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Citations for Millipore Sigma :
4901 - 4950 of 10000+ citations for Recombinant Human ETFB Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... anti-PAP 1:1000 (for detecting TAP-tagged proteins) (Sigma-Aldrich, P1291), anti-Rad53 1:2000 (abcam ab104232) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was quantified using a Direct Detect Spectrometer (Millipore, DDHW00010- WW), and 40 μg of proteins/sample was loaded onto S-trap columns (Protifi ...
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bioRxiv - Microbiology 2022Quote: The MLB1 CPNTD protein was produced in Rosetta 2 (DE3) cells (Novagen) cultured in 2×YT media with overnight expression at 18 °C induced with 0.4 mM IPTG ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti-Prosurfactant Protein C (pro-SP-C) antibody (1:4000) (Millipore, #AB3786). Antibodies were incubated in 2% powder milk in TBS pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated using SDS-PAGE and transferred to PVDF membranes (Millipore) for Western blot analysis ...
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bioRxiv - Plant Biology 2022Quote: ... incubated 40 min with 50 µL of magnetic protein G Dynabeads (Millipore) fused to a GFP monoclonal antibody (1/500 in PBS 0.002 % tween) ...
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bioRxiv - Cancer Biology 2022Quote: ... and proteins were transferred onto a PVDF membrane (Millipore, Cat. No. ISEQ00010). The membrane was blocked with 5% non-fat milk in tris buffered saline with 0.1% Tween 20 (TBS-T ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted protein was concentrated with a 30 kDa centrifugal filter unit (Millipore) and purified by size-exclusion chromatography (HiLoad 16/600 Superdex 200 ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted proteins were concentrated using an Amicon Ultra centrifugal device (Millipore) with 100 kDa MW cut-off ...
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bioRxiv - Biochemistry 2022Quote: ... Solubilized protein was loaded onto 1.5 mL of M2 affinity matrix (Sigma) equilibrated with wash buffer (20 mM MES pH 6.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrated protein was immobilized on cyanogen bromide activated Sepharose 4G (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... the reduced proteins were alkylated with 150 mM Iodoacetamide (Millipore, Bedford, USA) in darkness at room temperature for 30 min ...
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bioRxiv - Biochemistry 2022Quote: All proteins were reduced by incubating with 10 mM of dithiothreitol (Sigma) at 56 °C and alkylated by 27.5 mM of iodoacetamide (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... DNA-protein complexes were crosslinked using 1% final concentration of formaldehyde (Sigma) for 10min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then transferred to 0.2 μm nitrocellulose membranes (Millipore, 10600001) using a wet tank system at 115 V for 1 h at 4°C in Tris-Glycine buffer (25 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... Commercially available flavivirus E protein-specific mAb 4G2 (EMD Millipore, Darmstadt, Germany), rabbit anti-ZIKV prM and C antibodies (GeneTex ...
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bioRxiv - Genetics 2022Quote: ... and 0.1% sodium dodecyl sulfate) containing a protein inhibitor cocktail (Sigma-Aldrich). The homogenate was centrifuged for 10 minutes at 10,000g and the supernatant was collected ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentrations were determined using a Direct Detect Infrared Spectrometer (Millipore Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... we used the protein synthesis inhibitor cycloheximide (CHX, Sigma-Aldrich, Deisenhofen, Germany). CHX inhibits eEF2-mediated translocation in eukaryotic ribosomes (Schneider-Poetsch et al. ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-microtubule-associated protein 2a+b (MAP2, Sigma #M1406; 1:2000), rabbit anti-tyrosine hydroxylase (TH ...
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bioRxiv - Plant Biology 2022Quote: ... and fusion proteins purified according to manufacturer’s instructions (GE Healthcare or Novagen). Protein-protein binding buffer contained ...
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bioRxiv - Neuroscience 2022Quote: ... RRID:AB_839504) and myelin basic protein (MBP; rat anti-MBP; #MAB386, Millipore; RRID:AB_94975).
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bioRxiv - Neuroscience 2022Quote: Total protein concentrations were measured using the Pierce BCA method (Sigma, USA). Equivalent amounts of protein (40 mg per lane ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated using SDS-PAGE and transferred to PVDF membrane (Millipore). Immunoblotting was achieved using the indicated antibodies listed in the supplemental table 3 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein bands were revealed with enhanced chemiluminescence reagent (211595, Millipore, Immobilon Crescendo) and images captured with a KwikQuant Imager (Kindle Biosciences) ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-Myelin Basic Protein antibody [12] (MBP) (Millipore; rat monoclonal, 1:500), Mouse anti-A2B5 (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... Oxygen consumption rates were calculated and normalized for protein content by Novagen BSA assay (71285-3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Eluted proteins were desalted using either Amicon Ultra centrifugation filters (Merc Millipore) or Zeba Spin desalting columns (Thermo Fisher scientific ...
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bioRxiv - Immunology 2022Quote: ... samples were incubated in the presence of Protein A Sepharose beads (Millipore) for 1-2h ...
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bioRxiv - Cell Biology 2024Quote: ... Protein bands were detected by Luminol-based enhanced chemiluminescence (EMD Millipore WBKLS0500). Immunoblots were captured on film and quantified using Image J.
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bioRxiv - Neuroscience 2022Quote: ... Proteins were visualized using chemiluminescence (Immobilon Western Chemiluminescent HRP Substrate, Millipore, #WBKLS0500) on Bio-Rad ChemiDoc (XRS+) ...
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bioRxiv - Neuroscience 2022Quote: ... and the protein concentration determined with a BCA assay (Sigma, Deisenhofen, Germany). Equal amounts of protein samples were separated on 8% Tris/glycine gels and then transferred to nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... Transferring the protein from the gel to the PVDF membrane (Millipore #ISEQ00010). Membranes were blocked with 5% milk for one hour at room temperature and incubated with appropriate dilutions of primary antibody overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... in the presence or absence of 0.5 mg/mL OVA protein (Sigma) in cRPMI at 37℃ in 5% CO2 incubator for 72 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected with anti-Flag antibody (Sigma, F1804, monoclonal, 1:1,000) or anti-Sso antibody (rabbit antiserum ...
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bioRxiv - Cell Biology 2022Quote: ... The protein complex was concentrated using centrifuge filters (Amicon Ultracel-30K, Millipore) and subjected to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg ...
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bioRxiv - Physiology 2022Quote: ... Protein extraction was carried out on ice using RIPA buffer (Sigma Aldrich) supplemented with protease inhibitor cocktail (Sigma) ...
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bioRxiv - Bioengineering 2022Quote: ... and then filtered through a 0.45-μm low protein binding membrane (Millipore). HEK293T cells were seeded into 12-well plates at approximately 40-50% confluency and 50 μl filtered virus-containing supernatant was added to the 12-well plates ...
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bioRxiv - Neuroscience 2022Quote: ... the expression of GST fusion proteins was induced with isopropylthiogalactoside (IPTG, Sigma) at 16 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... Protein extracts were immunoprecipitated with a PP2A C-specific antibody (1D6; Millipore). An appropriate phosphorylated peptide (amino acid sequence K-R-pT-I-R-R ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were then transferred to 3 kDa protein-filter columns (Sigma-Aldrich) and centrifuged at 12,000g for 45 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Optimal protein expression and solubility was achieved in RosettaTM(DE3) pLysS (Novagen) for wild-type His::PfPKG ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended cells were supplemented with Bug Buster protein reagent (Millipore #70584-3) before being sonicated ...
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bioRxiv - Biochemistry 2024Quote: ... immunoreactive proteins were visualized using Immobilon® Classico Western HRP Substrate (Millipore) or Immobilon® Forte Western HRP Substrate (Millipore ...
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bioRxiv - Bioengineering 2024Quote: ... One hundred µL of protein samples or lysostaphin standards (SIGMA-L9043-5MG) diluted in 0.01% Tween 80 (SIGMA-P5188-100ML ...
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bioRxiv - Cancer Biology 2024Quote: ... equal amounts of protein were run and transferred onto nitrocellulose membranes (Millipore). The membranes were blocked with 5 % milk and incubated with the following antibodies ...
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bioRxiv - Molecular Biology 2024Quote: ... Separated proteins were transferred to Immobilon FL polyvinylidene difluoride (PVDF) membrane (Millipore) for immunoblotting ...
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bioRxiv - Microbiology 2024Quote: ... RNA and proteins were extracted from the fractions using Trizol reagent (Sigma) as per manufacturer’s instruction and the RNA from the polysomal fractions (≥3 associated ribosomes ...
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bioRxiv - Bioengineering 2023Quote: ... The proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Millipore, Cat# ISEQ00010), and blocked with blocking buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... DNA/protein complexes were then precipitated with anti-CTCF antibody (EMD Millipore) or rabbit IgG conjugated with protein A and G beads ...