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Citations for Millipore Sigma :
4301 - 4350 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and mouse anti-puromycin (MABE343, 1:500, Millipore). Detection of newly synthesised MBP and MOBP through PLA was performed according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: CGR8 mouse ES cells (Sigma, Cat#07032901-1VL) and ZHBTc4 mouse ES cells [34] were routinely cultured at 37 °C and 5% CO2 on cell culture-treated dishes coated with 0.1% gelatin (Sigma #G9391-100G ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... and precleared using protein A agarose beads (25%, diluted in ChIP dilution buffer; Protein A Agarose/Salmon Sperm DNA, Millipore, 16-157) and incubated overnight at 4°C (final buffer composition during antibody incubation was 0.1% SDS ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined using a Bradford protein assay kit (Thermo-Fisher, MA, USA) with bovine serum albumin (Sigma-Aldrich, MO, USA) as the standard ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was determined using BCA™ protein assay (Pierce, Rockford, IL, USA) using BSA (Sigma- Aldrich, St. Louis, MO, USA) as a standard ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The soluble and insoluble protein fractions were extracted using a CelLytic™ MEM Protein Extraction Kit (Sigma–Aldrich, St. Louis, MO, USA) and a CelLytic™ NuCLEAR™ Extraction Kit (Sigma-Aldrich) ...
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bioRxiv - Genetics 2023Quote: ... and samples were analyzed by standard immunoblotting procedures using 1:3000 peroxydase-conjugated antiperoxydase (PAP, to detect protein-A- tagged proteins) (Sigma, #P1291, RRID:AB_1079562), 1:3000 monoclonal anti-FLAG antibody (Sigma ...
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bioRxiv - Biochemistry 2022Quote: The expression vector pGEX-6P-1-GST-FLAG containing the relevant fusion protein and fluorescence protein was transformed into Rosetta™ 2 (pLysS) host strains (Novagen; 71403). A single colony was inoculated in 5.5 mL LB broth (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were performed on both total proteins and proteins eluted from the anti-GFP affinity beads using anti-FLAG (Sigma-Aldrich, F31651), anti-GFP (Abcam ...
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bioRxiv - Microbiology 2024Quote: Approximately equal amounts of GST fusion proteins and alanine-mutated GST fusion proteins were separated by 12% SDS-PAGE and transferred to PVDF transfer membranes (Millipore, MA, USA). The membranes were blocked with 5% skimmed milk powder for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... the expression levels of recombinant B56 subunit and PME-1 were monitored by western blot using antibodies that specifically recognize HA-tag (Sigma, 2CA5, Cat#: 116666060011, 1:1000) and PME-1(Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... Prior to labelling the apoplastic fluid was incubated for 30 min with different concentrations of recombinant LpCys1 or chicken egg white cystatin (CEWC, Sigma-Aldrich, St. louis, Mississippi, USA) and Tris-HCl (24 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... the chamber was rinsed three times with T50 to remove non-specific interactions and a protocatechuic acid/recombinant protocatechuate-3,4-dioxygenase (Sigma Aldrich and Oriental Yeast Co., respectfully) (PCA/rPCD ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was probed with the indicated primary antibody (rabbit anti-GFP [1:1500, Abcam, #ab290], mouse anti-RFP [1:1000, Chromotek, #6G6], and mouse anti-2A [1:1000, Sigma, #3H4]), washed with TBST ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit polyclonal anti-FLAG (F7425, 1:200), mouse monoclonal anti-vinculin (V4505, 1:200), and mouse monoclonal α-SMA (A5228, 1:200) from Sigma; rabbit polyclonal anti–SOX9 (#82630 ...
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bioRxiv - Physiology 2020Quote: ... according to the manufacturer’s instructions (Mouse Leptin ELISA Kit, 90030; Crystal Chem, Zaandam, Netherlands; Rat/Mouse Insulin ELISA Kit, cat. EZRMI-13K; Merck Millipore). The intra- and interassay coefficients of variation (CVs ...
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bioRxiv - Microbiology 2021Quote: ... a blocking step of incubation with sheep serum for 1 hour was followed by immunostaining for N using a mouse monoclonal antibody (αN009 48) and detected using a sheep anti-mouse FITC conjugate (Sigma, UK). Phalloidin Alexa Fluor 568 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated with anti-mouse NeuN or anti-mouse parvalbumin (Millipore, MAB377, 1:100 and Sigma, P3088, 1:1000 respectively) overnight at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were blocked in Odyssey blocking buffer for 30 min at room temperature and incubated with primary antibodies (Mouse anti-V5 antibody, Invitrogen R960-25, 1:1000 dilution; Mouse anti-Flag antibody (Sigma F1804), 1:1000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... IF 1:500), mouse anti-cortactin (05-180, IF 1:400), mouse anti-talin (T3287, IF 1:200) all from Sigma-Aldrich; rabbit anti-EEA1 (ALX-210-239 ...
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bioRxiv - Cell Biology 2021Quote: ... or immunoprecipitating mAbs (mouse anti–cyclin B1 clone GNS3 or mouse anti–cyclin A clone E67.1; SC53230; Santa Cruz Biotechnology, Inc., or mouse IgG; Sigma-Aldrich) were added to the lysate (2 μg/ml cyclin B1 and 10 μg/ml cyclin A final concentration ...
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bioRxiv - Biochemistry 2022Quote: ... mouse anti-GBP130 (1:500), mouse anti-EXP2 (1:5000, 37), rabbit anti-Nluc (1:400, 65) and mouse anti-HA (1:500, Sigma-Aldrich). After primary antibody was removed ...
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bioRxiv - Biophysics 2022Quote: ... The α-tubulin antibody was revealed using Abberior STAR Red goat anti-mouse (Abberior) for the custom microscope or Alexa 546 goat anti-mouse (Sigma Aldrich) for the Nikon-based microscope ...
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bioRxiv - Microbiology 2021Quote: ... Biotinylated IFX fragments were immobilised on a streptavidin-coated plate and binding of the six mouse monoclonal antibodies was tested by ELISA and detected with an alkaline-phosphatase-conjugated anti-mouse secondary antibody (Sigma-Aldrich) as previously described43 ...
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bioRxiv - Immunology 2019Quote: ... the plates were washed with PBS four times and horseradish peroxidase-conjugated anti-mouse IgG or horseradish peroxidase-conjugated anti-mouse IgA (Sigma Aldrich) diluted 1 ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-βactin (AC-15, 1:20000) and mouse anti-FLAG (F-3165, 1:7000; IF 1:300) from Sigma-Aldrich; rabbit anti-TOP1 (A302-589A,1:5000) ...
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bioRxiv - Immunology 2020Quote: ... Mouse antibodies were detected by incubation with polyclonal goat anti-mouse IgG-horseradish peroxidase conjugate (Sigma-Aldrich, St. Louis, MO, USA). The plate was developed with TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualised using the Licor Odyssey CLx system with Licor secondary antibody (IRDye 800 CW anti-rabbit/mouse) or anti-mouse IgG (whole molecule) (A4416) or anti-rabbit IgG (Sigma-Aldrich) conjugated to horseradish peroxidase.
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bioRxiv - Neuroscience 2023Quote: ... which were blocked in 5% dry milk powder in TBS-T for 1 hour and then incubated in primary antibody solutions (Rabbit anti NUDT6, Proteintech 1:800; mouse anti FGF2, Millipore, 1:500; mouse anti ß-actin, Sigma, 1:7500 ...
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bioRxiv - Immunology 2023Quote: ... Antibody-stained cells were subsequently probed with Duolink In Situ PLA Probe anti-mouse PLUS and anti-mouse MINUS probes (Sigma/Merck). Next ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred onto an immobilon-FL membrane (Millipore) at 75 V for 1hr ...
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bioRxiv - Physiology 2021Quote: ... Proteins were transferred to a PVDF membrane (Merk Millipore), which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST ...
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bioRxiv - Biophysics 2021Quote: ... FnCas9 protein was purchased from Millipore Sigma (FNCAS9PROT-250UG).
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bioRxiv - Biophysics 2020Quote: Proteins were concentrated for structural studies using ultrafiltration (Sigma-Millipore ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were expressed in Rosetta 2 cells (EMD Millipore) grown in LB at 37°C and induced at an OD600 of 0.7 with 0.5 mM IPTG for 16 hours at 18°C ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were wet transferred onto PVDF membrane (Millipore, IPFL00010), blocked with 5% skimmed milk and probed successively with anti-Flag (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... beads containing bound proteins were incubated with thrombin (Novagen) overnight at 20°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to Immobilon-FL PVDF membrane (Millipore), and membranes were incubated in Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration in S3 was normalized via Bradford (SIGMA) to ensure that equal amount of protein was loaded before ultracentrifugation step ...
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bioRxiv - Molecular Biology 2022Quote: ... protein complex were eluted with 10mM Maltose (Sigma Aldrich). Resolve elutes by SDS-PAGE and transfer to PVDF membrane for western blots ...
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bioRxiv - Synthetic Biology 2022Quote: ... The protein sample was transferred to PVDF membranes (Millipore) using Mini Trans-Blot (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein estimation was performed using Bradford assay reagent (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... Antibodies were bound to protein G-Sepharose (Sigma-Aldrich) in RSB-100 buffer containing 0.1% NP40 ...
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bioRxiv - Molecular Biology 2021Quote: Protein mixtures were Trichloroacetic acid (Sigma Aldrich, Cat# T0699) - precipitated overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... protein bands were visualized with Ponceau stain (Sigma, P7170) to confirm protein transfer and even loading.
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were transferred to nitrocellulose membranes (Sigma #GE10600002). Western blot analysis was performed under standard conditions with antibodies against Tubulin (Sigma #T9026 ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred onto Immobilon-FL PVDF membranes (Millipore) and membranes were blotted with Anti-Flag M2 antibody (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... proteins were transferred to Nitrocellulose membranes (GE10600012, Sigma-Aldrich). Nitrocellulose membranes were blocked with 5% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... 75 μl pre-washed protein A sepharose (Sigma-Aldrich) was added for recovery of FLAG-antibody and incubated for 30 min ...