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Citations for Millipore Sigma :
4501 - 4550 of 10000+ citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... containing 10% heat-inactivated pooled human sera and 5 μg/mL gentamicin (Sigma, USA). Cultures were maintained at 37°C in a gas mixture composed of 5% O2 ...
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bioRxiv - Immunology 2023Quote: ... encoding a gRNA for human AAVS1 targeting (GGAAGAGAGTAGGTCGAAG) using GeneJuice Transfection Reagent (Sigma-Aldrich). Transfected cells were selected with either 0.5μg/mL puromycin or 100μg/mL neomycin ...
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bioRxiv - Bioengineering 2023Quote: ... human skeletal muscle biopsies were minced and incubated with collagenase (Sigma, 630 U/mL) and dispase (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal antibody against human Integrin alpha-5 (1:100 for immunofluorescence; EMD Millipore; catalogue number MABT201 ...
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bioRxiv - Cancer Biology 2023Quote: ... human PBMCs were labelled with the fluorescent membrane marker PKH26 (Sigma-Aldrich, MINI26-1KT). For the in vivo killing assay ...
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Dual inhibition of coronavirus Mpro and PLpro enzymes by phenothiazines and their antiviral activitybioRxiv - Microbiology 2023Quote: ... Coverslips were blocked and permeabilized in staining buffer [1% human serum (Sigma Aldrich, 4552) heat-inactivated at 56 °C for 1 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... or 2 µg of MATR3 siRNA (MISSION esiRNA human MATR3 Sigma-Aldrich EHU113801-50UG) using Lipofectamine 3000 Transfection Reagent (ThermoFisher Scientific L3000015 ...
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bioRxiv - Microbiology 2023Quote: ... Purified human IgG and IgA were used as standards (Sigma, St. Louis, MO, USA). Tear samples were tested by 4-fold serial dilution ...
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bioRxiv - Cell Biology 2023Quote: ... we used MISSION esiRNA for MDM4 (ESIRNA HUMAN MDMX, Cat. # EHU005381-20UG; Sigma-Aldrich) and MISSION esiRNA for Renilla Luciferase as a control (ESIRNA RLUC Cat ...
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bioRxiv - Biochemistry 2023Quote: Purified human NUDT15 (20 mg/mL) was preincubated with 10 mM GPP (Sigma-Aldrich) for 2 hours at 20 °C ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4 °C with purified collagen I from human placenta (Sigma), gelatin from bovine skin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4 °C with purified collagen I from human placenta (Sigma), gelatin from bovine skin (Sigma ...
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A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo developmentbioRxiv - Cell Biology 2024Quote: ... females were injected with 5 I.U of human chorionic gonadotropin (hCG) (Sigma-Aldrich #CG5) to induce ovulation ...
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bioRxiv - Bioengineering 2024Quote: ... Human hepatic stellate cell line LX-2 were acquired from Sigma (cat. no. SCC064), and were cultured in DMEM-2 (Thermo Fisher ...
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bioRxiv - Genetics 2024Quote: ... Spermatozoa were capacitated in Human Tubal Fluid medium (HTF) (Merck Millipore; MR-070-D) supplemented with 10 mg/ml Albumin (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Human microvascular endothelial cells (HMEC-1) were cultured in MCDB-131 medium (Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Cancer Biology 2024Quote: ... The 22Rv1 human prostate cancer cell line was grown in RPMI-1640 (Sigma Aldrich) and supplemented with 1% L-Glutamine (Gibco ...
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bioRxiv - Genomics 2024Quote: All human mammary epithelial cell lines MCF-10A TP53+/+ and TP53-/- (Sigma-Millipore clls1049) were cultured in 1:1 Dulbecco’s Modified Eagle’s Medium ...
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bioRxiv - Cell Biology 2024Quote: ... The chips were then quickly coated with 100 ug/mL human fibronectin (Sigma #SLCL0793) diluted in PBS and injected using a pipette tip ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... Patu8988S and MiaPaCa-2 human pancreatic cancer cells were cultured in RPMI (Sigma, #R1383) or DMEM (Gibco ...
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bioRxiv - Immunology 2024Quote: ... Human CD34+ cells were isolated from FL by density gradient centrifugation (Histopaque-1077, Sigma) followed by positive immunomagnetic selection using anti-human CD34 microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: Human cell lines: T47D (HTB-133, ATCC) were cultured in RPMI-1640 (R8758, Sigma) and supplemented with 0.2 unit/ml bovine insulin (I0516-5ML ...
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bioRxiv - Genomics 2024Quote: All human mammary epithelial cell lines MCF-10A TP53+/+ and TP53-/- (Sigma-Millipore clls1049) were cultured in 1:1 Dulbecco’s Modified Eagle’s Medium ...
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bioRxiv - Immunology 2024Quote: The MILLIPLEX MAP Human CD8⁺ T-Cell Magnetic Bead Panel (Merck Millipore Darmstadt, Germany) was used to quantify GM-CSF ...
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bioRxiv - Cell Biology 2024Quote: ... NSCs were validated for expression of mouse anti-human Nestin (1:2,000; Millipore; MAB5326), rabbit-anti Sox2 (1:400 ...
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bioRxiv - Developmental Biology 2024Quote: ... followed 48 hours later by 5 IU of human chorionic gonadotropin (hCG; Sigma-Aldrich). Alternatively ...
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bioRxiv - Plant Biology 2020Quote: 15N-accumulation assay after 15N-nitrate or 15N-ammonium labeling was performed with 15N-labeled KNO3 (99 atom % 15N, Sigma-Aldrich, no. 335134) or 15N-labeled NH4Cl (98 atom % 15N ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were captured on pre-labeled 1×3 mm glass slides coated with 10% poly-L-lysine (Sigma Aldrich, St. Louis, MO, USA) and maintained at 37° during sectioning ...
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bioRxiv - Biochemistry 2020Quote: ... cells were grown with light labeled amino acids with both LPS (1 µg/ml) and curcumin (10 µM, Sigma-Aldrich, St. Louis, MO) and heavy labeled amino acids with LPS (1 µg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with the indicated concentrations of fluorescently labeled LDL or VLDL in serum-free media containing 0.1% BSA (Sigma-Aldrich, St. Louis, MO) for 4 h at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Lipid fluorophore 1,2 – Dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto 647 (Atto 647 DOPE) was purchased from Sigma Aldrich (St. Louis, MO). HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were transferred to 6 well culture dishes and rehydrated with MilliQ water and labeled in H2O for 5 min with Dapi (Sigma-Aldrich, Cat # 9542) 1:10,000 dilution of 1 mg/ml aqueous stock and Syber Gold 1:20,000 dilution of DMSO stock (ThermoFisher Cat # S11494) ...
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bioRxiv - Plant Biology 2022Quote: ... Full-length FIN4 mRNA in PCRII-TOPO vector was used to synthesize sense and anti-sense probes using a DIG-labeled probe synthesizing kit (Millipore-Sigma, catalog number 11175025910) with T7 RNA polymerase for sense probe and SP6 RNA polymerase for antisense probe synthesis with 1μg plasmid as template ...
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bioRxiv - Bioengineering 2019Quote: To measure the permeability of the cholangiocyte monolayers in the device, fluorescent dextran (70 kDa, 10 kDa and 4 kDa, labeled with fluorescein isothiocyanate (FITC) (Sigma, St. Louis, MO)) in phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2021Quote: Brains of male and female mice expressing EYFP in MC3R neurons were collected and processed as describe above and neurons expressing TH were labeled using standard immunohistochemical (IHC) techniques (as described above for c-fos) using mouse anti-TH antibodies (Catalog # MAB318, Millipore, Inc., Billerica, MA) diluted 1:1500 followed by Alexa Fluor 594 conjugated goat anti-mouse antibodies (Catalog # A-11032 ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 mL of a UPLC-grade water:methanol (3:1, v/v) solution with 1:500 diluted 13C-and 15N-labeled amino acids standard mix (Sigma-Aldrich, Merck, Darmstadt, Germany) was added to the tubes ...
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bioRxiv - Biochemistry 2023Quote: ... cells were labeled and maintained in SILAC DMEM medium that was supplemented with 1mM 13C6 N2-L-lysine (Cat#608041, Sigma- Aldrich, Shanghai, China), 10% dialyzed fetal bovine serum ...
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bioRxiv - Biophysics 2023Quote: ... Lipid fluorophore 1,2 – Dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto 647 (Atto 647 DOPE) was purchased from Sigma Aldrich (St. Louis, MO). Thapsigargin was used at 2 μM (AC328570010 ...
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bioRxiv - Neuroscience 2024Quote: Neuronal processes in octopus arm were labeled with mouse monoclonal antibody 6–11B-1 (1:500 dilution of ascites fluid; Sigma-Aldrich, Cat#: T6793) and mouse monoclonal antibody SMI-31 (1:500 dilution ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... and precleared using protein A agarose beads (25%, diluted in ChIP dilution buffer; Protein A Agarose/Salmon Sperm DNA, Millipore, 16-157) and incubated overnight at 4°C (final buffer composition during antibody incubation was 0.1% SDS ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined using a Bradford protein assay kit (Thermo-Fisher, MA, USA) with bovine serum albumin (Sigma-Aldrich, MO, USA) as the standard ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was determined using BCA™ protein assay (Pierce, Rockford, IL, USA) using BSA (Sigma- Aldrich, St. Louis, MO, USA) as a standard ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The soluble and insoluble protein fractions were extracted using a CelLytic™ MEM Protein Extraction Kit (Sigma–Aldrich, St. Louis, MO, USA) and a CelLytic™ NuCLEAR™ Extraction Kit (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: Approximately equal amounts of GST fusion proteins and alanine-mutated GST fusion proteins were separated by 12% SDS-PAGE and transferred to PVDF transfer membranes (Millipore, MA, USA). The membranes were blocked with 5% skimmed milk powder for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2022Quote: The expression vector pGEX-6P-1-GST-FLAG containing the relevant fusion protein and fluorescence protein was transformed into Rosetta™ 2 (pLysS) host strains (Novagen; 71403). A single colony was inoculated in 5.5 mL LB broth (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were performed on both total proteins and proteins eluted from the anti-GFP affinity beads using anti-FLAG (Sigma-Aldrich, F31651), anti-GFP (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... the expression levels of recombinant B56 subunit and PME-1 were monitored by western blot using antibodies that specifically recognize HA-tag (Sigma, 2CA5, Cat#: 116666060011, 1:1000) and PME-1(Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... Prior to labelling the apoplastic fluid was incubated for 30 min with different concentrations of recombinant LpCys1 or chicken egg white cystatin (CEWC, Sigma-Aldrich, St. louis, Mississippi, USA) and Tris-HCl (24 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... the chamber was rinsed three times with T50 to remove non-specific interactions and a protocatechuic acid/recombinant protocatechuate-3,4-dioxygenase (Sigma Aldrich and Oriental Yeast Co., respectfully) (PCA/rPCD ...