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Citations for Millipore Sigma :
4301 - 4350 of 7667 citations for Recombinant Ovine IL4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The purified protein was desalted and concentrated with the Centricon cutoff 10 kDa (Merck Millipore). The purity of the protein was analyzed by SDS-page and Western-blot using Anti-His antibodies.
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bioRxiv - Microbiology 2020Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously (49).
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bioRxiv - Microbiology 2020Quote: ... The eluate containing protease and cleaved protein was concentrated using Amicon Ultra 10,000 MWCO (Millipore), then passed through a Superdex-200 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... MS2 capsid proteins were detected with a polyclonal rabbit antibody (ABE76-I, Merck Millipore, France).
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bioRxiv - Microbiology 2021Quote: ... coli membrane fractions were determined using a bicinchoninic acid (65) protein assay kit (Sigma-Aldrich) with BSA (Interchim ...
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bioRxiv - Microbiology 2021Quote: ... Total protein concentrations were determined using the bicinchoninic acid assay (Sigma Inc.; procedure TPRO-562), and SDS-PAGE (12% ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue lysates were precleared for 2 h with Protein A + G magnetic beads (EMD Millipore). Precleared lysates were then incubated with 5 μg of anti-Sox9 antibody (Abcam ...
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bioRxiv - Cell Biology 2020Quote: The following proteins were detected by western blotting (antibodies in brackets) CPSF3 (Sigma-Aldrich, HPA04657), IntS11 (Sigma-Aldrich ...
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bioRxiv - Pathology 2021Quote: ... Proteins were subsequently electrotransferred to a polyvinylidene fluoride (PVDF) membrane (Immobilon-P Membrane, PVDF, Millipore) for 20 min at 25 mA and 2,5 mV in a Trans-Blot Turbo TM Transfer System (BioRad) ...
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bioRxiv - Neuroscience 2019Quote: Protein lysates were collected from regional human astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins resolved by gel electrophoresis were transferred onto an Immobilon-FL PVDF membrane (EMD Millipore) at 100 Volts for 90 minutes in ice-cold transfer buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred from gels to 42-μm-pore Millipore PVDF membranes (Sigma-Aldrich, MO) for 1 h at 30 V ...
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bioRxiv - Immunology 2020Quote: ... Eighty micrograms of protein was separated by SDS-PAGE and transferred to PVDF membranes (Millipore). The membranes were blocked with milk or BSA for 1 h before incubation with primary antibodies for 1.5 h ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then concentrated to 1 mg/ml using Amicon device 100K (Merck Millipore) and stored at -80°C ...
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bioRxiv - Immunology 2020Quote: ... and 10 mg of protein per experiment were reduced with 10mM dithiothreitol (DTT, Sigma, #D0632) for 1 hour at 55°C and alkylated with 20mM iodoacetamide (IAA ...
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bioRxiv - Genetics 2019Quote: Proteins were precipitated in 23% TCA (Sigma-Aldrich, St. Louis, MO, Product number T-0699) at 4 °C O/N ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were digested with endoproteinase Lys-C (Wako Chemicals) and sequencing-grade modified trypsin (Sigma). To purify the peptides ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and the purified proteins were concentrated with Amicon Ultra-0.5 Centrifugal Filter devices (Merck Millipore) following manufactures instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The eluted proteins were concentrated 5X with 10KDa-cutoff Amicon centrifugal filter unit (Millipore, #UFC501024), and analyzed by native gels and Western blotting.
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bioRxiv - Physiology 2019Quote: ... Data were normalized by protein content of the homogenate measured by Bradford assay (Sigma Aldrich).
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bioRxiv - Cell Biology 2019Quote: ... followed by an overnight incubation with Pure Proteome Protein A/G mix magnetic beads (Millipore). Samples were washed as indicated by the manufacturer ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was concentrated using a 30 kDa MWCO centrifugal filters (Amicon Ultra; Merck Millipore) and then gel filtered ...
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bioRxiv - Cell Biology 2019Quote: ... Protein expression was induced by 0.2 mM Isopropyl-²-D-thiogalactopyranoside (IPTG; Sigma-Aldrich, Taufkirchen, Germany) and continued at 16 - 20°C for 18 - 20 h ...
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bioRxiv - Biochemistry 2020Quote: ... Protein glycosylation status was analysed by Western Blot using an anti-c-myc antibody (Sigma), anti-rabbit IgG-peroxidase conjugated (Sigma) ...
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bioRxiv - Immunology 2021Quote: S protein at 1mg/ml in PBS was cross-linked with 4% formaldehyde (FA) (Sigma) overnight at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... The remainder of the samples was incubated for 1hr with uncoated protein G beads (Millipore) to remove a-specifically bound proteins ...
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bioRxiv - Cell Biology 2021Quote: ... The protein sample was concentrated in an Amicon Ultra 15 centrifugal filter (Millipore Sigma, UFC900308) to a concentration 1mg/ml and injected into Guinea Pigs (Pocono Rabbit Farms and Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used for protein immunoblots: Anti p-Tyr (4G10, Millipore Sigma, #05-321 and P-Tyr-1000 ...
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bioRxiv - Physiology 2020Quote: ... light chain 3B protein (LC3I/II) tubulin (1:1,000; Cat. No. T-5168; Sigma-Aldrich), or mitochondrial porin (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... The protein was eluted with buffer C (buffer B+10%glycerol+2.5 mM Desthiobiotin (Novagen)) in four column volumes ...
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bioRxiv - Biochemistry 2021Quote: ... Expression of Clover-MgtA fusion proteins was induced by addition of 0.2 % L-arabinose (Sigma) in exponential growth phase for 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... Protein glycosylation status was analysed by Western Blot using an anti-c-myc antibody (Sigma), anti-rabbit IgG-peroxidase conjugated (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... Purified protein was concentrated with Amicon Ultra-0.5 mL centrifugal filters (50kDa cutoff; EMD Millipore) to 100 µL ...
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bioRxiv - Cell Biology 2021Quote: ... 8 mg protein were incubated with ANTI-FLAG® M2 Affinity Gel (Sigma, cat.no. A2220) for 2 h on a rotator at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: Total protein was extracted from thirty zebrafish embryos using cell lysis buffer (Sigma-Aldrich, C3228). Purified protein was then digested into peptides using Trypsin (Promega ...
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bioRxiv - Biophysics 2021Quote: Proteins and peptides were labelled at their N-terminus with Fluorescein 5-Isothiocyanate (FITC, Sigma), purified and quantified following a described protocol [45] ...
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bioRxiv - Neuroscience 2021Quote: ... Protein extracts were prepared and subjected to immunoprecipitation with anti-FLAG M2 magnetic beads (Sigma) as previously described [25] ...
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bioRxiv - Physiology 2021Quote: ... or amyloid precursor protein (0.6 mg/kg, sAPPα, S9564 Sigma Aldrich Solutions, St. Louis, MO) injection ...
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bioRxiv - Cell Biology 2021Quote: ... samples were treated with the protein synthesis inhibitor 10 μM cycloheximide (Millipore Sigma C4859-1ML) for 10 minutes prior to puromycin treatment and during puromycin treatment ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were digested overnight with trypsin from bovine pancreas (Sigma-Aldrich, St Louis, MO, USA) at 37 °C at a 50:1 (m/m ...
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bioRxiv - Cancer Biology 2020Quote: ... wild type and mutant proteins were treated similarly with calpain 1 (Sigma-Aldrich, Cat. #C6108) in vitro and their cleavage was assessed by immunoblotting.
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bioRxiv - Cancer Biology 2020Quote: ... The FLAG M2 antibody used for the detection of MetRSL274G protein was purchase from Sigma. ANL ...
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bioRxiv - Cell Biology 2020Quote: ... The bound protein was eluted four times with 250 μL of HA peptide (Sigma-Aldrich) (0,1 mg/ml).
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bioRxiv - Biophysics 2019Quote: ... GFP-PRC1 isoform 2 proteins were expressed via BL21(DE3) Rosetta Escherichia coli cells (Novagen). Full length PRC1 was expressed for 4 hours at 18 °C after induction with 0.5mM IPTG ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). After cell lysis ...
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bioRxiv - Microbiology 2020Quote: ... Outer membrane proteins of these bacteria were subsequently labelled with fluorescein isothiocyanate (FITC, Sigma-Aldrich) as previously described [57] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Proteins were separated by 10% SDS-PAGE gel followed by electrotransfer to PVDF membranes (Millipore). The membranes were blocked with TBST supplemented with 10% skim milk for 30 mins at room temperature and further probed with primary antibodies at 4°C overnight including Trim17 (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected using primary antibodies α-FLAG (monoclonal α-FLAG M2, Sigma, 1:1000) or α-Myc (Clone A-14 ...
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bioRxiv - Microbiology 2021Quote: ... The protein concentration was measured by Coomassie Brilliant Blue assay using bovine serum albumin (Sigma) as standard ...
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bioRxiv - Neuroscience 2021Quote: ... the isolated cell populations were immediately placed in 20μl RIPA protein extraction buffer (EMD Millipore) with HALT Protease/Phosphatase inhibitors (ThermoFisher Scientific ...