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Citations for Millipore Sigma :
4401 - 4450 of 10000+ citations for Recombinant Human IL34 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Protein bands were transferred to polyvinylidene difluoride (PVDF) membrane (Millipore) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations were measured by Bradford test kit (Sigma, B6916) and 10 μg/sample was loaded on a 15% polyacrylamide SDS-PAGE gel (30:1 acrylamide/bis-acrylamide) ...
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bioRxiv - Microbiology 2021Quote: ... filtered by a 0.45 µM low protein binding filter (Millipore) and medium was exchanged three times for Opti-MEM I (1X ...
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bioRxiv - Developmental Biology 2021Quote: ... and protein was transferred to a Immobilon-FL membrane (Millipore), which was incubated with goat anti-h/m GLI3 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were transferred to Immobilon PVDF 0.45 μm membranes (Millipore). Nonspecific binding was blocked by 5% Bovine Serum Albumin in Tris-buffered saline ...
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bioRxiv - Molecular Biology 2020Quote: Antibody to the GAPDH protein was purchased from Millipore (MAB374). Antibodies to the p15 (sc-612 ...
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bioRxiv - Plant Biology 2021Quote: ... and 50 μl Protein A Agarose/Salmon Sperm DNA (Millipore) at 4°C for 2 h ...
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bioRxiv - Biophysics 2020Quote: ... proteins were electrophoretically transferred onto an Immobilon-P membrane (Millipore). After transfer ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore) using a Bio-Rad transfer system at 90 V for 80 min ...
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bioRxiv - Plant Biology 2021Quote: ... Purified proteins were concentrated by ultrafiltration (Amicon Ultra system, Millipore) and stored at −80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... the proteins were transferred to polyvinylidene difluoride (PVDF) membrane (Millipore). Membranes were blocked in 5 % low fat milk in PBS and then incubated with primary antibodies in 0.1 % Tween- PBS at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were treated with benzonase (200 U/mg protein, Sigma) for 5 min and then clarified by centrifugation at 15,000 g for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... and filtered through a 0.45um low protein binding filter (Millipore) to remove debris ...
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bioRxiv - Neuroscience 2021Quote: ... Separated proteins were transferred onto PVDF Immobilon P membrane (Millipore) using a Mini Trans-Blot (Biorad ...
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bioRxiv - Immunology 2020Quote: ... followed by 10 nm gold-conjugated protein A (Sigma-Aldrich). Labeled cells were adhered to poly-L-lysine-coated mica strips and fixed with 0.1% glutaraldehyde ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... β-actin was used as protein loading control (Sigma A2066,1:10000). Secondary antibodies anti-rabbit ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred onto Immobilon-FL PVDF membranes (Sigma Aldrich), blocked Odyssey Blocking Buffer in TBS for 1 hour and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 % Protein G-agarose beads (30 μl) (Millipore, 16-266) were added to lysates after 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred to an Immobilin-P membrane (Millipore®) at 10 Volts for 1 h in a semi-dry apparatus (Trans-Blot® SD ...
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bioRxiv - Biochemistry 2020Quote: ... Immunoprecipitations using antibodies coupled to protein G-Sepharose beads (Sigma) were as described previously (Levy et al. ...
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bioRxiv - Bioengineering 2020Quote: ... Separated proteins were electro-transferred to PVDF membranes (Millipore, UK) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... the protein samples were transferred onto a PVDF membrane (Millipore). The transferred PVDF membrane was blocked with 3% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein spots were stained using Direct Blue (Sigma-Aldrich, Australia), excised and transferred to a flat bottom polypropylene 96-well plate (Corning Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were then transferred to a PVDF membrane (Millipore), blocked with 5% non-fat milk ...
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bioRxiv - Plant Biology 2022Quote: ... Six ml of protein extraction buffer (1 tablet of Sigma Complete™ mini protease inhibitor ...
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bioRxiv - Neuroscience 2022Quote: ... proteins were transferred to Immobilon-P PVDF membranes (Millipore, USA), followed by immunoblotting ...
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bioRxiv - Immunology 2022Quote: ... Proteins were reduced with 5 mM dithiothreitol (DTT) (Sigma-Aldrich) for 30 min at 55 °C and then alkylated by the addition of 10 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... the bound proteins were eluted with 3X FLAG peptide (Sigma). The purity of DNA2 proteins was analyzed by 4–15% gradient SDS–polyacrylamide electrophoresis (SDS–PAGE ...
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bioRxiv - Molecular Biology 2022Quote: Protein lysates were generated by incubation with RIPA Buffer (Sigma) supplemented with 1% complete protease inhibitor cocktail (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... FN3 proteins were expressed in Rosetta DE3 cells (EMD Millipore), lysed by sonication ...
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bioRxiv - Cancer Biology 2022Quote: Protein was extracted using Radioimmunoprecipitation Assay (RIPA) buffer (Sigma- Aldrich) supplemented with 1 mM EDTA ...
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bioRxiv - Neuroscience 2022Quote: ... astrocytic marker glial fibrillary acidic protein (GFAP) (chicken, Millipore, AB5541), microglial marker ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Plant Biology 2019Quote: ... before capture of immune complexes with Protein A agarose (Millipore). Crosslink reversal and protein removal was performed as described previously (Nelson et al. ...
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bioRxiv - Plant Biology 2019Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... and purified by protein A-agarose beads (16-157; Millipore). NIPBL ChIP-seq were from recently published data [46] ...
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bioRxiv - Genetics 2019Quote: ... Proteins were transferred to Immobilon-P PVDF membrane (Millipore Sigma) at 400 mA for 40 min on a Trans-blot SD semi-dry transfer cell (Bio-Rad ...
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bioRxiv - Neuroscience 2019Quote: ... 100 μM SAMS (substrate for AMP-activated Protein Kinase, Millipore), 0.2 μCi (γ-32P ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore), blocked using 5% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was eluted with 10 mM reduced GSH (Sigma) in 10 mM Tris-HCl ...
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bioRxiv - Genomics 2019Quote: ... Proteins were transferred onto Immobilion-P PVDF membranes (Millipore Sigma) by means of tank blotting in 1X Tris/Glycine (Bio-Rad ...
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bioRxiv - Physiology 2019Quote: ... G-Sepharose beads (50µL for 2mg of total proteins, Sigma) were added for 1h before being washed with lysis buffer 3 times ...
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bioRxiv - Bioengineering 2019Quote: ... 20μg protein was incubated in 10% 2-Mercaptoethanol (Sigma-Aldrich) in laemmli loading buffer (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were electroblotted onto Immobilon-P transfer membranes (Millipore Corporation). The membranes were blocked with Blocking One (Nacalai Tesque ...
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bioRxiv - Immunology 2019Quote: ... Protein concentrations were read by a MAGPIX from EMD Millipore using xPONENT 4.2.
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bioRxiv - Biochemistry 2020Quote: ... 100 µg protein were dot blotted on PVDF membrane (Millipore), stained with Alcian blue and excised ...
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bioRxiv - Zoology 2020Quote: ... in comparison with wells containing visible protein ladder (Sigma 26616). The antibody staining intensity of each Western blot sample was normalised to its corresponding total-protein stain intensity using BioRad ImageLab software ...
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bioRxiv - Biochemistry 2019Quote: ... Solubilized proteins were applied to FLAG-M2 agarose (Sigma-Aldrich) pre-equilibrated with buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... Protein expression was induced with 200 pg/mL doxycyclin (Sigma). Cell cycle synchronization was performed using a combination of 2.5 mM thymidine (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Following electrophoretic transfer of proteins onto PVDF membranes (Millipore, IPVH00010), nonspecific binding was blocked by incubation with 5% skim milk ...
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bioRxiv - Immunology 2020Quote: ... and immunoprecipitated on Sephadex protein A/G beads (Sigma Aldrich, St ...