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Citations for Millipore Sigma :
4651 - 4700 of 10000+ citations for Anti CD152 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... for 1h and probed with the following primary antibodies: guinea-pig anti-Oddskipped (1/100, in house) and mouse anti-actin-HRP (1/20 000, Sigma A3854). After incubation with HRP-conjugated anti-guinea-pig secondary antibody (1/10 000 ...
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bioRxiv - Neuroscience 2022Quote: ... before the addition of 17.6 μl anti-KIF1A or anti-PLCB1 antibody and 100 U of Benzonase nuclease (Novagen 70746-3). The mixture was incubated overnight at 4°C on a rotating wheel ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then washed with PBS-T and incubated with anti-rabbit or anti-mouse peroxidase-labeled antibodies (1:10000, Sigma-Aldrich). After extensive washing with PBS-T ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were blocked in 5% horse serum for one hour and incubated with anti-MYC primary antibody (SCBT, 9E10) at a 1:500 dilution or anti-FLAG M2 (Sigma, F1804) at a 1:400 dilution overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Eventually, the blots were probed with indicated primary antibodies: anti-HA (261883, Thermo Fischer) and anti-β-actin (A2228, Sigma-Aldrich) antibodies overnight at 4°C followed by incubation with secondary anti-rabbit (711-035-152 ...
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bioRxiv - Cell Biology 2024Quote: Samples were incubated with secondary antibodies conjugated with PLA probes MINUS and PLUS: the PLA Probe anti-Mouse PLUS and anti-Rabbit Minus (Sigma-Aldrich). The incubation with all antibodies was carried out in a humidified chamber for 1 h at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... After incubation with horse-radish peroxidase (HRP)-conjugated secondary antibodies (goat anti-rabbit-HRP and goat anti-mouse-HRP, Sigma-Aldrich) at room temperature for 1 h ...
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bioRxiv - Biophysics 2023Quote: ... we added a 1:400 solution of primary antibody in blocking buffer (anti-GM130 ab52649 Abcam, anti-tubulin Millipore Sigma T6199). We incubated coverslips with the primary antibody overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... lysates corresponding to 1 OD600 of cells were analyzed by SDS-PAGE and immunoblotted with either anti-c-Myc (9E10, Covance Research) for Myc-tagged proteins or anti-peroxidase soluble complex antibody (Sigma, P2416) for TAP-tagged proteins ...
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bioRxiv - Neuroscience 2023Quote: ... then with primary antibodies (anti-LC3 A/B-12741, Cell Signaling, 1:1500 and anti-alpha tubulin-T6199, Sigma, 1:10000), followed by fluorescent secondary antibody incubation ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed four times with TBST and incubated with secondary antibody (goat-anti-rabbit or donkey-anti-mouse HRP-conjugated,1:5000 in TBST + 10% dry milk, Sigma Aldrich) for 1 hour at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by a 1 hour incubation with an anti-Lkb1 (1:5000, CST, 13031) or anti-NQO1 primary antibody (Nqo1 (1:100, HPA007308, Sigma-Aldrich) in Leica diluent (Leica ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with secondary oligonucleotide-linked antibodies for 1 hr at 37°C (anti-mouse PLUS and anti-goat MINUS, Duolink, Sigma-Aldrich), and processed according to the Duolink PLA fluorescence protocol (Sigma) ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216, Anti-FLAG; 1:2000 dilution, Sigma-Aldrich #F1804 ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-Flag M2 (F1804), anti-Flag M2 Magnetic Beads (10004D), anti-Myc (C3956) antibodies and LPS (O111:B4, L2630) were purchased from Sigma-Aldrich. Anti-FLAG antibody (rabbit source) ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed four times with TBST and incubated with secondary antibody (goat-anti-rabbit or donkey-anti-mouse HRP-conjugated,1:5000 in TBST + 10% dry milk, Sigma Aldrich) for 1 hour at RT ...
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bioRxiv - Cell Biology 2022Quote: ... and then incubated with primary antibodies in blocking buffer [1:400 homemade rabbit anti-RBM20,15 or 1:500 anti-α-actinin (cat# A7811; Sigma-Aldrich)] overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (CST) using the ECL System (Merck Millipore; WBKLS0500). Immunoblot analysis was performed using the LI-COR Odyssey Imaging System.
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bioRxiv - Microbiology 2023Quote: ... anti-p-PKC-δ-T505 (AP0776) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... This was followed by a 1 h incubation at room temperature with anti-mouse or anti-rabbit secondary antibodies conjugated with horseradish peroxidase (HRP; Sigma, A8924). Blots were developed using a Konica SRX101A X-Ray Film Processor.
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with anti-Sulf-1/2 primary antibody (anti-Sulf-1: rabbit polyclonal, Sigma, SAB1410410, anti-Sulf-2: rabbit monoclonal clone 2B4, Sigma, MABC584) overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µL from the supernatant and cell lysates were applied on a nylon membrane and two different antibodies (an HRP-conjugated anti-His tag from Qiagen and an HRP-conjugated anti-strep tag from Sigma-Aldrich) were used on separate blots to detect PR3 ...
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bioRxiv - Microbiology 2023Quote: ... The final dilution of the primary antibody was 1:2,000 for anti-HA tag (Covance) and 1:3000 for anti-β-actin (Millipore-Sigma).and K-Rta (65).
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against target histone modifications were used to detect respective bands (Anti H3K27Me3 #9733 Cell Signaling, Anti H3K9Me #ABE101 Merck Millipore, Anti H3K56Ac #61061 Active Motif ...
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bioRxiv - Biophysics 2024Quote: ... Cells were incubated with 250 μL anti-FITC microbeads (Miltenyi) and 75 μL FITC-conjugated anti-FLAG M2 antibody (Sigma Aldrich) at 4°C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Organisms were stained with anti-MOMP (Meridian Biosciences) primary antibody for all the strains and primary mouse anti-Cpf1 (dCas12) (Sigma-Millipore) for knockdown or complementation samples ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by an overnight incubation step at 4°C with primary antibodies: anti-Smchd1 antibody (1:250; HPA039441; Sigma Aldrich, Zwijndrecht, the Netherlands), anti-Emerin (1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 3 days at 4 °C with antibody cocktail comprising of 1:100 dilution of a Goat polyclonal anti-Chat antibody (EMD Millipore, Cat# AB144P) and 1:200 dilution of GFP-Tag polyclonal antibody conjugated with AlexaFluor 488 ...
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bioRxiv - Biochemistry 2019Quote: ... Them these membranes were subsequently incubated overnight for the western blotting using one of two types of antibodies: monoclonal anti-β-actin antibody (Sigma-Aldrich, USA) or a primary antibody ...
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bioRxiv - Biochemistry 2020Quote: ... The anti-C-ter primary antibody and horse radish peroxidase (HRP)-conjugated secondary antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA). TRIzol™ reagent and Complete™ protease inhibitor cocktail were from Roche (Basel ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was then incubated with the corresponding Alexa Fluor 647-conjugated secondary antibody or Alexa Fluor 555-conjugated anti-mouse IgG1 secondary antibody (Sigma-Aldrich, SAB4600301-50UL) at RT for 1 hour followed by 3 additional wash with TBST buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoblotting for specific proteins was carried out using the following primary antibodies: Mouse monoclonal antibodies against anti-FLAG M2 (Millipore Sigma, Billerica, MA), Jak1 (CST-50996) ...
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bioRxiv - Cell Biology 2020Quote: ... anti-α-adducin phospho S355 antibody (Abmart 20592-1NB-3/C63-S, 1:100, for WB and double transgenics) anti-FLAG antibody (M2, F3165, Sigma-Aldrich 1:100). Myosin Light Chain 2 antibody (ptglab ...
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bioRxiv - Genetics 2022Quote: ... in PBS and incubated with rabbit polyclonal vault antibodies (N2-B15, provided by Leonard Rome, dilution 1:1000) and mouse anti-NeuN antibodies (MAB377, Millipore, dilution 1:1000) in 0.1%NDS/PBS at 4 °C overnight under agitation ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were blocked and incubated with specific antibodies: HA-tagged spike protein was detected with anti-HA antibody (H9658, Sigma-Aldrich, 1:10,000), hACE2 was detected with anti-ACE2 antibody (sc-390851 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation was carried out at 4°C overnight with the following antibodies: mouse anti-Calbindin-D-28K (Sigma, C9848, 1:1000), rabbit anti-vGlut2 (Synaptic Systems ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were washed 6 x 10 mins in PBSTB and incubated with secondary antibody overnight: 1:500 HRP-conjugated Goat anti-rabbit antibody (Millipore Cat# 12-348) followed by washes 6 x 10 with PBSTB and mounted in VECTASHIELD mounting media ...
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bioRxiv - Immunology 2024Quote: Antibodies used for immunofluorescence microscopy were anti-phospho-H2AX (Ser139*) (#05-636, diluted at 1:100) antibody from EMD Millipore (Billerica, MA, USA), anti-AMPK alpha (Phospho Thr172 ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated with primary antibody Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit for detection of TAP-tagged proteins (Sigma-Aldrich, P1291-500UL) 1:1000 dilution in 5 % skimmed milk/PBST overnight at 4 °C followed by washing in 0.1%Tween/TBS ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed 3x for 8min with TBST and incubated with the primary antibody mix containing anti-H2AUb antibody (1:600, 06-678; Millipore, Burlington, MA, USA) and anti-H2A (1:2500 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then treated with primary antibody overnight at 4° C with the following antibodies: neuronal marker anti-NeuN at 1:200 (EMD- Millipore, Temecula, California; AB_2298772); astrocyte marker anti-GFAP at 1:400 (Dako ...
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bioRxiv - Systems Biology 2024Quote: ... Primary antibodies used for western blotting in this study were: mouse anti-4R Tau monoclonal antibody-RD4 (1:800; Millipore sigma# 05-804); mouse anti-3R Tau monoclonal antibody-RD3 (1:800 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... for 1h at room temperature before incubation in primary antibodies (rabbit anti-Synapsin I, ab64581, Abcam; guinea pig anti-GLT-1, ab1783, EMD Millipore; mouse anti-FLAG, F1804, Sigma-Aldrich) at 1:1000 in block for 48h ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... SARS2-57 and SARS2-71 anti-S (VanBlargan et al., 2021) antibodies and HRP-conjugated goat anti-mouse IgG (Sigma, 12-349) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Immunology 2021Quote: ... The membranes were incubated with primary antibodies overnight at 4°C (rabbit anti-PALS-5 diluted 1:1,000 and mouse anti-tubulin (Sigma, catalog number T9026) diluted 1:3000 in blocking buffer) ...
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bioRxiv - Genetics 2021Quote: ... pH 7.4) before being incubated with primary antibodies (Anti-Telo-Collagen Type I, A1, ABT256, Sigma-Aldrich; Anti-Fibronectin-1, F3648, Sigma-Aldrich) followed by anti-rabbit IgG horseradish peroxidase (HRP)-conjugated secondary antibody ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... Organoids were incubated for 2 days with primary antibodies (Anti-GFP at 1:100, A11120, Thermo Fisher Scientific; Anti-Otx2 at 1:100, HPA000633, Sigma-Aldrich) diluted in 1% NDS in PBS-T 0.2% ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was incubated with the primary antibody (rabbit anti-Sarm1, 1:500, ANASPEC 55381; Mouse anti-β-Tubulin, 1:2000, Sigma T5168) at 4°C overnight ...
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bioRxiv - Cell Biology 2019Quote: ... For primary antibodies we used hybridoma supernatant of monoclonal antibody 6E3 Rat anti-CLASP2 (Maffini et al., 2009) at 1:150 and Mouse anti-α-tubulin clone B-512 (Sigma Aldrich) at 1:10000 ...