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Citations for Millipore Sigma :
4451 - 4500 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Cells were then labelled with primary anti-RNAP-II antibodies (CTD Antibody, clone 8WG16, Sigma-Aldrich 05-952-I) and anti-VP (a gift from Anne-Sophie Gosselin Grenet ...
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bioRxiv - Molecular Biology 2023Quote: ... The beads were separated on a magnet and resuspended in 25 µl of cold Antibody buffer (Wash buffer with 0.1% BSA) containing respective primary antibody or IgG (TFE3, Sigma HPA023881 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in the primary antibody (anti-Kv3.2 antibody, 1:200, Cat.no. APC-011, alomone lab, Israel; anti-GAD67 antibody, 1:200, Cat.no. MAB5406, Sigma, USA) at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... The membranes probed with the appropriate primary antibodies were incubated with secondary antibodies linked to horseradish peroxidase (HRP) (Sigma). Blots were revealed using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... Mouse monoclonal anti-FLAG M2 antibody (F1804) and mouse monoclonal anti-β-actin antibody (A1978) were obtained from Sigma. HRP-conjugated goat anti-rabbit (CST#7074 ...
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bioRxiv - Genomics 2024Quote: ... Samples were incubated with primary antibodies (mouse anti-acetyl-alpha tubulin antibody, clone 6-11B-1 [Millipore-Sigma Aldrich, cat# ...
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bioRxiv - Molecular Biology 2023Quote: ... an anti-GFP polyclonal antibody (Medical & Biological Laboratories) and a rabbit horseradish-peroxidase-linked IgG whole antibody (Sigma-Aldrich) were used as the primary and secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...
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bioRxiv - Developmental Biology 2023Quote: The following primary antibodies were used in this study: rabbit anti-EBF1 antibody (1:1000, AB10523; Millipore, Darmstadt, Germany,), mouse anti-MYO7A antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: Other primary antibodies used were: Atlas Antibody anti-MYO18A (Cat# HPA021121, RRID:AB_1854250) from Sigma-Aldrich (St. Louis, MO, USA); Proteintech (Rosemont ...
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bioRxiv - Molecular Biology 2023Quote: The following primary antibodies were used in this study: anti-v-Src (OP07) antibody was purchased from Sigma-Aldrich. Anti-phospho-Src family (pY416 ...
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bioRxiv - Microbiology 2023Quote: ... The primary antibodies were followed by respective species-specific secondary antibodies conjugated to HRP (Anti rabbit, 1/20000, Sigma) or (Anti mouse ...
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bioRxiv - Molecular Biology 2023Quote: ... The magnetic beads were then conjugated with anti-FLAG antibodies by adding 1 µg anti-FLAG antibody (Sigma, #F3165), followed by a 30-minute incubation at RT with rotation ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies were used at the following concentrations: Primary antibodies used were Flag-M2 - 1:1,000 (Sigma, St. Louis, Missouri) and CdnL −1:2,500 (33) ...
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bioRxiv - Pathology 2023Quote: ... Primary antibodies were incubated overnight at room temperature and diluted in antibody diluent (Sigma Aldrich, St. Louis, MO, USA) as follows ...
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bioRxiv - Cell Biology 2022Quote: ... They were then blocked in 10% goat serum and incubated with primary antibodies (anti-phosphotyrosine antibody (4G10; EMD Millipore), 1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody, A3682, Sigma Aldrich). All three blots were developed for 9.2 s after adding SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... Anti-mouse and anti-rabbit IgG horseradish peroxidase (HRP)-linked antibodies (Amersham) and streptavidin HRP-conjugated antibodies (Sigma-Aldrich) were used for ECL detection Reagents (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... or NK92 cells with antibodies against acetylated (Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse, Sigma-Aldrich, Cat# T6793) and detyrosinated (Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker ...
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bioRxiv - Cancer Biology 2024Quote: ... Beads were re-suspended in antibody buffer (50 μL) with BG4 (∼5 μM, 4 μL/sample) or H3K27ac antibody (0.5 μL/sample, Millipore) and incubated at 4 ◦C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... with two PBS washes after each incubation: primary antibody solution for 1 hour (Mouse Anti-phospho-Histone H2A.X Ser139 Antibody, clone JBW301, Sigma #05-636 ...
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bioRxiv - Molecular Biology 2024Quote: ... primary antibodies were incubated overnight at 4 °C and secondary antibodies with 0.2 mg/ml bisBenzimide Hoechst (Sigma-Aldrich) or 1 µg/ml DAPI (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... as the primary antibody in combination with a peroxidase-coupled secondary anti-mouse antibody (1:10,000 diluted; Sigma-Aldrich). The ECL reagent (Cytiva AmershamTM ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody used for all stains was an anti-5-HT antibody raised in rabbit (Sigma Aldrich, S5545), with an AlexaFluor 594 conjugated donkey anti-rabbit secondary antibody (Abcam ...
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bioRxiv - Biochemistry 2024Quote: ... Rabbit polyclonal anti-β-arrestin antibody (A1CT)18 (1:3000) and mouse monoclonal anti-α-Tubulin antibody (Sigma-Aldrich) (1:18000 ...
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bioRxiv - Biophysics 2021Quote: ... The labeled protein concentration was measured using the Direct Detect spectrometer (Merck Millipore). The degree of labeling was calculated as the ratio of the dye concentration and the protein concentration ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were transferred onto an Immobilon FL PVDF membrane (Merck Millipore, Darmstadt, Germany), probed with antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Supernatants containing chromatin fragments were pre-cleared with protein A agarose beads (Millipore) for 20 min and incubated with a specific antibody overnight at 4 °C on a nutator (antibody information were listed in Supplementary Table 1).then added 50 ul of protein A agarose beads and incubated for 2 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Completeness of protein transfer was determined by Ponceau S (Sigma-Aldrich, cat# P7170) staining for 5-6 min followed by frequent washing with H20 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were immunoprecipitated by incubating cell extracts overnight with either FLAG M2 (Sigma) or GFP (Clonetech ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were then transferred to Immobilon-P transfer membranes (PVDFs) (IPVH00010; Millipore). The membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBST ...
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bioRxiv - Cell Biology 2020Quote: ... 300µg of proteins were incubated with 30µl of Streptavidin-Agarose beads (Sigma, CS1638) on a rotating wheel at 4°C for 3hr ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins samples were resolved by SDS-PAGE and transferred to PVDF membranes (Millipore) for Western blot analysis.
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bioRxiv - Immunology 2021Quote: ... The deglycosylated protein was desalted and concentrated using Amicon filter (Merck Millipore, USA). The native protein and enzyme treated proteins were separated through SDS-PAGE and the proteins were transferred to the Nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio Rad) ...
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bioRxiv - Genetics 2021Quote: ... 1 mM phenylmethylsulfonyl (PMSF) and 1% cocktail protein protease and phosphatase inhibitors (Sigma), pH 8.0) ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatant was mixed with 30 μL of protein A Sepharose (Sigma P3391) and rotated for 2 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and filtering through 0.65 µm low protein binding filter plates (Millipore, Burlington, USA).
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bioRxiv - Microbiology 2021Quote: ... Purified protein was further concentrated using Amicon Ultra Centrifugal Filter Units (Merck Millipore).
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bioRxiv - Developmental Biology 2021Quote: Proteins were transferred to 0.45 μm Immobilon-P PVDF membrane (Millipore, Burlington, MA) in transfer buffer (25 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Priming was done with 50μg of protein in complete Freund’s adjuvant (Sigma, India) per mouse ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins associated with 3xFLAG-tagged MBD3 were purified using anti-FLAG sepharose (Sigma) and processed for mass spectrometry as described (Smits et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... 50μL of pre washed Magna ChIP protein A+G magnetic beads (Millipore Sigma) were then added to the samples and rotated at 4°C for 2 hours to elute the chromatin:antibody complexes ...
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bioRxiv - Immunology 2022Quote: ... were coated with 20μg/ml OVA protein (Sigma Aldrich, St Louis, MO, USA) or 4μg/ml CII (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with 100 μg/ml of 3×FLAG peptide (Sigma). The eluted protein was adjusted to desired concentration via Vivaspin (Sartorius ...
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bioRxiv - Biophysics 2022Quote: ... gels were stained for 1 h using ReadyBlueTM Protein Gel Stain (Sigma-Aldrich) and destained with ddH2O overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... The captured DNA-RNA hybrids were enriched by protein A-agarose beads (Millipore). The purified DNA was used to construct a library with the NEXTflex Rapid DNA-seq Kit (PerkinElmer).
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bioRxiv - Molecular Biology 2020Quote: ... the protein is transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore Corp., MA, USA) at 20 V ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluates were incubated with 40 μl of S-protein-agarose beads (Millipore) for 2 hr at 4 °C and then washed three times with NETN buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on 6% polyacrylamide gel and transferred on PVDF membrane (Millipore) using semi-dry blotting ...
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bioRxiv - Cancer Biology 2020Quote: ... FLAG-tagged fusion proteins were immunoprecipitated using Anti-FLAG M2 magnetic beads (Sigma) according to manufacturer’s instructions ...