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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... β-tubulin antibody and β-actin antibody from Sigma-Aldrich (St. Louis, MO). HRP-linked anti-rabbit IgG ...
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bioRxiv - Immunology 2024Quote: ... β-hexosaminidase substrate (4-nitrophenyl N-acetyl-β-D-glucosaminide, 4 mM, Sigma) was then added to the supernatant and lysate for 1 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: We used TSA plates containing 5% lactose-free, skim milk (Fairlife, LLC) or 0.5% Type II porcine gastric mucin (Sigma Aldrich) to screen the otic isolates for mucinase and protease secretion ...
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bioRxiv - Developmental Biology 2022Quote: ... were routinely cultured at 37°C and 5% CO2 on dishes coated with 0.1% gelatin type B (Sigma Cat3G9391-100G) in GMEM (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were gently minced and digested at 37[°C for 25[minutes with an enzyme cocktail (5 mg collagenase type I with 2.5 mg trypsin, Sigma-Aldrich) in 5 ml DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... EVs were obtained by incubating red blood cell with CaCl (5 mM) and other cell types with the calcium ionophore A23187 (1 mM, C9275, Sigma) for 30 min at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... The 50 mg of type I collagen sponge and 5 ml of PBS (D5652-10L, Sigma–Aldrich, St. Louis, USA) were added into a 15 ml centrifuge tube (Corning ...
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bioRxiv - Immunology 2023Quote: ... Minced pancreas samples from NOD mice were digested in RPMI-10 supplemented with 5 mg/ml Collagenase Type V (Sigma) and 10 ug/ml DNase I followed by incubation in enzyme-free cell dissociation buffer (Sigma ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and then seeded in 6-well plates (5×105 cells/well) that had been pre-coated the day before with type I collagen (Sigma) dissolved in 0.02 N acetic acid ...
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bioRxiv - Biochemistry 2022Quote: ... or −3 human cDNA into a p3XFLAG-CMV-14 expression vector (Sigma, E7908). Then 3XFLAG-PRLs were cloned into pLenti-CMV-puro (Addgene 17452 ...
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bioRxiv - Cancer Biology 2022Quote: ... and CRISPR-Cas9 with a GFP reporter (Sigma Cat# CMV-CAS9-2A-GFP), and flow sorted based on expression of GFP and BFP reporters and absence of CD81/Cd81 ...
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bioRxiv - Molecular Biology 2020Quote: ... H1299 cells were transfected with the p3×FLAG-CMV 10 vector (Sigma-Aldrich) containing human full-length NRF3 or GFP ...
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bioRxiv - Immunology 2020Quote: 3D Collagen type I was prepared using 8 parts rat collagen type I (Sigma) and 1 part 10x DMEM and was neutralized to pH 7.0-7.5 by adding 0.5 parts 0.5N NaOH ...
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bioRxiv - Neuroscience 2021Quote: ... Animals were subsequently administered 5 μg 17-β-estradiol benzoate in 50uL sesame oil (E2; Sigma-Aldrich, St. Louis, MO) on Day 2 ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% glycerol and 2 mM β-mercaptoethanol) supplemented with 1 pill of cOmpleteTM EDTA-free Protease Inhibitor cocktail (Sigma-Aldrich). Cells were then sonicated and the lysate was clarified by centrifugation in a Beckman JA-25.50 rotor at 50000 x g for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 mg/mL X-Gal (5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside, Sigma Aldrich Aldrich, St. Louis, MO), and 150mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... OVX females received 5 μg 17-β-estradiol benzoate in 5uL sesame oil (E2, SC; Sigma Aldrich, St. Louis, MO) followed by 10μg E2 in 10μL sesame oil 24 h later ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were prepared so that they were at a concentration of 20-30µg and 6X Laemmli sample buffer containing 5% β-mercaptoethanol (Sigma) was added ...
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bioRxiv - Immunology 2021Quote: ... Lung & spleen cells were diluted to 1×106cells/ml before incubating with 50 μg/ml SARS-CoV-2 purified spike antigen for 24 h in media (RPMI-1640, 10% FCS, 100U/ml penicillin/streptomycin, 5%NEAA, L-glutamine and HEPES, 0.05 mM β-mercaptoethanol (SIGMA)) ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM β-mercaptoethanol and 5% glycerol was mixed gently with 20 µL of 1 M dimethylamine-borane complex (Sigma) per 1 mL of protein solution ...
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bioRxiv - Molecular Biology 2022Quote: ... was added to a final concentration of 1% and incubated for 5 minutes at 37°C shaking at 800 rpm and the reaction quenched with 60µl β-mercaptoethanol (Sigma). DMS modified RNA was purified using RNA Clean and Concentrator-5 kit and eluted in 10 ul water ...
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bioRxiv - Microbiology 2023Quote: ... and pretreated 1 h at 37°C with either 5 mM Methyl-β-cyclodextrin (MBCD) (Sigma Aldrich, stock in ddH2O) in OptiMEM medium buffered with 25 mM HEPES (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... staining solution (0.3 mg/ml of 5-bromo4-chloro-3indolyl β-D-galactoside, X-Gal Fermentas R0401, 40 mM citric acid, Sigma C-0759 ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was mixed with about 10 time molar excess of guanosine 5′- [β,γ-imido]triphosphate (GMMPPNP, SIGMA G0635) or guanosine 5’-triphosphate (GTP ...
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bioRxiv - Plant Biology 2024Quote: ... meliloti 1021 (pXLGD4) strain were stained using 5-bromo-6-chloro-3-indolyl-β-d-galactopyranoside (Magenta-Gal, Sigma-Aldrich), according to the protocol described previously by Jarzyniak et al ...
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bioRxiv - Cell Biology 2023Quote: ... primary myoblasts were transduced with both hTERT and Cdk4 lentiviral vectors with a ratio of the number of transducing lentiviral particles to the number of cells (MOI) of 5 in the presence of 4 μg/ml of polybrene (Sigma-Aldrich, Sant Luis, MO, USA). Transduced cell cultures were selected with puromycin (0.2 μg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... protease Type I (Sigma-Aldrich) was used to digest the cardiac tissue for 10 min ...
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bioRxiv - Immunology 2019Quote: ... collagenase type XI (Sigma, C7657), and collagenase type II (Sigma ...
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bioRxiv - Immunology 2019Quote: ... collagenase type IV (Sigma, C5138) at a final concentration of 1 mg/ml ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... collagen type IV (Sigma-Aldrich), and fibronectin (Gibco) ...
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bioRxiv - Physiology 2020Quote: ... Collagenase (type V, Sigma Aldrich) was injected into the pancreas through the pancreatic duct ...
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bioRxiv - Bioengineering 2021Quote: ... collagen type IV (Sigma C5533) and fibronectin (Sigma 1141 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Untagged wild-type Ub (Sigma) was resuspended in maintenance buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... and DNAse type IV (Sigma) for 60 min at 37°C with constant shaking ...
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bioRxiv - Biochemistry 2022Quote: ... Collagen type IV (Sigma C5533) was coated on 96 well plates and left for 6 hours or more before using the plates whenever seeding PC-12 cells.
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bioRxiv - Immunology 2023Quote: ... Collagenase type IV/DNaseI (Sigma) was added ...
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bioRxiv - Neuroscience 2023Quote: ... and collagenase type 2 (Sigma), then mechanical dissociated by trituration ...
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bioRxiv - Cancer Biology 2023Quote: ... DNase I Type 4 (Sigma), accutase (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... collagenase type XI (Sigma, D9542) 900 uni/ml ...
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bioRxiv - Physiology 2023Quote: ... and protease type XIV (Sigma). The SAN region ...
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bioRxiv - Physiology 2023Quote: ... and protease type XIV (Sigma) for 20-30 min ...
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bioRxiv - Neuroscience 2023Quote: ... and collagenase type 2 (Sigma), and mechanically with trituration ...
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bioRxiv - Immunology 2023Quote: ... and DNase type IV (Sigma) for 1 h at 37°C under constant agitation ...
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bioRxiv - Microbiology 2023Quote: ... type IX-S (Sigma-Aldrich), per ml for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: μM particle size 10 mm × 25 cm) column (Sigma Aldrich). To prepare initiation complexes ...
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bioRxiv - Cancer Biology 2021Quote: ... were transduced with ready-made Mission shRNA lentiviral particles (Sigma) expressing four different LBH-specific shRNAs (TRCN0000107525-shLBH#1 ...
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bioRxiv - Microbiology 2021Quote: ... using Magnetic Carboxylate Modified Particles beads (Sigma-Aldrich, cat.no.45152105050250). Protein digestion was performed on beads using Trypsin Gold (MS grade ...
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bioRxiv - Microbiology 2022Quote: NOD2 MISSION shRNA Lentiviral Transduction Particles from Sigma Aldrich (TRCN0000066813) were used to stably down-regulate NOD2 in J774 macrophage ...
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bioRxiv - Cell Biology 2021Quote: Ready-to-use lentiviral particles were purchased from Sigma-Aldrich. hCMEC/D3 cells were transduced first by lentiviral particles expressing Cas9 and blue fluorescent protein (BFP ...
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bioRxiv - Cell Biology 2021Quote: ... and viral particles were mixed with polyethylene glycol 8000 (SIGMA) to a final concentration of 5-6% and incubated at 4°C for 5 hr ...