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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for 7 3 Chloropropoxy 4 chloroquinazoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: Total RNA from 7 DAP spikes was extracted using TRI Reagent and 1-bromo-3-chloropropane (BCP) (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Dried lipids were resuspended in 15µL chloroform and 65µL of click mix containing 400µM 3-Azido-7-hydroxycoumarin (Baseclick #BCFA-047-1) and 900µM tetrakis(acetonitrile)copper(I) tetrafluoroborate (Sigma #677892) in acetonitrile/ethanol (7:3 ...
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bioRxiv - Systems Biology 2020Quote: ... and cells were resuspended in 7 mL of ice-cold 4% formaldehyde (F8775, Millipore Sigma, St. Louis, MO) in PBS (P0195 ...
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bioRxiv - Microbiology 2022Quote: ... and leucine-aminopeptidase using a 7-amino-4-methylcoumarin (AMC)-substrate (L-leucine-AMC, Sigma-Aldrich, Darmstadt, Germany). Artificial substrates were added to the homogenate at a final concentration of MUF-glucosidase ...
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bioRxiv - Cell Biology 2023Quote: The immunostaining procedure for microtubules consisted of: fixation for 7 min at 37°C with 4% paraformaldehyde (Sigma) in PBS ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.1% w/v Ferrozine (Disodium-4-[3-pyridin-2-yl-6-(4-sulfonatophenyl)-1,2,4-triazin-5-yl]benzosulfonate (Sigma-Aldrich) in dd ...
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bioRxiv - Cell Biology 2022Quote: ... Dead cells were excluded by 7-Amino-actinomycin (7-AAD, Sigma) staining and doublets were excluded by gating on FSC-H versus FSC-W and SSC- H versus SSC-W ...
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bioRxiv - Cell Biology 2023Quote: ... Dead cells were excluded with 7-aminoactinomycin D (7-AAD, Sigma). Cell were either sorted on AriaII or AriaIII cell sorter (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... except the protein sample was concentrated to 4 mg/ml and mixed with 0.005% 3-([3-Cholamidopropyl]dimethylammonio)-2-shydroxy-1-propanesulfonate (CHAPSO; Sigma Aldrich) immediately before vitrification ...
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bioRxiv - Microbiology 2020Quote: ... parahaemolyticus cells incubated in ASW microcosms at 4°C for 100 days were re-suspended in 50 mM potassium phosphate buffer (pH 7) containing 1 g of 3-mm-sized glass bead (Sigma), vortexed for 25 min ...
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bioRxiv - Bioengineering 2022Quote: ... All primary cells were used between subculture 3–7 and were routinely cryopreserved in 90% human serum (#H4522; Sigma-Aldrich) supplemented with 10% dimethyl sulfoxide (DMSO).
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bioRxiv - Biochemistry 2019Quote: The custom designed CompoZr® Zinc Finger Nuclease Plasmid targeted to exon 3 of the mouse ECH1 gene on chromosome 7 was obtained from Sigma. In-vitro transcription of the ZFN mRNA was performed following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... samples were resuspended in Urea 7 M and Thiourea 2 M buffer and desalted on Amicon Ultra-0.5 3 kDa centrifugal filters (Merck Millipore, Germany). Filters were filled to maximum capacity with buffers and centrifuged at 15,000 g for 10 min at 20°C ...
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bioRxiv - Immunology 2020Quote: Each peptide sample was desalted on Empore SPE C18 cartridges (standard density, bed inner diameter 7 mm, volume 3 ml; Sigma), then concentrated by vacuum centrifugation and reconstituted in 40 µl 0.1% (v/v ...
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bioRxiv - Plant Biology 2023Quote: ... The 30 collected fractions were combined into 15 pools and desalted on C18 Cartridges (Empore SPE Cartridges C18 standard density, bed I.D. 7 mm, volume 3 ml, Sigma-Aldrich). Each fraction was concentrated by vacuum centrifugation and reconstituted in 40 µl of 0.1% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged at a 1:3 to 1:6 ratio every 7 days using cell dissociation solution-non enzymatic (Sigma) and plated in fresh BME matrix droplets.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... SAG1.3 (3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide) was from Sigma. Cyclopamine-KAAD and SANT-1 ((4-Benzyl-piperazin-1-yl)-(3,5-dimethyl-1-phenyl-1H-pyrazol-4-ylmethylene)-amine ...
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bioRxiv - Biochemistry 2021Quote: ... and 4) the substrate solution (nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate, Sigma-Aldrich, St. Louis, MO). Other details were as described previously [Duk M. ...
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bioRxiv - Microbiology 2022Quote: ... Samples were centrifuged 1800 rpm for 6 minutes at 4°C and pellets were resuspended n 3 mL RBC lysis for 4 minutes (Sigma). Samples were centrifuged 1800 rpm for 6 minutes at 4°C and the pellets were resuspended in 200 μl of PBS + 2% FBS + 2 mM EDTA ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting adhered bacteria on the cover slip were stained with 200 μL of the membrane stain N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide (FM4-64) (Sigma) at a final concentration of 20 μg/mL for 5 minutes ...
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bioRxiv - Genetics 2020Quote: ... and the colour reaction was carried out with NBT/BCIP (4-nitroblue tetrazolium chloride/bromo-4-chloro-3-indolyl phosphate; Sigma). Sections were dehydrated and mounted with Vectamount (Vector Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... with PBS pH 7.4 for 10 min at 4400 x g and 4°C using an Amicon Ultra-4 concentrator with 3 kDa cutoff (Merck Millipore). The degree of labelling (DOL = 1.96 ...
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bioRxiv - Systems Biology 2024Quote: ... and kept at 4°C for a maximum of 4 hours until plasma was separated via centrifugation (2600g for 10 minutes at 4°C) (Sigma 3-16KL, Sigma Laboratory Centrifuges ...
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bioRxiv - Plant Biology 2024Quote: ... AGPs were finally detected using the following substrates: 4 mg of 5-bromo-4-chloro-3-indolylphosphate (BCiP; Sigma, USA) in 1 mL water and 9 mg of nitro-blue tetrazolium (NBT ...
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bioRxiv - Cell Biology 2022Quote: ... and 5-Bromo-4-chloro-3-indolyl phosphate-toluoidine were from Sigma-Aldrich, St ...
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bioRxiv - Biophysics 2020Quote: ... passaged every 3-4 days by trypsinization with trypsin-EDTA solution (Sigma-Aldrich) and moved to the corresponding Petri dishes ...
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bioRxiv - Plant Biology 2021Quote: Starting reagents 3-hydroxybenzaldehyde 4 and hydrazine hydrate were purchased from Sigma-Aldrich and phenylacetic acid 1 was purchased from Merck ...
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bioRxiv - Microbiology 2019Quote: ... for 5 min at 14,000 rpm at 4°C (Sigma 3-KIS centrifuge). Samples were snap-frozen in liquid N2 and delipidated by protein precipitation based on the protocol of Wessel and Flügge (61) ...
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bioRxiv - Cancer Biology 2021Quote: ... noggin and EGF and containing 10 µM nutlin-3 (Sigma #675576-98-4).
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bioRxiv - Neuroscience 2020Quote: ... The odorants used were 0.1% 3-octanol and 0.1% 4-methylcyclohexanol (Sigma Aldrich), controlled via three-way solenoids (Lee Company) ...
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bioRxiv - Immunology 2021Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (Sigma-Aldrich). Air-dried plates were read ...
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bioRxiv - Molecular Biology 2022Quote: ... 3×10-4 M monothioglycerol and 300 μg/ml human transferrin (Sigma Aldrich). Cells were plated in petri dishes (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... and blocked overnight at 4°C in DPBS with 3% BSA (Sigma, A9647) and 0.05% TritonX-100 ...
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bioRxiv - Bioengineering 2023Quote: ... For ECM conjugation using L-DOPA (3, 4- Dihydroxy-L-phenylalanine, D9628, SIGMA),[23] 2 mg ml-1 L-DOPA solution was prepared using 10 mM Tris-HCL buffer (pH balanced to 10.2 using 1 M NaOH ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were transferred to a 4% solution of (3-aminopropyl) triethoxysilane (Sigma #440140) in acetone for one hour ...
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bioRxiv - Microbiology 2023Quote: ... and used without further purification: 4-Hydroxy-3-methoxycinnamaldehyde (458-36-6, Sigma), 3-ethoxy-4-hydroxybenzaldehyde (121-32-4 ...
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bioRxiv - Molecular Biology 2020Quote: ... The 40 µL RNA sample destined for CMC-labeling was added to 400 µL BEU+CMC buffer (50 mM Bicine, pH 8.5; 4 mM EDTA; 7 M urea; 200 mM CMC (Sigma)) ...
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bioRxiv - Molecular Biology 2020Quote: ... The reagents 7-ethoxycoumarin (CAS: 31005-02-4, purity: 99.7 %) and RNAlater®was purchased from Sigma-Aldrich (Germany), TRIzolTM reagent from ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Fifteen µg of cell lysate from each hESC line was incubated in 150 µl acetate buffer (pH 4.0) containing a final concentration of 62.5 µM Ala-Ala-Phe-7-amido-4-methylcoumarin (AAF-AMC) (Sigma #A3401) for 20 h at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: TPP1 enzyme activity was measured fluorometrically based on fluorescence of the chromophore 7-amino-4-methylcoumarin (AMC) (Sigma #A9891) as described previously (Lojewski et al. ...
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bioRxiv - Immunology 2023Quote: ... Mice were sacrificed at day 7 (X31) and day 4 (RSV) post infection (p.i.) and lung lobes were stored in RNA-later (Sigma). Lung tissue was homogenized using a TissueLyser LT (Qiagen) ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were then stained with 7-Aminoactinomycin D (7-AAD, Sigma-Aldrich) and an AF488-conjugated anti-KI-67 antibody (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... CM metabolite 7-hydroxycoumarin (7-HC; Cat. No. H24003, Sigma-Aldrich Corp.), BFC ...
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bioRxiv - Neuroscience 2020Quote: ... the eluate was washed 3 times with PBS in an Amicon Ultra-4 Centrifugal Filter Unit (NMWL 3 kDa, Millipore, UFC800324) and finally concentrated to < 250 µl ...
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bioRxiv - Biochemistry 2022Quote: ... Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 150 mg/L sodium 4-methyl-2-oxovalerate (Sigma K0629) ...
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bioRxiv - Biochemistry 2022Quote: ... Dimethyl LV labeling for geminal pair determination used 150 mg/L 2-Keto-(3-methyl-13C)-butyric-4-13C,3-d acid sodium salt (Sigma 589063). Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887 ...
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bioRxiv - Biochemistry 2022Quote: ... 13C-ILV labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 70 mg/L 2-Ketobutryic acid-4-13C,3,3,d2 sodium salt hydrate (Sigma 589276 ...
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bioRxiv - Biochemistry 2023Quote: ... DHEA sulfate (5-androsten-3β-ol-17-one-3-sulfate) and testosterone (4-androsten-17β-ol-3-one) were purchased from Sigma Aldrich. 11β-hydroxyandrostenedione (11β-hydroxy-4-androstene-3,17-dione) ...
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bioRxiv - Neuroscience 2020Quote: ... 7 MgCl2 (Sigma-Aldrich), 0.5 CaCl2 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... pH=7 (Sigma, USA). After 10 hr ...