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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for 7 Oxa 12 thia spiro 5.6 dodecan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Dead cells were excluded with 7-aminoactinomycin D (7-AAD, Sigma). Cell were either sorted on AriaII or AriaIII cell sorter (BD Biosciences ...
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bioRxiv - Physiology 2021Quote: ... 12 week-old mice were fed ad libitum and given CL316243 (3 mg/kg body weight; Sigma Aldrich) or vehicle (0.5% carboxymethylcellulose in sterilized water ...
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bioRxiv - Cancer Biology 2020Quote: PC-3 cells (RRID:CVCL_0035; obtained from ATCC) were cultured in F-12 Kaighn’s Modification HAM medium (Sigma Aldrich) containing 10% heat-inactivated Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: The chemical (2S, 3S)-1,4-Bis-sulfanylbutane-2,3-diol (DTT, CAS No. 3483-12-3) was obtained from Sigma; it is a strong reductant with chemical formula of C4H10O2S2 and MW 154.25g/mol ...
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bioRxiv - Bioengineering 2022Quote: ... Bis-cysteine crosslinking peptide VPM (GCNSVPMSMRGGSNCG) or non-degradable crosslinking agent DTT (1,4-dithiothreitol; Sigma, 3483-12-3) was dissolved in diH2O at 27.3 mM ...
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bioRxiv - Bioengineering 2023Quote: Monocytes were activated to macrophages by adding 30ng/mL phorbol-12-myristate-3-acetate (PMA; Sigma-Aldrich: P8139) for 24hr ...
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bioRxiv - Microbiology 2020Quote: ... parahaemolyticus cells incubated in ASW microcosms at 4°C for 100 days were re-suspended in 50 mM potassium phosphate buffer (pH 7) containing 1 g of 3-mm-sized glass bead (Sigma), vortexed for 25 min ...
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bioRxiv - Bioengineering 2022Quote: ... All primary cells were used between subculture 3–7 and were routinely cryopreserved in 90% human serum (#H4522; Sigma-Aldrich) supplemented with 10% dimethyl sulfoxide (DMSO).
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bioRxiv - Biochemistry 2019Quote: The custom designed CompoZr® Zinc Finger Nuclease Plasmid targeted to exon 3 of the mouse ECH1 gene on chromosome 7 was obtained from Sigma. In-vitro transcription of the ZFN mRNA was performed following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... samples were resuspended in Urea 7 M and Thiourea 2 M buffer and desalted on Amicon Ultra-0.5 3 kDa centrifugal filters (Merck Millipore, Germany). Filters were filled to maximum capacity with buffers and centrifuged at 15,000 g for 10 min at 20°C ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 10μM 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (18:1 NBD-PS) (1:300, Sigma-Aldrich) for 15 minutes at 25°C ...
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bioRxiv - Immunology 2020Quote: Each peptide sample was desalted on Empore SPE C18 cartridges (standard density, bed inner diameter 7 mm, volume 3 ml; Sigma), then concentrated by vacuum centrifugation and reconstituted in 40 µl 0.1% (v/v ...
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bioRxiv - Plant Biology 2023Quote: ... The 30 collected fractions were combined into 15 pools and desalted on C18 Cartridges (Empore SPE Cartridges C18 standard density, bed I.D. 7 mm, volume 3 ml, Sigma-Aldrich). Each fraction was concentrated by vacuum centrifugation and reconstituted in 40 µl of 0.1% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged at a 1:3 to 1:6 ratio every 7 days using cell dissociation solution-non enzymatic (Sigma) and plated in fresh BME matrix droplets.
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bioRxiv - Cell Biology 2019Quote: ... Single colonies were picked and transferred to 12 well plate (one colony per well) using trypsin-soaked Scienceware® cloning discs (Sigma. Dorset, UK). Cells were grown until confluent and then transferred to 25 cm2 flasks ...
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bioRxiv - Neuroscience 2020Quote: ... A 96-well PCR plate was prepared with one 3 mm diameter borosilicate solid-glass bead (Millipore Sigma #Z143928) and 100 µl PBS in each well ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by a one-hour blocking step using 0.1% triton-X with 3% Bovine Serum Albumin (BSA, Sigma A7906) in PBS ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were then stained with 7-Aminoactinomycin D (7-AAD, Sigma-Aldrich) and an AF488-conjugated anti-KI-67 antibody (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... CM metabolite 7-hydroxycoumarin (7-HC; Cat. No. H24003, Sigma-Aldrich Corp.), BFC ...
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bioRxiv - Neuroscience 2020Quote: ... 7 MgCl2 (Sigma-Aldrich), 0.5 CaCl2 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... pH=7 (Sigma, USA). After 10 hr ...
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bioRxiv - Developmental Biology 2022Quote: ... 4% 7-DHC (Sigma)) and 50-200 μL fresh baker’s yeast as food source and egg laying substrate on top ...
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bioRxiv - Microbiology 2021Quote: ... containing 7% BSA (Sigma), 1x penicillin streptomycin (Gibco ...
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bioRxiv - Immunology 2019Quote: ... 5Z-7-Oxzeaenol (Sigma).
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bioRxiv - Microbiology 2020Quote: ... (HA-7; H9658, Sigma) and GAPDH (SCBT ...
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bioRxiv - Cancer Biology 2021Quote: ... 7-AAD dye (Sigma) was used to differentiate live and dead cells ...
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bioRxiv - Neuroscience 2022Quote: ... 7 μM Dorsomorphin (Sigma) and 10 μM TGFβ inhibitor SB431542 (MedChem express ...
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bioRxiv - Neuroscience 2022Quote: ... 7 μM Dorsomorphin (Sigma) and 10 μM TGFβ inhibitor SB431542 (MedChem express ...
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bioRxiv - Neuroscience 2024Quote: ... 7 MgCl2 (Sigma-Aldrich), 0.5 CaCl2 (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: ... pH = 5.6) and resuspended in lysing solution (15 ml buffer A containing 150 mg lysing enzymes from T. harzianum (Sigma-Aldrich, St. Louis, MO, USA, L1412) and 150 mg β-glucanase from T ...
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bioRxiv - Bioengineering 2023Quote: ... one Asymptote Freezer (Grant) and one Sigma 6K15 Centrifuge (Sigma), all inside a BSL2 sterile enclosure ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 12 mg RBD protein in 3 mL PBS was mixed with equal-volume Freund’s incomplete adjuvant (Sigma-Aldrich) for the second boosting ...
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bioRxiv - Immunology 2022Quote: ... 7.5×107/15ml RPMI+3%FBS and stimulated with 25nM phorbol 12-myristate 13-acetate (PMA; Millipore-Sigma #P8139) Plates were incubated at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 7.5×107/15ml RPMI+3%FBS and stimulated with 25nM phorbol 12-myristate 13-acetate (PMA; Millipore-Sigma #P8139) Plates were incubated at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The infected host cells were incubated for 7 min at 37°C with DMEM containing 3 μM of calcium ionophore A23187 (C7522, Sigma-Aldrich) prior to fixation with 4% PFA ...
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bioRxiv - Bioengineering 2022Quote: ... The retinal strips were sectioned (7–10 µm thick) and placed on microscope slides that were treated with 3-Aminopropyltriethoxysilane (APES, Sigma A3648). Before immunohistochemistry ...
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bioRxiv - Plant Biology 2021Quote: ... Lipids extracts were incubated 1 h at 50°C in 2 mL of methylamine solution (7 ml methylamine 33% (w/v) in EtOH combined with 3 mL of methylamine 40% (w/v) in water (Sigma Aldrich) in order to hydrolyze phospholipids ...
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bioRxiv - Cancer Biology 2020Quote: ... and the pellet resuspended and incubated for 3-7 minutes at 37°C in accutase dissociation reagent (Sigma, Poole, UK, A6964). After enzyme neutralization with fresh medium ...
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bioRxiv - Cell Biology 2019Quote: ... 100 nM ALK2/3 inhibitor LDN193189 (Miltenyi Biotech, 130-106-540) and 10 uM ALK4/5/7 inhibitor SB431542 (Millipore, 616464). Medium was replaced on days 1 and 2.
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bioRxiv - Immunology 2021Quote: ... that contain two parallel channels separated by a porous membrane (7 μm pores) were first perfused and incubated with 1% 3-Aminopropyltrimethoxysilane (Sigma, 281778) in ethanol for 1 hour and then incubated in an 80°C oven overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Myelin and debris removal was performed by a gradient centrifugation of the cell suspension in a 7:3 mix of PBS:Percoll (Sigma-Aldrich, #P4937), with an additional PBS wash ...
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bioRxiv - Molecular Biology 2024Quote: ... containing a sgRNA sequence (5’-TCATCGCTCACAACCAAGTG-3’) targeting exon 7 of ERBB2 (cloned by Twin Helix) and selected with puromycin (Sigma-Aldrich) for 4 days (1 μg/ml puromycin for 72 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Cre-mediated recombination was induced in mice 3-7 months of age by intraperitoneal injection of 2 mg/day tamoxifen (Sigma-Aldrich) diluted with 300 μl corn oil (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Michigan Cancer Foundation 7 (MCF-7) cells were cultured with DMEM (Sigma D6429) with 10% foetal bovine serum (FBS) ...
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bioRxiv - Biophysics 2020Quote: ... the following target sequences were used: 5’-GCTTCAGGATTCAATGCCATGG-’3 (#1) using the all-in-one CRISPR/Cas9 plasmid (Sigma-Aldrich) followed by cell-sorting for GFP expression to generate single cell clones with disrupted ANXA4 reading frame.
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bioRxiv - Genetics 2020Quote: A 32P 5′-labeled 70-mer oligonucleotide [5′-42(T)-ATCTCAGCGATCTGTCTATTTCGTTCAT-3′] was hybridized to a single-stranded pBluescript SK(+) followed by one cycle of polymerization using KOD polymerase (Novagen) to produce a ∼3-kb double-stranded template with a preformed replication fork ...
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bioRxiv - Cell Biology 2021Quote: ... were reared in 10g/l of glucose/200µM of 3BDO (3-Benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one) (Sigma Aldrich) supplemented in standard fly food ...
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bioRxiv - Synthetic Biology 2021Quote: ... Eluted protein samples were combined in one tube and desalinated using an ultrafiltration tube (Amicon Ultra 3 kDa molecular weight cut-off, Merck/Millipore) with Buffer C (50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Cell Biology 2022Quote: ... and transferred to 1.5 ml Eppendorf tubes where the cells were fixed for a minimum of one hour or overnight in 3% formaldehyde (Sigma) in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... Blots were washed an additional three time with TBS-T and one time with TBS before 3 minute exposure with ECL HRP substrate (Millipore) and imaged on a BioRad ChemiDoc system ...