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Citations for Millipore Sigma :
4401 - 4450 of 10000+ citations for Recombinant Human SDC2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and the protein concentration was determined with Bradford reagent (Sigma-Aldrich). Lysates were subsequently denatured in Laemmli sample buffer for 5 minutes at 95°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins bound to anti-Flag M2 affinity beads (Sigma-Aldrich) were recovered from the supernatants and washed by Flag wash buffer (50 mM NaPi (pH 7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were subsequently transferred to a 0.45 µm PVDF membrane (Millipore). Primary antibodies were used at the following dilutions in 5% Blotting-Grade Blocker (BioRad) ...
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bioRxiv - Physiology 2023Quote: ... proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, MA) overnight ...
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bioRxiv - Genetics 2023Quote: ... PREPL proteins were immunoprecipitated using anti-FLAG M2 antibody (Sigma Aldrich) and separated by SDS-PAGE ...
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bioRxiv - Genetics 2024Quote: ... the proteins were transferred onto a nylon membrane (Immobilon-P, Millipore) and incubated with primary antibodies in a blocking buffer (1ξ PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... protein lysates were prepared in 1× RIPA buffer (Millipore, #20-188) from Drosophila head tissue expressing UAS-FLAG-Dx ...
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bioRxiv - Immunology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2-agarose (Sigma), following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... and pellets were resuspended in BugBuster® protein extraction reagent (Millipore). Protein from the lysed cell suspension was then extracted by chloroform/methanol precipitation ...
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bioRxiv - Genomics 2024Quote: ... The protein concentration was measured with the Bradford Assay (Sigma-Aldrich). Aliquots of the extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Amersham/GE Healthcare) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined by a Bradford assay (Sigma Aldrich; B6916). Proteins were denatured in NuPAGE™ LDS Sample Buffer (4X ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto Immobilon-P PVDF membranes (Millipore, Darmstadt, Germany) in 1× Tris Glycine with 20% ethanol ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated using Amicon Ultra centrifugal filter units (Merck Millipore). Protein was then aliquoted ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins in the gel bands were reduced with dithiothreitol (DTT) (Sigma) and alkylated by iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was concentrated using Amicon Ultra Centrifugal Filter units (Millipore) and further purified by size exclusion chromatography using a Superdex 75 prepgrade column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was blotted off with filter paper (Sigma–Aldrich, WHA1001110), and immediately after blotting ...
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated with Amicon Ultra-15 centrifugal filters (Millipore) and buffer exchanged to storage buffer (20 mM Tris-HCl ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... After transferring the proteins to PVDF membranes (Millipore, Etobicoke, ON, Canada) as described ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins then transferred to 0.45 µM Immobilon-FL PVDF (Millipore Sigma) after methanol activation ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were transferred onto PVDF membranes (Millipore, Billerica, MA, USA), the membranes were quenched and proteins were detected using specific antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... protein was extracted by using RIPA lysis buffer (20-188, Millipore) supplemented with cOmplete Mini protease and phosphatase inhibitors tablets (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein samples were then concentrated using Amicon 3-kDa concentrators (Millipore), and the purity of each protein was assessed by SDS-PAGE and further confirmed by Western blotting using commercial antibodies against the strep tag (Biobharati Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... the protein was transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore), and proteins were detected by probing with specific antibodies (Table S7 ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated with PureProteome protein A/G magnetic beads (Sigma) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... along with several dilutions of a SOD1 protein standard (Sigma, S9697) were separated on native 12% Tris-Glycine acrylamide gels (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to an Immobilon polyvinylidene difluoride (PVDF) membrane (Millipore) using a wet transfer Mini Trans-Blot Cell (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... filtered through a 0.45-µm low-protein-binding Durapore filter (Millipore), and stored at −80°C until use ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were concentrated with a 30 kDa cutoff Amicon concentrator (Millipore), and gel-filtered on a Superose 6 10/30 (GE Healthcare ...
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bioRxiv - Developmental Biology 2023Quote: ... strep tag was removed from the eluted proteins by thrombin (Sigma) digestion overnight at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... total protein amounts were assayed using Fast Green FCF (Sigma, #F7252) staining solution47 and imaged using LICOR Odyssey 700 nm ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins in gel was visualized with sliver staining kit from Sigma. The gels over 100 KDa (SATB2 molecular weight ...
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bioRxiv - Microbiology 2023Quote: ... lysates were incubated with PureProteome protein A/G magnetic beads (Sigma) for 1-2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was quantified using a BCA assay or by Millipore Direct Detect ...
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bioRxiv - Neuroscience 2023Quote: ... The protein coated membrane was blocked with 5% normal serum (Sigma) and 5% skim milk (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... and monoclonal anti-glial fibrillary acidic protein (GFAP) IgG (G3893, Sigma) and secondary antibodies Alexa Fluor 647 and Alexa Fluor 488 were applied to label neurons and astrocytes ...
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bioRxiv - Neuroscience 2023Quote: ... and amyloid precursor protein (APP) (A8717, Sigma-Aldrich, St. Louis, MO) or monoclonal antibody against β-actin (A2228 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to polyvinylidene difluoride membranes (PVDF; Merck Millipore Ltd.) at 35 V overnight in a cold room at 4°C ...
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bioRxiv - Genetics 2023Quote: ... Proteins were transferred to Immobilon-P membranes (Millipore; cat. no. IPVH85R) at 120 V for 50 min on ice in 1X tris-glycine-methanol transfer buffer (25 mM tris base ...
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bioRxiv - Physiology 2023Quote: ... Protein concentrations were determined using a bicinchoninic acid assay (Sigma, #BCA1), and the resulting absorbance was read at 562 nm ...
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bioRxiv - Biochemistry 2023Quote: ... protein tags were removed by tobaccoetchvirus (TEV) protease (#T4455, Sigma-Aldrich) (1:25 w/w ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to the polyvinylidene fluoride (PVDF) membrane (Millipore, USA) which was subsequently fixed with 8% acetic acid for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were visualized using Immobilon Forte Western HRP Substrate (Millipore, WBLUF0500).
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bioRxiv - Molecular Biology 2023Quote: Total proteins were extracted with RIPA buffer (Sigma Aldrich, Missouri, USA) supplemented with protease and phosphatase inhibitor cocktail then dosage was carried out with BCA protein quantification kit (Life Technologies ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Pure protein solutions were concentrated by centrifugation in Centricon tubes (Millipore).
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bioRxiv - Developmental Biology 2023Quote: ... and then the proteins were transferred to the PVDF membranes (Millipore). After being blocked by the 3% BSA (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were transferred to Immobilon-P transfer membranes (Sigma-Aldrich) using the Trans-Blot® TurboTM Transfer System (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were extracted as whole cell lysate using trichloroacetic acid (Sigma) and glass beads as described before87.
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bioRxiv - Developmental Biology 2022Quote: Proteins were transferred to 0.45 μm Immobilon-P PVDF membrane (Millipore) in transfer buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... For conditional induction of protein expression 1μg/ml of doxycycline (Sigma) was added to the culture media and incubated for 16-24h.
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bioRxiv - Cell Biology 2022Quote: ... Transfer of protein on activated PVDF membranes (EMD-Millipore, cat#IPVH07850) were performed overnight at 4°C at 50mA ...