Labshake search
Citations for Millipore Sigma :
4701 - 4750 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... Membranes were stained with Fast Green FCF (Sigma-Aldrich) and imaged for total protein on a ChemiDoc MP Imaging System (Bio-Rad) ...
-
bioRxiv - Physiology 2024Quote: ... membranes were blocked with 5% bovine serum albumin (Sigma) and incubated overnight at 4°C with primary antibodies directed against Snai1 (CST#3879 ...
-
bioRxiv - Physiology 2024Quote: ... The membranes were stained with Ponceau S (Sigma-Aldrich) to verify equal protein loading across lanes.
-
bioRxiv - Microbiology 2024Quote: ... sealed with Breathe-Easy® sealing membrane (Sigma Aldrich), and grown at 37°C for 24 hours with shaking every 15 minutes ...
-
bioRxiv - Immunology 2024Quote: ... which was followed by transfer onto PVDF membranes (Millipore) and blocking for 1 h at room temperature (RT) ...
-
bioRxiv - Genetics 2024Quote: ... membranes were incubated Luminata Forte Western HRP substrate (Millipore) before visualization using a BioRad ChemicDoc XRS+ or X-OMAT-Film processor using Hyperfilm™ ECL (GE Healthcare).
-
bioRxiv - Cell Biology 2024Quote: ... The resolved samples were transferred to PVDF membranes (Millipore). The membranes were incubated with the primary antibodies listed in Key Resources Table ...
-
bioRxiv - Neuroscience 2024Quote: ... the membranes were washed in 0.05% Tween (P1379, Sigma) in PBS (PBST ...
-
bioRxiv - Plant Biology 2024Quote: ... filtered with 0.45 µm PVDF membrane filter (Millipore, SLHV033RS) and loaded onto Ni-NTA columns (QIAGEN ...
-
bioRxiv - Neuroscience 2020Quote: ... Tau protein ladder (Sigma) was used in some experiments as a control ...
-
bioRxiv - Biophysics 2022Quote: ... BLUeye protein ladder (Sigma) was used as a standard ...
-
bioRxiv - Biophysics 2022Quote: ... standard proteins BSA (Sigma), Apoferritin (Sigma ...
-
bioRxiv - Plant Biology 2022Quote: Protein A-sepharose (Sigma) was washed twice with 5 column volumes of buffer HNTG [20 mM HEPES-NaOH pH 7.5 ...
-
bioRxiv - Microbiology 2020Quote: ... Protein disulfide isomerase (Sigma) was used in positive control reactions and albumin (Research Organics ...
-
bioRxiv - Immunology 2021Quote: ... plus protein A (Sigma).
-
bioRxiv - Biochemistry 2024Quote: ... S protein agarose (Millipore) was added to each sample ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Protein standard (Sigma: P5369) was used as standard (ranging from 0-0.5mg/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... ECL protein detection (Millipore) was performed with a Fusion Fx7 chemiluminescent imager ...
-
bioRxiv - Biochemistry 2024Quote: ... Protein-A agarose (Sigma), Streptavidin agarose (ThermoFisher) ...
-
bioRxiv - Immunology 2024Quote: ... protein G beads (Millipore) were used (2 hours ...
-
bioRxiv - Microbiology 2019Quote: ... ELISAs of cells harvested from tryptone plates were treated with 100 Kunitz units/mL DNaseI (D5025, Sigma Aldrich) for 1 hr statically at 37 °C ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... IL-1β and TNF-α using indirect sandwich enzyme-linked immunosorbent assay (ELISA) (Sigma-Aldrich; #RAB0308 and #RAB0477) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2024Quote: One rabbit was immunized with 500 μg of recombinant arpin by subcutaneous injection of an emulsified 1:1 mixture of protein and TiterMax Gold (Sigma Aldrich; St. Louis, MO). Three more immunizations were performed 14 ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were normalized to either actin or GAPDH housekeeping proteins with monoclonal antibodies (anti-actin; 1∶1000, Sigma or anti-GAPDH; 1:10,000 Abcam).
-
bioRxiv - Bioengineering 2024Quote: ... and incubated for 1 h in a 1:1 mixture of 100 % Ethanol and 100 % Epon 812 substitute (Epoxy embedding kit 45359, Sigma-Aldrich). After an overnight incubation with 100 % Epon in 1.5 mL Eppendorf tubes with open lids ...
-
bioRxiv - Biochemistry 2021Quote: ... 30 KDa for T-protein and L-protein and 100 KDa for P-protein) were purchased from Millipore (Billerica, MA, USA). Mut Express II Fast Mutagenesis kit V2 was purchased from Vazyme (Jiangsu ...
-
bioRxiv - Biochemistry 2020Quote: ... The membranes were blocked for 1 h in Tris-buffered saline (TBS) with 5% milk and 0.05% Tween 20 (Sigma-Aldrich, St. Louis, MO) and washed 3 times (5 min each ...
-
bioRxiv - Cancer Biology 2020Quote: ... 2uL of lysate (approximately 2ug) was spotted directly onto 0.2 um nitrocellulose membranes and blotted with primary antibodies against puromycin (Sigma MABE343 1:25,000 dilution) and vinculin (Abcam ab18058 ...
-
bioRxiv - Biochemistry 2022Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich, Cat No A2179) in PBST ...
-
bioRxiv - Immunology 2021Quote: ... Each membrane was re-used to for the loading control vinculin (mouse-a-hVCL, clone hVIN-1, 116 kDa, Sigma-Aldrich, cat#V9131). As secondary antibodies ...
-
bioRxiv - Neuroscience 2023Quote: To label AMPA receptors in the plasma membrane the N-terminal extracellular domain of GluA2 was stained with monoclonal mouse anti-GluA2 (Millipore, # MAB397, 1:500) for 2 hours without prior permeabilization ...
-
bioRxiv - Pathology 2024Quote: ... The membranes were incubated after washing with an anti-rabbit IgG alkaline phosphatase secondary antibody (1:5000, Sigma– Aldrich, St Louis, MO, USA) for 1 h ...
-
bioRxiv - Physiology 2024Quote: ... and the membrane was incubated for 2h at RT with rabbit anti-GAPDH antibody (Sigma Aldrich, G9545, diluted 1:2000 in TBS-T) at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... The plates were centrifuged for 7 minutes at 2000rpm for higher transfection efficiency and were incubated for 1-3 days with coving by Breathe-Easy sealing membrane only (Sigma-Aldrich, # Z380059-1PAK). All assays were performed on Day 1-3 following transfection ...
-
bioRxiv - Biophysics 2023Quote: ... Stretching is performed using 2x2 cm flexible polydimethylsiloxane (PDMS) membranes (Sylgard184; crosslinker-to-base ratios of 1:14; cured for 24 h at 65°C, Sigma-Aldrich, St. Louis) as described in (45) ...
-
bioRxiv - Neuroscience 2023Quote: ... The same procedure was then applied to the same membranes with anti-tubulin antibodies (mouse anti-tubulin, 1:40000, Sigma Aldrich cat. # T16199). The molecular weight ladder corresponds to Invitrogen SeeBlue™ Plus2 (reference LC5925).
-
bioRxiv - Cell Biology 2022Quote: ... Membranes were then washed with PBST for 5 min and incubated with the following primary antibodies: GAPDH (EMD Millipore, #MAB 374, 1:2000), β-catenin (EMD Millipore ...
-
bioRxiv - Cell Biology 2023Quote: ... was electroblotted (25 V and 1 mA for 20 min) under semi-dry conditions onto a positively charged Hybond®-N+ hybridization membrane (Millipore Sigma, GERPN203B) using a Trans-Blot® TurboTM Transfer System (Biorad ...
-
bioRxiv - Neuroscience 2024Quote: ... the membrane was washed with TBST buffer and the membrane was set for 2 hours at room temperature with the secondary goat anti-rabbit IgG HRP antibodies (Sigma Aldrich; 1:10000) diluted in a TBST buffer with 3% PVP ...
-
bioRxiv - Molecular Biology 2020Quote: ... protein was isolated from cells using 300μl CytoBuster™ Protein Extraction Reagent (Novagen) per well according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... Lysates were sonicated and protein concentration was determined using BCA protein assay (Sigma). 30 μg of total proteins were loaded on 12% SDS–PAGE gel and separated by electrophoresis ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... a marker of protein oxidation (protein carbonyls; OxyBlot; s7150; EMD Millipore, Billerica, MA), and a marker of mitochondrial biogenesis (PGC-1α ...
-
bioRxiv - Immunology 2020Quote: Total proteins isolated from BMDCs were lysed in CytoBuster protein extraction reagent (Novagen). Protein quantification was performed using NanoDrop One Spectrophotometer ...
-
bioRxiv - Cell Biology 2022Quote: ... the other stained for proteins with SYPRO Red Protein Gel Stain (Sigma Aldrich). Both dyes were used according to the manufacturers’ protocols.
-
bioRxiv - Molecular Biology 2024Quote: The protein mixtures were filtered using a low protein binding filter (Millipore Sigma) and purified on a SourceQ15 anion exchange column (Cytiva ...
-
bioRxiv - Genetics 2024Quote: ... protein concentration was measured by use of a BCA Protein Assay (SIGMA Aldrich) and samples stored at -80°C.
-
bioRxiv - Genomics 2024Quote: ... Protein transfer and total protein content was assessed by Ponceau Staining (Sigma Aldrich). Ponceau Stain was washed out with 1x TBST ...
-
bioRxiv - Microbiology 2022Quote: ... total cell protein was measured using the bicinchoninic acid protein assay (Sigma-Aldrich) against bovine serum albumin standards ...
-
bioRxiv - Microbiology 2023Quote: ... or with slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose). For GFP-p65 and GFP-COMMD2 the samples were immunoprecipitated as per manufacturer’s instructions with the elution was recovered in either 5x with Laemmli buffer 74 and boiled at 98°C (dry bath ...
-
bioRxiv - Microbiology 2023Quote: ... 1000 µg of protein extracts were cleared with agarose protein A beads (Millipore) at 4 ℃ for 1.5 h on a rocking platform ...