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Citations for Millipore Sigma :
4301 - 4350 of 4915 citations for Recombinant Human CTLA4 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... All-trans Retinoic Acid (RA) (R2625) and Brain-derived neurotrophic factor (BDNF human) (B3795) were purchased from Sigma Aldrich.
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bioRxiv - Immunology 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats by density gradient centrifugation using Hisopaque-1077 (Millipore) and SepMate PBMC isolation tubes (StemCell ...
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bioRxiv - Neuroscience 2023Quote: Protein lysates were collected from primary human and murine spinal cord astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: Human cell lines were grown at 37°C with 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, D6429) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNAs encoding human AMPKα1-KD (aa 27-290) (ref. 164) and rat CaMKK2-KD42 were inserted into the pET-28a (Novagen) vectors for expressing His-tagged recombinant proteins as described previously ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼30% CD90+ stromal cells were dissociated at 15 days using human cardiac digestion buffer: 0.2% collagenase type I (Sigma) in 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Phosphate buffered saline (PBS) containing 0.12% of DMSO and 4% of human serum albumin (HSA) (Sigma-Aldrich, MO, USA) was used as the negative control and incubation buffer ...
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bioRxiv - Immunology 2023Quote: Human Vγ9Vδ2 T cells were specifically expanded from fresh PBMCs ex vivo using 5 µM zoledronic acid (Sigma Aldrich) or 3 µM BrHPP (bromohydrin pyrophosphate ...
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bioRxiv - Microbiology 2023Quote: ... we added a calibration range by serially diluting a polyclonal IgG antibody from human sera solution (reference: I2511, Sigma), to reach concentrations ranging from 0.68 ng.mL-1 to 22 ng.mL-1 ...
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bioRxiv - Microbiology 2023Quote: ... Human liver hepatocellular carcinoma (HepG2) and Vero (African Green Monkey Kidney) cells (ATCC) were regularly maintained in DMEM (Sigma) supplemented with 10% FBS (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... human breast cancer (MCF7) and human embryonic kidney cells (HEK293T) cells were cultured in Dulbeco’s Modified Eagle Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum (Sigma) ...
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bioRxiv - Genetics 2023Quote: Human embryonic kidney (HEK) 293T cells were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS, Sigma), 1% penicillin-streptomycin (P/S ...
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bioRxiv - Microbiology 2023Quote: ... The degradation products of fibrinogen were detected by Western blot using rabbit anti-human fibrinogen polyclonal antibodies (Sigma-Aldrich) at a 1:5,000 dilution as described above.
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bioRxiv - Evolutionary Biology 2023Quote: ... GATA 3 (SAB2100898) (rabbit) 1:400 dilution for zebrafish tissue and 1:100 dilution for human tissue (Sigma Aldrich) and and CD44 (SAB1405590 ...
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bioRxiv - Pathology 2023Quote: Human or mouse lung tissue slides (7 μm) were fixed in 3.7% formalin (Sigma-Aldrich, St. Louis, MA, USA), permeabilized in 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... or a shRNA specific for human SOX11 (100% identity to the equine SOX11 sequence) (5’-CTGGTGGATAAGGATTTGGAT-3’ clone NM_003108.3-1235s1c1; Sigma-Aldrich) were created by transfection into HEK293T packaging cells ...
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bioRxiv - Genomics 2023Quote: The human pre-B ALL cell line Reh (DSMZ, ACC 22) was maintained in RPMI-1640 medium (Sigma, R0883) supplemented with 2 mM L-Glutamine (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Human plasma like media (HPLM) was generated according to the published formulation59 with addition of 5% dialyzed FBS (Sigma) and Pen/Strep (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Immunology 2023Quote: Lentiviral vectors containing validated shRNA sequences targeting expression of human PRKCD (accession NM_006254) or a non-target control were obtained from Sigma (catalog numbers TRCN0000010193 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell were then fixed as previously described and all three batches were permeabilised with Triton X-100 0.5% in PBS and a Cy5-conjugated anti-human (AC112S, Sigma) was used for the detection of T-DM1 and T-DXd ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were seeded onto plasticware coated with a solution of 0.1 % gelatin containing 10 µg/ml human plasma fibronectin (FN) (Millipore). and collagen type 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Normal human peripheral blood mononuclear cells (PBMCs) (FUJIFILM Wako Pure Chemical, Osaka, Japan) were cultured in RPMI1640 (Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: Human mini-colons cultured in biological triplicates were extracted from the PDMS chips and digested with collagenase I (Sigma) at 100 U/ml concentration in Advanced DMEM/F12 with penicillin/streptomycin ...
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bioRxiv - Microbiology 2024Quote: ... with 25 mM HEPES and (iii) RPMI with 25 mM HEPES and supplemented with 10% human serum (Sigma-H4522). Then ...
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bioRxiv - Cell Biology 2024Quote: ... The small interfering siRNA (siRNA) oligonucleotide targeting human FCHSD2 (5’-GCAUACUCCUGAGACCUCA[dT][dT]-3’) was obtained from Sigma Aldrich. FCHSD2 siRNA knockdown in all HeLa cell lines was performed for 48 h using the DharmaFECT transfection reagent (Dharmacon ...
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bioRxiv - Cell Biology 2024Quote: ... 3,3’ s-dihexyloxacarbocyanine iodine (DiOC6) and fibrinogen from human plasma were obtained from Sigma Aldrich (St. Louis, Missouri, USA). ML161 (PAR1 inhibitor ...
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bioRxiv - Genetics 2024Quote: ... cells were transferred into SFEM II supplemented with 3 U/mL hEPO (SCT) and 3% AB human serum (Sigma) for an additional 5 days of culture ...
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bioRxiv - Microbiology 2024Quote: Human neutrophils from the peripheral blood of healthy blood donors were isolated by density gradient centrifugation (Histopaque; Sigma–Aldrich) followed by hypotonic lysis of erythrocytes ...
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bioRxiv - Cell Biology 2024Quote: ... and human breast cancer cells (4175-LuT; Hoshino et al., 2015) were cultured in Dulbecco’s modified Eagle’s medium (Sigma‒ Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Human GBM commercial cell lines were maintained routinely in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma, New York, NY, USA), supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... The 3dGRO™ human ISO72 colorectal cancer tumoroid line was purchased from Millipore Sigma (Burlington, MA, USA, cat# SCC507) and cultured in vendor-specified homebrew media ...
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bioRxiv - Neuroscience 2024Quote: ... Then sections were washed in PB and 0.05 M Tris-buffered saline (pH 7.4; TBS) and blocked in 1% human serum albumin (Sigma-Aldrich) in TBS ...
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bioRxiv - Bioengineering 2024Quote: ... and glucagon were measured using a customized MILLIPLEX human metabolic Hormone Panel V3 (Millipore Sigma, cat. no. HMH3-34K). CCL2 ...
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bioRxiv - Cell Biology 2024Quote: Madin-Darby canine kidney cells with stable expression of human α-2,6-sialyltransferase (MDCK-SIAT1) (Sigma, catalog #: 05071502-1VL), human lung adenocarcinoma epithelial A549-Dual cells (InvivoGen ...
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bioRxiv - Cell Biology 2024Quote: ... human embryonic kidney HEK-293 T cells and human dermal fibroblasts (HDFs) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Sigma-Aldrich), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... The proximity ligation reaction and visualization of signal was performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for rabbit and human antibodies (Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Biochemistry 2024Quote: His6-tagged CCT domains (mouse SPAK 459-556 and human OSR1 433-527) and mouse SPAK 62-556 T243E were expressed in Rosetta (DE3) E.coli (Novagen). Cells were grown at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... T2 cells were incubated with a titration of each tested peptide and 100 ng/mL human β2-microglobulin (Sigma) in serum-free medium (TexMacs ...
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bioRxiv - Microbiology 2024Quote: ... Chips were coated overnight at 4°C with ECM composed of 200 ug/mL of human Collagen IV (Sigma) + 100 ug/mL of Matrigel (Corning) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1143-1300 of human RTEL1 were amplified by PCR then subcloned between the EcoRI and XhoI restriction sites of pET28 (Novagen). RTEL1 proteins were expressed as 6-histidine-fusions in the E ...
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bioRxiv - Immunology 2021Quote: ... MAb was reacted with antigen coated on the plates and then treated with serially diluted C1q from human serum (Sigma). C1q binding was detected with horseradish peroxidase-conjugated anti-C1q antibody ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 (human epithelial colorectal adenocarcinoma cell line) cells were purchased from ATCC and cultured in DMEM high glucose media (Sigma). The cells were trypsinized using 0.25% trypsin-EDTA and resuspended in the fresh media ...
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bioRxiv - Cell Biology 2020Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: The cDNA residues 1-169 of human Rheb (184 amino acid isoform, GenBank accession number EAW53989) was subcloned into the pET28a vector (Novagen), with an N-terminal 6×His-tag ...
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bioRxiv - Developmental Biology 2021Quote: Newborn pups were injected at P2 via the temporal vein with 0.0025mg/g of DLL4-neutralizing (blocking antibody (YW152F, Genentech (31)) or IgG from human serum (cat #I4506, Sigma Aldrich). The pups were sacrificed 48 h after injection at P4 ...
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bioRxiv - Molecular Biology 2021Quote: ... These wells were washed five times using 1× PBST and followed by addition 50 μl/well of anti-human IgG horseradish peroxidase (HRP) (Sigma) diluted in Stabilzyme Noble (Surmodics) ...
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bioRxiv - Pathology 2022Quote: ... biopsies of human xenograft harvested at the indicated time post infection were digested with 0.4 mg/ml Liberase TL (Sigma Aldrich) in CO2-independent medium for 60 min at 37°C with gentle agitation (100 rpm) ...
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bioRxiv - Cancer Biology 2022Quote: ... Metastases and micro-metastases were assessed using IHC with a human mitochondria antibody (Millipore MAB1273 Anti-Mitochondria clone 113-1).
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bioRxiv - Immunology 2019Quote: The sequence-encoding residues Val77–Leu233 of human TNFα was cloned and fused to the N-terminal 6xHis tag in pET-28a+ vector (Novagen) and transformed into Escherichia coli Rosetta (DE3 ...