Labshake search
Citations for Millipore Sigma :
251 - 300 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... Membranes were blocked with 5% milk in 1X PBS + 0.05% Tween-20 (Sigma) for 1 hour and then incubated overnight at 4°C with primary antibodies ...
-
bioRxiv - Neuroscience 2022Quote: ... permeabilized in ice-cold acetone (−20°C, 5 min, Sigma-Aldrich, Cat# 179124), then blocked in PBS with 2% goat serum (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were washed 5 times in TBS + 0.1% Tween 20 (Sigma Aldrich, P1379) before being incubated for 1 h at room temperature with secondary antibodies HRP conjugated (Bethyl ...
-
bioRxiv - Cancer Biology 2023Quote: ... in PIPES solution (120 mM NaCl, 5 mM KCl, 20 mM PIPES (Sigma), 0.45% glucose ...
-
bioRxiv - Cancer Biology 2023Quote: ... fitted with a guard column (20 mm × 2.1 mm i.d. 5 μm, Millipore) for the liquid chromatography separation ...
-
bioRxiv - Cancer Biology 2021Quote: ... prepared as previously described [25], or vehicle control (65% PEG-400, 20% Tween-20, 10% H2O, 5% DMSO (all Sigma Aldrich)) was initiated when tumours became palpable (approximately 10 days after inoculation of Eµ-Myc cells ...
-
bioRxiv - Neuroscience 2020Quote: ... 1X penicillin/streptomycin and anti-mitotic reagents (20 μM 5-fluoro-2-deoxyuridine and 20 µM uridine, both from Sigma-Aldrich). The medium replacement removed the electroporation buffer and dead cells.
-
bioRxiv - Evolutionary Biology 2019Quote: ... Lysate was heat-treated at 70 °C for 5 minutes to denature proteins after which one 20 μl aliquot was mixed with 20 μl Triglyceride Reagent (Sigma T2449) and one 20 μl aliquot was mixed with 20 μl of PBS and incubated at 37 °C for 30 minutes ...
-
bioRxiv - Neuroscience 2019Quote: ... Dissociated DRG neurons were plated onto plastic coverslips in 24-well plates after washing and grown in culture medium (MEM in 5% fetal bovine serum, 20 μM 5-fluoro-2-deoxyuridine, 20 μM uridine, 100 units /ml penicillin and 100 ng/ml streptomycin, Sigma Aldrich) for 3 days at 37°C in a CO2-humidified incubator ...
-
bioRxiv - Cancer Biology 2020Quote: ... using a 20 min linear gradient from 5% B to 35% B (A: 20 mM ammonium formate (cat No. 70221, Sigma, USA) in water ...
-
bioRxiv - Biochemistry 2023Quote: ... Cell toxicity was measured in 96-well plates (∼5×104 cells/well) using a lactate dehydrogenase (LDH) cytotoxicity kit (Sigma-Aldrich, Cat # 11644793001). Absorbance was measured at λ=490 nm ...
-
bioRxiv - Physiology 2023Quote: ... were transferred to OP50-seeded plates or RNAi-seeded NGM plates supplemented with 50 μM of 5-fluoro-2-deoxyuridine (FUDR, Sigma) to prevent progeny growth ...
-
bioRxiv - Biophysics 2021Quote: ... Microcrystals 1-5 × 5-10 × 10-20 μm3 in size were passed through 100 micron plastic mesh filters (Millipore, USA) in the same mother liquor composition to eliminate the large single crystals and other impurities before the data collection ...
-
bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked in 5% BSA TBS-T (5% BSA in 1x Tris Buffered Saline, 0.1% Tween 20, Sigma Aldrich) for 1 hour and incubated with primary antibodies overnight in 5% BSA TBS-T (MMP1 1:1000 ab137332 ...
-
bioRxiv - Biophysics 2020Quote: ... Microcrystals 1-5 × 5-10 × 10-20 μm3 in size were passed through 100 micron plastic mesh filters (Millipore, USA) in the same mother liquor composition to eliminate the large single crystals and other impurities before the data collection ...
-
bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in blocking buffer (5% non-fat milk or 5% FBS diluted in TBS-T buffer (0.05% Tween-20 (Sigma-Aldrich) in tris-buffered saline (TBS) ...
-
bioRxiv - Physiology 2021Quote: ... Membranes were blocked on a 5% dry nonfat milk solution or 5% BSA with Tris-buffered saline (TBS) with 0.1% TWEEN® 20 (Sigma) at room temperature for 1 hour ...
-
bioRxiv - Neuroscience 2023Quote: mice received an intraplantar injection of 20 μl of 5% formalin diluted from formaldehyde solution (5% v/v from 37% stock formaldehyde solution (Sigma)) in sterile saline ...
-
bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked using 5% BSA or 5% non-fat dried milk in TBS containing 0.05% Tween 20 (Sigma-Aldrich) for 1h at 15-25°C ...
-
bioRxiv - Biophysics 2023Quote: ... dissolved in Tris buffer (5 mM TRIS, 5 mM NaCl, 20 mM MgCl2, pH 7.5 (all chemicals from Sigma-Aldrich)
-
bioRxiv - Cancer Biology 2024Quote: ... 10 nM siRNA against DDX3X (5’-CCUAGACCUGAACUCUUCAGAUAAU dTdT-3’) or 20 nM siRNA against GRSF1 (5’-GUGCCUCUCUGCUGCCGCAdTdT-3’) which were synthesized by Sigma. Cells were subsequently incubated at 37 °C for 48 h before harvesting and analysis ...
-
bioRxiv - Neuroscience 2020Quote: ... using the 20S Proteasome Activity Assay Kit (Millipore, APT280) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: Serum IL-6 levels were quantified using a commercial mouse IL-6 ELISA kit (RAB0308, Sigma) according to the manufacturer’s instructions and analyzed using spectra-iMAX (Molecular Devices).
-
bioRxiv - Immunology 2022Quote: Serum insulin was determined using a rat/mouse insulin ELISA kit (Millipore, Billerica, Merck KGaA, USA) as instructed ...
-
bioRxiv - Genetics 2021Quote: ... Mouse plasma insulin and leptin levels were determined by using Rat/ Mouse Insulin ELISA Kit (Millipore, EZRMI-13K for insulin and EZML-82K for leptin ...
-
bioRxiv - Microbiology 2021Quote: ... the antibodies isotypes were determined by ELISA using a mouse Ig isotyping kit (ISO-2, SIGMA).
-
bioRxiv - Cell Biology 2021Quote: ... cells were lysed in 125 μl of lysis buffer provided with a CAT ELISA kit (Sigma). CAT expression and Renilla luciferase activities were measured and the ratio was calculated.
-
bioRxiv - Physiology 2020Quote: ... Insulin release from islets was measured as described with Rat/Mouse Insulin ELISA kit (Millipore Sigma) (26).
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were routinely subjected to mycoplasma testing using the Mycoplasma PCR ELISA kit (Sigma Aldrich, 11663925910) and tested negative.
-
bioRxiv - Developmental Biology 2020Quote: ... 20 day-one adult animals were transferred onto NGM plates containing 1 mM aldicarb (Sigma) without food ...
-
bioRxiv - Microbiology 2020Quote: ... Immediately prior to plating on agar plates containing 20 mM 4-chloro-phenylalanine (Sigma-Aldrich), the cells were washed twice with phosphate-buffered saline (PBS ...
-
bioRxiv - Immunology 2021Quote: ... The plates were then washed 3 times with PBS containing 0.05% Tween-20 (Sigma-Aldrich), blocked with 150 mL ELISA/ELISPOT Diluent (Life Technologies Corporation ...
-
bioRxiv - Cancer Biology 2019Quote: ... Each plate was transfected with 20 μg of overexpression plasmid with 100 μg PEI (Sigma). After 48 h cells were collected in TBS ...
-
bioRxiv - Microbiology 2020Quote: ... Plates were subsequently blocked with Blocker Casein 1% supplemented with 0.05% Tween 20 (Sigma-Aldrich) for 1 h at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... Plates were washed 6 times with washing buffer (1xPBS with 0.05% Tween 20 (Sigma-Aldrich)) and incubated with 170 µL blocking buffer per well (1xPBS with 2% BSA and 0.05% Tween20 (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... A 96-well plate was treated with 20 μg/ml poly-D-Lysine (Sigma-Aldrich) for 1h at room temperature and washed twice with 1 x DPBS (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2020Quote: ... Plates were washed 6 times with washing buffer (1xPBS with 0.05% Tween 20 (Sigma-Aldrich)) and incubated with 170 μL per well blocking buffer (1xPBS with 2% BSA and 0.05% Tween20 (Sigma) ...
-
bioRxiv - Microbiology 2022Quote: Congo red indicator agar plates were prepared by adding 20 mg/ml Congo red (Sigma) and 10 mg/ml Coomassie brilliant blue G (Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... Plates were coated with 20 μg/ml laminin (Sigma, L2020 or Trevigen, 3446-005-01) for all lines except PBT-04 ...
-
bioRxiv - Cell Biology 2020Quote: ... Triglyceride Assay kit (Sigma, TG-5-RB), and Glucose (GO ...
-
bioRxiv - Immunology 2021Quote: ... The plates were washed (5 times with PBS-T) and AP substrate (Sigma) was added ...
-
bioRxiv - Cell Biology 2021Quote: ... plates were incubated in 5% (v/v) Hellmanex III solution (Z805939-1EA; Sigma) overnight ...
-
bioRxiv - Immunology 2021Quote: ... The plates were washed (5 times with PBS-T) and AP substrate (Sigma) was added ...
-
bioRxiv - Microbiology 2020Quote: ... Adjusted cultures were 1:10 serially diluted 5-times and 5 µl of each dilution spotted on YPD agar plates containing indicated concentrations of caspofungin (Sigma). Plates were incubated at 30°C for 3 days and photographed ...
-
bioRxiv - Microbiology 2022Quote: ... A 2.5- or 5-μl volume of ice-cold extracts were spotted along the bottom of polyethyleneimine-cellulose TLC plates (Millipore). The spots were air dried ...
-
bioRxiv - Neuroscience 2020Quote: ... the iN cells were dissociated with Accutase and replated with mouse glia cells on Matrigel-coated coverslips in 24-well plate (150k cells/well) in Neurobasal 1% serum medium containing 5 μM 5-fluorodexoyuridine (FUDR, Sigma) and 4 μM Uridine (Sigma ...
-
bioRxiv - Genomics 2023Quote: ... per 632.5 uL 2xTD buffer (20 mM Tris-HCl pH 7.6, 10 mM MgCl2, 20% v/v Dimethyl Formamide (Sigma-Aldrich, 227056-100ML)) was added and mixed by pipetting 10 x ...
-
bioRxiv - Neuroscience 2023Quote: ... per 632.5 uL 2xTD buffer (20 mM Tris-HCl pH 7.6, 10 mM MgCl2, 20% v/v Dimethyl Formamide (Sigma-Aldrich, 227056-100ML)) ...
-
bioRxiv - Cell Biology 2019Quote: ... 5 μL of the serial dilutions were transferred from the 96-well plate onto YPD agar plates with or without additional 5 mM guanidinium hydrochloride (Sigma-Aldrich) and/or 50 ng/mL cycloheximide (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2020Quote: ... Mice were subjected to cutaneous immune challenge by applying 20 µl of 3-5% 4- Ethoxymethylene-2-phenyl-2-oxazolin-5-one (Sigma-Aldrich) in 1:2 acetone:olive oil ...