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Citations for Millipore Sigma :
201 - 250 of 6476 citations for B2M Cynomolgus HEK293 Flag His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Flag-beads (ANTI-FLAG® M2 Affinity Agarose Gel; Sigma-Aldrich) were washed twice (20x the beads volume ...
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bioRxiv - Microbiology 2021Quote: ... Flag was stained with the Flag specific antibody (Sigma, cat# F1804) at a dilution of 1:50 ...
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bioRxiv - Molecular Biology 2020Quote: ... and α-FLAG (detecting C-terminal 3x FLAG-tagged constructs, Sigma). Blots were probed using HRP-conjugated Secondary Antibody (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... mouse monoclonal anti-FLAG and anti-FLAG-HRP (clone M2, Sigma), rabbit monoclonal anti-pSer910-LRRK2 (clone UDD1 15(3) ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged proteins were purified using FLAG M2 affinity gel (Sigma) rotated end over end for 3 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies targeting FLAG (1:5,000; Monoclonal ANTI-FLAG M2-peroxidase, Sigma) and HA (1:5,000 ...
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bioRxiv - Neuroscience 2022Quote: ... and FLAG immunoprecipitation was performed using anti-FLAG M2 agarose (Sigma) following protocol described in Lam et al.90 Immunocomplexes were then treated with Lambda phosphatase λ-PP or mock treated (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... and FLAG (mouse-FLAG, 1:1000, Sigma-Aldrich, F1804, RRID: AB_262044) for 48 h at 4 ⁰C ...
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bioRxiv - Systems Biology 2023Quote: ... Anti-FLAG M2 antibody (Anti-Flag M2, F3165-1MG, Millipore-Sigma) was applied to the membrane for 30 min to the protein of interest and with Anti-GFP (Millipore-Sigma ...
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bioRxiv - Immunology 2023Quote: ... Flag-NLRP3 was immunoprecipitated using anti-FLAG M2 agarose beads (Sigma). Interacting proteins of Flag-NLRP3 were analyzed by immunoblotting analysis of the IP samples ...
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bioRxiv - Systems Biology 2023Quote: ... Anti-FLAG M2 antibody (Anti-Flag M2, F3165-1MG, Millipore-Sigma) was applied to the membrane for 30 min to the protein of interest and with Anti-GFP (Millipore-Sigma ...
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bioRxiv - Microbiology 2023Quote: ... 25 μl of Flag antibody (Monoclonal ANTI- FLAG M2, Sigma, #F1804) was added to the supernatant and rotated for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 was detected using anti-FLAG mouse antibody (Sigma) and cMyc using anti-c-Myc rabbit antibody clone 7E18 (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2-agarose (Sigma), following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma; mouse anti-FLAG clone M2 antibodies). For mass spectrometry sample preparation ...
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bioRxiv - Developmental Biology 2020Quote: ... HeLa cell lysates obtained after transfecting cells with flag-hCNOT9 or flag-hCNOT9mut4 or pCDNA3.0 vectors were subjected to immunoprecipitation using ANTI-FLAG M2 Affinity gel (Sigma) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Developmental Biology 2019Quote: ... 6×His-tagged protein were expressed and purified by Ni-NTA His Bind Resin (Millipore 70666-3). ∼0.2 μg of GST-OsBZR1 ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 10% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (Sigma Aldrich) supplemented with 10 % fetal calf serum at 37°C/5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (Sigma Aldrich) supplemented with 10% fetal calf serum at 37°C/5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293 suspension cells were transfected with constructs using polyethylenimine (PEI, Sigma). HEK293 suspension cells were cultured at 37°C and 8% CO2 and 120 rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 and HeLa cells were cultured in DMEM media (Sigma, RNBK7590) supplemented with 10% free fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: HEK293 (CRL-1573-ATCC) were grown in DMEM (Sigma-Aldrich, USA) supplemented with 10 % FBS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells were seeded on poly-L-lysine (Sigma, Cat. #P8920) coated coverslips in 12-well plates ...
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bioRxiv - Biochemistry 2023Quote: HEK293 cells were maintained in DMEM F-12 Ham medium (Sigma) supplemented with 10% fetal bovine serum (GIBCO ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 and HeLa cells were transfected with Polyethyleneimine (PEI, Sigma Aldrich) as previously described (Braun et al. ...
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bioRxiv - Cell Biology 2024Quote: HEK293 (Human Embryonic Kidney, ECACC 96121229 from Sigma-Aldrich Chemie GmbH) were cultured in DMEM (Dulbecco’s modified Eagle’s medium PAN biotech ...
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bioRxiv - Cell Biology 2021Quote: ... and FLAG-mTOR was immunoprecipitated with anti-FLAG beads (EZview™ Red ANTI-FLAG® M2 Affinity Gel, Sigma, F3165). The immunoprecipitates were washed three times with 0.5% CHAPS buffer before proceeding with WB.
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bioRxiv - Molecular Biology 2019Quote: ... and FLAG::CG33172 (pET28a-FLAG-CG33172) were co-expressed in C41 (86) bacteria and purified over Flag-coupled resin (Sigma). Bound proteins were washed 3 times in 500 mM KCl and eluted on Bio-spin disposable chromatography columns (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Flag-Pyrin was immuno-precipitated using anti Flag M2 affinity gel (Sigma). Proteins were separated by SDS/PAGE on precast 4-15% acrylamide gels (Bio-rad ...
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bioRxiv - Molecular Biology 2020Quote: ... and FLAG-tagged ribosomes were purified using M2 FLAG-affinity resin (Sigma), as described previously (Inada et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... and anti-FLAG M2 (Anti-FLAG M2, Sigma-Aldrich, 1:3000 dilution). Image analysis of immunoblots were performed using Image Studio software (Li-Cor).
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bioRxiv - Cell Biology 2019Quote: ... ORF2-Flag were detected using FLAG-M2 antibody (Sigma, cat. number F1804) at 1:1000 dilution.
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bioRxiv - Plant Biology 2021Quote: ... rabbit polyclonal anti-flag epitope (FLAG) (F7425, Sigma, St. Louis, MO, USA) (1:10,000) ...
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bioRxiv - Microbiology 2021Quote: ... a mouse anti-FLAG primary (Sigma Mouse Anti-FLAG M2 monoclonal antibody) was used ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted by competition using the FLAG peptide (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma). Immunoprecipitates were incubated for at least 2 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... FLAG-tagged TSC2 was immunoprecipitated with ANTI-FLAG® agarose beads (Sigma) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-FLAG immunoprecipitation was performed using anti-FLAG agarose beads (M2, Sigma). Immunoprecipitates were washed two times in solubilization buffer containing 0.1% Triton X-100 and eluted in SDS sample buffer and analyzed by western blotting (see below).
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bioRxiv - Cell Biology 2019Quote: ... overnight and immunoprecipitated ARRB1-FLAG were eluted with Flag peptides (F3290, Sigma). For primary antibody incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged ZC3H11A was immunopurified with anti-Flag(M2) magnetic beads (Sigma) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... FLAG-tagged protein signals were detected with anti-FLAG (Sigma-Aldrich, F1804) in combination with anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Flag-ARPC1B were labelled with anti-Flag antibody from mouse (F1804; Sigma) and detected with anti-mouse secondary antibody from goat conjugated with the fluorochrome Alexa Fluor 594 (A11032 ...
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bioRxiv - Microbiology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with FLAG-M2 antibody (F1804 Sigma-Aldrich) conjugated to protein G dynabeads (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... anti-FLAG (F3165, Monoclonal ANTI-FLAG M2, Millipore Sigma, St. Louis, MO), anti-FRB (ALX-215-065-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-FLAG (M2; Sigma), and anti-β-actin (AC-15 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-FLAG M2 (Sigma), anti-Myc antibody (ab32 Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... anti-FLAG M2 (Sigma), anti-Myc antibody (ab32 Abcam) ...