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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for Anti PKC mu since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 50μL of 2mM artificial substrate 4-MU-α-D-galactopyranoside (Sigma) dissolved in 0.06 M phosphate citrate buffer (pH 4.7 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with 6 mU of bleomycin (Sigma-Aldrich, B8416) for 48 h ...
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bioRxiv - Biochemistry 2021Quote: ... cells were treated with 2 mU of Vibrio cholerae (Sigma; #11080725001) or Arthrobacter ureafaciens (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 mM 4-MU N-acetyl-β-D-glucosaminide (Sigma Aldrich) in 200 mM sodium citrate buffer ...
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bioRxiv - Plant Biology 2024Quote: ... through quantification of the fluorescence of 4-MU (4-Methylumbelliferone, Sigma) reaction product ...
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bioRxiv - Microbiology 2020Quote: ... The PKC activator Bryostatin and Sodium Butyrate (NaBu) were purchased from EMD Millipore. Vorinostat (SAHA ...
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bioRxiv - Biochemistry 2020Quote: ... 0.01% Tween 20 100 μM of Mu-HSSKLQ-AMC (Sigma-Aldrich, SCP0224), in 384-well black plates in triplicate or (b ...
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bioRxiv - Biochemistry 2020Quote: ... An equal volume of 200 μM Mu-HSSKLQ-AMC (Sigma-Aldrich, SCP0224) in the same buffer was then added and protease activity was quantified at 25 °C ...
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bioRxiv - Pathology 2021Quote: ... N″-triacetylchitotrioside [4-MU-β-(GlucNAc)3] (Sigma, St. Louis, MO, USA) as the substrate ...
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bioRxiv - Microbiology 2020Quote: ... Prostratin and Bryostatin-1 (PKC activator) were purchased from Sigma-Aldrich (St. Louis, MO), while Panobinostat (HDAC inhibitor) ...
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bioRxiv - Cancer Biology 2021Quote: ... 106 cells were digested with 70 mU Neuraminidase from Clostridium perfringens (Sigma-Aldrich) in sodium acetate buffer at 37 °C overnight ...
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bioRxiv - Immunology 2021Quote: Small interfering RNA (siRNA) to human 3’ UTR PKC-θ was purchased from Sigma Aldrich. YTS-2DL1 cells were transfected with siRNA specific for the indicated genes ...
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bioRxiv - Biophysics 2021Quote: ... and the complex formation was initiated by addition of 25 mU/mL Apyrase (Sigma), 20 mg/mL Nb35 and 10 μM peptide (GenScript) ...
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bioRxiv - Biochemistry 2020Quote: ... and 2.5 mU/μL glucose-6-phosphate dehydrogenase (G6PD from Saccharomyces cerevisiae, Sigma, USA). Some assays were performed in the presence of variable concentrations of Gln (0.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell cultures were incubated with 500 mU/ml ChABC (C3667, Sigma-Aldrich, Taufkirchen, Germany) or 500 U/ml hyaluronidase (HYase ...
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Neuraminidase inhibitors rewire neutrophil function in vivo in murine sepsis and ex vivo in COVID-19bioRxiv - Immunology 2022Quote: ... Leukocytes were incubated in the presence or absence of CpNEU (10 mU, Sigma-Aldrich), CpNEU plus Oseltamivir or CpNEU plus Zanamivir ...
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bioRxiv - Genomics 2022Quote: HC protein fragments were cleaved by thrombin (40 mU/μL) (EMD Millipore, 605157-1KU) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... 37 °C) were treated with snake venom phosphodiesterase (phosphodiesterase I, Sigma, 32 mU/mL). The percent degradation over time (0-360 minutes ...
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bioRxiv - Genetics 2023Quote: ... and 10 mU/µL of phosphodiesterase I (PDEI) from Crotalus adamanteus venom (Sigma Aldrich) made up in a 5X digest buffer composed of 20 mM Tris-HCl (pH 8) ...
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bioRxiv - Neuroscience 2022Quote: ... PKC activator phorbol 12-myristate 13-acetate (TPA; 400 nM, 20 min, Sigma Aldrich; Cat. #SLBX8889) [109] ...
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bioRxiv - Molecular Biology 2019Quote: BJ1hTERT fibroblasts were treated with either a kinase inhibitor that prevents DNA-PKcs autophosphorylation (NU7026, Sigma) or MRE11 endonuclease activity inhibitor (PFM01 ...
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bioRxiv - Bioengineering 2019Quote: ... The supernatant is removed and replaced with 40 uL of 10 mM 4-MU (Sigma) in methanol diluted in 960 uL DPBS ...
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bioRxiv - Microbiology 2021Quote: ... the GAGs were digested with 20 mU/mL Chondroitinase ABC (from Proteus vulgaris, Sigma Aldrich) and incubated for 2 hr at 37°C in lyase buffer (50 mM Tris and 50 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... sections were first treated with 3 mU/ml of Vibrio Cholerae sialidase (Sigma-Aldrich, Germany) prior to incubation with antibodies or VLPs ...
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bioRxiv - Biochemistry 2020Quote: ... 200 µl of ACE (20 mU/mL; from Rabbit lung, A67778, Sigma-Aldrich, MO, USA) was added to 2.17mM hippuryl-L-histidyl-L-leucine (HHL ...
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bioRxiv - Plant Biology 2019Quote: ... and GUS activity was expressed as pmol of 4- methyl-umbelliferone (4-MU, Sigma, USA) per µg protein per min ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were then treated with 10 mU/mL glucose oxidase enzyme (Sigma-Aldrich; Cat#G7141) diluted in 100μL cell culture medium (RPMI-1640 + 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... A standard curve with 800 to 25 pmoles/well of 4-MU (Sigma cat # M1508) was used to extrapolate the molar conversion of MUNANA ...
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bioRxiv - Genetics 2024Quote: ... K562-Bcl2 and K562-PKcs-/- cells were selected with 5 μg/ml puromycin (Sigma-Aldrich, #P8833-100MG), and K562-iCas9 cells were selected with 200 μg/ml G418 (BioVision ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 mM ZnCl2 and 50 mM NaCl) and 5 mU phosphodiesterase II from Sigma (# P9041-10UN) were added and the mixture was incubated at 37°C ...
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bioRxiv - Pathology 2023Quote: ... followed by 2 mL (1.5ml/min) perfusion with HBSS containing collagenase D (0.2 mU/mL, Sigma). Livers were removed and incubated for 30 min at 37°C under gentle agitation with HBSS containing collagenase D (0.2 mU/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... GSL II lectin detection was performed following PNGaseF (250 mU/106 cells, in PBS 1x; Sigma-Aldrich) enzymatic digestion to exclude N-glycan-associated N-acetylglucosamine residues contribution ...
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bioRxiv - Biochemistry 2022Quote: ... Loading of the E2 was stopped by adding apyrase (Sigma-Aldrich A6410, 25 mU/µl final concentration) and incubation for 5 min at 25°C ...
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bioRxiv - Cell Biology 2022Quote: ... As a positive control, iPSDM were treated with glucose oxidase (50 mU/ml GO, 1 h) (Sigma) as previously described17.
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bioRxiv - Biochemistry 2024Quote: ... Incubation with the 4-MU substrate (3 mM in citrate phosphate buffer with 0.2% taurodeoxycholate, Sigma-Aldrich) for 90 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... and incubated for 4 hr at 37 °C with 20 mU/mL chondroitinase ABC (Proteus vulgaris, Sigma Aldrich). The HS was purified over a DEAE column and precipitated with 90% ethanol (Esko ...
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bioRxiv - Immunology 2021Quote: ... the wells were coated directly with the purified active plasmin (10 mU/mL, Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Bioengineering 2024Quote: ... 0.5 mM 4-Methylumbelliferone (4-MU, Selleckchem, in 0.1% v/v DMSO) and 0.1 mM Glycyrrhizin (Sigma-Aldrich) added to the culture media for 3 days (0.1% v/v DMSO and DMEM were used as a controls) ...
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bioRxiv - Neuroscience 2021Quote: ... N2A cells were first incubated the night prior the electrophysiological measurements with the PKA inhibitor KT5720 and the PKC inhibitor GF-109203 (both from Sigma, dissolved in DMSO and both used at a final concentration of 1 μM) ...
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bioRxiv - Molecular Biology 2024Quote: ... Fractions containing DNA-PKcs were pooled and concentrated to a final volume of 50 µL using 100 kDa MWCO spin concentrator (Millipore) centrifuging 5000 rfc at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Fractions containing DNA-PKcs were pooled and concentrated to a final volume of 50 µL using 100 kDa MWCO spin concentrator (Millipore) centrifuging 5000 rfc at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Male C57Bl/6J mice were treated with 5% 4-MU (Alpha Aesar) and 2 mg/ml 4-MUG (Sigma) starting at 8 weeks of age for 20 days ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Male C57Bl/6J mice were treated with 5% 4-MU (Alpha Aesar) and 2 mg/ml 4-MUG (Sigma) starting at 8 weeks of age and maintained on this diet until they were euthanized ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and used the mvrnorm function in the MASS package [43] to estimate 10,000 samples for each G from the multivariate normal distribution (mu, Sigma), where mu is a vector of parameter estimates and Sigma is the inverse of the AI matrix ...
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bioRxiv - Genetics 2023Quote: ... The powder obtained was added to 100 μL chondroitinase ABC solution (50 mM Tris HCl pH 8, 50 mM sodium acetate, 0.1 mU/μL chondroitinase ABC (Millipore)) and incubated in a thermomixer set to 37 °C and 1,500 rpm for 6 h ...
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bioRxiv - Pathology 2023Quote: ... Livers were removed and incubated for 30 min at 37°C under gentle agitation with HBSS containing collagenase D (0.2 mU/mL, Sigma). Cell suspensions were passed through a 100 μm cell strainer before staining ...
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bioRxiv - Neuroscience 2020Quote: PMA and TTX: MAPK pathways were activated intracellularly via PKC with 1µM-1nM phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, #P1585). PMA treatments were applied as a 2° treatment in place of bicuculine ...
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bioRxiv - Biochemistry 2023Quote: ... The suspensions were treated with French Press and added with 5 μM His8-SUMOstar-C3a (homemade) and 25 mU/ml apyrase (Sigma), followed by incubation for 1.5 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Samples were treated with either 500 mU of Phospholipase C (PI-PLC) (Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus; Sigma-Aldrich) prepared according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... With the fluorescent GCase substrates blue fluorogenic substrate 4-methylumbelliferyl-β-D-glucopyranoside (4-MU) (Sigma-Aldrich; as described in 11,12), PBMCs were washed 2x with PBS and lysed in 60μl of activity assay buffer (50mM Citric acid ...