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Citations for Millipore Sigma :
4451 - 4500 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and then the proteins were transferred to the PVDF membranes (Millipore). After being blocked by the 3% BSA (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were transferred to Immobilon-P transfer membranes (Sigma-Aldrich) using the Trans-Blot® TurboTM Transfer System (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were extracted as whole cell lysate using trichloroacetic acid (Sigma) and glass beads as described before87.
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bioRxiv - Developmental Biology 2022Quote: Proteins were transferred to 0.45 μm Immobilon-P PVDF membrane (Millipore) in transfer buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... For conditional induction of protein expression 1μg/ml of doxycycline (Sigma) was added to the culture media and incubated for 16-24h.
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bioRxiv - Cell Biology 2022Quote: ... Transfer of protein on activated PVDF membranes (EMD-Millipore, cat#IPVH07850) were performed overnight at 4°C at 50mA ...
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bioRxiv - Cell Biology 2022Quote: ... Protein quantification was performed by the bicinchoninic acid method (Sigma-Aldrich) for normalization of lipolysis data.
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bioRxiv - Cancer Biology 2023Quote: Protein extracts were prepared by lysing cells in RIPA buffer (Sigma) supplemented with a phosSTOP tablet (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... The protein was concentrated using an Amicon centrifugal concentrator (EMD Millipore) as necessary ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein supernatants were collected and quantified by Bradford assay (Sigma Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Resolved proteins were transferred overnight into a PVDF membrane (0.22µm, Millipore) followed by 30 min incubation in blocking buffer (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to a polyvinylidene fluoride membrane (Merck Millipore), followed by blocking in 2% BSA containing 50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were then filtered through a 10kDa protein filter (Millipore) by centrifugation at 12,000g ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were lysed with Cytobuster™ Protein Extraction Reagant (Millipore) supplemented with HALT protease inhibitor cocktail ...
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bioRxiv - Immunology 2023Quote: PureProteome Protein A magnetic beads were obtained from EMD Millipore (LSKMAGA10). The RPMI-1640 media with L-glutamine ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against the following proteins were used: FLAG (Sigma Aldrich F1804), Streptavidin-Alexa Fluor 488 (Life S32354) ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were separated by 8% resolving gel containing trichloroethanol (TCE; Sigma) to visualize proteins (Ladner et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were concentrated using an ultracel-100 membrane filter unit (Millipore) and then the concentration of NaCl was adjusted to 100 mM ...
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bioRxiv - Biochemistry 2023Quote: ... filtered through a 0.45 mM low protein-binding PVDF filter (Millipore) and applied to a 1 mL StrepTrap HP column pre-equilibrated in Strep-start buffer (Cytiva) ...
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bioRxiv - Physiology 2022Quote: ... proteins were transferred to PVDF membranes (Merck Millipore, IEVH00005, Tokyo, Japan), blocked with 2.5% non-fat milk in PBS containing 0.1% Tween 20 for 1 h ...
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bioRxiv - Physiology 2023Quote: ... The lysosomal protein degradation inhibitor chloroquine (ChQ; 100 μM; Sigma-Aldrich) was added to the cell culture medium 30 minutes prior to ghrelin treatment for 6 hours.
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated in a 50,000 MWCO Amicon Concentrator (Millipore) to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare ...
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bioRxiv - Bioengineering 2023Quote: ... Then protein signal was detected using Immobilon Western HRP substrate (Millipore) and Fusion FX6 Imaging System (Vilber ...
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bioRxiv - Microbiology 2023Quote: ... Then protein signal was detected using Immobilon Western HRP substrate (Millipore) and Fusion FX6 Imaging System (Vilber ...
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bioRxiv - Cell Biology 2023Quote: BIX (BiP protein inducer X) was obtained from Sigma (catalog #: SML1073). Thapsigargin (Tg ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein extraction was carried out in RIPA buffer (Millipore, USA) for infected cells and in aTris-HCl 20 mM pH 8,1 ...
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bioRxiv - Microbiology 2023Quote: ... 30 μl of protein G agarose beads (EMD Millipore, Temecula, CA) was added ...
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bioRxiv - Physiology 2023Quote: ... Protein was visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore; #WBKLS0500) and imaged on a ChemiDoc (BioRad) ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were detected using the Immobilon Western Chemiluminescent HRP Substrate (Millipore) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Protein gels were stained overnight with colloidal Coomassie Brilliant Blue (Sigma). Each lane was excised into 9 sections that were destained and in-gel digested overnight using trypsin (sequencing grade ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitinated proteins were detected using an anti-FLAG antibody (F7425, Sigma), HA-TAP-Gb1 and endogenous Gb1-4 were detected with anti-HA (16B112 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected by a monoclonal anti-FLAG antibody (Sigma, USA) or a polyclonal anti-GFP antibody (GenScript ...
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bioRxiv - Cell Biology 2023Quote: ... Protein bands were detected with Immobilon Western HRP Substrate (Merck Millipore) and images captured with a CCD camera and the Bio-Rad ChemiDoc MP System (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: The lysate was pre-cleared with ∼20µL Protein G agarose (Sigma) per 1mL of lysate at 4°C for 30min ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Cell cycle control protein 50A (anti-CDC50A/TMEM30A, Sigma, AV47410), anti-Microtubule-associated protein 1 light chain B (anti-MAPLC3/LC3 ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were visualized using chemiluminescence (Immobilon ECL western HRP substrate, Millipore). Densitometric analysis was carried out using the Image J software ...
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bioRxiv - Neuroscience 2023Quote: ... anti-prosurfactant protein C (proSP-C, Millipore Sigma AB3786, RRID: AB_91588) was used at 1:4,000 with microwave antigen retrieval as described above ...
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bioRxiv - Immunology 2023Quote: ... injection of 40 μg of OVA protein (Grade V; Sigma-Aldrich) adsorbed to 2 mg of aluminum hydroxide (alum ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration was then measured using a Bradford assay (#B6916, Sigma), after which the proteins were diluted in Promega buffer (50 mM NaCl ...
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bioRxiv - Immunology 2023Quote: ... injection of 30 μg of OVA protein (Grade V; Sigma-Aldrich) adsorbed to 2 mg of aluminum hydroxide (alum ...
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bioRxiv - Microbiology 2023Quote: ... Target proteins were visualized using Immobilon Western Chemiluminescent HRP substrate (Millipore).
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was determined by the Bradford method (Sigma-Aldrich, B6916). 50 μL magnetic beads (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-pro-surfactant protein C (SFTPC, 1:1000, AB3786, Millipore), goat anti-SOX9 (SOX9 ...
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bioRxiv - Biophysics 2023Quote: ... Purified proteins were concentrated using Amicon Ultra 3K MWCO filters (Millipore) in storage buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were blotted onto Immobilon-P PVDF membrane (0.2 µm, Millipore) using a Trans-Blot Semi-Dry transfer Cell (BioRad ...
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bioRxiv - Biophysics 2023Quote: ... Protein expression was performed in Bl21 (DE3) pLys competent cells (Novagen) using an L.B ...
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bioRxiv - Biochemistry 2023Quote: ... 25 µg of protein was reduced with 2 mM dithiothreitol (Sigma) for 60 minutes at 55 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were also lysed in BugBuster Protein Extraction buffer (EMD Millipore) for 30 min instead of sonication.
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bioRxiv - Cell Biology 2023Quote: ... Cenexin rabbit antibody (Protein tech) and centrin mouse antibody (EMD Millipore) diluted 1:1000 for centrin and 1:200 for other centrosomal proteins in 1% BSA in PBS for 1.5 hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred from the gel onto PVDF membranes (EMD Millipore) using standard methods ...