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Citations for Millipore Sigma :
4801 - 4850 of 7667 citations for Recombinant Ovine IL4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The protein concentrations of the samples were determined by the BCA assay kit (Sigma-Aldrich, USA). Digitonin (Calbiochem ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were denatured by addition of 2X Laemmeli buffer/10% β-mercaptoethanol (Bio-Rad/Sigma, respectively) to samples and subjection of samples to boiling for 2 min at 95°C ...
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bioRxiv - Microbiology 2022Quote: ... 400 μg of total protein lysates were incubated overnight with Anti-FLAG M2 Magnetic Beads (Sigma) or control G-coupled magnetic beads ...
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bioRxiv - Molecular Biology 2022Quote: ... These protein samples were separated using 10% SDS–PAGE and transferred to nitrocellulose membranes (Millipore, USA). Then ...
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bioRxiv - Microbiology 2022Quote: ... 75 μg of protein was precipitated with an equal volume of 20% trichloroacetic acid (TCA, Sigma) for 16 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... to the hen’s egg protein ovalbumin (OVA) (20 mg/0.5 mL PBS; grade V; Sigma-Aldrich) using cholera toxin (CT ...
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bioRxiv - Plant Biology 2022Quote: ... Total protein loading was visualized by staining nitrocellulose membranes with Ponceau S solution (Sigma-Aldrich, P7170).
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bioRxiv - Plant Biology 2022Quote: ... The separated proteins were transferred to polyvinylidene difluoride membranes (Immobilon-P; Merck Millipore, Burlington, MA, USA) and analyzed with the following antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... Serum proteins were first precipitated by addition of 10% (w/v) trichloroacetic acid (TCA; Sigma, 49010) and incubated at -20°C for up to 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were then transferred (300 mA, 3h) onto ethanol-activated Immobilon-P PVDF membrane (Millipore) before blocking with 3% Bovine Serum Albumin in Tris-buffered saline-Tween (TBST ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were transferred to Immobilon-P polyvinylidene difluoride (PVDF) membranes (Millipore Sigma, Burlington, MA, USA) using a semidry blotting system (Nihon Eido ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were then transferred to a methanol (MeOH)-activated polyvinylidene fluoride (PVDF) membrane (Immobilon-P; Millipore) using a wet tank electro-blotting apparatus (Mini Trans-Blot ...
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bioRxiv - Plant Biology 2023Quote: Nuclear proteins were extracted from frozen poplar stem using the CellLytic™ PN kit (Sigma, USA) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... and then intracellularly stained with Peridinin chlorophyll protein-Cy5.5-conjugated anti-mouse TNF-α (Millipore, Billerica) and phycoerythrin-conjugated anti-mouse IL-4 (eBioscience ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were concentrated by using the Amicon Ultra centrifugal device (NMWL: 3K) (Merck, Millipore Cat# UFC500324), and the concentration was determined by the Pierce BCA protein assay kit (Thermo Fisher Scientific Cat# 23225) ...
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bioRxiv - Physiology 2022Quote: ... Hyaluronic acid/hyaluronan (HA) was stained overnight using biotinylated HA binding protein (Millipore, Burlington, MA; 385911). All sections were washed and incubated with VECTASTAIN® ABC-HRP kit (Vector laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... Protein reduction and alkylation of cysteine was performed with 10 mM TCEP (Millipore Sigma; Cat#: C4706) and 40 mM CAA (Millipore Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 167 mM NaCl) and pre-cleared with 75 µL of Protein A agarose (50% slurry, Sigma). Pre-cleared chromatin lysate was immunoprecipitated by a ChIP-grade rabbit polyclonal antibody against histone H3K9me3 (Cat ...
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bioRxiv - Plant Biology 2022Quote: ... Captured proteins were eluted off FLAG beads using 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich). Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were diluted 1:3 with Urea-buffer (4.5 mM Urea (Sigma-Aldrich Cat#U1250), 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... The same membrane was stripped of antibody and total protein was determined with Coomassie (Sigma, B0149) staining ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by a 4 h of incubation with protein A/G agarose beads (LOT: 3460992, Millipore). The purified DNA fragments bound by ATF3 were analyzed using qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... RNA-protein complexes were retrieved from the cleared lysate on anti-Flag M2 beads (Sigma Aldrich) and eluted using 3x Flag peptide ...
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bioRxiv - Molecular Biology 2022Quote: ... The purified protein was concentrated using Amicon Ultra centrifugal filters with 10 kDa cut-off (Millipore) and dialyzed overnight against storage buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2022Quote: ... Separated proteins were transferred from the gel to a polyvinylidene fluoride membrane (PVDF, Immobilon-P Millipore) using a wet blot system (Mini-PROTEAN Tetra Cell ...
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bioRxiv - Plant Biology 2022Quote: ... bound proteins were eluted using buffer A containing 500ng/μL 3X FLAG® peptide (Sigma; F4799) for followed SDS-PAGE.
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bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
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bioRxiv - Biophysics 2022Quote: ... the protein was refolded via 10-fold dilution in either 5 mM oxidized glutathione (Millipore 3542) or 3.3 mM oxidized glutathione + 1.7 mM reduced glutathione dissolved in PBS buffer ...
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bioRxiv - Cell Biology 2022Quote: Detection of an interaction between proteins was assessed using the Duolink II Detection Kit (Sigma-Aldrich) according to the manufacturer specifications ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 30µg protein was loaded on 10% SDS-PAGE and then transferred to PVDF membrane (Millipore) followed by blocking ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Proteins were transferred onto nitrocellulose (chemiluminescence imaging) or to Immobilion FL PVDF membranes (Millipore, fluorescence imaging) using transfer buffer with 10% ethanol ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound proteins were eluted with 150 μg/mL 3×Flag (Sigma-Aldrich, Cat# F4799) at 4 °C for 1.5 h ...
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bioRxiv - Biochemistry 2024Quote: ... The affinity captured proteins were then digested with 4 μg of MS grade trypsin (Sigma- Aldrich) in 500 μL of digestion buffer [2 M urea ...
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bioRxiv - Biophysics 2023Quote: ... The retrieved protein was concentrated to 0.5 ml with Amicon Ultra (50 kDa cutoff, EMD Millipore) and followed by size-exclusion chromatography (SEC ...
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bioRxiv - Cancer Biology 2024Quote: The interaction between NINJ1 and xCT proteins was assessed using the Duolink Proximity Ligation Assay (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: RIP assays were performed using the Magna RIPTM RNA-binding protein immunoprecipitation kit (Millipore, Massachusetts, USA). Cells in 15 cm dishes were lysed in complete RIP lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein expression was induced by 1 mM isopropyl 1-thio-β-D-galactopyranoside (IPTG, Sigma) at 16°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... expression of fusion proteins was induced for 24h by the addition of doxycycline (Sigma-Aldrich # D9891) to 5 µg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... Eluted immunoprecipitated protein was precipitated by adding TCA and Tween 20 (Sigma Aldrich, St. Louis, USA) to a final concentration of 10% and 0.5% respectively and incubated on ice for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Forty micrograms of protein samples were denatured in reducing loading buffer containing β-mercaptoethanol (M3148, Sigma) and 0.0002% bromophenol blue at 95°C for 5 minutes and separated in pre-casted 12% or 7.5% Tris-Glycine polyacrylamide gel using the Criterion cell system (Biorad) ...
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bioRxiv - Neuroscience 2024Quote: Immunostaining of proteins was conducted on fixed cells using 4% (wt/vol) paraformaldehyde (252549, Sigma-Aldrich) as previously described [61] ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The purified protein was concentrated to 0.6 mg/ml using an Amicon centrifugal contentrator (EMD Millipore), flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto methanol pre-activated polyvinylidene fluoride (PVDF) membranes (IPFL00010, Immobilon-P; Merck-Millipore). Membranes transferred from TGX Stain-Free™ gels were imaged and quantified for posterior normalization with a ChemiDoc XRS+ (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies to human proteins used in this study included: Actin (1:20,000 dilution (MAB1501, Millipore, RRID:AB_2223041)) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were further separated on the 12% SDS-PAGE and transferred on the PVDF membrane (Millipore) using semi dry trans-blot (Bio-Rad) ...
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bioRxiv - Developmental Biology 2024Quote: ... The proteins were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore, Temecula, CA) and then blocked in 5% non-fat milk ...
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bioRxiv - Genomics 2024Quote: ... the system was calibrated using the Protein Standard Mix 15-600 kDa (Sigma-Aldrich, Darmstadt, Germany). Calibration was done under a flow rate of 0.5 mL min-1 with a mobile phase containing 50 mM Tris-HCl buffer (pH 7.5) ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were eluted in buffer 5 [buffer 3 plus 200 μM FLAG peptide (Sigma-Aldrich)] for 1.25 hours ...
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Treatment with furosemide indirectly increases inhibitory transmission in the developing hippocampusbioRxiv - Neuroscience 2023Quote: ... The proteins were then transferred (300 mA, 3h) onto ethanol-activated Immobilon-P PVDF membrane (Millipore) before blocking with 3% Bovine Serum Albumin in Tris-buffered saline-Tween (TBST ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...