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Citations for Millipore Sigma :
4201 - 4250 of 10000+ citations for Recombinant Human IL18 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred to an Immobilon-P PVDF membrane (EMD Millipore). Primary and secondary antibodies were added in order ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were transferred to an immobilon-P membrane (EMD Millipore). For protein detection ...
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bioRxiv - Cell Biology 2021Quote: ... The tagged proteins were immunoprecipitated using FLAG M2 antibody beads (Sigma). Co-precipitating RNAs were reverse transcribed using random hexamers and Superscript III (Life Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... protein was expressed in N5052 minimal medium24 using 15NH4Cl (Sigma-Aldrich). For 13C,15N labelling ...
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bioRxiv - Biophysics 2020Quote: ... resolved proteins were transferred onto Immobilon-P PVDF membranes (Millipore Sigma) using a Bio-Rad Trans-blot® semi-dry transfer cell ...
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bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were expressed via BL21(DE3) Rosetta Escherichia coli cells (Novagen). Full-length PRC1 was expressed for 3.5 hours at 18°C after induction with 0.5 mM IPTG ...
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bioRxiv - Biophysics 2020Quote: ... Eluted protein was concentrated using 100-kDa cut off concentrator (Millipore) and further purified by Superose 6 increase (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysate protein bands were normalized to GAPDH (EMD Millipore, MAB347) at 1:2000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... Resolved protein was transferred to PVDF membranes (Millipore, Billerica, MA, USA) using a Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Darmstadt, Germany) at 14V constant in a semi-dry transfer machine ...
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bioRxiv - Cell Biology 2020Quote: Proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore) using wet transfer ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were electroblotted to 0.45μm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010 ...
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bioRxiv - Cell Biology 2021Quote: ... the electrophoresed proteins were transferred from gel to PVDF membranes (Millipore) following with incubation with primary and secondary antibodies and finally bands were detected ...
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bioRxiv - Cell Biology 2021Quote: ... serine/threonine protein phosphatase inhibitors (Phosphatase inhibitor Cocktail 3, Sigma-Aldrich) and 1 mmol/L Na3VO4 ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-Collapsin Response-Mediated Protein 5 (CRMP5, 1:50, Millipore), mouse anti-γ-tubulin (1:50 ...
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bioRxiv - Cell Biology 2021Quote: ... Total proteins were stained with Ponceau S solution (P7170, Sigma-Aldrich). The membrane was blocked with tris-buffered saline containing 3% bovine serum albumin (cat ...
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bioRxiv - Biochemistry 2022Quote: ... After probing for total transferred protein using Ponceau S (Sigma-Aldrich), membranes were blocked overnight in Odyssey Blocking Buffer (LICOR ...
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bioRxiv - Cell Biology 2022Quote: ... protein was extracted in 1x Radioimmunoprecipitation (RIPA) lysis buffer (Millipore Sigma) and then quantified using a bicinchoninic acid protein assay (BCA ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were concentrated with 30-kDa-cutoff Amicon centrifuge filters (Millipore), flash-frozen in liquid nitrogen and stored at -80°C ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... Protein extraction buffers contained 50mM HEPES (Sigma Aldrich, H0887-100 ml) and 150 mM NaCl ...
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bioRxiv - Physiology 2022Quote: ... Protein bands were visualized by chemiluminescent HRP substrate (WBKLS0500, EMD Millipore). Blots were developed with autoradiography films (HyBlot CL ...
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bioRxiv - Plant Biology 2022Quote: ... The proteins were then transferred to PVDF membranes (Immobilon-P; Millipore) and subsequently blocked with 5 % nonfat dry milk for 2 hours ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... The proteins were transferred to a polyvinylidene fluoride membrane (PVDF, Millipore) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Cell pellets were then resuspended in RIPA protein extraction buffer (Sigma) supplemented with protease inhibitor (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... and protein concentration was measured with BCA assay (Sigma 71285-M). Then ...
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bioRxiv - Molecular Biology 2022Quote: ... and proteins were precipitated by the addition of trichloroacetic acid (TCA_SIGMA) to a final concentration of 18% ...
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bioRxiv - Immunology 2022Quote: ... or 100 µg protein + 10 µg/mL MG132 (Sigma catalog # 474787). Antigen pulses with and without inhibitors were performed for 6 hr at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... the proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, USA) that were subjected to blocking by 5% skimmed milk for 1 h at room temperature ...
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bioRxiv - Plant Biology 2022Quote: Heterologous protein expression was achieved using pET28b(+) (EMD Millipore, Burlington, MA), in which open reading frames for the enzymes were cloned into using doubly digested vectors of either BamHI/XhoI (ASAT4H) ...
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bioRxiv - Biophysics 2022Quote: ... and mouse monoclonal primary antibody against myelin basic protein antibody (Millipore) at 1:200 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were treated with the protein synthesis inhibitor cycloheximide (Sigma) for the indicated durations before harvesting and analysis by immunoblotting.
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bioRxiv - Cell Biology 2022Quote: ... 0.5% BSA) and incubated with protein G beads (Sigma, 16-662) and antibody overnight at 4 °C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred from gels onto PVDF membrane (EMD Millipore, IPFL00010) using the Genscript eBlot L1 wet transfer system (cat# L00686) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then transferred to polyvinyledene difluoride (PVDF) membranes (Millipore IPVH00010) in Phos-tag transfer buffer at 20V for 20 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then transferred onto polyvinylidene difluoride membranes (EMD Millipore). The membrane was blocked with PBS-0.05% Tween 20 (PBS-Tween ...
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bioRxiv - Immunology 2020Quote: ... Proteins were detected by using enhanced chemiluminescence (ECL) systems from Millipore or GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were visualized after transfer to Immobilon-FL PVDF membrane (Millipore). Membranes were blocked with Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The proteins were then alkylated with 50 mM iodoacetamide (Sigma Aldrich) for 30 min in the dark ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Total protein was detected with Ponceau-S solution (Sigma-Aldrich, #P3504) or with stain-free technology (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were precleared with protein G Affinity Gel (Sigma-Aldrich #E3403) for 1 hr on rotator at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Protein was extracted from brain homogenates in RIPA buffer (Sigma, UK) containing cOmplete™ Mini Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein synthesis was measured using a DHFR assay kit (Sigma-Aldrich), which measures the oxidation of NADPH (60 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins digest were carried out with 0.75 µg of trypsin (SIGMA) for 16 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were then transferred onto a polyvinylidene fluoride (PVDF) membrane (Millipore). After blocking with 5% milk solution ...
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bioRxiv - Microbiology 2021Quote: ... The transferred proteins were stained with Ponceau S solution (Sigma-Aldrich). The nitrocellulose membrane was soaked (blocked ...
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bioRxiv - Biophysics 2020Quote: ... Protein fractions were pooled and concentrated (10.000 MWCO Amicon; Merck-Millipore) up to 5 mL and analyzed on a HiLoad 26/60 Superdex 200 (GE ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to polyvinylidinefluoride membranes (Millipore Corporation, Billerica, MA, USA), subsequently blocked 5% dry milk in TBST (Tris Buffered Saline with 0.1% Tween 20 ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by addition of Protein-A agarose beads (Sigma-Aldrich #1719408001) for 1 h at 4°C ...
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bioRxiv - Immunology 2020Quote: Total proteins were extracted by RIPA Buffer (R0278, Sigma-Aldrich, USA) containing protease inhibitors (HY-K0010 ...