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Citations for Millipore Sigma :
4701 - 4750 of 10000+ citations for Catenin alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was then washed three times in PBS-T buffer and incubated with HRP-conjugated anti-rabbit antibody (1:10,000, Sigma) for 4 h ...
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bioRxiv - Microbiology 2023Quote: ... The primary antibodies were followed by respective species-specific secondary antibodies conjugated to HRP (Anti rabbit, 1/20000, Sigma) or (Anti mouse ...
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bioRxiv - Molecular Biology 2023Quote: ... A goat anti rabbit secondary antibody (1:30000) conjugated to alkaline phosphatase (Cat#A3687, Millipore Sigma, Burlington, MA, USA) and western blue stabilized substrate for alkaline phosphatase (Promega ...
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bioRxiv - Microbiology 2023Quote: ... The secondary horseradish peroxidase (HRP)-conjugated antibodies directed against rabbit or mouse were used at a 1/20,000 dilution (Sigma). Detection of luminescent signal was performed with a Luminata Western HRP substrate kit (Millipore).
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bioRxiv - Microbiology 2023Quote: ... This was followed by probing with secondary HRP conjugated anti-mouse or anti-rabbit antibody (1:2000 dilution, SIGMA). Proteins were visualized using the Clarity Western ECL substrate (Bio-Rad 1705060EM ...
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bioRxiv - Plant Biology 2023Quote: ... for detection of FLS2-BAK1 co-immunoprecipitation or anti-rabbit IgG (whole molecule)– HRP (A0545, Sigma, dilution 1:10000) for all other western blots.
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then incubated with Alexa Fluor 555 donkey anti-rabbit antibody (1:500 dilution; Abcam in 10% horse serum in PBS with 0.1% Triton X-100, (Sigma)) ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were incubated at 4°C for 48h with rabbit anti-cFos primary antibody (1:3000, Millipore Sigma ABE457) and mouse anti-RFP primary antibody (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... and probed for the mitochondrial marker TOM20 with an anti-TOM20 rabbit polyclonal antibody (1:1000 dilution; Sigma-Aldrich) and anti-cytochrome c mouse monoclonal antibody (1:500 dilution ...
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bioRxiv - Plant Biology 2023Quote: ... Secondary antibody incubation was performed for 30min with 1:10000 (v/v) anti-rabbit IgG-peroxidase antibody (Sigma-Aldrich). Again ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit polyclonal antibody against non-muscle myosin heavy chain IIA (1:200 for immunofluorescence; Sigma-Aldrich; catalogue number M8064)
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bioRxiv - Neuroscience 2023Quote: ... we used an anti-cholecystokinin (26-33) antibody raised in rabbit (C2581, Sigma-Aldrich, St. Louis, MO; 1:5,000). After antigen retrieval with HistoVT (Nacalai Tesque ...
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bioRxiv - Neuroscience 2023Quote: ... The following secondary antibodies were used for staining: Goat anti-rabbit 647 (1:1000, Sigma-Aldrich, 40839-1ML-F), Goat anti-mouse 488 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Then neurons were fixed and labelled with anti-Synapsin I antibody (Rabbit, dil. 1:1000, Millipore cat. no. AB1243), and revealed by anti-rabbit secondaru antibodies (Alexa 488 made in goat ...
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bioRxiv - Cancer Biology 2024Quote: ... Detection of specific protein bands was accomplished using a rabbit monoclonal anti-GLRX3 antibody (1:1,000, HPA028941, Sigma-Aldrich), ferritin (1:1,000 ...
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bioRxiv - Neuroscience 2024Quote: ... before being incubated in primary antibody (rabbit anti-γH2A.X, Cat#9718, Cell Signalling, 1:1000) containing 5% bovine serum albumin (BSA, Sigma) TBST ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were incubated with the following primary/secondary antibody pairing: PCP4 (1:300, rabbit anti-PCP4 #HPA005792, Sigma-Aldrich)/goat anti-rabbit IgG (Life Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... mitotically active cells were identified using a rabbit anti-phosphohistone-3 antibody (Ser10, Millipore 06–570, 1:500 dilution), with a Cy3-donkey anti-rabbit immunoglobulin G secondary antibody (the Jackson laboratories ...
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bioRxiv - Physiology 2024Quote: ... Sections were incubated o/n (4°C) with primary antibody against Laminin (Rabbit polyclonal, Sigma, L9393, diluted 1:20). Alexa Fluor Donkey anti-Rabbit 488 (A11034 ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples were separated on 12% non-reducing SDS-PAGE gel and cysteine-dimedone protein adducts visualised by western blot analysis using 1:10,000 diluted rabbit anti-cysteine sulfenic acid primary antibody (Merck Millipore) and 1:10,000 diluted horseradish peroxidase conjugated goat anti-rabbit IgG secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... Approximately 5 mg of extract containing the corresponding recombinant protein was incubated with 25 µl of glutathione sepharose (SIGMA) and subsequently mixed with yeast extracts expressing Sen1 TAP-Nter (typically 0.5 mg of extract per binding reaction) ...
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bioRxiv - Neuroscience 2021Quote: ... parental SK-N-SH cells were trypsinized and resuspended in grafting media in presence of 10μg/ml recombinant NXPH1 (rNXPH1) or 100μg/ml BSA (Ctrl; SIGMA #A7906). To test the growth of NB cells depleted from their α-NRXN1+ subpopulation ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Pathology 2020Quote: ... each recombinant tau isoform was thawed at room temperature and filtered with a 100 kDa spin column filter (Millipore) to remove unwanted tau aggregates ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were loaded to have roughly similar band intensity for the band of interest to enable better estimation of relative protein size (recombinant pro-conA: 0.75 μg, conA (Sigma): 1.5 μg ...
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bioRxiv - Plant Biology 2019Quote: ... The recombinant proteins with either FLAG-tag or His-tag were purified by corresponding immune affinity agarose beads (Sigma).
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bioRxiv - Cell Biology 2021Quote: BMDC and BMLC maturation was induced by overnight treatment with 100 ng/ml recombinant murine TNFα (Sigma-Aldrich, USA). In some experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Rose-John), 20 U/ml recombinant murine GM-CSF (PharmedArtis, Germany) and 10−6 M dexamethasone (Sigma-Aldrich, USA). Progenitor cells were amplified 5 to 11 days before differentiation into DC was induced by supplementing 200 U/ml recombinant murine GM-CSF ...
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bioRxiv - Biophysics 2022Quote: ... a heparosan trisaccharide amine derivative was extended with recombinant MBP-PmHS1 synthase using UDP-GlcNAc and UDP-GlcA (Sigma) [48] ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich). After 24 hours of CHIR99021 stimulation ...
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bioRxiv - Microbiology 2022Quote: ... coli BL21 (DE3) was used as the host for expressing recombinant proteins encoded in the pET30a vector (Novagen, USA). Appropriate antibiotics were added when needed at the following concentrations ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged recombinant proteins were detected using an HRP-conjugated anti-His monoclonal antibody (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... beads were removed and recombinant dynein was concentrated in a 100 KDa cut-off filter (Amicon Ultracel, Merck-Millipore) to a final volume of 500 μL ...
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bioRxiv - Cell Biology 2021Quote: ... We dissected ovaries in live imaging media (Schneider’s Drosophila medium containing 15% fetal bovine serum (FBS) and 200 mg/mL recombinant human insulin (Sigma)) ...
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bioRxiv - Immunology 2020Quote: DHFR activity of the recombinant β-lactamase-DHFR fusion protein was evaluated using the DHFR Assay Kit (Sigma-Aldrich), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... centrifuged 20 min at 20000 g and recombinant GST-tagged proteins were purified using the GST-BindTM Kit (Novagen). To synthetize the NOVA2 RNA target ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3) cells (Novagen). Protein expression was induced during logarithmic growth through the addition of 0.5mM isopropyl-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2022Quote: Expression of recombinant extracellular proteins was performed by transient transfection in HEK−293F cells using polyethylenimine (PEI, Sigma Aldrich). 300 mL of culture at a density of 1.0 × 106cells/mL was transfected with 300 μg DNA using 450 μg PEI ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: Recombinant human MMP-2 (R&D) and MMP-9 (R&D) were activated using p-aminophenylmercuric acetate (APMA, Sigma) in MMP assay buffer (50 mM Tris (Fisher) ...
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bioRxiv - Developmental Biology 2024Quote: ... as controls and analyzed for promoter activation in the presence of 3mg/l recombinant human SPARC (SRP3159, Sigma-Aldrich) using the Dual-Luciferase® Reporter Assay System (E1910 ...
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bioRxiv - Cell Biology 2023Quote: ... imidazole 5 mM and recombinant RAD51-proteins were purified on a NiNTA resin (#P6611, Sigma, St Louis, MO, USA) using the washing buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Mouse primary cortical cultures were then treated with 0.5 μg/ml human recombinant tissue inhibitor of metalloproteinase-2 (TIMP2, Sigma) for one biological replicate or 10 μM BB94 for two biological replicates ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing recombinant PKAc verified via SDS-PAGE were pooled and concentrated to 250μL using an Amicon concentrator (10K MWCO; Millipore). The concentrated sample was then applied to a preparative-grade Superdex200 column (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... was added to wells containing coated coverslips and left 48 hours for cells to settle before fixation in 4% paraformaldehyde and immunostaining overnight with mouse anti-AGO2 mAb (Sigma Aldrich 04-642 clone 9E8.2) or rat anti-AGO2 mAb (Millipore MABE253 clone ...
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bioRxiv - Cancer Biology 2020Quote: ... rabbit anti-T7 (AB3790) and rabbit anti-H3K9/18Ac (07-593) (Millipore sigma, Burlington, MA).
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bioRxiv - Pathology 2022Quote: ... rabbit α-TRP120-I1 (59) polyclonal rabbit α-TRX (T0803; Sigma-Aldrich, Saint Louis, MO). Polyclonal rabbit anti-TRP120 antiserum was commercially generated against a TRP120 epitope inclusive of aa ...
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bioRxiv - Cell Biology 2020Quote: ... were subjected to immunoblot analysis with specific primary antibodies (Table 1) and secondary antibodies (anti-mouse/rabbit IgG coupled to horseradish peroxidase; Sigma Aldrich; 1:5000 dilution in blocking buffer). Detection of chemi-luminescent signal was performed with Clarity ECL Western Blotting substrate (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked and incubated with primary antibodies rabbit polyclonal anti-Rab35 (Antibody Facility MPI-CBG Dresden, F18256, 1:1000) and mouse monoclonal anti-γ-tubulin (Sigma-Aldrich, Cat. No. T6557, 1:2000) and secondary HPR-conjugated antibodies (1:10000 ...
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bioRxiv - Immunology 2022Quote: ... The pseudotyped virus-mAb mixture were then overlayed onto target cell lines in the presence of 5 μg/ml polybrene (Sigma Aldrich, catalog no. TR-1003-G) and analyzed 48 h post-infection ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used for immunoblot (IB) and immunofluorescence (IF) studies: rabbit anti-Actin (IB, 1:1000, Sigma A2066), mouse anti-CDC27 (IB ...