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Citations for Millipore Sigma :
4201 - 4250 of 10000+ citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: The HSMPs were synthesized by mixing 500□μL of 2□μm spherical beads based on polystyrene (PS) (Sigma-Aldrich cat. no. 78452), 1□mL ethanol 99% (Panreac Applichem cat ...
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bioRxiv - Cell Biology 2021Quote: ... The GST-Cdc14 constructs were expressed at 16°C overnight and the protein purification followed the same procedure as for 6His-3Flag-Cdc14 using glutathione-Sepharose beads (Millipore-Sigma, 17075601) and the following buffers ...
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bioRxiv - Neuroscience 2020Quote: ... and after the second (GD 44) and third (GD 46) Poly ICLC injections using a nonhuman primate multiplexing bead immunoassay (Milipore-Sigma, Burlington, MA) that was analyzed using the flow-based Luminex™ 100 suspension array system (Bio-Plex 200 ...
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bioRxiv - Microbiology 2020Quote: ... solution A from the kit and the supernatant was transferred into a new tube with one small spoon of glass beads (425-600 um, acid washed) (Sigma-Aldrich, USA). The samples were then vortexed three times in intervals of 3 seconds to break the cells ...
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bioRxiv - Microbiology 2019Quote: ... For metabolite extraction 1 ml 80% methanol containing 10 µM ribitol (RI, internal standard) were added to dried samples and for cell disruption 500 mg acid-washed glass beads (Sigma-Aldrich, USA) and a homogenizer (Precellys ...
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bioRxiv - Biophysics 2020Quote: ... The DNA construct is attached to the streptavidin coated beads by incubating 0.5 μl of picomolar DNA stock solution and 2 μl beads in 250 μl 1x phosphate buffered saline (PBS, Sigma-Aldrich, USA) for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... Bound beads were loaded onto a Bio Spin® Chromatography Column and washed with lysis buffer containing 20mM imidazole (Sigma-Aldrich, I0250) and eluted with lysis buffer containing 300mM imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... Essentially a colony of yeast is resuspended in 250ul of P1 buffer combined with 100 μl of acid-washed glass beads (Sigma G-8772) and vortexed for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... and precleared using protein A agarose beads (25%, diluted in ChIP dilution buffer; Protein A Agarose/Salmon Sperm DNA, Millipore, 16-157) and incubated overnight at 4°C (final buffer composition during antibody incubation was 0.1% SDS ...
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bioRxiv - Genetics 2022Quote: ... The aqueous phase was transferred to a fresh tube containing 200 µL of bead buffer and 400 µL of phenol/chloroform/isoamyl alcohol (25:24:1, Sigma-Aldrich, USA). Tubes were stirred briefly and centrifuged at 4 °C ...
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bioRxiv - Molecular Biology 2019Quote: The nucleic acid binding property of rcCBP2 was further validated using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 3µg of rcCBP2 was mixed with 500 µl cellulose matrix [1 mg/ml matrix prepared in 50 mM sodium phosphate (pH 7.6) ...
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bioRxiv - Immunology 2019Quote: ... for 6 hours and then exposed to 0.5 × 105 red and 0.5 × 105 blue opsinised (1 hour in 10%v/v human serum) 3 μm diameter latex polystyrene beads (Sigma-Aldrich, Gilligham, UK) such that there were 1 bead per cell ...
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bioRxiv - Microbiology 2019Quote: ... The beads were resuspended in 100 μl of NP‐T buffer containing 1 mM MgCl2 and 50 units of Benzonase (Sigma; cat # E1014) and incubated for 10 min at 37°C in a heat block ...
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bioRxiv - Physiology 2020Quote: ... Healthy animals were then transferred to a 96-well plate in a container filled with silica gel beads (Sigma-Aldrich, MO, USA) to produce a low humidity environment (app ...
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bioRxiv - Biochemistry 2021Quote: ... Sepharose CL-4B beads (50 μL of 50% slurry) was aliquoted onto a well of a MultiScreen Solvinert filter plate (Millipore, Billerica, MA), conditioned with water (200 μL) ...
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bioRxiv - Microbiology 2020Quote: ... The beads were resuspended in 100 μl of NP‐T buffer containing 1 mM MgCl2 and 50 units of Benzonase (Sigma; cat # E1014) and incubated for 10 min at 37°C in a heat block ...
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bioRxiv - Microbiology 2021Quote: ... A percentage of the cleared lysates was reserved for analysis and the remainder incubated with FLAG M2 affinity beads (Sigma, cat# A2220) overnight and washed 5X with WCEB ...
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bioRxiv - Immunology 2020Quote: ... Env protein bound beads were further incubated with blocking buffer (PBS [pH 7.4], 0.1% bovine serum albumin [BSA; Sigma], and 0.05% Tween 20) at 37°C to block the unbound sites and the antigenic integrity of Env proteins were assessed by examining their ability to bind to different mAbs by flow cytometry (FACSCanto ...
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bioRxiv - Cell Biology 2022Quote: ... mCherry tagged Drosophila Fab1 complexes with anti-mCherry antibody were precipitated by ∼60 µl slurry of washed and blocked protein-G sepharose beads (according to manufacturer’s protocol, Sigma # GE17-0886-01) for 2 hours at 4 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The samples were ground with two sizes of glass beads (20 μL volume each of ‘≤106 μm’ and ‘425–600 μm’) (Sigma-Aldrich, Merck Millipore, Germany) using a vortex and performing 5 cycles of freezing and thawing with a −80°C freezer ...
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bioRxiv - Systems Biology 2022Quote: ... Healthy animals were then transferred to a 96-well plate in a container filled with silica gel beads (Sigma-Aldrich, MO, USA) to produce a low-humidity environment (approx ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from yeast cultivations with ‘GC Prep’ (Blount et al. 2016) using 425-600 μm acid-washed glass beads (Sigma-Aldrich, USA) and a 5% suspension of Chelex® 100 sodium form as particle size 50-100 mesh (dry ...
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bioRxiv - Molecular Biology 2022Quote: The nucleic acid binding property of protein was further validated using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 1 μgm recombinant protein was mixed with 500 µl cellulose matrix (1mg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA/DNA and alpha/beta tubulin were tested using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 1 µg of PfCoSP was mixed with 500 µl cellulose matrix (1mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... vesicular fraction from brains obtained as described earlier was first pre-cleared for an hour at 4°C with protein A Sepharose beads (Sigma Aldrich-P9424) and then immunoprecipitated for 3 hours at 4°C by agarose beads preincubated with rabbit anti-HTT D7F7 antibody (Cell Signaling ...
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bioRxiv - Molecular Biology 2024Quote: ... The barcoded hydrogel beads were suspended in 1x First Strand buffer (TFS, 18080044) supplemented with 0.3% (v/v) IGEPAL CA-630 (Sigma-Aldrich, 18896-50ML). The 2x RT-lysis mixture for inDrops-1 protocol comprised ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were lysed with NP-40 lysis buffer and lysates and bead fractions were collected and treated for 10 min with Benzonase (Sigma, E1014-25KU) to break up DNA/RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates with anti-FLAG antibody were then incubated for additional 30 min (4°C, rotating) with 75 µl/sample pre-washed (3 times in buffer A) Protein A-Sepharose beads (Sigma-Aldrich, #P6649). Afterwards ...
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bioRxiv - Molecular Biology 2023Quote: ... lysates were clarified by centrifugation at 12000g and incubated 2h at 4°C with pre-equilibrated α-FLAG M2 affinity gel beads (Merck-Sigma, A2220). Beads were washed three times with lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... The aforementioned experiments were also repeated using 0.03 µm fluorescent green carboxylate-modified polystyrene beads (density 1.050 g/cm3, excitation 470 nm, emission 505 nm) (Sigma-Aldrich, Munich, Germany). MP bead types were characterized using FEI Quanta 600 field-emission scanning electron microscope (SEM ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were grown in 25% TSB medium at 28°C in static conditions in glass microcosms with one layer of ColiRoller glass beads (Millipore, Merck, USA) covering the base of the microcosm to provide additional spatial structure ...
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bioRxiv - Immunology 2023Quote: ... Immune mediators in cell culture supernatant following CD3/CD28 bead stimulation were measured using a human premixed CD8+ T Cell Human 17-plex panel (Millipore, Temecula, California). All Luminex assays were analyzed using a MAGPIX Instrument and xPONENT software (Luminex ...
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bioRxiv - Microbiology 2023Quote: ... the fungal pellet was suspended in 500 µL of pre-chilled DEPC-treated sterile deionized water and transferred to a 1.5 ml tube containing 200 µL of 0.5 mm glass beads (Sigma-Aldrich, St. Louis, MO). The samples were homogenized using the MillMix 20 beat-beater (Technica ...
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bioRxiv - Microbiology 2023Quote: ... Cell-laden filters were suspended in 2-ml bead beater tubes (SSIbio, # 21276) containing 1 ml of fresh metabolite extraction solvent (2:2:1 ratio of acetonitrile (Sigma-Aldrich, # 900667), methanol (>99.8%) ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was suspended in 1 mL of 1× PBS along with 500 mg of CER beads (Amberlite® IR120 Na+ form, Sigma-Aldrich), which corresponded to a ratio of 50 grams of cationic beads per gram of DW biomass ...
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bioRxiv - Biochemistry 2024Quote: ... the pellet was resuspended in 100 µL of a 10% (w/v) TCA solution and broken for 10 min with glass beads (Sigma-Aldrich, #G8772) at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... 450 µg of total protein were incubated for 20 min (rotating wheel, RT) with 50 µL of streptavidin-agarose beads (Sigma-Aldrich, #S1638). After incubation ...
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bioRxiv - Biochemistry 2024Quote: ... were incubated for 45 min at 4°C with 10 µL agarose beads using a RAS-GTP pull-down assay kit (RAS Activation Assay Kit, Merck Millipore, 17-218). Supernatant was recovered after centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... archaeal co-cultures were mixed in a 2:1 ratio with 10 nm colloidal gold beads precoated with 1% BSA (Sigma-Aldrich, Australia). The mixture was added onto glow-discharged copper R2/2 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Developmental Biology 2024Quote: Embryos collected from natural spawning were injected at the vegetal pole at 5 mpf with 1nl of 1:5 fluorescent yellow-green carboxylate-modified polystyrene latex beads (L4530, Sigma-Aldrich®) diluted in H2O ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was probed with the indicated primary antibody (rabbit anti-GFP [1:1500, Abcam, #ab290], mouse anti-RFP [1:1000, Chromotek, #6G6], and mouse anti-2A [1:1000, Sigma, #3H4]), washed with TBST ...
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bioRxiv - Cell Biology 2019Quote: ... and immunoblotted with the indicated antibodies followed by HRP-conjugated goat anti–mouse IgG or anti–rabbit IgG or rabbit anti–chicken IgY (Sigma-Aldrich) and Super Signal West Pico Chemiluminescent Substrate (PI34080 ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated overnight at 4 °C with the respective primary antibodies (rat-monoclonal anti- BrdU −1:200- Abcam; Rabbit polyclonal antibody anti-DCX,1:500- Cell Signaling; mouse monoclonal anti-NeuN- Millipore) diluted in a 0.1 % BSA and 0.125 % Triton X-100 solution ...
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bioRxiv - Cell Biology 2021Quote: ... 2013), rabbit anti-Mad2 (Heinrich et al., 2013), rabbit anti-Mad3 (Heinrich et al., 2013) or mouse anti-tubulin (Sigma, T5168). Secondary antibodies were either anti-mouse or anti-rabbit conjugated to HRP (Dianova ...
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bioRxiv - Biochemistry 2022Quote: ... mouse anti-GBP130 (1:500), mouse anti-EXP2 (1:5000, 37), rabbit anti-Nluc (1:400, 65) and mouse anti-HA (1:500, Sigma-Aldrich). After primary antibody was removed ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins transferred to nitrocellulose membranes were probed with either affinity-purified polyclonal rabbit anti-Gli326 or goat polyclonal anti-Gli3 (R&D, AF3690) and mouse anti-vinculin (Sigma, V9264) and visualized with fluorescent secondary antibodies (LI-COR IRDye 800CW ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2, ab18465, Abcam; 1:1000 Rabbit anti-GABAalpha6, G5555, Sigma-Aldrich; 1:1000 Rabbit anti-Cux1 ...
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bioRxiv - Microbiology 2020Quote: ... or anti-gL (rabbit polyclonal) antibodies and detected using anti-rabbit or anti-mouse secondary antibodies conjugated to horseradish peroxidase (Sigma-Aldrich) and Pierce ECL-Western blotting substrate (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... for 1hr and probed overnight with primary antibodies (Rabbit anti-SDMA, CST13222; mouse anti-vinculin, Sigma, RABBIT anti-PRMT5 (Abcam Ab31751). Blots were washed 3X with 1% TBST buffer and probed with secondary antibodies 680RD goat anti-Mouse and 800CW goat anti-Rabbit (LI-COR ...
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bioRxiv - Developmental Biology 2020Quote: ... Immunoprecipitation was performed using ∼2.5-5µg antibody (anti-H3K27ac: 05-1334 Millipore Sigma, anti-H3K9ac: 07-352 Millipore Sigma, anti-H3K4me3: 07-473 Millipore Sigma, anti-SATB1 ...