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Citations for Millipore Sigma :
4751 - 4800 of 10000+ citations for Mouse AWAT2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... Mouse anti-actin monoclonal antibody (MAB1501, Millipore, RRID: AB_2223041) was used as an internal control ...
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bioRxiv - Immunology 2024Quote: ... mouse samples were stained with Masson Trichrome staining (Sigma), Congo red staining (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-γH2AX (Millipore, UpState, 05-636; 1:2,000); rabbit anti-RAD51 (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-Flag tag (Millipore Sigma, F3165, 1:5000). Membranes were scanned on a LI-COR Odyssey Imager ...
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bioRxiv - Cell Biology 2024Quote: ... anti-Flag (mouse, M2, F3165, 1:1000, Sigma-Aldrich), Cycloheximide (C7698 ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-mouse (50185, Sigma-Aldrich; A21426, Thermo Fisher scientific) and anti-goat (A11055 ...
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bioRxiv - Plant Biology 2024Quote: ... and goat anti-mouse-HRP (1:10,000, Sigma A0168). MAPK activation blots were probed with rabbit anti-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse anti-rabbit alkaline phosphatase (Sigma, catalog number A2556) diluted at 1:1000 ...
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bioRxiv - Systems Biology 2024Quote: ... and Ki67 (1:200 Millipore mouse-anti Ki67 # mab4190) antibodies were diluted in blocking solution and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and mouse tubulin (05-829, clone DM1a; Sigma-Aldrich). Membranes were washed with TBST for 5 min 3× at RT on a shaker ...
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bioRxiv - Microbiology 2024Quote: ... with HRP-conjugated anti-mouse antibodies (A6782; Sigma-Aldrich) at 1:20,000 as secondary antibodies for 1 h.
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bioRxiv - Neuroscience 2024Quote: ... Mouse anti ACTA2-Cy3 (1/400, Sigma Aldrich, C6198); Mouse anti CDH1 (1/200 ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-beta-Actin 1:5000 (Millipore Sigma A1978), mouse anti-TUBB3 1:5000 (BioLegend 801201) ...
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bioRxiv - Neuroscience 2024Quote: ... neuronal marker NEUN (1:200, mouse, EMD Millipore MAB377), SMAD4 (1:250 ...
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bioRxiv - Cell Biology 2020Quote: ... washed and incubated with mouse anti-HA primary antibody (1:2000; Covance, USA) and goat anti-mouse-HRP (1:5000; Sigma, Saint Louis, USA).
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bioRxiv - Cell Biology 2019Quote: ... anti PMF1/NNF1 (in house made mouse affinity purified monoclonal; clone RH25-1-54, 1:1000) and anti-Tubulin (mouse monoclonal, Sigma-Aldrich; 1:10000). Quantification of protein levels from western blots was performed with the following formula ...
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bioRxiv - Cell Biology 2019Quote: ... 1:5000), mouse α-tubulin (B-5-1-2, 1:10000) and mouse α-Vinculin (hVIN-1, 1:5000) were obtained from Sigma-Aldrich. Rabbit α-Cyclin B1 has been described before [46] ...
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bioRxiv - Genetics 2021Quote: ... Circulating thyroid-stimulating hormone (TSH) was measured in 10 μL serum via Milliplex MAP Mouse Pituitary Magnetic Bead Panel Mouse (EMD Millipore, Billerica, MA) on a Luminex 200 (Luminex Corporation ...
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bioRxiv - Biophysics 2020Quote: ... followed by 3× washes with PBS (mouse monoclonal anti-α-Tubulin, clone TUB-A4A Sigma Aldrich; mouse monoclonal tyrosine anti--Tubulin, clone TUB-1A2 Sigma Aldrich; rabbit polyclonal anti-β-III-Tubulin ...
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bioRxiv - Genetics 2020Quote: ... Protein– antibody interaction was detected with either anti-mouse or anti-rabbit peroxidase-conjugated mouse IgG antibody (Sigma-Aldrich, St Louis, MO, USA), using Amersham™ ECL Plus western blotting detection system (GE Healthcare Life Sciences ...
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bioRxiv - Genetics 2023Quote: ... Slides were incubated with goat anti-mouse collagen α1(XI) antibody at 1:500 dilution and mouse anti-rat PAX1(Cat #MABE1115M, Sigma-Aldrich, MO, USA), in PBS + 0.1% Triton + 1% normal goat serum at 4L°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% NaN3) and incubated with primary antibody (monoclonal α-V5-Tag mouse antibody /Elabscience/ and monoclonal α-Flag M2 mouse antibody /Sigma Aldrich/) and secondary antibody (AF488-conjugated goat anti-mouse antibody /Elabscience/) ...
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bioRxiv - Neuroscience 2024Quote: ... Both mouse and human brain sections were labeled for TH (mouse monoclonal, clone LNC1, 1:2000, Catalog No. MAB318, EMD Millipore, Burlington, MA) and VGLUT2 (rabbit polyclonal ...
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bioRxiv - Biophysics 2020Quote: ... The plasmids for p53-R248Q were transformed into Rosetta™ 2 (DE3) (Sigma Aldrich, 71397) by electroporation ...
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bioRxiv - Genetics 2021Quote: ... 100 ug/mL blasticidin S (to kill cells not expressing the blasticidin-resistant plasmid; Sigma), and 10 nM AP1903 (to kill un-integrated cells expressing the AP1903-sensitive caspase gene ...
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bioRxiv - Biophysics 2022Quote: The pET-19b plasmids encoding the CPB constructs were transformed into BL21 (DE3) strain (Sigma) for protein over-expression ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg of each minigene plasmid was delivered to cells using branched polyethylenimine reagent (Sigma) in FBS-free DMEM ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR product were assembled into KpnI and EcoRI difgested pZDonor-AAVS1 Puromycin plasmid (Sigma-Aldrich) and ligated using the Quick Ligase Kit (New England Biolab) ...
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bioRxiv - Immunology 2019Quote: ... Component DNA plasmids were transfected into Lenti-X-HEK-293T cells via GeneJuice® (Novagen) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Each plate was transfected with 20 μg of overexpression plasmid with 100 μg PEI (Sigma). After 48 h cells were collected in TBS ...
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bioRxiv - Microbiology 2021Quote: ... Expression was induced from the plasmids in BL21 cell induced with 0.2 mM IPTG (Sigma) overnight at 18°C ...
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bioRxiv - Biochemistry 2020Quote: ... the GAF domain (residues 164–313) of RcaE was cloned into plasmid pET-28 (Novagen). The construct was expressed in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Neuroscience 2020Quote: ... A plasmid solution (DNA concentration of 1 μg/μl + Fast-Green 1% (F-7258, Sigma)) was then injected in the lateral ventricles of the embryos with glass micropipettes ...
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bioRxiv - Cell Biology 2021Quote: ... coli DH10B cells and cells harboring recombinant plasmids selected on Luria Bertani (LB; Sigma-Aldrich) agar plates with 50 μg/ml kanamycin ...
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bioRxiv - Immunology 2022Quote: ... The sequence was cloned into the pET42a plasmid (Cat No 70561, Novagen, Merck, Overijse, Belgium) in frame with an N-terminal caspase3-cleavable glutathione S-transferase (GST)-tag (26) ...
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bioRxiv - Biophysics 2022Quote: ... A mixture of 37.5 µg of ABCA7EQ plasmid and 75 µg of polyethyleneimine (PEI, Sigma) was incubated for 15 min at room temperature before being applied to 15 ml plates of HEK293T cells at a confluency of 60 - 80% to initiate transfection and expression ...
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bioRxiv - Microbiology 2022Quote: ... All plasmids were maintained as strains and maintained at -80°C in 25% glycerol (Sigma).
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bioRxiv - Cancer Biology 2022Quote: ... Myc-CaP cells were transfected with pCas9-Rb1-CRISPR plasmid (Sigma-Aldrich, St. Louis, MO) using Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The pCMV-FLAG-His6-NPC1L1-MLD was cloned in the pFLAG-CMV-3 plasmid (Sigma). This construct contains a pre-protrypsin signal sequence ...
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bioRxiv - Neuroscience 2022Quote: ... A mixture of plasmid DNA (∼2 µg/µl) and 0.03 % fast green dye (Sigma Aldrich) was injected intraventricularly using micropipettes pulled from borosilicate glass capillaries (1.5 mm outer diameter ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was cloned into the NdeI and XhoI sites of plasmid pET21b (Novagen). To generate a plasmid for the expression of a cleavable GST tagged GbpA ...
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bioRxiv - Molecular Biology 2020Quote: A synthetic gene (Integrated DNA Technologies) for Nb806 was cloned into plasmid pET-26b(+) (Novagen) with a N-terminal His6 tag followed by a thrombin protease cleavage site ...
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bioRxiv - Neuroscience 2020Quote: ... and then 8-12 ug of plasmid mixed with Fast Green (2 mg/ml; Sigma) was injected into the lateral ventricle of embryos ...
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bioRxiv - Genetics 2021Quote: ... Lentiviral expression vectors were purified with GenElute HP Plasmid Maxiprep Kit (Sigma, St-Louis, MO) and only plasmid preparations with absorbance (260/280 ...
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bioRxiv - Cell Biology 2021Quote: ... 7.5μg of pSPAX2 and 4μg of pVSVG packaging plasmid using standard calcium precipitation protocol (Sigma) and grown in 20% serumcontaining medium for 24h ...
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bioRxiv - Biochemistry 2021Quote: ... and 100 ng of pcDNA A3C-3xHA expression plasmid using GeneJuice transfection reagent (EMD Millipore). The SIVsmm lineage 5 (L5 ...
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bioRxiv - Neuroscience 2022Quote: A mix of endotoxin-free plasmid preparation (0.5 mg/mL) and 0.5% Fast Green (Sigma) was injected into one lateral hemisphere of E15.5 embryos using FemtoJet 4i (Eppendorf) ...
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bioRxiv - Neuroscience 2022Quote: A mix of endotoxin-free plasmid preparation (2 mg/mL) and 0.5% Fast Green (Sigma) mixture was injected using FemtoJet 4i (Eppendorf ...
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bioRxiv - Microbiology 2024Quote: ... 293T cells were transfected with gene drive plasmids precomplexed with PEI (408727-100ML; Sigma[Aldrich), cultured for 12 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... and the RDF plasmid encoding the RD114 envelope) using the GeneJuice transfection reagent (Merck Millipore). Viral supernatant was collected 48 and 72 hours after transfection ...