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Citations for Millipore Sigma :
4601 - 4650 of 10000+ citations for Mouse Anti Hepatitis C Virus NS5a Antibody 1877 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Wells were incubated with serial dilutions of the serum samples and the bound total IgG specific antibodies were detected by Peroxidase-conjugated Goat Anti-Mouse IgG (Sigma-Aldrich). Finally ...
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bioRxiv - Microbiology 2022Quote: ... and incubated with goat anti-mouse antibody conjugated to alkaline phosphatase (Sigma-Aldrich; A3688-1 MI; 1:10,000 1% BSA/PBS) for 2 hr at RT ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.3% Triton for one hour at room temperature prior to incubation with the primary antibody mouse anti-GAD65 (EMD Millipore, MAB3551) at a concentration of 1:100 overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: The following antibodies were used for ChIP against the indicated epitopes: anti-Rpb1 clone 8wG16 (mouse, monoclonal, Millipore # 05-952-I), anti-Rpb1-S2-P clone 3E10 (rat ...
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bioRxiv - Biophysics 2022Quote: ... FLAG- TOP3A-RMI1/2 or FLAG-DNA2 were labeled with QDs pre-incubated with a mouse anti-FLAG antibody (Sigma-Aldrich) on ice for 10 minutes prior to injection ...
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bioRxiv - Biophysics 2022Quote: ... cells were permeabilized with 0.3% Triton X-100 in PBS and then incubated with anti-α-actinin mouse monoclonal antibody (1:100, A7732, Sigma-Aldrich) in CAS-Block reagent (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates collected were run in SDS-PAGE (30 μl loaded) and blotted with the following primary antibodies: mouse anti-Miro1 (WH0055288M1, Sigma-Aldrich) at 1:1,000 ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with Tris buffered saline containing 0.1% Tween-20 and probed again for protein loading control using a 1:10000 dilution of mouse monoclonal anti-actin (plant) primary antibody (Sigma, A0480) and a 1:3000 dilution of goat anti-mouse IgG HRP conjugate (Biorad).
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bioRxiv - Microbiology 2022Quote: ... or a concentration of 50 nM for IMAC-purified DARPins) binding to CagI was performed using a mouse-anti-FLAG M2 monoclonal antibody (dilution 1:5000; Sigma, F1804) as primary and a goat-anti-mouse antibody conjugated to an alkaline phosphatase (dilution 1:10,000 ...
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bioRxiv - Plant Biology 2022Quote: ... Total NUC/GEN polypeptide abundance was detected by probing duplicate blots with a mouse anti-GFP antibody (#11814460001; Sigma Aldrich (Roche), Darmstadt ...
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bioRxiv - Biochemistry 2024Quote: ... PEGylated full-length proteins were transferred to nitrocellulose membrane and probed with mouse anti-HA antibodies (Sigma-Aldrich, H9658, 1:5000) overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... For staining the following primary antibodies were used: For labeling of nerve fibers mouse anti-beta-Tubulin III (1:200, Sigma, T8578); for pre-synaptic terminal staining rabbit anti-Synapsin ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.25% Triton X-100 for 30 min at RT and subsequently incubated in 0.1% Triton X-100 and 1% NS in PB with the following primary antibodies: mouse monoclonal anti-NeuN (1:1000; Millipore, #MAB377), anti-SMI32 (specific for non-phosphorylated neurofilament H ...
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bioRxiv - Neuroscience 2024Quote: ... Triton X-100 and 10% foetal calf serum in PBS] containing primary antibodies including: mouse anti-NeuN (1:200; Millipore, #MAB337); mouse anti-parvalbumin (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
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bioRxiv - Cell Biology 2023Quote: ... Tubulin labeling was carried out with a mouse monoclonal anti-acetylated tubulin antibody (T6793-6-11B-1, Sigma-Aldrich, 1:250) and followed by either a goat anti-mouse IgG Cy3 (115-165-166 ...
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bioRxiv - Genetics 2023Quote: ... Primary antibody was anti-acetylated α-tubulin mouse monoclonal clone 6-11B-1 (1:500 dilution, Sigma, T7451-200, lot #077M4751V). Secondary antibody was Alexa Fluor 488 donkey anti-mouse IgG (1:1,000 dilution ...
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bioRxiv - Systems Biology 2023Quote: ... Thereafter the blocking buffer was discarded and the membranes were incubated with 1:1000 dilutions of mouse anti-FLAG M2 antibody (Sigma-Aldrich) in 1X TBST (overnight ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for immunofluorescence (IF): mouse anti-KHC (clone H2; MAB1614; recognizes primarily KIF5C34) (1:100, EMD Millipore); goat anti-KLC1 (V-17 ...
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bioRxiv - Physiology 2023Quote: ... washed three times with PBS-0.05% tween and followed by incubation with secondary antibodies directed against goat anti-mouse or rabbit HRP conjugated (1:5000, Sigma-Aldrich). The visualization of immunoreactive bands was performed using the enhanced chemiluminescence plus western blotting detection system (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with mouse anti-tyrosine hydroxylase (TH) primary antibody (1:1,000 dilution, Millipore-Sigma, Cat# MAB318, RRID: AB_2201528) and guinea pig anti GFP (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with mouse anti-tyrosine hydroxylase (TH) primary antibody (1:1,000 dilution, Millipore-Sigma, Cat# MAB318, RRID: AB_2201528) and guinea pig anti GFP (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... The grid was subsequently washed with MQ water followed by incubation for 20 min with 10 μl of anti-mouse 10 nm gold-labelled secondary antibody (1:10) (Sigma-Aldrich) and 10 μl of anti-rabbit 5 nm gold-labelled secondary antibody (1:10 ...
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bioRxiv - Cancer Biology 2023Quote: ... The grid was subsequently washed with MQ water followed by incubation for 20 min with 10 μl of anti-mouse 10 nm gold-labeled secondary antibody (1:10) (Sigma-Aldrich). Further ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation for 1 hour at room temperature with a horseradish peroxidase conjugated anti-mouse secondary antibody (Cat# NA931-1ML, Millipore Sigma) diluted 1:1000 in 3% milk ...
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bioRxiv - Biophysics 2023Quote: ... we weakened N-cadherin-mediated cell-cell adhesion of SMCs [49] by using the medium with the addition of mouse monoclonal anti-N-cadherin antibody (10 µg/mL, C3865, Sigma-Aldrich). The SMCs were cultured for 48 h on trough-shaped concavity arrays ...
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bioRxiv - Biochemistry 2023Quote: ... Tubulin was used as the loading control for normalization and probed with Mouse anti-Tubulin antibody (SIGMA, 1 in 3000 dilutions). All the immunoblots of GFP variants were developed ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich (A2179)) in PBST ...
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bioRxiv - Neuroscience 2023Quote: ... sections were re-blocked in blocking buffer for 1 hour at room temperature then incubated in primary antibody solution containing mouse anti-5-HT1A receptor (Millipore Sigma, cat ...
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bioRxiv - Genetics 2023Quote: ... The biotin molecules incorporated into the DNA were visualized by indirect immunofluorescent labeling using 1:60 diluted mouse anti-biotin as a primary antibody (Sigma-Aldrich) applied in 1% BSA/1×PBS/0.1% Triton X-100 for 45 min at RT in a humid chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed with TTBS and incubated for 1 h at room temperature with isotype specific secondary antibodies anti-mouse IgG (1:10,000; Sigma-Aldrich, A4416) and anti-rabbit IgG (1:80,000 ...
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bioRxiv - Genomics 2023Quote: ... and cells were resuspended in 100 μL dig-wash buffer containing 2 mM EDTA and a 1:50 dilution of rabbit anti-mouse antibody (Sigma, M7023) and incubated at RT for 1 hour with slow rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Physiology 2023Quote: ... the secondary antibody was applied by incubating the slides for 1 hour at room temperature with blocking buffer containing Alexa Fluor 647-conjugated goat anti-mouse antibody (1:1000, Sigma, SAB4600354). The slides were then briefly rinsed in PBS-Tw before a 10-minute incubation in PBS-Tw containing 1:2000 SYTOX Orange (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... After washing the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich, A2179) in PBST ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with anti-calbindin-D28k mouse monoclonal antibody (1:2000 dilution by the blocking solution, C9848, Millipore Sigma) at 4 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were electrophoresed on polyacrylamide gels transferred onto PVDF membranes and probed using the following antibodies: mouse anti-beta-Actin (Sigma #A1978), mouse anti-Vinculin (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were washed three times with PBST for 5 minutes each before incubating with anti-mouse secondary antibodies (Sigma-Aldrich, F1804) for at least 60 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were then incubated overnight with primary antibodies against Yes-associated protein (YAP) (mouse monoclonal anti-YAP, 1:400, Sigma-Aldrich) at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of 3x-FLAG tagged S was performed with 1:400 monoclonal ANTI-FLAG® M2 antibody [F3165] produced in mouse (Sigma). 1:50,000 Alexa Fluor® 680 AffiniPure Goat Anti-Mouse IgG [115-625-174] (Jackson ImmunoResearch ...
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bioRxiv - Genetics 2023Quote: ... or with a 1:4000 dilution of a mouse monoclonal anti-α-tubulin antibody (clone B 5-1-2) (Sigma Aldrich). The anti-Crym antibody is specific for an epitope that is located between amino acids 45 and 85 of the Crym protein ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.2% Triton X-100 diluted in PBS for 1 h before incubation with the following antibodies: mouse anti-GFAP (1:1,000; Millipore, Cat. MAB360), rabbit anti-LCN-2 (1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... Bound NS5A antibody was detected after two washes with PBS-T by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse antibody (A4416, Sigma-Aldrich) diluted 1:300 in PBS-T ...
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bioRxiv - Developmental Biology 2023Quote: ... the membrane was incubated for 1h at room temperature with the secondary antibody anti-mouse IgG peroxidase-conjugated (1/500; A5906, Sigma-Aldrich). The revelation was performed using Pierce™ ECL Plus Western Blotting Substrate (32312 ...
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bioRxiv - Plant Biology 2024Quote: ... Probed membrane was washed with TBS-T thrice before incubating with alkaline phosphatase-conjugated anti-mouse secondary antibody (1:10000 dilution, Sigma, USA) or goat anti-rabbit IgG (H+L ...
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bioRxiv - Physiology 2024Quote: ... and proteins were detected by Western blot using monoclonal anti-Flag M2 antibody produced in mouse (1:5000 dilution; Sigma-Aldrich), rabbit anti-FKBP12 antibody (1:1000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... with 200 μl of 15 μg/ml anti-CD63 antibody (Cosmo Bio, SHI-EXO-M02) or mouse IgG2a isotype control (Millipore, MABF1080Z) in PBS containing 0.02% Tween 20 ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were incubated for 2 hours at room temperature with monoclonal anti-β-actin HRP-conjugated mouse antibody (A3854, Sigma-Aldrich). Membranes were developed with Immobilon® Forte Western HRP Substrate (Cat ...
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bioRxiv - Biophysics 2024Quote: ... Immunostaining was performed as previously described [84] using the following antibodies: Mouse anti-Rac1 (Millipore Cat#05-389-25UG, 1:200), Rabbit anti-RhoA (Proteintech Cat#10749-1-AP ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used in this study were as follows: mouse anti-Actub (1:1,000 or 1:2,000, Sigma, 6-11B-1), guinea pig anti-Ick (1:100 ...