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Citations for Millipore Sigma :
4701 - 4750 of 10000+ citations for 6 METHOXY 1 2 3 4 TETRAHYDRO NAPHTHALENE 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... The powdered food mix was added in a 1:1 ratio (grams:mL) to a 2% agar (Sigma #A1296) solution in water before solidification to a semi-solid consistency that lessens the potential for hoarding of food in group housing of mice and increases accuracy of measuring food consumption as large chunks can’t be taken at one time and the food doesn’t crumble like with standard rodent chows ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... DMEM without serum contained 1 µg of L-1- tosylamide-2-phenylethyl chloromethyl ketone-treated trypsin (TPCK; Sigma) for activation of the virus infectivity ...
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bioRxiv - Cancer Biology 2023Quote: ... the viral medium was removed and the wells overlaid with a 1:1 solution of 2% agar (Sigma) and 2X DMEM containing 5% (v/v ...
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bioRxiv - Neuroscience 2024Quote: LL-341070 was provided by Autobahn Therapeutics as a solution in 1% 1-methyl-2-pyrrolidone (Sigma 270458) and 1% Kolliphor HS 15 (Sigma 42966 ...
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bioRxiv - Physiology 2023Quote: ... the gonads were incubated overnight in the dark at 4 °C with secondary goat anti-rabbit IRDye 680RD-conjugated antibody (LiCor) 1:1000 in PBST/0.1% BSA and 1 ng/µl 4,6-diamidine-2-phenylindole (DAPI, Sigma). The gonads were mounted on 2% agarose pads in Fluoromount-G (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation of secondary antibodies (1:500) for 2 h and DAPI (1:10,000, D9542, Sigma-Aldrich) in PBS for 5 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were carried out using the indicated antibodies: α-Tubulin (Sigma, B-5-1-2; T5168, 1:100,000), BLM (Bethyl ...
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bioRxiv - Bioengineering 2023Quote: ... b-mercaptoethanol and 1000 U ml-1 LIF) and 2 ug ml-1 doxycycline (cat. 33429 Sigma-Aldrich). MEFs were reprogrammed in the presence of doxycycline for 12 days and without doxycycline for the last 5 days ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells in Accutase were added 1:2 to mTeSR™1 with 5 µM Blebbistatin (Bleb, B0560, Sigma), and pelleted at 150 g for 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... 25 mg of minced tissue was digested on ice for 4 minutes in 1 ml of Collagenase/Elastase/Dispase enzyme mix (6 mg/ml Collagenase A (Sigma, 10103578001), 4.3 units/ml Elastase (Worthington ...
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bioRxiv - Immunology 2024Quote: ... The minced tumor tissue was transferred to a 6-well plate containing 3 mL/well of tumor digestion media (NK cell media + 1 mg/mL collagenase IV (Sigma, cat. C5138) + 0.2 mg/mL DNAse I (Roche ...
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bioRxiv - Genomics 2022Quote: The stable cell lines constantly expressing the minigenes were subjected to four time points (0, 3, 6, and 9 h) of post actinomycin D treatment (final concentration of 1 μg/mL; Sigma, no. A9415) treatment in three biological replicates ...
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bioRxiv - Neuroscience 2019Quote: ... were expressed in 3T3 cells and IPed using 1µg anti-Fyn (mAb, sc-434, SantaCruz) or 4 µg anti-MAP2 (HM-2, mAb, Sigma, Cat # M4403) and Protein G beads (CalBiochem) ...
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bioRxiv - Immunology 2021Quote: ... mice were anaesthetised using inhaled isoflurane (4% (v/v) isoflurane and 2 l O2/min) and 30 μl of LPS (250 μg/mL, Sigma-Aldrich) or vehicle PBS were administered intranasally ...
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bioRxiv - Biochemistry 2021Quote: ... at a flow rate of 10 µL/min for 4 min in an isocratic mode of Mobile Phase C (2% acetonitrile (Sigma-Aldrich), 0.1% formic acid) ...
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bioRxiv - Biophysics 2020Quote: ... Samco Trident Vial Tall with PP Screw Cap) were incubated for 4 hours with 2 % aqueous Tween solution (Tween20, Sigma-Aldrich), then rinsed with deion ...
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bioRxiv - Biochemistry 2021Quote: MDA-MB-231 cells were lysed with RIPA buffer for 2 h at 4°C and tissues were disrupted and lysed in RIPA buffer (Sigma-Aldrich) using a MagNA Lyser instrument (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... Supernatant protein concentrations were adjusted to 5 mg/mL and incubated for 2 h at 4 °C with 200 μL of anti-FLAG matrix (Sigma-Aldrich) for co-immunoprecipitation with FLAG-RBOHD and with 200 μL of anti-HA magnetic beads (Miltenyi Biotec ...
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bioRxiv - Biophysics 2020Quote: ... maintains a pluripotent state of the stem cells.2–4 Cells were cultured in Glasgow modified Eagle’s medium (GMEM, Sigma Aldrich, Germany) supplied with 5.5 ml non-essential amino acids (Gibco Science ...
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bioRxiv - Bioengineering 2022Quote: ... A 2 μL aliquot of 100 μM aptamer in PBS was thawed and mixed with 4 μL of 10 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich) for 8 hours to reduce the disulfide bond ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.2% Triton X-100 and then incubated overnight at 4°C with a mouse anti-tyrosine hydroxylase antibody (TH, Sigma, T1299) at 1:200 dilution in PBS containing 1.5% BSA and 0.2% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... Embryos at 2 dpf (days post-fertilization) were removed from their chorions and anesthetized with 4% tricaine (MS-222, Sigma Aldrich). The embryos were placed in a 1% agarose-coated dish with small holes ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were fixed with 4% formaldehyde for 20 min and stained with 1x crystal violet solution (2% crystal violet (Sigma-Aldrich), 20% ethanol ...
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bioRxiv - Cell Biology 2019Quote: ... The sonicated lysate was incubated for 2 hours at 4 ° C with beads (Glutathione High Capacity Magnetic Agarose Beads, Sigma-Aldrich) previously washed in Rac buffer (50 mM Tris HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... Afterwards the medium was switched and cells were cultivated for 4 days in DMEM containing 10% FCS and 2 μM insulin (Sigma Aldrich) to induce adipogenic differentiation ...
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bioRxiv - Cancer Biology 2020Quote: Non-synchronized and synchronized cells were fixed using 70% Ethanol for 48 hours at 4°C then washed with PBS+2% goat serum (Sigma, UK) and resuspended in 1mL of ice-cold PBS ...
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bioRxiv - Immunology 2020Quote: ... 150 mM NaCl and 0.1% Tween-20 or TBST buffer, 2 h) and incubated (4°C, overnight) with biotinylated BSI-B4 lectin from Bandeiraea (Griffonia) simplicifolia (Sigma-Aldrich, #L3759-1MG ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-cleared nuclear lysate was then added to 50 μL beads conjugated with 2-4 μg primary IgG antibody: rabbit anti-APOBEC2 (Sigma, HPA017957) or rabbit IgG isotype control ...
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bioRxiv - Plant Biology 2021Quote: ... rinsed with 3 mL of medium and washed off into new Eppendorf tubes with 600 μL of boiling buffer (100 mM Tris-HCl, 4 mM EDTA, pH 7.5, autoclaved and supplemented with phosphatase inhibitor cocktail 2 (Sigma-Aldrich, P5726)) ...
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bioRxiv - Microbiology 2019Quote: NA activities of recombinant AIV strains were measured using the fluorogenic substrate MUNANA (2’-[4-Methylumbelliferyl]-alpha-D-N-acetylneuraminic acid; Biosynth and Sigma-Aldrich), as described previously [70] ...
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bioRxiv - Developmental Biology 2020Quote: ... After 4 days, cells were switched to DMEM/F-12, HEPES (31330038, Thermofischer Scientific) with 2% Horse serum (H1270, Sigma-Aldrich). Before staining ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% formaldehyde for 20 min and stained with 1x crystal violet solution (2% crystal violet (Sigma-Aldrich), 20% ethanol ...
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bioRxiv - Bioengineering 2021Quote: BMOs were collected from the array as described above and fixed with 2 % or 4 % PFA or 10 % Formalin (Sigma, HT501128) overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... central back skin of K14ΔNβ-cat x PDGFRαH2BEGFP transgenics was clipped and treated topically with 100 µl 4-Hydroxytamoxifen (4OHT) (2 mg/ml dissolved in acetone; Sigma-Aldrich) every second or third day for a total of 6 applications before and after sham or acUVB exposure (see experimental design in Figure 5C) ...
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bioRxiv - Genomics 2022Quote: ... bead/chromatin complexes were washed twice with Low Salt Wash Buffer at 4°C (Final 20 mM Tris-HCl, pH 8,0; 2 mM EDTA; 150 mM NaCl (Sigma-Aldrich, S6546); 1% Triton-X ...
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bioRxiv - Molecular Biology 2022Quote: ... 34 mg/mL chloramphenicol) and 2 μL DMSO containing 100x desired final concentration of 5-aminoimidazole-4-carboxyamide-ribonucleoside (Z) (Millipore Sigma) in 2 mL 96-well culture blocks ...
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bioRxiv - Neuroscience 2024Quote: ... The coverslips were then washed again for 2 min with the same 4% poly-acrylamide (PA) gel solution supplemented with the polymerizing agents ammonium persulfate (Sigma, A3678) and TEMED (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... they were cryopreserved for 48h at 4°C in a 30% sucrose solution and subsequently frozen with 2-methylbutane solution (Sigma-Aldrich). Parallel free-floating coronal sections (30-µm-thick ...
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bioRxiv - Cell Biology 2024Quote: ... diluted in DMEM without FBS and red phenol supplemented with 2 mM L-glutamine and 25 µM verapamil (V4629, CAS 152-11-4, Sigma Aldrich) for 1 h at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride) and lysed by the addition of 10 mg hen egg white lysozyme (Sigma, L6876) and sodium deoxycholate (Acros Organics ...
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bioRxiv - Neuroscience 2023Quote: ... 10 μm tissue sections (2-4 sections per donor) were fixed with 10% neutral buffered formalin solution (catalog no. HT501128, Sigma-Aldrich) for 30 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... the cell lysate was incubated (4 °C for 2 h) with anti-V5 agarose affinity gel antibody produced in mouse (Cat# A7345 from Millipore Sigma). After three repetitive washes (10 min each time ...
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bioRxiv - Cell Biology 2023Quote: ... Minced samples were transferred into a digestion buffer (4 mL digestion buffer per 100 mg of muscle) containing 2 mg/ml Dispase II (D4693 Sigma-Aldrich) and 2 mg/ml Collagenase IV (17104019 Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... or MC38 murine colon adenocarcinoma cells (2 × 105) into their shaved flanks (Day 0) and intraperitoneally injected with tamoxifen (4 mg/mouse in corn oil) (Sigma-Aldrich) on day 0 ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed with 4% formaldehyde for 20 min and stained with 1x crystal violet solution (2% crystal violet (Sigma-Aldrich), 20% ethanol ...
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bioRxiv - Cancer Biology 2022Quote: ... 12-well non-tissue culture treated plates were coated with 32 μg/mL retronectin (WEHI) in PBS solution overnight at 4°C then blocked with 2% bovine serum albumin solution (#A1595 Sigma-Aldrich) in PBS at 37°C for 30 min were prepared ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 mg of the whole cell extract was incubated for 2 h at 4°C with 15 μl of M2-anti Flag agarose (Sigma- Aldrich), GFP-trap agarose (Chromo Tek ...
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bioRxiv - Neuroscience 2023Quote: ... was changed daily and animals over 4 days post fertilisation were euthanized by overdose of MS-222 (300 mg/l) or 15% 2-Phenoyethanol (77699 SIGMA-ALDRICH) at the end of experiments.
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bioRxiv - Neuroscience 2023Quote: ... buspirone hydrochloride (1078-80-2) and all-trans retinal (116-31-4) were purchased from Sigma Aldrich (St Louis, MO, USA).(Dunham & Venton ...
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bioRxiv - Bioengineering 2023Quote: ... Each EC-patch was transferred by a wide bore pipette tip into 50 μL EGM-2 medium containing 4 U/mL thrombin (Sigma, T9549). Then the medium with a patch was mixed with 50 μL fibrinogen stock solution ...