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Citations for Millipore Sigma :
4201 - 4250 of 10000+ citations for IL 3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: The human megakaryoblast leukemic cell line MEG-01 (Sigma-Aldrich, ECACC 94012401) was maintained in RPMI 1640 Medium + GlutaMAX™-I (Gibco™ – Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Fresh citrate-anticoagulated human blood was pre-labeled with Mepacrine (Sigma-Aldrich) for 10 min at RT and perfused over the pre-coated coverslips using a flow chamber system (50 µm x 5 mm ...
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bioRxiv - Neuroscience 2023Quote: ... mouse antibody specific for human SOD1 (SD-G6 1:100, Millipore Sigma), Mouse anti-human HSP70 specific for stress-inducible HSPA1A (SMC-100B 1:100 ...
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bioRxiv - Immunology 2023Quote: ... followed by injection of 6.5 U human chorionic gonadotropin (hCG; Sigma-Aldrich) 48 h later ...
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bioRxiv - Cell Biology 2024Quote: Human retinal pigment epithelial (RPE) cells were cultured in DMEM (Sigma, #D5648) supplemented with 10% fetal bovine serum and maintained at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... and (2) the Human Cytokine 23-Plex Discovery Assay® (HD23; Millipore MILLIPLEX® Human Cytokine/Chemokine Magnetic Bead Panel II Immunology Multiplex Assay ...
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bioRxiv - Microbiology 2024Quote: ... and then incubated with either (1) 20 µg human fibronectin (EMD Millipore); (2 ...
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bioRxiv - Immunology 2024Quote: ... 5 distinct shRNAs directed against CD2AP encoding human CMS (shCMS) (Sigma-Aldrich Mission TRCN shRNA Target set ...
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bioRxiv - Synthetic Biology 2024Quote: ... or 96-well plates pretreated with 20 µg/mL human fibronectin (Millipore) for at least 10 min at 37°C.
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bioRxiv - Synthetic Biology 2024Quote: ... or 24-well plates pretreated with 20 µg/mL human fibronectin (Millipore) for at least 10 min at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... human full length NHE1 was cloned into p3xFLAG-CMV-14 (Sigma-Aldrich) containing a C-terminal 3xFLAG tag ...
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bioRxiv - Cell Biology 2022Quote: ... Growth medium was then removed from apical side and on the basolateral side it was replaced by ALI differentiation medium +/- 10 ng/ml IL-13 (IL012; Merck Millipore). Once the ALI interface was established medium was exchanged every second day till day 25-28 on ALI ...
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bioRxiv - Microbiology 2020Quote: ... the apical medium was removed and basolateral medium was switched to differentiation medium ± 10 ng/ml IL-13 (IL012; Merck Millipore). Differentiation medium consisted of a 50:50 mixture of DMEM-H and LHC Basal (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... 106 cells/ml in 500ul of regular medium supplemented with IL-2 500UI in presence of protamine-sulfate at 20ug/ml (Sigma). The plates were then centrifuged at 1,000 g for 1 h and incubated at 37°C overnight ...
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bioRxiv - Immunology 2022Quote: ... Transient expression of human IL-18BP or IL-18BPΔNΔN was achieved using branched polyethyleneimine (PEI) (Mn ∼ 10 000, Cat.:40,872-7, Sigma-Aldrich, Diegem, Belgium) as transfection reagent (67) ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml recombinant IL-1β (Preprotech) or 100 ng/ml lipopolysaccharide from Escherichia coli O55:B5 (LPS, Sigma-Aldrich) for 6h at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... We therefore investigated the bioactive LM profiles in tendon-derived stromal cells isolated from the tendons of healthy volunteers and patients in the presence of IL-1β (10 ngmL-1, Sigma) in medium (DMEM F12 phenol red free medium ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) was added ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) was added ...
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bioRxiv - Microbiology 2022Quote: ... according to the manufacturer’s instructions and activated by incubation in the presence of 100 U/ml IL-2 (Sigma Aldrich) and T Cell TransAct™ human (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: BMDM were polarized or not with recombinant IL-4 (10 ng mL−1, peprotech) for 24 hours and with LPS (10 ng/ml, Sigma) for 8 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 and 7 post IL-12mRNA/scRNA injection (day 11, 13 and 17 post inoculation) and digested in 30% collagenase (Sigma) for 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in the presence of recombinant mouse IL-4 (25 ng/mL; #404-ML, R&D) and LPS (20 μg/mL; #L2880, Sigma) over night to induce B cell activation and terminal differentiation ...
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bioRxiv - Immunology 2023Quote: ... Sorted Treg cultures also contained 1000 U/mL of IL-2 (Proleukin) and 50 nmol/L of rapamycin (Sigma- Aldrich), whereas Tconv cultures contained 100 U/mL of IL-2 ...
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bioRxiv - Neuroscience 2023Quote: ... and reconstituted to 1 mg/mL in sterile water and diluted to 250 μg/mL (IL-9) or 10 μg/mL (IFNγ and IP-10) in 0.1% cell-culture grade bovine serum albumin (Sigma A9418) in 1X PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured in 50µL of IL-11 and SCF media2 for 2h before being supplemented with polybrene (Sigma-Aldrich) and up to 20uL of a lentivirus equipped with the gene of interest (STAT5B-WT or STAT5B-Y699F ...
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bioRxiv - Bioengineering 2024Quote: ... the hydrogel- containing cells were maintained in RoosterBasal™ medium and treated with 1 ng/mL of IL-1β (Sigma) for 4 days at 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... and product intensity measured by Software ImageJ(83) and normalized to ACTIN amplicon products (5’-GACGACATGGAGAAAATCTG-3’ and 5’-ATGATCTGGGTCATCTTCTC-3’, Sigma-Aldrich, St. Louis, Missouri, USA).
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bioRxiv - Neuroscience 2021Quote: ... FW 5’ – CCA CAT GGG AGA GTC ACA T −3’ and RV 5’-ATA GCC TGG AAG CGG TCA GAT G −3’ (Sigma-Aldrich Japan, Inc., Tokyo, Japan). The PCR products (521 bp ...
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bioRxiv - Bioengineering 2022Quote: ... crosslinking using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Carl Roth GmbH + Co. KG) and N-hydroxysuccinimide (NHS) (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) was applied ...
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bioRxiv - Microbiology 2022Quote: The AHL signaling molecules used in this study are N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL, Sigma-Aldrich CAS# 168982-69-2), N-butyryl-homoserine lactone (C4-HSL ...
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bioRxiv - Neuroscience 2022Quote: ... BrdU injections were done 24h before sacrifice for the analysis at 3 dpKA and 3/7 days post administration of zinc (Cat#83265-250ML-F, Sigma-Aldrich, St Louis, MO, USA). To identify newborn cells at 14 dpKA ...
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bioRxiv - Plant Biology 2024Quote: ... 2-(1H-Indol-3-yl)-4-oxo-4-phenyl-butyric acid (PEO-IAA; OlChemIm, Olomouc, Czech Republic) and indole-3-carbinol (I3C; Sigma-Aldrich, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Neuroscience 2023Quote: ... The sections were then dehydrated in ascending concentrations of ethanol for 5 min each (2[×[35%, 50%, 70%, 80%, 90%, 3[×[100%) and washed 3 times for 5 min with propylene oxide (Sigma-Aldrich, #cat 110205-18L-C). Next ...
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bioRxiv - Genetics 2021Quote: ... Anti-His (SCBT, #sc-8036; used in 1:2000), anti-GST (SCBT, #459; used in 1:5000) and anti-Flag (Sigma-Aldrich, #F7425;used in 1:2000). Peroxidase conjugated anti-mouse (Jackson Immunoresearch ...
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bioRxiv - Cell Biology 2019Quote: ... Protein eluates were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto a nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Cell Biology 2019Quote: ... The proteins of the eluate were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Microbiology 2020Quote: ... Smears were blocked in 3% bovine serum albumin (BSA; Sigma-aldrich). Slides were incubated with primary rat anti-HA (Roche ...
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bioRxiv - Biophysics 2020Quote: ... chips were incubated in 2% APTES (3-aminopropyl-triethoxysilane) (Sigma Aldrich) in anhydrous toluene (Alfa Aesar ...
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bioRxiv - Cancer Biology 2021Quote: ... Sesamol and 3′,4′-(Methylenedioxy) acetophenone were purchased from Sigma-Aldrich. All the analytical grade chemicals were procured from Merck Limited ...
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bioRxiv - Cell Biology 2020Quote: ... serum-deprived Calu-3 were loaded with 10μM DCF (Sigma D6883) in low-serum media for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... (3) LPS with rottlerin (dissolved in DMSO, Sigma Aldrich, Cat #: D4540) treatment (10 µg/mL LPS and 1 μM rottlerin ...
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bioRxiv - Cell Biology 2020Quote: ... GAPDH (glyceraldehyde-3-phosphate-dehydrogenase, Sigma Aldrich, G8795, dilution 1:7500), CV (ATP-Synthase ...
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bioRxiv - Cell Biology 2020Quote: MTT (3- [4,5-dimethyl-2-thiazolyl] −2,5-diphenyltetrazolium bromide, Sigma M5655) is a tetrazolium salt giving a yellow solution when diluted in the medium ...
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bioRxiv - Cell Biology 2020Quote: ... followed by treatment with 500 µM auxin (3-Indoleacetic acid; Sigma) for 5-6 hrs.
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bioRxiv - Cell Biology 2020Quote: ... and 0.5 mM of 3-isobutyl-1-methylxanthine (IBMX) (Sigma, I5879). After three days ...
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bioRxiv - Cell Biology 2020Quote: ... The surfaces were then incubated with 5% (3-Aminopropyl)trimethoxysilane (Sigma) in ethanol for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... concentrated using Centrifugal Filter Units (Amicon Ultra-15 3 kDa) (Millipore), and subjected to gel filtration chromatography using a Hiload 16/60 Superdex 75 gel filtration column (GE Healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate (Sigma-Aldrich, 136149) in [0.1 M Tris-HCl ...
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bioRxiv - Microbiology 2019Quote: ... for 3 hours and then treated with nigericin (20 μM; Sigma) for 45 minutes ...