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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for 7 Chloro 2 4 bis trifluoromethyl 1 8 naphthyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Buffer exchange into TBS pH7.4 and 1 mg/ml Amphipol 8-35 using Amicon Ultra-4 centrifugation filters with a 100kDa cutoff (Millipore). The concentration of Amphipol 8-35 was brought up to 35mg/ml and incubated at room temperature for 4 hours then Bio-Beads SM-2 (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: Cells were incubated with 10 µM 2′,7′-dichlorofluorescein (DCFDA) (Sigma, USA) in PBS for 30 min in dark ...
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bioRxiv - Molecular Biology 2022Quote: ... serially diluted from 10-7 to 10-2 in PBS (Sigma®) and plated onto BHI agar plates ...
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bioRxiv - Cell Biology 2021Quote: ... and 2’,7’-dichlorofluorescin diacetate (DCF-DA) (35845) were purchased from Sigma. Guinea pig anti-rabbit IgG (Heavy & Light Chain ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2’,7’ dichlorofluorescein diacetate (DCFH-DA) were procured from Sigma-Aldrich (USA). We purchased the nitro blue tetrazolium (NBT ...
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bioRxiv - Plant Biology 2019Quote: ... The antigen–antibody complexes were visualized using nitrotetrazolium blue chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) buffer (Sigma-Aldrich, St. Louis, Missouri, USA) under standard conditions ...
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bioRxiv - Plant Biology 2023Quote: ... 2 mM Ca(NO3)2 ×24H2O (13477-34-4, Sigma), 2.5 mM KH2PO4 (7778-77-0 ...
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bioRxiv - Biochemistry 2023Quote: ... glucose (G8270) and β-chloro-Lalanine (C9033) were purchased from Sigma-Aldrich. Potassium phosphate monobasic (42420 ...
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bioRxiv - Developmental Biology 2022Quote: ... Single mESC clones were picked 7-8 days after transfection and plated onto 96-well synthemax (Sigma, #CLS3535) coated plates and screened for genomic DNA deletion by PCR using primers outside of the deletion region.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 8 μM hemin (4 mM stock made in 50% triethanolamine) (Sigma, USA), 25mM Hepes (N-[2-hydroxyethyl]piperazine-N0-[2-ethanesulfonic acid ...
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bioRxiv - Cell Biology 2022Quote: ... The medium was removed and replaced with 1 mL of BI-S-33 supplemented with either 2 mg/mL RITC-dextran (MW = 70 000; Sigma-Aldrich, USA) or 100 μg/ml Alexa Fluor-568 transferrin (Thermo-Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in PBS containing 0.5% normal donkey serum and 0.03% Triton X-100 for 1 h at room temperature (RT, 23 ± 7□) and then stained with primary antibodies overnight at 4□ (rabbit anti-c-Fos, 1:5000, Sigma-Aldrich; rat anti-CTIP2 ...
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bioRxiv - Cell Biology 2022Quote: ... diluted with fresh medium (1/2) and hexadimethrine bromide was added (final concentration 8 μg/ml; Sigma-Aldrich). Target cells were then infected ...
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bioRxiv - Neuroscience 2023Quote: ... 7 nl ml−1 β-mercaptoethanol (Sigma-Aldrich), 1% L-glutamine (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... spleens were fixed for 2 h at 4°C in 2 mL of 4% paraformaldehyde (Millipore Sigma) followed by an overnight incubation at 4°C in 2 mL of 30% sucrose (Millipore Sigma) ...
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bioRxiv - Immunology 2022Quote: ... cells were transduced by adding ~1-2 ml of RV supernatants supplemented with 50 μM 2-βME and 8 μg/ml polybrene (Millipore, Cat # TR-1003-G), followed by centrifugation for 90 min at 524g at 37 °C ...
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bioRxiv - Biophysics 2024Quote: ... which was prepared with 20 mM HEPES-buffer (2-(4-(2-Hydroxyethyl)-1- piperazinyl)-ethansulfonsäure) (Sigma-Aldrich, ≥99.5 %, H3375) as calcium chloride is not soluble in DPBS-buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... for 2 h or 4-hydroxytamoxifen (4-OHT, Sigma, 10 µM) for 4 h ...
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bioRxiv - Genomics 2020Quote: ... Ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA, Sigma-Aldrich, E4378) and calcium chloride dihydrate (VWR ...
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bioRxiv - Cell Biology 2021Quote: H2S production was measured using 7-Azido-4-Methylcoumarin (AzMC) fluorescent dye (Sigma, #802409). The protocol was adapted from Szabo Laboratory with minor modifications (Modis et al. ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM 4-(2-Aminoethyl)-benzenesulfonylfluoride hydrochloride (AEBSF) and 1 complete ULTRA protease inhibitor tablet (Sigma-Aldrich)) then clarified by centrifugation at 14,000 x g for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were irradiated using 8 J/m2 UV and labelled for 7 hours with 20 µM 5-Ethynyl-2’-deoxyuridin (EdU) and 1 µM Floxouridine (Sigma). Subsequently ...
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bioRxiv - Biophysics 2023Quote: ... the acrylate functionalized photodegradable monomer was synthesized by suspending 4-[4-(1-hydroxyethyl)-2-methoxy-5-nitrophenoxy]butyric acid (0.0166 mol, Sigma-Aldrich) in anhydrous DCM (90 mL) ...
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bioRxiv - Biophysics 2019Quote: ... 2 mM MgSO4 (Sigma Aldrich, cat# 63138, CAS:10034-99-8)) with supplements for deficient folate metabolism (50 µg/mL thymidine (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... LabTek 8-well plates and treated with 2 mM thymidine (Sigma) and 2 μg/ml doxycycline for 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 CV of wash buffer 2 (40 mM Tris pH 8, 300 mM NaCl, 20 mM imidazole pH 8, 2.15% sodium cholate (Sigma), 1% Triton X-100) ...
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bioRxiv - Molecular Biology 2023Quote: ... passed through a 0.45 µm filter and used to infect either HEK293 cells in a dilution of 1:5 or RAW macrophages in a dilution of 1:2 with 8 µg/ml polybrene (Sigma-Aldrich, TR1003G) containing standard cultivating medium ...
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bioRxiv - Bioengineering 2020Quote: ... 7-hydroxycoumarin (7-HC, Sigma-Aldrich), generated from coumarin was quantified via fluorescence detection (excitation/emission ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI) (1:3000; Sigma-Aldrich) and coverslips were mounted with FluoroGel (Electron Microscopy Services ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1:10000; Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2022Quote: ... Triple Dynamin (Dyn1/2/3) knockout was induced by 1 μM 4-hydroxytamoxifen (Sigma) as previously described44 ...
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bioRxiv - Neuroscience 2021Quote: DOI or 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane was obtained from Sigma-Aldrich. Vehicle refers to saline (0.9%) ...
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bioRxiv - Physiology 2019Quote: ... Nuclei were stained with DAPI (1:1000; 4’,6-diamidino-2-phenylindole dihydrochloride; Sigma).
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bioRxiv - Cell Biology 2020Quote: ... 4′,6-diamidino-2-phenylindole (DAPI, Sigma #D9542, 1:1000 for 10min at RT) Mice were deeply anesthetized with isoflurane and then transcardially perfused with ice-cold heparinized PBS (25U/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... slices were incubated with 4′,6-diamidino-2-phenylindole (DAPI; 1:500, Sigma-Aldrich) to counterstain cell nuclei ...
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bioRxiv - Neuroscience 2022Quote: ... After staining nuclei with DAPI (1:1000, 4’, 6-diamidino-2-phenylindole dihydrochloride; Sigma) and several washes with PBST ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI) (1:3000; Sigma-Aldrich), and coverslips were mounted with FluoroGel (Electron Microscopy Services ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained for 1 hour at 4 °C with 2% rat serum (Sigma) to reduce antibody binding to Fc receptors ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI) (1:3000; Sigma-Aldrich) and coverslips were mounted with FluoroGel (Electron Microscopy Services ...
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bioRxiv - Molecular Biology 2020Quote: ... A total of 55 million Jurkat cells seeded at 8 million cells ml-1 was treated with 50 µM of 4-thiouridine (Sigma) for 7 hours ...
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bioRxiv - Immunology 2022Quote: ... or the ACE2 product Ang (1-7) (Sigma, #A9202; 1 μM) in the same culture conditions described above.
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bioRxiv - Developmental Biology 2019Quote: ... adding Hoechst 33258 (bis-Benzimide H, 1: 10,000 dilution; Sigma-Aldrich) during the first wash to stain nuclei ...
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bioRxiv - Biochemistry 2020Quote: ... O-Bis (trimethylsilyl) trifluoroacetamide (BSTFA) with 1% trimethylchlorosilane (TMCS) (Sigma-Aldrich) 70°C for 30min ...
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bioRxiv - Cancer Biology 2020Quote: ... nuclei were stained with bis-benzamide (1 mg/mL; Sigma-Aldrich) for 7 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... nuclei were stained with bis-benzamide (1 mg/mL; Sigma-Aldrich) for 7 min at RT ...
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bioRxiv - Neuroscience 2019Quote: ... separated on 4-12% Bis-Tris mini gel (Nupage) and transfered to a PVDF membrane (Millipore #ISEQ00010). Nonspecific binding was blocked for 1 h at room temperature with 5% blotto (Biorad ...
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bioRxiv - Neuroscience 2020Quote: ... separated on 4-12% Bis-Tris Mini Gel (NuPAGE) and transferred to a PVDF membrane (Millipore #ISEQ00010). Nonspecific binding was blocked for 1 hr at RT with 5% BLOTTO (Bio-Rad ...
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bioRxiv - Synthetic Biology 2022Quote: ... and SDS-PAGE gels were run in a 4-20 % Bis-Tris polyacrylamide precast gel (Sigma Aldrich). Samples were not heated to avoid denaturing GFP ...
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bioRxiv - Synthetic Biology 2024Quote: ... the SDS-PAGE gel was run in a 4–20% Bis-Tris polyacrylamide precast gel (Sigma Aldrich). The gel was stained using SimplyBlue stain (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... 200 μl of N,O-Bis(trimethylsilyl)trifluoroacetamide+1% trimethylsilyl (BSTFA+1% TMCS, Sigma) were added and incubated at 110 °C for 15 min ...