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Citations for Millipore Sigma :
4751 - 4800 of 10000+ citations for Recombinant Human CYB5A Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... The target proteins were collected and desalted using Amicon (10kDa cut) (Millipore) before subjecting to the HiTrap Q column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted by competition using the FLAG peptide (Sigma).
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bioRxiv - Microbiology 2020Quote: ... Target proteins were visualized with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore).
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred onto a polyvinylidene difluoride membrane (#IPVH00010; Millipore, Bedford, MA). The membrane was blocked and probed with primary and secondary antibodies sequentially ...
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bioRxiv - Microbiology 2020Quote: ... GST bound protein was then cleaved using biotinylated thrombin (Merck Millipore; 69672) according to the manufacturers instructions overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and the resolved proteins were transferred onto PVDF membranes (Immobilon-P, Millipore) using a semi-dry blotting assembly (Amersham Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... Total protein extracts supplemented with β-mercaptoethanol (750 mM final, Sigma-Aldrich) were analysed by western blot ...
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bioRxiv - Cell Biology 2021Quote: The Magna RNA-binding protein immunoprecipitation kit (Merck Millipore, Billerica, MA, USA) was applied for the assay ...
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bioRxiv - Cell Biology 2020Quote: ... Then pre-processed protein A agarose (Catalog No. P3476, Sigma, MO, USA) was added to mixture ...
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bioRxiv - Biophysics 2021Quote: ... Protein expression was performed in Origami™ 2 (DE3) competent cells (Novagen) using LB medium ...
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bioRxiv - Biophysics 2021Quote: ... Crosslinked proteins were precipitated with 20% (w/v) trichloroacetic acid (TCA, Sigma) on ice for 90 minutes ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were visualized by gel staining with Coomassie Brilliant Blue (Sigma Aldrich).
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bioRxiv - Cancer Biology 2021Quote: ... Antibody-reactive proteins were detected by western chemiluminescent HRP substrate (ECL) (Millipore). Images were acquired by FluorChem FC3 image analyzer (Molecular Devices).
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked with 5% skim milk in TBST (10 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was filtered with a 0.22 μm pore size filter (Millipore) prior to size-exclusion chromatography (SEC ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were probed with anti-thymidylate synthase antibody (Millipore, Cat:MAB4130, 1:500), anti-CDK6 antibody (Cell Signalling ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were detected with Luminata Forte Western HRP Substrate (EMD Millipore) or SuperSignal™ West Atto Ultimate Sensitivity Substrate (A38555 ...
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bioRxiv - Cell Biology 2022Quote: ... and then the proteins were transferred onto polyvinylidene difluoride membranes (Millipore, IPVH00010). Membranes were then probed with anti-α-SMA (Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... and it was determined by BCA protein assay (Sigma, Sao Paulo, Brazil). A total of 10 µg protein concentrate was used in a 10% or 12% polyacrylamide gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated with ultra-filter 0.5 (Millipore, Sao Paulo, Brazil), and it was determined by BCA protein assay (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... protein expression was induced using 1 μg/mL of doxycycline (Sigma, D9891) for 16 h.
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bioRxiv - Biochemistry 2022Quote: ... 50µL of a suspension of Protein G coupled to agarose beads (Sigma) previously equilibrated in Bax buffer (20mM Tris/HCl pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... 500μg of protein was incubated on magnetic beads (FLAG M2 beads (Sigma), GFP beads (Chromotek ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were transferred to Immobilon-P PVDF membrane (Merck Millipore, MA, U.S.A). The membranes were incubated for 1h using 5% low-fat milk solution in 1X TBS (50 mM Tris-Cl ...
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bioRxiv - Molecular Biology 2022Quote: ... Total protein was assayed by incubation with Ponceau staining (Sigma-Aldrich P7170), which was later destained and imaged on a Sapphire biomolecular imager (Azure Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins entrapped in gel bands were reduced with 10 mM dithiothreitol (Sigma) at 60°C for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... The proteins were wet-transferred to a PVDF membrane (Immobilon-P; Millipore) for 2.5 hours at 100 V ...
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bioRxiv - Neuroscience 2022Quote: ... The final measurements were normalized to protein content using sulforhodamine B (Sigma) (Johnson et al. ...
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bioRxiv - Bioengineering 2022Quote: Biotinylated proteins were obtained using Biotin N-hydroxysuccinimide ester reagent (Sigma Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... and associated proteins were eluted in 125 μg/ml 3xFLAG peptide (Sigma) for 30 minutes on ice.
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bioRxiv - Biophysics 2022Quote: ... and ASH2L proteins were individually expressed in Escherichia coli (Rosetta II, Novagen) and purified as described previously (66) ...
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bioRxiv - Biochemistry 2022Quote: ... His6-tagged proteins were purified with Ni-NTA His-Bind Superflow (Novagen) and dialyzed against PBS using a Pur-A-Lyzer™ Midi Dialysis Kit (Merck).
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bioRxiv - Cancer Biology 2022Quote: ... Protein G Sepharose 4 Fast Flow Beads (Millipore Sigma, GE17-0618-01) were added and incubated for 1 h at 4°C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved by SDS-PAGE and transferred to PVDF membrane (Millipore). Membranes were incubated overnight with primary antibodies (Antibody list shown in Table 3) ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were then concentrated by ultrafiltration using Amicon filters (EMD Millipore).
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bioRxiv - Neuroscience 2019Quote: ... Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma). Immunoprecipitates were incubated for at least 2 h at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 300 μg proteins were incubated with 20 mM dithiothreitol (DTT; Sigma Aldrich) for 10 min at 95°C on a thermomixer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred onto 0.45 µm PVDF Immobilon-FL membranes (Millipore Sigma) and visualized using the ChemiDoc XRS+ (Biorad ...
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bioRxiv - Neuroscience 2020Quote: ... Protein lysates were concentrated using Microcon-10kDa Centrifugal Filter Units (Merck Millipore), according to manufacturer’s instructions and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... and immunoprecipitated using 50 μl of protein G agarose bead slurry (Millipore). Monoclonal antibodies recognizing CD2v-HA were generated by immunizing BALB/c mice with immunoprecipitated CD2v-HA (100 μg/dose) ...
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bioRxiv - Genetics 2021Quote: ... Transfer was confirmed by visualising protein bands with 0.1% Ponceau S (Sigma) in 5% acetic acid (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were transferred to Immobilon-P Polyvinylidene fluoride (PVDF) membranes (Millipore) in Tris-Glycine-SDS buffer containing 20% methanol for 2h at 50V ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins of interest were revealed by Clarity Western Blot ECL substrate (Millipore).
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bioRxiv - Neuroscience 2021Quote: ... and protein was eluted with 100 μg/mL FLAG peptide (Sigma-Aldrich) in elution buffer for 1 hour at 4°C with rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... The chromatin solution was pre-cleared with protein A sepharose (Sigma-Aldrich), 10% retained (input ...
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bioRxiv - Immunology 2020Quote: ... Separated proteins were transferred to PVDF Immobilon-FL membranes (Millipore, Billerica, MA) using the BioRad Trans-Blot Turbo Transfer System (Hercules ...
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bioRxiv - Neuroscience 2021Quote: ... immunoreactive proteins were visualized using Immobilon® Classico Western HRP Substrate (Millipore) or Immobilon® Forte Western HRP Substrate (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Protein was reduced with tris (2-carboxyethyl) phosphine (Sigma-Aldrich, MO, USA) and alkylated with methylmethanethiosulphonate (MMTS ...
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bioRxiv - Physiology 2021Quote: ... All images were normalized to Amido Black total protein stain (A8181, Sigma) using the entire lane corresponding to each sample.
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bioRxiv - Biochemistry 2021Quote: ... Protein solution (4 mg/mL in 50 mM Tris-HCl (Sigma-Aldrich), pH 8.5 ...