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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for West Nile virus 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Lysates were then treated with Benzonase (Millipore, 71205-3) for 1 h at 37°C with intermittent mixing ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates were prepared using RIPA buffer (Merck/Millipore). SDS-PAGE and western blotting were performed using standard protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were mixed with 4 × Laemmli sample buffer (Sigma) and boiled for 10 min at 95°C ...
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bioRxiv - Biophysics 2023Quote: ... The lysates were then incubated on Ni-NTA (Millipore) columns for 60 min at 4 °C and washed with lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates were incubated with GSH beads (Sigma, G4510) at 4°C for 5 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell lysates were incubated with 10µl of benzonase (Sigma) per 100µl of RIPA buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... Immunoprecipitative antibody was RUNX1 (HPA004176, SIGMA, 5ug/mg lysate). Primary antibodies were RUNX1 Ms (sc-365644 ...
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bioRxiv - Cancer Biology 2022Quote: ... for western blot was from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). HRP-conjugated anti-rabbit (ref. A0545) was purchased from Sigma.
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bioRxiv - Evolutionary Biology 2024Quote: ... Chemiluminescent signals on blots were detected using SuperSignal West Femto Maximum Sensitivity Substrate (Millipore Sigma) imaged an ImageQuant LAS 500 Chemiluminescence CDD Imager (GE Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... Virus stock was filtered by centrifugation using Amicon Ultra-15 Centrifugal filter unit (Sigma, Cat # UFC910096) and resuspended in viral propagation media ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting supernatant or ‘secondary virus’ was filtered using 0.45 μm pore size sterile filter (Millipore) and was used immediately to infect large-scale expression cultures ...
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bioRxiv - Cell Biology 2022Quote: ... the medium containing the virus particles was recovered and concentrated with Polyethylene Glycol 8000 (Sigma-Aldrich) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... cells were infected with serial dilutions of virus diluted in 1× minimum essential medium (MEM, Sigma) supplemented with 0.6% BSA (Sigma) ...
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bioRxiv - Genomics 2020Quote: ... HeLa cells were incubated overnight in growing media plus virus and polybrene (Sigma-Aldrich, TR-1003). The following day ...
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bioRxiv - Molecular Biology 2019Quote: ... Supernatant was collected after 48hrs and 72hrs and virus was concentrated using Polyethylglycol (PEG, Sigma # P4338). V6.5 cells were infected in medium containing 5ug/ml polybrene (Millipore ...
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bioRxiv - Microbiology 2021Quote: Virus propagation was performed in Vero E6 cells in DMEM (Sigma-Aldrich, St Louis, MO, USA) supplemented with 2% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... FFUs were visualized using MAB8705 Anti-dengue Virus Complex Antibody clone D3-2H2-9-21 (Millipore) and goat anti-mouse Alexa Fluor 488 (A-11001 ...
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bioRxiv - Cell Biology 2019Quote: ... the virus containing suspension from HEK293T cells was filtered with a 0.45 μM filter (EMD Millipore), supplemented with polybrene (4 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... Virus was added at the indicated titer in the presence of polybrene (2µg/mL, Sigma, H9268) and the cells were incubated for 24 h ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100µl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... All virus propagation was performed in VeroE6 cells cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... The virus containing supernatant was mixed with polyethylene glycol 6000 (Sigma Aldrich, Saint Louis, Missouri, US) (8 % wt/v ...
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bioRxiv - Systems Biology 2022Quote: ... virus particles were precipitated by adding 50% (wt/vol) polyethylene glycol 6000 (PEG 6000) (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... culture supernatants containing virus were harvested and passed through a 0.45 μM PVDF filter (Millipore, SLHV033RS). Viral titers were calculated and an MOI of 1 was used for transduction ...
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bioRxiv - Cancer Biology 2024Quote: ... and concentrated using fast-trap virus purification and concentration kit according to manufacturer′s instructions (Millipore). For transduction ...
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bioRxiv - Microbiology 2024Quote: ... the virus/glycan-mixture is incubated with 0.5 U heparinase I and III (H3917, Sigma-Aldrich) for 1 h at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysates were incubated with protein G agarose (IP04, Millipore), Flag affinity agaros (A2220 ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were precleared with protein A-Sepharose beads (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were extracted in RIPA buffer (Sigma-Aldrich, R0278) and E-cadherin (MSD ...
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bioRxiv - Neuroscience 2021Quote: ... The crude lysate was treated with 250 U benzonase (Sigma) per 1 ml of lysate at 37 °C for 1.5 h to degrade genomic and unpackaged AAV DNA before centrifugation at 3000 × g for 15 min to pellet cell debris ...
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bioRxiv - Cancer Biology 2019Quote: ... Total cell lysates were extracted by radioimmunoprecipitation assay buffer (Millipore) supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... the cell lysates were incubated with M2-agarose beads (Sigma; 40 µl M2-agarose /CL6B sepharose 1:1 slurry mix ...
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bioRxiv - Biochemistry 2020Quote: ... lysates were incubated with anti-FLAG M2 beads (Sigma Aldrich) or with anti-GFP antibody (Covance ...
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bioRxiv - Cell Biology 2019Quote: ... bacterial lysates were supplemented with 0.1% Tween-20 (Sigma P7949) and incubated with equilibrated glutathione sepharose beads (FisherScientific 45000139 ...
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bioRxiv - Developmental Biology 2019Quote: ... Lysates were incubated with anti-Flag-agarose beads (A2220, Sigma) or protein A-Sepharose beads (101041 ...
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bioRxiv - Systems Biology 2019Quote: ... Separated protein lysates were transferred to PVDF membranes (Millipore #IPVH00010), which were subsequently rinsed in TBST (Tris-buffered saline ...
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bioRxiv - Biochemistry 2019Quote: ... coli lysate by Ni2+-affinity chromatography using HisSelect resin (Sigma), followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Cancer Biology 2020Quote: ... lysates were incubated with anti-FLAG M2 affinity beads (Sigma) for 2 hr at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were incubated with Anti-FLAG M2 Magnetic Beads (Sigma), washed to remove unbound proteins ...
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bioRxiv - Cell Biology 2021Quote: ... lysates were incubated with anti-myc agarose beads (Sigma, A7470) or anti-FLAG agarose beads (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were incubated with anti-Flag M2 agarose (Sigma) at 4°C at least 6 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were incubated with either IgG (anti-rabbit IgG, Sigma) or GFP-nanobody (expressed from pDZ580-pET28a-GBP and purified as described in-conjugated magnetic beads (Dynabeads M-280 ...
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bioRxiv - Plant Biology 2020Quote: ... The nuclei lysate was precipitated with anti-Flag (Sigma-Aldrich), anti-Myc (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were incubated with Anti-FLAG M2 Magnetic Beads (Sigma), washed to remove unbound proteins ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were then incubated with glutathione-agarose resin (Sigma-Aldrich) for 2 hours rotating at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: Cell lysates were prepared in RIPA buffer (Sigma Aldrich # R0278) supplemented with dithiothreitol (Life Technologies # 15508013) ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were prepared with RIPA buffer (R0278, Sigma) containing protease (cOmplete ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were precleared with protein G-sepharose 4B beads (Sigma) and then incubated with anti-HA antibody (HA.11 ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were collected by processing with RIPA buffer (Sigma), and protein concentrations were quantified using a BCA kits (Pierce Chemical) ...