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Citations for Millipore Sigma :
4601 - 4650 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... organoids were fixed with ice-cold acetone/methanol (1:1 v/v) and a specific antibody binding was blocked with 5% BSA (w/v, Sigma-Aldrich). Organoids were then incubated with primary antibodies against acetylated α-tubulin (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... and Medium 199 (Earle’s Salts, gibco, 31120022) with a ratio 1:1 and supplemented with 5 % fetal bovine serum (FBS) (Sigma Aldrich, S0615), 50 ng/µl amphotericin B (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed with PBS and replaced with a 1:1 ratio of fresh growth medium and 5 mg/ml of MTT (Sigma Aldrich) stock solution prepared in 1ξ PBS and incubated for 4 hours at 37 °C in 5% CO2 to allow the intracellular reduction of the soluble yellow MTT to insoluble formazan crystals ...
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bioRxiv - Cell Biology 2024Quote: ... NC cells were washed three times in Buffer A and incubated in 1 unit/μl of ligase in diluted Ligase Buffer (1:5, Sigma Aldrich) for 30 minutes at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was blocked with 5% BSA and incubated with primary antibodies against Tuj-1 (dilution 1:10,000, Sigma, Catalog # T8578) and pan-Ago (dilution 1:1200 ...
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bioRxiv - Cell Biology 2020Quote: ... glass-bottomed dishes were first cleaned and activated with 1% v/v (3-Aminopropyl) triethoxysilane (APTES, Sigma-Aldrich) in ethanol ...
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bioRxiv - Neuroscience 2021Quote: ... 3 ml of an enzyme cocktail [1 mg/ml collagenase A (#11088793001, Sigma-Aldrich, St. Louis, MO, USA) and 0.1 mg/ml elastase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... shShank3 injected mice were randomly infused with 2µL (500nL m-1) of vehicle (∼3 % dimethyl sulfoxide (DMSO, Sigma) diluted in aCSF ...
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bioRxiv - Plant Biology 2020Quote: ... low-fat dry milk) or anti-GFP (Sigma-Aldrich, dilution 1:1000 in TBS-T with 3% BSA) primary antibody at 4°C overnight ...
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bioRxiv - Cancer Biology 2019Quote: Cells were treated with blebbistatin (20μM, 3 hr) (Selleckchem, S7099), fasudil (5μM, 24 hr) (Selleckchem, S1573), ML7 (20μM, 1 hr) (Sigma, I2764), ionomycin (10μM ...
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bioRxiv - Plant Biology 2021Quote: ... 250 μM or 1 mM L-glutamic acid monosodium salt monohydrate (Sigma Aldrich, cat No. 6106-04-3). Plants were analyzed in single blinded-experiments ...
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bioRxiv - Biochemistry 2021Quote: ... 3 nM of detergent-solubilized or nanodisc-reconstituted complex I and either 1 mM FeCy (Sigma Aldrich BVBA) or 100 μM Q1 (Sigma Aldrich BVBA ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... and construct expression was induced for 3 days using media above with 1 µg/mL tetracycline (Sigma Aldrich) added ...
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bioRxiv - Biochemistry 2020Quote: ... Japan) and 1:3 mixture of PVA and polyvinylpyrrolidone (PVP: average MW, 40,000; Sigma-Aldrich, St. Louis, MO), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was exposed to donkey anti-mouse IgG conjugated to Cy-3 in PB (1:80; EMD Millipore) for 1 h for visualization of Hu ...
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bioRxiv - Neuroscience 2020Quote: ... counterstained with DAPI (diluted 1:1000 in PBS) for 3 minutes at RT and mounted with Fluoromont (Sigma).
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bioRxiv - Microbiology 2021Quote: ... The resulting liquid was aliquoted into 4 phase lock tubes (Quanta Biosciences) and extracted 3 times with an equal volume of phenol:chloroform:isoamylalcohol (25:24:1, Sigma). A final extraction with an equal volume of chloroform (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... followed by staining of cell nuclei for 3 min at RT with 1 μg/ml Hoechst 33342 (Sigma) in PBS−/− ...
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bioRxiv - Microbiology 2020Quote: ... blocked for 1 h in PBS with 3% normal goat serum and 0.5% bovine serum albumin (BSA; Sigma), and incubated for 1 h with anti-VP1 rabbit serum (1:200 ...
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bioRxiv - Cell Biology 2021Quote: The gRNA complex was prepared by mixing 1 μl of tensin 3 crRNA (100 μM; AGUC-CGCUCCCGCUCAUAG, SIGMA) and 1 μl of trRNA (100 μM ...
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bioRxiv - Cell Biology 2021Quote: ... (3) 30 μL casein wash buffer (30 mM DTT, 1 mg/mL filtered casein (Sigma-Aldrich, C5890-500G) in BRB80 pH 6.8) ...
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bioRxiv - Cell Biology 2020Quote: ... for 3 hr to induce mCherry–FokI expression and 1 mg/ml doxycycline hyclate (D9891–G, Sigma–Aldrich) for an additional 3 hr to induce reporter gene transcription ...
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bioRxiv - Biochemistry 2022Quote: ... 0.02% Triton X-100 and blocked for 1 hour in a 3% Bovine Serum Albumin (BSA, Sigma Aldrich), 0.02% Triton X-100 blocking solution ...
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bioRxiv - Bioengineering 2022Quote: ... 3 the cell culture media was replaced 1-2 hours before imaging with RPMI media (Sigma-Aldric R8755) supplemented with B27 ...
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bioRxiv - Bioengineering 2022Quote: ... After blocking for 1 h by adding a drop of 3 % bovine serum albumin (A9418, Sigma-Aldrich, USA) onto exposed tissue ...
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bioRxiv - Developmental Biology 2020Quote: ... for 3 minutes and incubated at room temperature for 30 minutes in 1% Alcian Blue 8GX (Sigma-Aldrich) in 3% acetic acid solution ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: Mice were fasted for 3 h and injected with Triton WR-1339 (1 mg/g BW, Sigma-Aldrich) via the tail veins ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately1-2 μL of endotoxin-free DNA (1-3 mg/ml) diluted in PBS/0.025% Fast Green (SIGMA) was injected into the lateral ventricles of the forebrain using heat-pulled glass micropipettes (Drummond) ...
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bioRxiv - Microbiology 2022Quote: ... LAM was resuspended at 1 mg/mL in sterile nanopure water (Direct-Q 3 UV-R, Millipore Sigma). 60-μL aliquots were stored in low-retention tubes at –80 °C and thawed immediately prior to use ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected using a 3:1 polyethylenimine (PEI [branched average Mw ~25,000 Da; Sigma-Aldrich]) to DNA ratio (30:10 μg ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were then washed in PBTD for 3×10 min and 1mg/ml DAPI (1:250, Sigma-Aldrich) was added and incubated for 10 minutes between the first and second washes ...
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bioRxiv - Developmental Biology 2020Quote: ... containing pre-equilibrated preimplantation embryo culture media supplemented with 1 µM PDO325901 and 3 µM CHIR99021 (Sigma-Aldrich). Embryos were grown one more day in NDiff 227 media (Takara ...
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bioRxiv - Microbiology 2019Quote: ... multi-tube mixer for 3 min before adding one volume of phenol:chloroform:isoamyl alcohol (25:24:1) (Sigma-Aldrich). Samples were mixed vigorously ...
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bioRxiv - Microbiology 2020Quote: ... and 200 μM of a non-selective family-wide phosphodiesterase inhibitor 3-Isobutyl-1-methylxanthine (IBMX; Sigma, #I5879) in a mix of DMEM/F-12 medium (50/50 ...
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bioRxiv - Neuroscience 2020Quote: To label endogenous RNAs neurons were washed once with cold PBS with 3 μg.ml-1 digitonin (Sigma Aldrich), once with 50% methanol in PBS and fixed in cold 100% methanol for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Appropriate fractions were concentrated to 3-0.7 mg mL−1 using 30,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Cell Biology 2020Quote: DNA was precipitated with 1 volume of isopropanol and 0.1 Volume of 3 M sodium acetate (pH 5.2, Sigma) at −80°C for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Total RNA of the macrophages was extracted by the phenol/1-bromo-3-chloropropane method (Sigma-Aldrich, UK) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μM 1-[2-[[(Diphenylmethylene)imino]oxy]ethyl]-1,2,5,6-tetrahydro-3-pyridinecarboxylic acid hydrochloride hydrochloride (NO711) (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was resuspended and incubated for 30 min at 4 °C in buffer B (10 mM Tris pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 0.5% IGEPAL CA-630, 10% Glycerol, 1 mM DTT, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail) ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected using a 3:1 polyethylenimine (PEI [branched average Mw ∼25,000 Da; Sigma-Aldrich]) to DNA ratio (30:10 μg ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Cell Biology 2022Quote: ... or 10nM GIP in stimulation buffer (1x Hanks Buffered Saline Solution, 0.1% BSA, 0.1% 3-isobutyl-1-methylxanthine (IBMX) (all Sigma), 0.5mM HEPES (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: D1R agonist 2,3,4,5,-tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine hydrochloride (SKF38393; Sigma-Aldrich, St Louis, MO) was dissolved in sterile saline at a concentration of 0.5 μg/μL ...
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bioRxiv - Biochemistry 2024Quote: ... SidK(1-278)-3×FLAG (∼0.85 mg) was bound to 1.5 mL of M2-FLAG affinity gel resin (Sigma) and flow through was recirculated onto the column 6-7 times ...
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bioRxiv - Cell Biology 2024Quote: ... in 3% (w/v) BSA were added for 1 hour before addition of 300 nM DAPI (Sigma Aldrich) and ProLong® Gold Antifade (Cell Signaling Technology) ...
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bioRxiv - Biochemistry 2023Quote: ... and phosphatase inhibitors (prepared in-house according to Phosphatase inhibitor cocktail 1, 2 and 3 from Sigma-Aldrich)) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed with 1× PBS and blocked in 3% BSA with 0.1% Triton-X 100 (Thermo-Fisher) and 0.1% Tween (Sigma) in PBS for one hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The final supernatant was concentrated to > 1 mg/ml using a 3 kDa centrifugal filter unit (Millipore Sigma) and frozen in aliquots stored at −80°C ...