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Citations for Millipore Sigma :
4301 - 4350 of 10000+ citations for Recombinant Human ETS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration for lysates was determined using Bradford reagent (Sigma-Aldrich), loads were adjusted and proteins were resolved by SDS-PAGE and analyzed by western-blot (Western Lightning Plus-ECL kit ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... The crude protein lysate concentration was quantified by BSA (Sigma-Aldrich) and 25 μg crude extract was loaded on NuPAGE™ 3-8% Tris-Acetate gels (ThermoFisher ...
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bioRxiv - Immunology 2019Quote: ... these proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Millipore, # IPVH00010) and blocked with 5% skim milk (BD ...
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bioRxiv - Genetics 2021Quote: ... the proteins were blotted onto a PVDF membrane (Millipore, Billerica MA) and incubated with mouse monoclonal NQO1 (1:1000 ...
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bioRxiv - Genetics 2020Quote: ... proteins were transferred to Immunobilon-FL PVDF membranes (Fisher, Millipore, SLGV033RS) using NuPage transfer buffer transfer system overnight at constant 30 V ...
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bioRxiv - Genetics 2020Quote: ... Protein extraction and Western blot using anti-puromycin antibody (Merck Millipore) was performed as described before.
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bioRxiv - Genetics 2020Quote: ... or residues near the carboxylterminal end of the protein (ABN410, Millipore) with IR-700 conjugated goat anti-rabbit secondary antibody (Rockland 611130122 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were first reduced by incubation with dithiothreitol (5mM; Sigma-Aldrich) for 30 min at 60°C ...
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bioRxiv - Cell Biology 2021Quote: ... protein was purified by incubating with glutathione agarose (GST; Sigma Aldrich) or amylose resin (MBP ...
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bioRxiv - Physiology 2020Quote: ... as a carrier protein using 5% glutaraldehyde (Sigma-Aldrich, Gillingham, UK)in phosphate buffer (0.1 M ...
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bioRxiv - Molecular Biology 2021Quote: ... immunoprecipitated proteins were eluted using 3X-FLAG peptide (Sigma - catalog # F4799) for 2 hours at 4°C according to the manufacturer’s instructions.
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bioRxiv - Physiology 2020Quote: ... The protein concentration was determined by the Bradford reagent (Sigma-Aldrich). For each experiment ...
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bioRxiv - Neuroscience 2020Quote: ... Concentration of solubilized proteins was quantified using BCA assay (Sigma-aldrich). All samples were then set at the same volume and concentration for the rest of the protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were harvested using ice cold RIPA buffer (Millipore 20-188) supplemented with phosphatase (Sigma 4906845001 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred onto a PVDF Immobilon-P (0.45 µm Millipore), and SMN ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged proteins were purified using FLAG M2 affinity gel (Sigma) rotated end over end for 3 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and bound proteins were eluted with 3.5 mM d-desthiobiotin (Sigma) in the equilibration buffer (Besche and Goldberg ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were then transferred to a PVDF membrane (Merck Millipore) using a semi-dry western blot transfer system set to a constant current of 200 mA for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were pre-incubated with Protein- A Sepharose beads (Novagen) and allowed to mix for 30 min at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted proteins were concentrated using 30 kDa spin filters (Millipore) and further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Protein concentration in homogenates was determined using the BCA assay (Sigma).
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated in a 50,000 MWCO Amicon Concentrator (Millipore) to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... or 10 μM cytosolic protein synthesis inhibitor cycloheximide (CHX, Sigma-Aldrich) to induce mitochondrial fragmentation and hypertubulation ...
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bioRxiv - Cell Biology 2021Quote: ... Resolved proteins were transferred to PVDF membrane (0.45 μm, Millipore, #05317) followed by 1-hour incubation in a blocking buffer (TBS ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal Prosurfactant Protein C (1:200 pH9, AB3786, Merck Millipore), mouse monoclonal TTF-1 (clone SPT24 ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were visualized with chemiluminescence using ECL Lumina Forte (Millipore, France) and chemiluminescence detector (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-glial fibrillary acidic protein (GFAP, #G3893, Sigma-Aldrich).
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bioRxiv - Plant Biology 2022Quote: ... proteins were detected with alkaline phosphatase-conjugated antibody (Sigma-Aldrich A3687). Primary antibodies used were ...
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bioRxiv - Neuroscience 2021Quote: ... Protein bands were visualized using Immobilon Forte Western HRP Substrate (Millipore) and ChemiDoc system (BioRad).
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein concentration of the supernatant was determined by Bradford Reagent (Sigma). The proteins were then separated on a 12% SDS-PAGE (40 μg protein/lane) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred from gel to 0.45-micron PVDF membrane (Millipore) at 70 V for 2 h using wet-transfer in tris-glycine buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was eluted by digestion with Factor Xa protease (Sigma). Factor Xa protease was removed using p-Aminobenzamidine-agarose (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse GAPDH monoclonal antibody for protein loading controls (Sigma, G8795). Anti-mouse IRDye680RD and anti-rabbit IRDye800CW (Licor ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were transferred onto PVDF membranes (Immobilon; EMD millipore, Darmstadt, Germany) at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Cycloheximide (inhibitor of protein synthesis) was used at 10µg/mL (Sigma) and added 30 min prior to cytokine treatment of neutrophils.
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bioRxiv - Microbiology 2020Quote: ... We purified the protein using anti-FLAG M2 Affinity Gel (Sigma) according to manufacturer’s protocol and removed the FLAG tag and His6-tag with TEV digestion followed by reverse nickel affinity purification and sizeexclusion chromatography on a Superose 6 Increase column (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... Protein extraction was performed in the presence of 2% SDS (Sigma) and 4 M acetic acid (Fisher ...
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bioRxiv - Immunology 2020Quote: ... Proteins were transferred to Immobilon-FL PVDF transfer membrane (Millipore Sigma) using NuPAGE transfer buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... After transfer of the proteins onto a nitrocellulose membrane (GE10600001, Sigma) for 1 h at 100mV ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein bands were visualized with Immobilon Chemiluminescent Alkaline Phosphatase substrate (Millipore) and detected in a VersaDoc Model 4000 CCD camera system (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Bradford dye for protein estimation was purchased from Sigma-Aldrich (USA). All the primers were synthesized by GCC Biotech (India ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected with mouse anti-FLAG monoclonal (Sigma, 1/10000), rabbit anti-Rad51 polyclonal (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein was transferred to an Immobilon-P PVDF membrane (EMD Millipore). After transfer ...
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bioRxiv - Genetics 2021Quote: ... Proteins were then transfered to an Immobilon-E PVDF membrane (Millipore), which was then washed with 1× PBS containing 0.05% Tween 20 (three washes ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to a nitrocellulose membrane (Millipore, Bedford, MA, USA). Membranes were blocked with 5% non-fat dried milk in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were spiked with ubiquitin (Sigma-Aldrich BioUltra, 5 nmol), a small subunit protein native to eukaryotes ...
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bioRxiv - Biophysics 2021Quote: ... 2.5μL of Precision Plus Protein™ Dual Color Standard from Sigma was used.
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bioRxiv - Genetics 2019Quote: ... Proteins were transferred onto an Immobilon-P polyvinylidene difluoride membrane (Millipore) using a Bio-Rad Criterion Blotter ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were buffer exchanged in centrifugal concentrators (Amicon Ultra, EMD Millipore) to buffer A plus 300 mM NaCl and 20 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were then transferred to a nitrocellulose filter membrane (Millipore) and were incubated overnight with indicated primary antibodies ...