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Citations for Millipore Sigma :
401 - 450 of 1384 citations for African Swine Fever virus pp62 polyprotein His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: HA Tag-FITC (7 ug/mL) (Sigma, clone HA-7)
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-FLAG tag (clone M2-F1804, Sigma, 1:1000), mouse anti-GFP (clones 7.1 and 13.1 ...
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bioRxiv - Biophysics 2021Quote: ... The MBP tag was cleaved with Thrombin (Sigma-Aldrich, USA) at 0.4 unit per mg of protein ...
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bioRxiv - Cell Biology 2022Quote: ... then HRP tags were activated with enhanced chemiluminescence solution (Millipore). Signal was detected and imaged via ChemiDoc™ MP Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... anti-Flag or anti-Pk (V5 Tag) antibodies (Sigma-aldrich) were used for detecting epitope-tagged proteins ...
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bioRxiv - Microbiology 2023Quote: ... which carries the N and C-terminal 6xHistidine tags (Novagen), using the Hi-Fi DNA assembly kit (New England Biosciences).
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bioRxiv - Cell Biology 2022Quote: TAG quantification was performed using Triglyceride Quantification kit (Sigma-Aldrich). For TAG quantification of whole body ...
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bioRxiv - Microbiology 2023Quote: ... An anti-histidine tag antibody (Sigma, St. Louis, MO, USA) and serum from the immunized mice were used as positive controls ...
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bioRxiv - Microbiology 2023Quote: ... or with rabbit primary antibodies against HA-tag (Sigma-Aldrich) followed by anti-rabbit HRP-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-HA-Tag (1:500, rabbit Sigma-Aldrich H3663), anti-FLAG (1:50 ...
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bioRxiv - Microbiology 2024Quote: ... rabbit polyclonal against FLAG tag at 1:500 (F7425, Millipore), rabbit polyclonal against Myc tag at 1:2000 (ab9106 ...
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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Evidence for Involvement of WDPCP Gene in Alcohol Consumption, Lipid Metabolism, and Liver CirrhosisbioRxiv - Genetics 2023Quote: ... were incubated together with either 35 µL of PBST (for free glycerol measurements) or 35 µL of TAG reagent (Sigma, no. T2449, for TAG measurements) at 37 °C for 60 min ...
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iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... 2 ml of 50 % HIS-Select® Cobalt affinity resin (Sigma) was washed with milli-Q water and then with equilibration buffer (20 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... The cell lysate was combined with His-Select Co2+ resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 800 mM) ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 300 mM) ...
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bioRxiv - Microbiology 2021Quote: ... and a combination of α-6×His antibody (Sigma Aldrich, H1029) and HisProbe-HRP (Thermo ...
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bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
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bioRxiv - Plant Biology 2022Quote: PIF3 recombinant proteins were prepared using PIF3-his DNA (pET28C, Novagen) provided by Ferenc Nagy and induced and purified using commercial kit (Amersham) ...
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bioRxiv - Cell Biology 2021Quote: His-ACBD5 was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG overnight at 18°C ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Microbiology 2021Quote: ... Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt, Germany). Rhodamine B isothiocyanate-Dextran (RITC-Dextran ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins were purified using Ni2+-containing His-Bind columns (Novagen). Purified proteins were desalted and concentrated by ultracentrifugation (Amicon ...
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bioRxiv - Cell Biology 2020Quote: ... followed by immunoblotting with HRP-conjugated anti-His antibody (Sigma-Aldrich) by using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067) by overnight incubation at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... –Ade (20 ug/mL L-His HCl monohydrate (A-9126, Sigma) added to –Trp ...
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bioRxiv - Immunology 2021Quote: ... either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich) 0.1M HEPES ...
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bioRxiv - Microbiology 2021Quote: ... probed using 1:500 monoclonal anti-His HRP-conjugated antibodies (Sigma) and imaged using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... the ORF of eggpl was inserted into vector piztv5-His (Novagen) in our laborary to overexpress eggpl ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
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bioRxiv - Biochemistry 2023Quote: ... and supernatant was bound to Ni-NTA His-Bind Resin (Novagen) for 1hr at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was loaded onto Ni-resin (HIS-Select, Sigma-Aldrich) and washed with buffer 1 (50 mM Hepes-KOH pH7.6 ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2019Quote: ... Virus was thawed at 37°C and 5μl 10mg/ml polybrene (Sigma H9268) was added to the virus ...
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bioRxiv - Biochemistry 2021Quote: ... Cholesteryl hemisuccinate (CHS) and tobacco etch virus (TEV) protease were from Sigma-Aldrich.
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bioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated and purified via Iodixanol gradients (“Optiprep” Sigma D1556-250mL). Centrifuge the gradients for 90 min at 46,500 rpm at 16 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... virus containing supernatant was harvested and filtered (0.45 μm, Millex-HV Filter; Millipore). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were infected with virus in the presence of 8μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... Live filtered virus was used to transduce cells with polybrene (10μg/ml, Sigma).
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bioRxiv - Cell Biology 2020Quote: ... the virus was concentrated using Amicon ultra-15 centrifugal filter units (Millipore Sigma) by centrifuging at 3000 rpm and stored at -80°C until use ...
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bioRxiv - Immunology 2019Quote: ... Virus-containing supernatants were filtered through a 0.45-μm-pore-size filter (Millipore) and supplemented with polybrene (8 μg/ ml ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Microbiology 2019Quote: ... virus dilutions were overlaid with 1 ml of Opti-MEM [1% Carboxymethylcellulose (Sigma), 2% FBS ...
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bioRxiv - Microbiology 2021Quote: ... Virus inoculum was removed and cells overlaid with MEM containing 1% carboxymethylcellulose (Sigma), 4% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... 100 μl of virus preparations were treated with 25 units of Benzonase (Sigma) for 30 min before RNA isolation.