma A2547), goat-anti-human TIE1 (R&D AF619) ...
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bioRxiv - Biophysics 2020Quote: ... Remaining filaments were washed away with assay buffer (25mM Immidizole, 25mM KCl, 4mM MgCl2, 1mM EGTA, 1mM DTT and 10µg/mL Beta Casein (Sigma C6905)) ...
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bioRxiv - Developmental Biology 2020Quote: ... at 56°C for 40min and re-probed with anti– beta-actin antibody for 1h (1:1000, Sigma, ref. A5441).
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bioRxiv - Neuroscience 2020Quote: ... The blots were also probed with primary antibodies against beta-actin (Sigma-Aldrich, St. Louis, MO, diluted at 1:100), which served as a loading control ...
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bioRxiv - Biochemistry 2020Quote: ... the reactions were stopped with EDTA buffer and the samples incubated with 0.1U/mL of chondroitinase ABC (AMS Biotechnology, Abingdon, UK) and keratanase (endo-beta galactosidase, Cat. n.: G6920, Sigma Aldrich) in deglycosylation buffer (50mM sodium acetate ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were then solubilized by mixing 3:1 lysate to Li-Cor 4x loading buffer (928-40004) plus 10% Beta-Mercaptoethanol (Sigma). Samples were denatured by boiling for 5 min followed by cooling on ice for 2 min ...
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bioRxiv - Neuroscience 2022Quote: The MILLIPLEX® MAP human amyloid beta and tau magnetic bead panel 4-plex ELISA kit (Millipore, Burlington, MA, USA) and the Bio-Plex 200 System instrument (Biorad ...
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bioRxiv - Cell Biology 2020Quote: ... the WT and Gaucher MSCs were plated at a density of 2 ×104 cells/cm2 and were cultured in osteoblast differentiation media [MSC media supplemented with 10 mM beta-glycerophosphate (Sigma), 100 µM dexamethasone (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.1 mg/ml of purified PIM1 and PIM1 mutants were incubated at 37 °C with or without 0.08 mg/ml beta-casein (Sigma-Aldrich). Aliquots were taken at specified time intervals and immediately mixed with 6X SDS sample buffer to terminate the reaction ...
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bioRxiv - Cell Biology 2021Quote: Exponentially growing cells were diluted to OD600nm of 0.02 in 25 ml SC-Full containing 1μM beta-estradiol (Sigma-Aldrich E8875) and tripentadecanoin or Ophioglossum whole extract ...
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bioRxiv - Cell Biology 2022Quote: ... or 100 pmol (third and fourth replicate) of the internal standard (22E)-Stigmasta-5,7,22-trien-3-beta-ol (Sigma-Aldrich, R202967) using 100 μl acetic anhydride/chloroform (1:12 v/v ...
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bioRxiv - Immunology 2022Quote: ... The human SIRT1 ligand was dissolved in 10 mM sodium acetate pH 4.0 plus 20 μM NAD+ (catalog number N1636, beta-nicotinamide adenine dinucleotide hydrate, Sigma–Aldrich), which was immobilized onto the Biacore sensor chip according to the manufacturer’s instructions.
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: Visualization of the accessible pool of cholesterol was performed after 30 min treatment with 10 mM methyl-beta-cyclodextrin (MβCB) in 1% lipoprotein-deficient serum (LPDS, Sigma #S5519) or 1% LPDS control ...
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bioRxiv - Cancer Biology 2023Quote: ... rabbit anti-CYR61 (Bio-Techne SAS #NB100-356; diluted 1:1000) or rabbit anti-beta Actin (Sigma #; diluted 1:2000). After three washes with TBST buffer [50 mM Tris/HCl pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... anit-rabbit-IRF3 (CellSignaling, Cat# 4302), rabbit-anti-phospho IRF3 (CellSignaling, Cat# 29047, clone D6O1M) and rabbit-anti-beta-actin (SIGMA), followed by IRDye 800CW or 680RD secondary antibodies (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Aβ and pTau levels in the organoids were quantified with Milliplex human amyloid beta and tau kit (Millipore HNABTMAG-68K) on the Luminex ...
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bioRxiv - Biochemistry 2024Quote: ... cells were sorted into wells containing 1μL of master mix (0.2% n-dodecyl-beta-maltoside (D4641-500MG, Sigma Aldrich, Germany), 100mM tetraethylammonium bicarbonate (17902-500ML ...
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bioRxiv - Developmental Biology 2022Quote: ... Cy3-mouse-anti-mouse aSMA (Sigma C6198), mouse anti-mouse αSMA (Sigma A2547) ...
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bioRxiv - Biophysics 2021Quote: U2OS cell immunostained for microtubules for DNA-PAINT imaging were prepared as previously described6 using anti beta-tubulin antibody (T5293; Sigma-Aldrich).
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bioRxiv - Physiology 2020Quote: ... they were incubated in a humidified atmosphere containing 5% CO2 at 37°C in a chondrogenic medium containing TGF beta-1 (10 ng/ml) (Sigma Aldrich) in DMEM medium supplemented with 10% FBS and 2 mM L-glutamine for up to 2 weeks ...
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bioRxiv - Physiology 2021Quote: Recipient mice were rendered diabetic before islet transplantation with a single intravenous injection of the beta cell toxin streptozotocin (STZ 200 mg/Kg; Sigma, MO) dissolved fresh in 100 mM sodium citrate (pH 4.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The beta subunit of complex V and proteins bound to it were immunoprecipitated using an antibody to the beta subunit of ATP synthase (Millipore, MAB3494) in accordance with the Pierce™ Co-Immunoprecipitation Kit (Cat.No:26149) ...
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bioRxiv - Neuroscience 2022Quote: ... 810-2 was dissolved in a vehicle (VEH) solution of 20% hydroxy propyl beta cyclodextrin (HPβCD; Sigma, St. Louis, MO, USA) in acetate buffer [0.1 M acetic acid (from glacial acetic acid stock ...
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bioRxiv - Physiology 2021Quote: The BHB concentration was quantified using the Beta-Hydroxybutyrate Assay Kit (MAK041) according to the technical bulletin (Sigma-Aldrich, United States). The concentration of BHB was expressed in ng/μg protein.
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bioRxiv - Microbiology 2019Quote: ... Primers (Table S1) used for PCR amplification of the beta-lactams genes (blaTEM, blaSHV, blaCXT-M and blaOXA) were acquired from Sigma Aldrich, USA ...
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bioRxiv - Neuroscience 2021Quote: ... The biochemical assay of GCase activity was carried out in a buffer containing 4-methylumbelliferyl beta-d-glucopyranoside substrate (Cat. M3633, Sigma Aldrich); after 1 hour at 37°C the reaction was stopped with 0.25 M glycine buffer pH 10.4 and the fluorescence emission of the 4-methylumbelliferyl generated (4-MU ...
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bioRxiv - Neuroscience 2023Quote: ... and Aβ 1-42 using the Milliplex® MAP Human Amyloid Beta and Tau Multiplex Assay kit (Millipore Sigma HNABTMAG-68K). All kits were read on a MAGPIX® system (Luminex ...
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bioRxiv - Bioengineering 2023Quote: ... for 5 minutes and suspended in medium (DMEM 85% v/v, Corning; FBS 15% v/v, VWR; 1x Penicillin-Streptomycin, VWR; 50 µM beta-mercaptoethanol, Sigma-Aldrich). 10,000 viable cells were plated per well in white polystyrene 384-well microplates (Greiner Bio-One ...
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bioRxiv - Plant Biology 2023Quote: ... except that 3 µl beta-mercaptoethanol per sample replaced 1,4-dithiothreitol (DTT) and 0.5% polyvinylpyrrolidone (PVP, mw 360 000, Sigma Aldrich, Steinheim, Germany) was added to the extraction buffer ...
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bioRxiv - Microbiology 2023Quote: ... about 50 μg proteins from each sample were reduced with 10 mM tris (2-carboxyethyl) phosphine (TCEP, Adamas-beta) and then alkylated with 40 mM iodoacetamide (IAA, Sigma-Aldrich). Proteins were pre-digested with 0.5 μg trypsin (Hualishi Tech ...
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bioRxiv - Developmental Biology 2023Quote: ... On addition of Osteogenic media (DMEM + Primocin + 10% FBS + 50 µM ascorbic acid + 10 nM dexamethasone + 10 mM beta-glycerophosphate disodium (Sigma, G9422), the cells form mineralized nodules indicating their osteogenic ability as seen in Supplementary Figure 1.
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bioRxiv - Cell Biology 2023Quote: Human EndoC-βH1 and rat INS1E beta cells were cultured as previously described [45] and incubated with lentiviruses expressing an NNAT-targeting shRNA (Sigma-Aldrich) or Silencer Select siRNAs (Ambion) ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-mouse agarose or mouse agarose (both Sigma) were added for 1 hour at 40C prior to 3 washes in Lysis Buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Mouse anti-mouse NeuN (Millipore, MAB377, 1:100), and Rabbit Anti-MBP (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... and Mouse-anti-mouse/rat-NeuN (1;200, Millipore). Secondary antibodies include Goat-anti-Chicken-IgG-488 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... CLK2 (1:1000, sigma, HPA055366-100UL), phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50. Beta-Tubulin (1:5000, Sigma Aldrich, #T7816) and Beta-Actin (1:5000 ...
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bioRxiv - Molecular Biology 2020Quote: ... After 24 hours cells were treated with 100 μM 5,6-dichloro-1-bold beta-D-ribofuranosylbenzimidazole (DRB) (287891, Sigma St. Louis, MO) for 3 hours ...
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bioRxiv - Neuroscience 2020Quote: ... and Zbtb16 (1:250, Santa-Cruz, Mississauga, ON) Beta-actin was used as a reference protein (1:5000, Sigma-Aldrich, Oakville, ON). Blots were scanned by Amersham Imager 600 imaging system (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Microbiology 2020Quote: ... and then incubated overnight at 4 °C with anti-hACE2 antibody (1:1000; R&D AF933) and anti-beta actin (1:2000; CST 4970) in 5% BSA (Sigma-Aldrich A9647) in TBST ...
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bioRxiv - Immunology 2022Quote: ... penicillin-streptomycin (Hyclone, Chicago, IL), 10% heat-inactivated fetal bovine serum (Hyclone, Chicago, IL) and beta-mercaptoethanol (Sigma-Aldrich, St. Louis, MO). Cell lines have been recently authenticated and were periodically tested for mycoplasma species and were confirmed negative.
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bioRxiv - Neuroscience 2023Quote: ... Fixed cells were incubated with staining solution (1mg/mL of X-Gal,5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside; Sigma Aldrich #11680293001)(Lorenzo ...
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bioRxiv - Neuroscience 2023Quote: ... equivalent to about 5 mg/kg/day) was prepared in vehicle made of 0.45% (2-hydroxypropyl)-beta-cyclodextrin (β-CD; both from Sigma-Aldrich, France), and administered in drinking water for 28 days ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA/DNA and alpha/beta tubulin were tested using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 1 µg of PfCoSP was mixed with 500 µl cellulose matrix (1mg/ml ...
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bioRxiv - Genetics 2023Quote: Tissue lysates in RIPA buffer containing protease and phosphatase inhibitors as above were used for ELISA measurements of IL-1 beta (#RAB0275, Sigma Aldrich, GmbH) and IL-6 (#RAB0309 ...