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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The chopped and digested pieces were placed in a serrated 60mm petri dishes and supplemented with 10ng/μl each of human recombinant epidermal growth factor (hEGF, Cat # E9644, Sigma-Aldrich), N2 suppliment-1X (Cat # 17502048 ...
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bioRxiv - Genomics 2020Quote: ... RPMI-1640 medium used to culture the MCF7 cells was additionally supplemented with 0.01 mg/ml human recombinant insulin (Sigma Aldrich, #I3536).
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bioRxiv - Cell Biology 2021Quote: ... 250 µl of maintenance medium (50% Wnt3a-conditioned medium (ATCC#CRL-2647, Manassas, VA, USA) and 50% of 2× basal medium supplemented with recombinant human EGF (Sigma-Aldrich), recombinant human noggin (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... the native or reconstituted chromatin samples (140 μg DNA/ml) in HNE were treated with 25 μg/ml human recombinant PAD4 enzyme (Sigma Aldrich cat # SRP0329 or Cayman Chemical cat ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant Human Protein #PHG0264), 10 ng/ml epithelial growth factor (EGF, Recombinant Human Protein #PHG0311) and 10 µM ROCK Inhibitor Y-27632 (Sigma, #Y0503) were added to the media freshly before use ...
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bioRxiv - Cell Biology 2023Quote: In vitro kinase assays were performed in the presence or absence of different recombinant human atypical PKC (50 or 100 ng of PKC ζ, #14-525 Merk Millipore; 1µg of PKC I/αPar6 complex ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µM carnitine) with or without supplementation of 100 ng/ml (13.16 nmol/l) IGF1 (human recombinant IGF1, I3769, Sigma-Aldrich, Germany). Medium was changed three times per week and 48 hours before harvest ...
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bioRxiv - Cell Biology 2024Quote: ... was added to the cell pellet and stored at −20°C prior to being assayed for insulin using human insulin specific RIA (Millipore Cat# HI-14K).
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged and non-Fc-tagged proteins were concentrated using Amicon Ultra 10 kDa MWCO centrifugal filter units (Millipore), dialyzed against PBS ...
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bioRxiv - Biophysics 2021Quote: ... or anti-His antibodies (Sigma- Aldrich).
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bioRxiv - Microbiology 2019Quote: ... or anti-His (1:1000 Sigma) overnight at 4 °C and anti-mouse secondary (1:5000) ...
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bioRxiv - Microbiology 2021Quote: ... α-His (Sigma, cat# H1029, 1: 3,000), α-ICDH (1 ...
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bioRxiv - Microbiology 2020Quote: ... α-His(Sigma, cat# H1029, 1: 10,000), α-HA (Santa Cruz ...
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bioRxiv - Biochemistry 2019Quote: His-select Cobalt affinity beads (Sigma) were equilibrated in binding buffer (40 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-His (Sigma-Aldrich, H1029, RRID:AB_260015), anti-Strep (IBA GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His antibody (EMD Millipore) as described previously [39] ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His antibody (EMD Millipore) as described previously [39] ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-HIS (Sigma H1029 1:2,500) or anti-MBP (New England Biolabs E8032 ...
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bioRxiv - Microbiology 2021Quote: ... recombinant hyaluronidase (Sigma, H3506) was treated at 10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... recombinant neuraminidase (Sigma, N2876) was treated at 1 U/mL ...
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bioRxiv - Immunology 2022Quote: ... recombinant CD4 (Sigma Aldrich) were added to 20 μl of KSRB with 1mM APMA ...
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bioRxiv - Immunology 2021Quote: Small interfering RNA (siRNA) to human 3’ UTR PKC-θ was purchased from Sigma Aldrich. YTS-2DL1 cells were transfected with siRNA specific for the indicated genes ...
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bioRxiv - Immunology 2019Quote: ... Coverslips were washed with PBS and coated with 3% human serum albumin (HSA; A1653, Sigma) o.n ...
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bioRxiv - Bioengineering 2024Quote: ... A 3 μL drop of 2.5 U/mL human thrombin (Sigma-Aldrich, cat. no. T6884) was added to each well of the platform ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Microbiology 2023Quote: ... PhoQ-His and Flag-MgrB were detected with the anti-His and anti-Flag primary antibody (Sigma), respectively ...
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bioRxiv - Cancer Biology 2019Quote: HeLa was transfected with Flag-tagged Vav1 and V5-tagged VEGFR1 grown on coverslips were stained with antibody against Flag-tag (Sigma), and against V5-tag (Cell Signaling) ...
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bioRxiv - Developmental Biology 2020Quote: ... HA-tagged or Myc-tagged proteins were isolated with anti-HA agarose or anti-c-Myc Agarose Affinity Gel (Sigma), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... PTP-tagged or MHTAP-tagged protein variants were detected using the peroxidase-anti-peroxidase soluble complex (PAP) (1:2000, Sigma) as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... individual components can be added to 500 ml media bottle at the indicated final concentrations: 1) Recombinant Human Insulin (Sigma I-1882) – 12.5 µg/ml final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were then allowed to grow for 5 days followed by 24 hour treatment with recombinant human protein TNF-α (H8916, Sigma Aldrich) at 40ng/ml or IFN-gamma (PHC4031 ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 ng/ml human recombinant epithelial growth factor and 50 U/ml penicillin and 50 U/ml streptomycin (Sigma-Aldrich, MO, USA). The VK2 cells were seeded into 24-well tissue culture plates ...
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bioRxiv - Developmental Biology 2022Quote: ... containing the same supplements) freshly supplemented with 25 ng/mL of human recombinant FGF4 (prepared in house) and 1 μg/mL heparin (Sigma-Aldrich, USA). TSCs were passaged every 3-5 days ...
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bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
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bioRxiv - Immunology 2024Quote: ... The coating of the Ibidi® µ-slides IV 0,4 Uncoated (Cat# 80601) with fibrin was performed by adding PBS mixed with recombinant human albumin (Sigma-Aldrich, Cat# A9731), fibrinogen from human plasma AF488 Conjugate (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... GST tagged was bought from Sigma-Aldrich (SRP5172). G-actin and biotinylated actin were bought from Cytoskeleton ...
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bioRxiv - Neuroscience 2021Quote: ... the proteins were tagged with FITC (Sigma, F7250) before encapsulation.
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bioRxiv - Cancer Biology 2022Quote: ... HRP-tagged anti-goat secondary antibody (Sigma-Aldrich), GFP ...
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bioRxiv - Neuroscience 2023Quote: ... and anti-mouse FITC tagged (1:200, Sigma) at room temperature in dark for 90 min ...
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bioRxiv - Biochemistry 2021Quote: ... they were eluted through a 22-cm-long Sephadex-G75 (Sigma-Aldrich) column pre-equilibrated with the assay buffer to remove the external calcein ...
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bioRxiv - Microbiology 2019Quote: ... Removal of His-tag was verified by western blot with monoclonal α-His –HRP (1:10000) antibody (Sigma).
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bioRxiv - Microbiology 2020Quote: ... HTF-tagged LsaA and FLAG3-tagged VgaALC proteins were detected using anti-Flag M2 primary (Sigma-Aldrich, F1804; 1:10,000 dilution) antibodies combined with anti-mouse-HRP secondary (Rockland ...
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bioRxiv - Cell Biology 2023Quote: ... human Spindly siRNA (Sequence antisense: 5’-GAAAGGGUCUCAAACUGAA-3’, dTdT overhangs, Sigma-Aldrich, St. Louis, MO, USA), and control siRNA (D-001810-10-05 ...
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bioRxiv - Plant Biology 2020Quote: ... coli using a Nickel-His column (Sigma). A 5 mL culture was incubated at 37°C for 16 hours ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His tag (HIS.H8, 1:1,000, Millipore). The HRP-conjugated anti-mouse (111–035-144 ...
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bioRxiv - Plant Biology 2020Quote: ... -His with or without 3AT (Sigma-Aldrich). Successful interactions were further screened on –Leu ...
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bioRxiv - Microbiology 2020Quote: ... The anti-his antibody (monoclonal #8722 Sigma) was used at 1/2000 concentration for immunoblot.
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bioRxiv - Biochemistry 2022Quote: ... and anti-His (1:3000) antibody (Sigma). HRP conjugated mouse IgG was used at 1:5000 dilution (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-His HRP conjugated (Sigma-Aldrich) antibodies ...