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Citations for Millipore Sigma :
4451 - 4500 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Protein concentration was calculated following Bradford assay (Bradford reagent, Sigma-Aldrich). A 1:50 mixture of β-mercaptoethanol (Acros Organics ...
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bioRxiv - Neuroscience 2024Quote: ... and samples diluted in Assay buffer (1 % BSA (#A9647, Sigma-Aldrich), 0.05 % Tween20 in DPBS) ...
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bioRxiv - Molecular Biology 2024Quote: ... then assays performed as described for MC3R with somatostatin-14 (Sigma) added as the agonist ...
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bioRxiv - Neuroscience 2024Quote: ... The ELISA assays were developed using Super Slow ELISA TMB (Sigma) with absorbance read on a BioTek Epoch plate reader at 650 nm ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined by a microplate Bradford assay (Sigma-Aldrich). 1 mg aliquot of each sample was taken for further digestion and phosphopeptide enrichment.
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bioRxiv - Cancer Biology 2024Quote: MTT assays were conducted following the manufacturer’s instructions (Sigma Aldrich, Belgium). Three thousand cells were seeded in each well of a 96-well plate and incubated for 24 hours in 5% CO2 incubator at 37°C overnight ...
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bioRxiv - Bioengineering 2024Quote: ... For the TCR inhibition assay using dasatinib (Sigma Aldrich SML2589-50MG), activated T cells were pre-treated with 50 nM dasatinib for 30 min prior to APN transfection35 ...
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bioRxiv - Cancer Biology 2024Quote: ... Simvastatin and Rosuvastatin used in all assays were obtained from Sigma (Simvastatin Cat No ...
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bioRxiv - Cell Biology 2024Quote: Proximity ligation assay (PLA) was performed using DuoLink PLA (Millipore Sigma) following the recommended protocol ...
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bioRxiv - Plant Biology 2020Quote: ... The medium containing root exudates was filtered through Omnipore membrane filters (Millipore, Darmstadt, Germany). The medium was passed through a Sep-pak C18 Plus short cartridge (Waters ...
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bioRxiv - Plant Biology 2020Quote: ... total membrane fractions were dissolved in 2× TruPAGE™ LDS Sample Buffer (Sigma-Aldrich) at 70 °C for 5 minutes (in a minimal of 80μl) ...
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bioRxiv - Cancer Biology 2021Quote: ... fractionated by SDS polyacrylamide gels and transferred to PVDF membranes (Millipore Sigma, MA, USA). After blocking with 5% non-fat dry milk (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were resolved using SDS-PAGE and transferred onto Immobilon-FL PVDF membranes (Millipore). Membranes were blocked using Odyssey blocking buffer (Li-Cor ...
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bioRxiv - Cancer Biology 2021Quote: ... and thereafter protein transferred onto 45 µm Polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Western blotting analysis was performed by using specific primary and secondary antibodies and signals visualized with chemiluminescence ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysates were resolved by SDS-PAGE and transferred onto a PVDF membrane (Millipore). Membranes were blocked with 2% BSA in TBS tween20 0.1% and incubated in the presence of the primary and then secondary antibodies ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... multiScreen-HA sterile 0.45 µm surfactant-free mixed cellulose ester membrane filter plate (Millipore) wells were coated with 50 µl of 25 µg/mL unlabeled goat anti-mouse IgG (SouthernBiotech) ...
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bioRxiv - Cell Biology 2020Quote: ... Separated proteins were transferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Millipore, Cork, Ireland), which were blocked with a 2:1 ratio of Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated in polyacrylamide gel and then transferred to polyvinylidene difluoride membranes (Millipore). These membranes were incubated with primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... coated 6.5 mm 0.4 μM Corning® Transwell® porous polyester membranes (Sigma CLS3470). Cultures were supplemented with PneumaCult Ex-Plus Expansion media (Stemcell 05040 ...
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bioRxiv - Microbiology 2020Quote: ... for which a cellulose membrane (10 kDa) was used in the Amicon Ultracel (Millipore) ultrafiltration system at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The SCN slices were dissected out and transferred onto a PTFE membrane insert (Millipore) in 35-mm culture dishes with 1.2 ml of DMEM (D5030 ...
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bioRxiv - Cell Biology 2020Quote: ... PVDF membranes were blocked in 5% milk/TBS 10% glycerol + 0.05% tween20 (Sigma-Aldrich) (TBS/t ...
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bioRxiv - Developmental Biology 2020Quote: ... The same membrane was stripped and incubated with an antibody against ACTB (Millipore Sigma) as loading control of protein samples.
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bioRxiv - Neuroscience 2021Quote: ... PVDF membranes were submerged for 10 min in 0.008% Direct Blue 71 (Sigma-Aldrich) in 40% ethanol and 10% acetic acid ...
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bioRxiv - Molecular Biology 2020Quote: ... separated in 10% SDS-polyacrylamide gel and transferred on Immobilon-P membrane (Millipore #IPVH00010).
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bioRxiv - Molecular Biology 2020Quote: ... Bottom half of epidermal sample membranes were incubated with rabbit anti-RPL36 (Sigma; HPA047153) and top halves with rabbit anti-VCL (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was further hybridized at 68°C for overnight using PerfectHyb PLUS (Sigma) with a P32UTP-labeled probe that has a complementary sequence against Per2AS (1-1812 nt ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were probed with the required primary antibodies: rabbit anti FLAG (F7425, Sigma Aldrich), Rat anti-HA (11867423001 ...
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bioRxiv - Molecular Biology 2020Quote: ... Immobilon Western chemiluminescent HRP substrate and nitrocellulose membranes were from Millipore (Burlington, MA, US). The QIAquick PCR purification kit was from Qiagen (Hilden ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were blocked with 5% non-fat dried milk plus 0.1% Tween (Sigma, P1379) in TBS (TBST ...
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bioRxiv - Developmental Biology 2022Quote: ... and separated proteins were transfer to polyvinylidene fluoride (PVDF) membranes (EMD Millipore, Fisher Scientific). Total lane protein was then determined via Ponceau stain (0.1% Ponceau-S in 5% acetic acid ...
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bioRxiv - Microbiology 2020Quote: ... Detection was carried out by incubating membranes with ECL Prime Western Blotting System (Sigma) and GE healthcare Amersham Hyperfilm ECL (GE) ...
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bioRxiv - Genetics 2020Quote: ... the membranes were incubated with secondary antibody (Millipore 3136001, Rabbit IgG, 1:3000 dilution). The blots were reacted with chemiluminescent HRP substrate (Millipore WBKLS0500 ...
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bioRxiv - Microbiology 2020Quote: ... 100 mL water was filtered through microfilters (Millipore 0.45 μm membrane, diameter 47 mm), and the membranes were transferred into 10 mL Z-broth ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were probed overnight at 4°C with anti-SETD2 (Sigma, catalogue no. HPA042451) and anti-beta-actin (Cell Signaling Technology ...
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bioRxiv - Pathology 2021Quote: ... proteins were transferred from the gel onto to a polyvinylidene fluoride membrane (Merck Millipore) via electroblotting at 120 V for 60 minutes in transfer buffer containing 20% methanol ...
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bioRxiv - Neuroscience 2020Quote: ... gels were transferred for 2 hours on ice at 100V onto PVDF membranes (Millipore), blocked for 1 hour at room temperature in 5% milk in TBST (Tris Buffered Saline with 0.02% Tween-20 ...
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bioRxiv - Neuroscience 2021Quote: ... then positioned on 0.45 µm Durapore membranes on Millicell culture inserts (Millipore, FHLC01300, PICM0RG50) in 6-well plates ...
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bioRxiv - Cancer Biology 2020Quote: ... cell lysates were subjected to SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Millipore). The membranes were immunoblotted with the indicated antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were incubated in blocking solution containing 5% skim milk (Sigma-Aldrich, MO, USA) in tris-buffered saline (pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... The exosomes present in the supernatant were filtered through a 0.22-μm membrane (Millipore) to a final volume of 1 ml and then further centrifuged (120000 g ...
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bioRxiv - Biochemistry 2020Quote: ... in 1X NuPAGE buffer followed by transfer to Immobilon-FL polyvinylidene difluoride membrane (Millipore). Membrane was blocked using Intercept Blocking Buffer (LI-COR) ...
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bioRxiv - Biochemistry 2020Quote: ... Each portion was concentrated with 30 kDa cut-off membrane (SIGMA-ALDRICH #Z677892-24EA) to 0.9 ml and 2.5 ml ...
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bioRxiv - Bioengineering 2020Quote: ... The membranes of L929 murine fibroblasts and RAW macrophages were labeled with PKH26 (Sigma, PKH26 Red Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling ...
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bioRxiv - Biochemistry 2020Quote: ... The gels were subsequently blotted to an Immobilon®-P PVDF Membrane (Millipore, MA). After blocking with 5% skim milk solution ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were further incubated with secondary antibodies (mouse, 1:10000 dilution, A9044, Sigma; rabbit ...
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bioRxiv - Neuroscience 2020Quote: ... Gels were transferred to a polyvinylidene difluoride membranes (PVDF; Merck Millipore Ltd., Tullagreen, Ireland) at 35V for 16-18 hours at 4°C ...
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Transcriptome analyses reveal tau isoform-driven changes in transposable element and gene expressionbioRxiv - Neuroscience 2021Quote: ... Membranes were probed with primary antibodies 4R tau (Millipore Sigma, cat no. 05-804), 3R tau (Millipore Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... and transferred onto Immobilon FL PVDF or P PVDF membranes (Millipore, Billerica, MA, USA) at 30V for 30-120 min ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were next incubated with secondary antibodies against mouse (1:20000, Sigma A5906) or rabbit (1:20000 ...