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Citations for Millipore Sigma :
401 - 450 of 3335 citations for ETS1 EWSR2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Human platelets were stimulated with 1 U ml−1 Thrombin from human plasma (Sigma-Aldrich) or left unstimulated for 30 min to assess the purity and activation of isolated cells ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used procollagen VII (rabbit anti–human [Abcam]; mouse anti-human [Sigma-Aldrich]), Sec31A (mouse anti–human ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation, Billerica, MA, USA), Fluo-4 AM and Pluronic F-127 (Molecular Probes ...
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bioRxiv - Pathology 2024Quote: ... RNA from human liver tissue and human cirrhotic PCLS was isolated using Tri Reagent (T9424, Sigma) and 1-bromo-3-chloropropane (B9673 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5□µg/ml human insulin (Sigma), 100 U/ml penicillin and 100□μg/ml streptomycin (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... human epidermal growth factor (hEGF; Sigma), recombinant human basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2021Quote: ... Commercially available human serum (Sigma-Aldrich) was used as the positive control.
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bioRxiv - Biochemistry 2020Quote: ... human liver cathepsin B (Millipore Sigma), and human cathepsin K (Enzo Sciences ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-human nuclei (Millipore MAB1281), mouse anti-TUJ1 (Covance MMS 435P) ...
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bioRxiv - Developmental Biology 2020Quote: ... of human chorionic gonadotrophin (hCG) (Sigma) 46 hours later ...
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bioRxiv - Developmental Biology 2020Quote: ... human insulin was purchased by SIGMA and was used at a final concentration of 7ug/ml ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with human albumin (Sigma, #A0237), sodium DL-lactate (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... human serum albumin (Sigma, Catalog # A9511) and human pituitary FSH (National Hormone and Pituitary Program ...
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bioRxiv - Immunology 2021Quote: ... purified human C1q (10μg/ml; Millipore) was added as a source of complement for 30 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 0.5% human serum albumin (Sigma Aldrich). Cell number in the suspension was adjusted to achieve a λ (mean number of cells per droplet ...
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bioRxiv - Cell Biology 2020Quote: ... TANGO1 (rabbit anti–human; Sigma-Aldrich), sec16A (rabbit anti-human ...
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bioRxiv - Bioengineering 2019Quote: ... human fibronectin (Merck-Millipore, Watford, UK) was premixed with Laponite gels to a final concentration of 50 µg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... sec16A (rabbit anti-human; Sigma-Aldrich), calreticulin (goat anti–human ...
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bioRxiv - Immunology 2019Quote: ... plus 10% human serum (Sigma,UK). IL-2 (100 IU/ml ...
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bioRxiv - Biochemistry 2020Quote: ... (iv) 10 nM human insulin (Sigma), and (v ...
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bioRxiv - Microbiology 2019Quote: ... Binding of human FH (Merck Millipore) was detected with Alexa-Fluor-488 labeled human FH ...
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bioRxiv - Biochemistry 2020Quote: ... 40 μM human serum albumin (Sigma) was included in the reaction ...
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bioRxiv - Biophysics 2021Quote: ... 10 µg/ml human insulin (Sigma) and penicillin (100 U/ml ...
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bioRxiv - Neuroscience 2020Quote: ... albumin (human serum, Millipore Sigma #A9511), hemoglobin (human ...
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bioRxiv - Systems Biology 2020Quote: Human holo-transferrin (Sigma, cat: T4132) was coupled with thiorhodamine SOG at a molar ligand:SOG ratio of 1:1 and purified using 7-kDa ZebaSpin Columns (Thermo ...
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bioRxiv - Immunology 2021Quote: ... and human (AB serum, Sigma-Aldrich) in NuncTM 96 Deep-Well polystyrene plates (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... To aggregate human IgG (I4506; Sigma), its solution (2 mg/mL in PBS ...
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bioRxiv - Microbiology 2020Quote: ... Lyophilized human complement serum (Sigma, USA) was reconstituted in sterile phosphate-buffered saline (PBS) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg/mL human transferrin (Sigma) and 30 nM sodium selenite (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... Human ARID1A antibody (Millipore: (04-080) Anti-BAF250a/ARID1a Antibody ...
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bioRxiv - Neuroscience 2022Quote: ... and 1μM recombinant human insulin (Sigma). Organoids were collected from the wells on day 14 and transferred to 10cm dishes at roughly 20 organoids per dish ...
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bioRxiv - Physiology 2022Quote: ... 500μg/ml human recombinant albumin (Sigma), 213μg/ml L-ascorbic acid (Sigma) ...