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Citations for Millipore Sigma :
4651 - 4700 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the protein was expressed using a pETBlue-1 (Novagen) vector in E ...
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bioRxiv - Biophysics 2024Quote: Horse Myoglobin protein was purchased from Sigma Aldrich (M1882) and 22.6 mg was resuspended with 700 µL of NMR buffer (20 mM MES pH 6.2 buffer ...
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bioRxiv - Cancer Biology 2024Quote: Proteins were first reduced with 10 mM dithiothreitol (Sigma) for 1 hour at 56°C and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Sigma) and non-specific binding was blocked with 5% milk powder or 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was measured with Bradford reagent (Sigma-Aldrich), and 5 mg of the worm lysate was added to anti-Rabbit IgG magnetic Dynabeads (Fisher ...
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bioRxiv - Biophysics 2024Quote: ... Purified proteins were concentrated using Amicon Ultra filters (Millipore) to a concentration of 60 mg/ml.
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred on polyvinylidene (PVDF) (Sigma #P2938) or nitrocellulose (Amersham ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to Immobilon FL PVDF membranes (Millipore), and membranes were blocked using Intercept Blocking buffer (Li-Cor) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were competitively eluted using 3X FLAG peptide (Sigma; in 10 mM Tris HCl ...
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bioRxiv - Neuroscience 2024Quote: ... rat anti-myelin basic protein (MBP, Millipore, MAB386, RRID:AB_94975), rabbit anti-laminin (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... Protein G Sepharose 4B fast flow (P3296, Sigma Aldrich) was used for the Co-immunoprecipitation and ChIP assay ...
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bioRxiv - Cell Biology 2024Quote: ... Protein signals were developed and detected by chemiluminescence (Millipore) on a ChemiDoc MP Imaging System (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... with 800 ng of yeast enolase protein (Sigma-Aldrich) added as an internal protein control ...
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bioRxiv - Cell Biology 2024Quote: Total cell protein was extracted using RIPA buffer (Sigma) supplemented with protease inhibitors (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... and one Roche Complete protein inhibitor tablet (Millipore, 11873580001). Cells were then incubated with activated concanavalin-A beads (Bang Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were transferred to PVDF membranes (Merck Millipore), and Ponceau S (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: N-terminally His6-Smt3-tagged proteins (pRSFDuet-1, Novagen) were expressed in Rosetta (DE3 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined using the Bradford assay (Sigma), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... and protein G-sepharose CL-4B beads (Sigma-Aldrich). After SDS-PAGE and immunoblotting ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were blotted onto Immobilon-FL PVDF membranes (Millipore), which was subsequently blocked for 1 hour at room temperature using a blocking buffer consisting of 1 part Intercept Blocking Buffer (LI-COR Biosciences ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated with Amicon Ultra-15 (Millipore, USA) to a final concentration 9 mg/ml.
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bioRxiv - Plant Biology 2024Quote: ... Protein quantity was measured using Bradford method (Sigma-Aldrich). Proteins were stored in aliquots at -80°C until use.
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bioRxiv - Cell Biology 2024Quote: Protein extracts were prepared in RIPA buffer (R0278, Sigma) with protease inhibitor 1X (ab270055 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were blotted onto Polyvinylidene difluoride (PVDF) membranes (Millipore) using a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were wet transferred onto a PVDF membrane (Millipore) and assessed for successful transfer with Ponceau Red (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein expression was induced with 0.2 mM IPTG (Sigma) final concentration ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples were mixed with DTT (#D9779-10G, Sigma) and loading sample buffer (#NP0007 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto PVDF membranes (#IEVH85R, Millipore, USA) using the wet transfer system (#1703930 ...
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bioRxiv - Immunology 2024Quote: ... A protein A-Sepharose column (Sigma #ge17-1279-03) was used for mAb purification ...
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bioRxiv - Microbiology 2024Quote: ... We also employed SYPRO Orange Protein stain (Millipore Sigma), a protein dye ...
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bioRxiv - Neuroscience 2019Quote: ... Synaptic puncta were identified by the co-localization of pre-synaptic Vesicular Acetylcholine Transporter (VAChT) protein and the post-synaptic Shank protein in MAP2 stained neurons using rb anti-VAChT (Sigma Aldrich; 1:1000), ms anti-shank (Neuromab ...
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bioRxiv - Developmental Biology 2020Quote: ... equal amounts of lysates (containing 5 mg of total cellular protein from HeLa cells) were pre-cleared with protein G-agarose beads (Sigma, St Louis, MO). Pre-cleared extracts were incubated with 1 μg rat monoclonal anti-HA (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... and 10-μg protein samples were separated on NuPAGE 4%-12% Bis-Tris protein gels and transferred onto Immobilon-P PVDF membranes (EMD Millipore, Billerica, MA). The membranes were incubated for 1 hour in blocking buffer (Tris-buffered saline with 0.1% Tween and 5% nonfat dry milk ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-HKU1 proteins or lacking the fusion protein (Δ Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland). HAE cultures were washed thrice with 100 μl of 1 × PBS and subsequently inoculated with S-HKU1 or VSV-G pseudoviruses ...
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bioRxiv - Developmental Biology 2020Quote: ... equal amounts of lysate (containing 5 mg of total cellular protein from HeLa cells) were pre-cleared with protein G-agarose beads (Sigma, St Louis, MO). Pre-cleared extracts were incubated with 1 μg rat monoclonal anti-HA (Roche, ...
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bioRxiv - Cancer Biology 2024Quote: PLA was performed to detect protein-protein interactions in MDA-MB-231 cells using Duolink™ In Situ Red Starter Kit (Sigma, cat. DUO92101). All the reagents ...
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bioRxiv - Cell Biology 2023Quote: ... The separation of the hydrophobic and hydrophilic proteins from the pellet was carried out with CelLyticTM MEM Protein Extraction Kit (Sigma-Aldrich, MO, USA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... were coated overnight at 4°C with 20nM rAgApyrase and an unrelated protein amongst the eight candidate proteins (AGAP007393) in carbonate bicarbonate buffer pH 9.6 (Millipore-Sigma, Burlington, MA, USA, #SRE0102). Plates were washed with 0.05% v/v Tween-20 in TBS (25 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... Permeabilized cells were incubated with the specified primary antibodies (mouse monoclonal anti-acetylated tubulin T6793, Sigma-Aldrich; and mouse monoclonal anti-FLAG M2 antibody, F1804, Sigma-Aldrich) for 1 h washed with PBS ...
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bioRxiv - Physiology 2019Quote: ... and incubated for 2 h at room temperature with anti-myosin heavy chain I antibody (MHCI; mouse IgM, Developmental Studies Hybridoma Bank: reference #A4.480) and anti-dystrophin antibody (mouse IgG2b, Sigma Aldrich: reference #D8168) in 5% goat serum diluted in PBS (1:1 PBS dilution) ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were probed with anti-mouse-AID (1/25, eBioscience, 14-5959-82) and anti-mouse-tubulin (1/5000, Sigma-Aldrich, T9026). Then ...
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bioRxiv - Neuroscience 2020Quote: ... one was reacted against anti-calbindin antibody raised in mouse (Swant Inc., Marly, Switzerland) and another against anti-NeuN antibody raised in mouse (EMD Millipore, Germany). The remaining series was reacted with either anti-parvalbumin antibody raised in mouse (Swant Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated for 48 h at 4°C with primary mouse anti-TH antibody (anti-mouse-TH; MAB-318, 1:1000; Millipore, Temecula, CA). The sections were then incubated for 1 h with the appropriate secondary antibody after which sections were washed with PBS and incubated with the required detection kits for development ...
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bioRxiv - Neuroscience 2023Quote: Cytokines from mouse brain homogenates prepared in section 2.7 were measured using Millipore mouse cytokine multiplex kits (EMD Millipore Corporation, Billerica, MA). Calibration curves from recombinant cytokine standards were prepared with threefold dilution steps in the same matrix as the samples ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti-αSMA (Cat: A5228) and mouse monoclonal anti-β-actin (AC-74) (A2228) antibodies were from Sigma (St Louis, MO). Anti-TGFβR1 (Cat ...
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bioRxiv - Neuroscience 2024Quote: ... Incubation with the primary antibodies (anti-aSyn Syn-1 mouse, BD Transduction Laboratories 1:1000; anti-pS129-α-syn Rabbit Abcam Ab51253; mouse anti-β-actin, 1:10.000, Sigma Aldrich) was performed overnight at 4 °C in 5% Albumin Bovine Fraction V (BSA)/TBS-Tween ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-beta actin (A5316, Millipore Sigma, Burlington, MA), mouse anti-tissue transglutaminase 2 (ab2386 ...
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bioRxiv - Neuroscience 2019Quote: ... and mouse anti-NeuN (Millipore: MAB377 at 1:200) primary antibodies for 36-48 hours at 4°C and then secondary antibodies (Alexa Fluor 488 and 594 at 1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... Horseradish peroxidase-conjugated secondary antibodies (anti-mouse; Sigma (UK), anti-rabbit ...
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bioRxiv - Molecular Biology 2020Quote: ... and mouse anti-vinculin (Sigma-Aldrich V9131; 1:1000).