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Citations for Millipore Sigma :
4301 - 4350 of 10000+ citations for P N Nonylphenol 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... OmniSolv® LC-MS grade acetonitrile and methanol were obtained from EMD Millipore (Billerica, MA). Tributylamine (TBA) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were chemically fixed with prewarmed (37°C) 4% methanol-free formaldehyde (Sigma-Aldrich, Germany) in PBS for 20 min followed by three washing steps with PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were placed into 1/3 Methanol and 2/3 dichloromethane (DCM, Sigma-Aldrich, 270997) with rotation at 13rpm at room temperature for overnight incubation ...
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bioRxiv - Biophysics 2024Quote: ... rinsed thoroughly in methanol in an ultrasonic bath then in a 2.5% glutaraldehyde (Sigma-Aldrich) solution in ddH20 for 45min and rinsed thoroughly in ddH20 in an ultrasonic bath then at room temperature in ddh20 overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were fixed by adding 40 µL of 37% formaldehyde (methanol-stabilized, Sigma-Aldrich #252549). For 96-well plates ...
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bioRxiv - Physiology 2024Quote: ... rinsed thoroughly in methanol in an ultrasonic bath then in a 2.5% glutaraldehyde (Sigma-Aldrich) solution in ddH20 for 45min and rinsed thoroughly in ddH20 in an ultrasonic bath then at room temperature overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... Metabolites were extracted in pre-cooled (-80°C) 80% HPLC grade methanol (Sigma-Aldrich #C34860). Metabolomic assays were performed either at the University of Pennsylvania Metabolomics Core ...
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bioRxiv - Biophysics 2022Quote: ... encoding CPB was cloned as N-terminal His6 or N-terminal GST fusion into pET19-b (Novagen) using NcoI and BamHI sites ...
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bioRxiv - Bioengineering 2019Quote: ... followed by addition of 1.12 g N-hydroxysuccinimide [9.72 mM] and 1.79 g of N-(3-(dimethylamino)propyl)-N’-ethylcarbodiimide hydrochloride [9.36 mM] (Sigma). The resulting solution was mixed at room temperature (RT °C ...
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bioRxiv - Cell Biology 2020Quote: ... macropinocytosis inhibitor 5-(N-Ethyl-N-isopropyl)amiloride (EIPA) from Sigma-Aldrich (A3085, used at 25 µM). Other ...
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bioRxiv - Neuroscience 2020Quote: ... Trim28-fl(+/− and +/+) animals (Ctl n=5, KO n=5) were put in RIPA buffer (Sigma-Aldrich) containing Protease inhibitor cocktail (PIC ...
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bioRxiv - Cell Biology 2021Quote: ... The dye solution is prepared dissolving the dye powder in anhydrous N,N-dimethylformamide (DMF, Sigma-Aldrich). The dye to protein molar ratio was adjusted between 2 - 5 in order to provide a DOL around 1 ...
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bioRxiv - Bioengineering 2022Quote: ... Polymerization of 1 M monomer was performed with 4 mol% N,N’-methylene-bis-acrylamide (Sigma-Aldrich) as a crosslinker with 0.1% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... Evans blue dye was extracted from the organs by the addition of N,N-dimethylformamide (Sigma-Aldrich) (adjusted to 4 mL/g wet tissue ...
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bioRxiv - Biophysics 2020Quote: Unsized unilamellar DOPG liposomes containing Laurdan (6-Dodecanoyl-N, N-dimethyl-2-naphthylamine, from Sigma, Taufkirchen, Germany) (molar ratio lipid:Laurdan=1:500 ...
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bioRxiv - Microbiology 2020Quote: ... We then prepared a solution of 457 mM N,N-Bis(carboxymethyl)-L-Lysine-hydrate (Sigma-Aldrich) in 1 M NaHCO3 (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2019Quote: ... To evaluate the inhibitory effect of macropinocytic inhibitor 5-(N-ethyl-N-isopropyl) amiloride (EIPA, Sigma-Aldrich) on the uptake of nanoparticles ...
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bioRxiv - Developmental Biology 2020Quote: ... and N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT D5942, 1μM, Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... with the addition of N-methyl-N-t-butyldimethylsilyltri-fluoroacetamide (MTBSTFA) (Sigma-Aldrich, St. Louis, MO, USA) (Richardson ...
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bioRxiv - Biochemistry 2022Quote: ... Methoxaymine hydrochloride (#226904, MOX) and N-methyl-N-(trimethylsilyl) trifluoroacetamide (#694709, MSTFA) were purchased from Sigma Aldrich. Protease inhibitor cocktail (#786-437 ...
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bioRxiv - Neuroscience 2022Quote: ... The interior walls of the capillaries were silanized with silan vapors (N,N-Dimethyltrimethylsilylamine, Sigma Aldrich, France) for 15 min and dried at 200°C for 100 min ...
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bioRxiv - Biophysics 2024Quote: ... acrylic acid (AAc) and cross-linker N,N’-methylenebisacrylamide (BIS) (all Sigma-Aldrich, A9099, 147230, 146072, respectively) with a final pH of 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... at 1/1000 dilution or Laurdan (#40227, 6-Dodecanoyl-N,N-dimethyl-2-naphthylamine, Merck, Sigma-Aldrich) at 1/500 dilution for 30 min at 37°C in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue slides were mounted in glycerol / n-propyl gallate medium (4% n-propyl gallate (02370, Sigma-Aldrich), 8 ml 100% glycerol ...
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bioRxiv - Bioengineering 2023Quote: ... fully dissolved in a solvent system of 60/40 volume ratio of N,N-dimethylformamide (DMF) (Sigma) to acetone (Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... Evans Blue was extracted from tissue pieces for 48 hours in N,N-Dimethylformamide (Sigma, cat. #D4551) at 55°C ...
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bioRxiv - Cancer Biology 2020Quote: ... The equivalent of 5 mg of protein was further enriched for phosphorylated tyrosine peptides by overnight immunoprecipitation (pY-IP) using a cocktail of anti-phosphotyrosine antibodies (4G10, Millipore; PT-66, Sigma;p-Tyr-100, Cell Signaling Technology).
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bioRxiv - Neuroscience 2023Quote: Cultured cells and hMLOs were washed with PBS and lysed with protein lysis buffer (100 mM NaCl, 1.0% Nonidet P-40 Substitute [Sigma, 74385], 50 mM Tris-Cl, pH 7.4) supplemented with protease inhibitors Complete® (Merck ...
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bioRxiv - Neuroscience 2023Quote: Cultured cells were washed with PBS and lysed with protein lysis buffer (100 mM NaCl, 1.0% Nonidet P-40 Substitute [Sigma, 74385], 50 mM Tris-Cl, pH 7.4) supplemented with protease inhibitors Complete® (Merck ...
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bioRxiv - Cell Biology 2020Quote: ... On day 9 medium was supplemented with FGF8b (100 ng/ml, R&D) and heparin (1 μg/ml, Sigma). On day 11 cells were lifted using Accutase (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... the cells are mixed with Cmp (34 μg/mL), Amp (100 μg/mL) and Pluronic F127 (0.001%, to prevent cells adhering to the PDMS surface31) (Sigma) in LB to the density of 108 cells/mL ...
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bioRxiv - Biophysics 2021Quote: ... Purified hVLPs were brought up to 15 ml with PBS and concentrated to ∼0.2 ml using a 100 kDa MWCO Amicon centrifugal filter (Millipore). VLPs were inactivated by adding aldrithiol-2 (AT-2 ...
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bioRxiv - Neuroscience 2020Quote: ... We dissolved tamoxifen (100 mg) with 1 ml of ethanol and 9 ml of sunflower seed oil (Sigma-Aldrich). At the age of eight weeks ...
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bioRxiv - Biophysics 2020Quote: ... The protein was eluted from the column with Wash Buffer supplemented with 3 mg mL-1 1D4 peptide and concentrated to 4.3 mg mL-1 using a 100-kDa concentrator (Millipore) before further purifying by size exclusion chromatography using a Superose 6 increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... The peak fractions containing fPanx1ΔC were collected and concentrated to ∼0.2 mg ml-1 using a 0.5 ml concentrator with 100 kDa cut-off (EMD Millipore), for negative-stain EM and cryoEM ...
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bioRxiv - Biochemistry 2022Quote: ... and concentrated to around 2 mg/ml using Amicon Ultra 0.5 ml-100 kDa cutoff (Millipore, Burlington, US-MA).
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bioRxiv - Plant Biology 2022Quote: Col-0 and Col-0::SpCas9 seeds were surface sterilized by placing in a 1.5 mL sterile tube and 1 ml 75% ethanol and 0.1% Triton X-100 (Sigma-Aldrich) was added and placed in Eppendorf Mixer 5432 for 4-5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... remaining tissue was then minced and digested by Collagenase IV (0.1 g/100 mL, sigma) and DNase I (150 U/mL, Sigma) for 40 min at 37°C whilst being gently shaken ...
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bioRxiv - Microbiology 2019Quote: ... Water-free ethanol was prepared by adding 2 ml acidulated 2,2-dimethoxypropan (two drops of 37% HCl ad 100 ml 2,2-dimethoxypropan (Sigma Aldrich)) to 100 ml ethanol absolute (Merck) ...
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bioRxiv - Genomics 2022Quote: ... Cells were first washed two times on ice with 10 ml of PBS containing 100 μg/mL cycloheximide (Sigma). Cells were lysed in 50 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... lung cells were pre-activated with 100 ng/mL of PMA and 1 µg/mL of ionomycin (Sigma-Aldrich) for 3 hrs in the 37°C before CD40L surface staining ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM magnesium chloride pH 7.4) and incubated overnight in 100 ml reaction buffer (kinase buffer + 0.2 mg/ml BSA (BSA Fraction V, Sigma) + 50 μg/ml kanamycin) ...
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bioRxiv - Microbiology 2024Quote: ... or 100 µg/ml INH (prepared from a 50 mg/ml stock in water, Cat#I3377, Sigma, Livonia, MI). Mtb samples obtained from log-phase liquid culture ...
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bioRxiv - Immunology 2023Quote: ... intracellular cytokine production was analyzed after restimulation with 100 ng/mL phorbol myristate acetate (PMA) and 1μg/mL ionomycin (both Sigma) for a total 6h ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 2004) and incubated overnight in 100 ml reaction buffer (kinase buffer + 0.2 mg/ml BSA (BSA Fraction V, Sigma) + 50 µg/ml kanamycin) ...
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bioRxiv - Cell Biology 2023Quote: ... and FLAG-Sox4 protein was eluted by 5 rounds of competitive elution with 1× column volume (0.6 ml) of 100 μg/ml FLAG peptide (Sigma) in TBS ...
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bioRxiv - Developmental Biology 2023Quote: ... then medium was changed to IMDM +0.1% BSA +/- TNF-α (100 pg/mL or 10 ng/mL; Sigma-Aldrich) and incubated at 37°C 5% CO2 for 6-24hrs ...
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bioRxiv - Genetics 2023Quote: ... and digested in 20 mL of DMEM added with 20% FCS and 100 U/ml of collagenase (Sigma-Aldrich) for 40 min at 37 °C ...
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bioRxiv - Biophysics 2024Quote: ... 500 mL cultures were resuspended in 100 mL of hypertonic sucrose solution containing lysostaphin (Sigma-Aldrich, St. Louis, MO) for cell wall digestion (0.5 M Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... color reactions were developed with p-nitrophenyl phosphate (1 mg/ml in Tris-HCl with 1 mM MgCl2) (Sigma-Aldrich, St. Louis, MO). Plates were read using 2300 EnSpire Multilabel Reader (PerkinElmer ...