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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for 7 Bromo 4 chlorothieno 3 2 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... inhibitor 2-bromohexadecanoic acid (2-bromo-palmitate; 2-Br) [IC50 of ∼4 µM] (Varner et al., 2003) were purchased from Sigma-Aldrich (St. Louis, MO). Bradford protein assay kit as well as anti-mouse and anti-rabbit secondary antibodies were obtained from Bio-Rad (Hercules ...
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bioRxiv - Cancer Biology 2020Quote: ... and 7-amino-actinomycin D (7-AAD; Sigma Aldrich) were added for 15 minutes at room temperature in the dark ...
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bioRxiv - Synthetic Biology 2023Quote: ... 100 μL of 1– bromo–3–chloropropane (Sigma Aldrich, B9673-200ML) was added to the cell lysate ...
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bioRxiv - Bioengineering 2023Quote: ... Phase separation was performed with 1-bromo-3-chloropropane (Sigma Aldrich) and the aqueous phase was purified using RNeasy micro spin columns ...
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bioRxiv - Developmental Biology 2023Quote: ... A volume of 200 μl 1-bromo-3-chloropropane (Sigma Aldrich) was added and the mixture was incubated for 10 min at room temperature before centrifugation (10 min at 10.000 x g) ...
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bioRxiv - Microbiology 2021Quote: ... 7-aminoactinomycin D (Sigma Aldrich) were included during sample preparation according to the manufacturer’s instructions to identify dead cells.
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated with nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) substrates (Sigma-Aldrich) in NTM (100mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and the blotting signals were chemically visualized with either the nitro-blue tetrazolium/5-bromo-4-chloro-3′-indolyphosphate (NBT/BCIP) chromogenic assay (Sigma) or infrared scanner ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... coli DH5α cells were transformed with the resulting pho-lac fusion plasmids and streaked on dual indicator plates containing LB agar with 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos) (Sigma) at a final concentration of 80μg/ml and 6-chloro-3-indolyl-β-D-galactoside (Red-Gal ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were detected by the addition of BCIP (5-bromo-4-chloro-3-indolylphosphate)-nitroblue tetrazolium solution (Sigma-Aldrich). The reaction was stopped after 2 min by washing the blots with large volumes of deionized water.
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bioRxiv - Bioengineering 2021Quote: ... slides were either treated with SIGMA FAST™ BCIP/NBT (5-Bromo-4-chloro-3-indolulphosphate/Nitro blue tetrazolium, pH 9.5, Sigma) and counterstained with nuclear fast red (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... Color was developed with freshly prepared 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT; Sigma-Aldrich) and the reaction was stopped by addition of 2 mM ethylenediaminetetraacetic acid (EDTA).
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bioRxiv - Microbiology 2023Quote: ... the blots were washed again using TBST and developed using AP-reactive Nitrobluetetrazolium (NBT) 5-bromo-4-chloro-3-indolylphosphate (BCIP) tablets (Sigma). A final image of the blots was taken on a Bio-Rad ChemiDoc gel imaging system using colorimetric detection.
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated in NBT (nitro-tetrazolium blue)-BCIP (5 bromo-4-chloro-3’-indolyphosphate p-toluidine) (Sigma-Aldrich), 0.033% in APTMg in dark condition ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed and resuspended in PBS 4%BSA with viability staining reagent 1μg/ml (7-AAD) 7-aminoactinomycin D (Sigma Aldrich). Stained cells were analyzed with a MACSQuant 10 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed and resuspended in PBS 4%BSA with viability staining reagent 1μg/ml (7-AAD) 7-aminoactinomycin D (Sigma Aldrich). Stained cells were analyzed with a MACSQuant 10 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-Bromo-2’-deoxyuridine (BrdU) was purchased from Sigma-Aldrich, dissolved in water ...
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bioRxiv - Cell Biology 2020Quote: ... 5-bromo-2-deoxyuridine (BrdU) were acquired from Sigma Aldrich. Sulfosuccinimidyl 6-(4’-azido-2’-nitrophenylamino ...
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bioRxiv - Pathology 2020Quote: ... and 5-bromo-2-deoxyuridine (BrdU, 100 μmol/L, Sigma). Prior to treatment ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by 100μL of 5-bromo-2’-désoxyuridine (BrdU, Sigma) at 10 mg/mL 90 minutes later ...
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bioRxiv - Neuroscience 2022Quote: ... mice were given 5-Bromo-2’-deoxyuridine (BrdU) (Sigma, B5002). BrdU was dissolved in 0.9% saline at a concentration of 10 mg/mL ...
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bioRxiv - Immunology 2023Quote: Cell cycle analysis: 5-Bromo-2’-deoxyuridine (BrdU, Sigma Aldrich) staining was performed as described previously94.
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bioRxiv - Molecular Biology 2021Quote: ... 3 mM 4-MU N-acetyl-β-D-glucosaminide (Sigma Aldrich) in 200 mM sodium citrate buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 mM 4-MU N-acetyl-β-D-glucosaminide (Sigma Aldrich) in 200 mM sodium citrate buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... or MG132 (10 μM, 2 h, replicates 2, 3, 4; Sigma). Proteins were precipitated in acetone ...
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bioRxiv - Neuroscience 2020Quote: ... The signal was determined by nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) (BCIP) (Sigma, 72091).Semithin sections for toluidine blue myelin staining and ultrathin section for TEM imaging were prepared according to our previous protocols (Zhang et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... at 60°C and developed using nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) (Sigma Aldrich) for 60 minutes ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of the DIG-labelled probes was carried out by using an anti-DIG antibody coupled with alkaline phosphatase and a ready-to-use 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro-blue tetrazolium (NBT) solution (Sigma-Aldrich) as the chromogenic substrate for the enzyme ...
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bioRxiv - Developmental Biology 2020Quote: ... The staining reactions lasted between 2h and 8h at room temperature using nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3′-indolyphosphate (BCIP) staining reactions (Sigma Aldrich). Embryos were then rinsed in PBS-T (0.05% Tween-20) ...
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bioRxiv - Microbiology 2022Quote: ... Detection was performed by colorimetry using nitro blue tetrazolium chloride / 5-bromo-4-chloro-3-indolyl-phosphate (NBT-BCIP, Sigma-Aldrich) as a substrate.
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bioRxiv - Molecular Biology 2019Quote: ... Tissue lysates were mixed with 1-bromo-3-chloropropane (B9673, Sigma-Aldrich) at a ratio of 1:4 and the RNA containing aqueous/organic phase was separated using 5Prime PhaseLock Gel Heavy (#2302830 ...
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bioRxiv - Developmental Biology 2022Quote: ... 4% 7-DHC (Sigma)) and 50-200 μL fresh baker’s yeast as food source and egg laying substrate on top ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were sacrificed under anesthesia with 2-bromo-2-chloro-1,1,1-trifluoro-ethane (Sigma) by cervical dislocation ...
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bioRxiv - Developmental Biology 2020Quote: ... or 1000 nM of 2-(4-amino-1-isopropyl-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-1H-indol-5-ol (PP242; Sigma, P0037), (2 ...
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bioRxiv - Biophysics 2024Quote: ... and 3% (wt/vol) hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Sigma, 410896) in ethanol ...
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bioRxiv - Cell Biology 2023Quote: ... Dead cells were excluded with 7-aminoactinomycin D (7-AAD, Sigma). Cell were either sorted on AriaII or AriaIII cell sorter (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... BrdU (5-Bromo-2’-doxyuridine, Sigma Aldrich, St. Louis, Missouri, USA) was injected intraperitoneally at 100 mg/kg in sterile PBS 24 hours prior to sacrifice ...
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bioRxiv - Developmental Biology 2022Quote: Mice were administered 5-bromo-2′-deoxyuridine (BrdU, Sigma-Aldrich B5002) reconstituted in sterile 1x PBS by intraperitoneal injection at a dose of 50 mg/kg ...
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bioRxiv - Neuroscience 2022Quote: ... 5-bromo-2-deoxyuridine (BrdU; 100 mg/kg; i.p., Sigma-Aldrich) was injected daily for five consecutive days.
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bioRxiv - Immunology 2022Quote: For in vivo labeling with 5-bromo-2’-deoxyuridine (BrdU; Sigma), BrdU was given in drinking water (0.8mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... adding 10 μg/ml 5-bromo-2′-deoxyuridine (BrdU, Sigma-Aldrich) 1 hour before fixation ...
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bioRxiv - Neuroscience 2024Quote: Mice were injected with 5-bromo-2’-deoxyuridine (BrdU, Millipore Sigma) reconstituted in sterile PBS (10mg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... The bound probe was visualized using 5-bromo-4-chloro-3 indoyl phosphate (NBT/BCIP; Sigma-Aldrich Corp., St. Louis, MO, USA). Bright field images were captured on the A1+ Nikon confocal microscope fitted with a DS-Ri1-U3 color camera at 20x magnification and 4076×3116 pixel resolution ...
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bioRxiv - Plant Biology 2020Quote: ... Detection was performed by adding 200 μl of western blue substrate (SIGMAFAST™ BCIP®/NBT = 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium, Sigma) followed by incubation at room temperature in a dark and moist environment until the reagent’s color became visible ...
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bioRxiv - Bioengineering 2022Quote: 4-Nitrophenyl β-D-glucuronide (4-NPG, CAS no. 10344-94-2) (Sigma-aldrich) was prepared in 50 mM sodium phosphate buffer (Na-PB ...
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bioRxiv - Molecular Biology 2023Quote: ... Phase separation was performed by adding 100 μL 1-Bromo-3-chloropropane (Sigma) to 1 mL of homogenate ...
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bioRxiv - Bioengineering 2023Quote: ... The 1.5 ml resuspended RBCs were cultured in 50 ml HPS containing 2 mM CaCl2 and 2 µM calcium ionophore-4-bromo-A23187 (C7522, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) in T75 flasks in a 5% CO2 incubator at 37°C for 16 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bixafen, (N-(3’,4’-dichloro-5-fluorobiphenyl-2-yl)-3-(difluoromethyl)-1-methylpyrazole-4-carboxamide) (Bixafen, (Sigma-Aldrich, St ...
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bioRxiv - Molecular Biology 2023Quote: ... Bromo-chloropropane (Sigma) was added to the lysed worms and centrifuged at 12,000 x g for 15 minutes ...