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Citations for Millipore Sigma :
4151 - 4200 of 10000+ citations for IL 4 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... x Talin1fl/fl (females) intercross received three consequent daily intraperitoneal injections of 4-hydroxy-tamoxifen (4-OHT, purity ≥98% Z isomer, Sigma-Aldrich Cat# H7904) (80 μg/pup diluted in ethanol/peanut oil ...
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bioRxiv - Microbiology 2024Quote: ... P3HR-1 ZHT/RHT or Akata cells lytic induction was triggered by addition of 400 nM 4-hydroxy tamoxifen (4-HT) (Sigma #68392-35-8) for 24 h or by 15 µg/ml α-human IgG (Agilent #A042301-2 ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were post fixed in 4 % PFA over night at 4°C and cryoprotected in 30 % sucrose (w/v, Sigma Aldrich, Taufkirchen, Germany). Following cryoprotection ...
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bioRxiv - Neuroscience 2024Quote: ... Dissected brains were post-fixed for 2 h at 4°C in 4% PFA and cryoprotected in successive solutions of 15% and 30% sucrose (Sigma-Aldrich, Cat# S0389) in PBS ...
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bioRxiv - Neuroscience 2024Quote: Population tagging was performed via a single intraperitoneal (i.p.) injection of either 0.9% saline or 40 mg/kg 4-hydroxytamoxifen (4-OHT; Sigma, St. Louis, MO, Cat# H627) immediately following sociability testing in the presence of predator odor on PN16-17 83–85 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples (5 μg) were used to determine GCase activity upon addition of 10 mM 4-Methylumbelliferyl ß-glucophyranoside (4-MU, Sigma-Aldrich, # M3633) in 1% BSA with or without 2 mM conduritol-b-epoxide (CBE ...
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bioRxiv - Developmental Biology 2023Quote: Vital dye staining of lateral line hair cells of the 4 dpf embryos was performed using DASPEI (2-(4-(dimethylamino) styryl)-N-ethylpyridinium iodide) (Sigma-Aldrich, USA, D0815), 0.8 µg/ml ...
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bioRxiv - Cancer Biology 2023Quote: To generate ApcKO tumoroids, Lgr5-2a-CreERT2::Apcflox/flox healthy organoids were treated with 4-hydroxytamoxifen (4-OHT, 500nM) (Sigma cat#SML1666-1ML) for 24hrs - intestinal stem cells with successful Cre-recombination activated tdTomato expression and appeared red under a fluorescence microscope ...
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bioRxiv - Pathology 2023Quote: ... 1-6 months-old adult mice were shaved on the dorsal skin and treated topically with 40 μl of 1-5mg/ml 4-hydroxytamoxifen (4-OHT; Sigma, St. Louis, MO) in ethanol every other day for 4 times ...
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bioRxiv - Cancer Biology 2020Quote: ... then incubated with primary antibodies (mouse anti-PAR, 1:400 Merck Millipore Cat#AM80 RRID: AB_2155072; mouse anti-γH2AX pS139, 1:2000 Merck Millipore Cat#05-636 RRID ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were immunoblotted with rabbit monoclonal anti-mouse caveolin-1 and rat monoclonal anti-mouse total β1-integrin (clone MB1.2, MAB1997 Millipore) as a loading control and a marker for PM fraction ...
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bioRxiv - Physiology 2023Quote: ... Mouse monoclonal anti-FLAG M2 antibody (F1804) and mouse monoclonal anti-β-actin antibody (A1978) were obtained from Sigma. HRP-conjugated goat anti-rabbit (CST#7074 ...
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bioRxiv - Immunology 2023Quote: ... the bound antibody was incubated with horseradish-peroxidase labelled subclass-specific anti-mouse IgG1 at 1:8,000 or anti-mouse IgG2c at 1:2,000 (Sigma, Aldrich). After five washes with PBS-T ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22, CRP; Millipore Sigma - RAB0096-1KT). In brief ...
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bioRxiv - Immunology 2021Quote: Degranulation and cytokine production of NK cell subsets were determined after cytokine stimulation o/n with IL-12 (10ng/ml, Sigma Aldrich, USA) and IL-18 (5ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... and carbonic anhydrase (29 kD) from a combination of two kits (GE Healthcare, catalogue No. 28-4038-41, Chicago, Illinois, IL, USA and MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Neuroscience 2021Quote: ... and phosphorylated-tau 181 (p-Tau) were measured according to manufacturer specifications (Luminex xMAP technology; EMD Millipore, Chicago, IL, USA: HNABTMAG-68K), modified by a 1:10 dilution of CSF ...
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bioRxiv - Microbiology 2020Quote: ... conjugated anti-histidine was added and color developed by addition of and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St. Louis, MO, USA). Plates were read at an OD of 450 nm with a SpectraMax Plus plate reader (Molecular Devices ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was determined using BCA™ protein assay (Pierce, Rockford, IL, USA) using BSA (Sigma- Aldrich, St. Louis, MO, USA) as a standard ...
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bioRxiv - Immunology 2022Quote: ... BLaER1 cells were trans-differentiated for 5-6 day in medium containing 10 ng/mL IL-3, 10 ng/mL M-CSF (MPI of Biochemistry, Munich) and 100 nM β-estradiol (Sigma-Aldrich) at a concentration of 7 × 104 cells/well in 96 well plates ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasma was stored at −30°C for analysis of leptin and insulin (CrystalChem Inc., Chicago, IL, USA) and triglyceride content (Roche triglyceride reagent, Sigma glycerol standard).
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bioRxiv - Microbiology 2024Quote: ... the maintenance and expansion of T cells was done by adding medium with 10 ng/ml interleukin-2 (IL-2, Stemcell) (10 ng/ml) and for activation Lectin from Phaseolus vulgaris (red kidney bean, PHA) (Sigma, 1µg/ml).
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bioRxiv - Genetics 2023Quote: Tissue lysates in RIPA buffer containing protease and phosphatase inhibitors as above were used for ELISA measurements of IL-1 beta (#RAB0275, Sigma Aldrich, GmbH) and IL-6 (#RAB0309 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were blocked with bovine serum albumin (0.5%) before labeling with anti-STIM1 monoclonal antibody (1:50 dilution; 30 min; Sigma-Aldrich, Rockford, IL). Cells were then washed before the addition of Alexa Fluor 488-conjugated goat anti-mouse IgG (1 µg/ml dilution ...
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bioRxiv - Biochemistry 2022Quote: ... The diluted lysate was incubated with antibody overnight at 4 °C with rotation: H3K4me3 (4 × 106 cells, 2.5 μg anti-H3K4me3, Millipore, Cat#07-473, Lot#3394198), H3K36me2 (10 × 106 cells ...
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bioRxiv - Immunology 2022Quote: ... At 4 hpi, macrophages and BECs were fixed with 4% paraformaldehyde (Aurion, cat. no. 15710) and immuno stained for LAMP1 (Sigma Aldrich, cat no. L1418), LPS (Invitrogen ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the rats in SCI group were received the process of SCI and administrated with normal saline and the rats in SCI+Ex-4 group were administrated intraperitoneally (i.p.) with Ex-4 (10 µg/rat) (Sigma-Aldrich, St. Louis, MO, USA) in normal saline after SCI and dosing interval was 24h for three days ...
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bioRxiv - Neuroscience 2020Quote: ... in TBST before incubating overnight at 4°C in primary antibody solution (4% NGS in TBST) with rat anti-GFAP (Millipore Cat# 345860-100UG, RRID:AB_211868), rabbit anti-GFAP (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... were used to infect subconfluent MCF7 KRT19 KO cells (KO2) in three sequential 4 h incubation in the presence of 4 µg/ml polybrene (Sigma-Aldrich, St Louis, MO). Transductants were selected in hygromycin (100 µg/ml) ...
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bioRxiv - Biochemistry 2023Quote: ... 400 ml of the culture was divided into 200 ml aliquots and 500 μl of 2M 4-thiouracil (4-sU, Sigma-Aldrich Cat# 440736-1G) was added to one of the flasks with vigorous mixing and returned to the shaking incubator for 6 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... plates were thawed at 37 ºC and incubated in darkness with 4 μg/ml Hoechst (HO33342; 2’-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5’-bi-1H-benzimidazole trihydrochloride trihydrate, Sigma-Aldrich, St Louis, MO, USA) DNA stain in solution buffer (1 M NaCl ...
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bioRxiv - Physiology 2023Quote: ... on fluid secretion rate, 10-4 M 8 bromo-cAMP (cAMP) (23, 66) and 10-8 M 8 bromo-cGMP (cGMP) (4) (Sigma-Aldrich, Oakville, ON, Canada) were used against unstimulated MTs ...
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bioRxiv - Plant Biology 2020Quote: ... 150 µM 3′,5′-Dimethoxy-4′-hydroxyacetophenone (acetosyringone; SIGMA, USA) at a 23.7×108 cfu/ml (OD600=0.75 ...
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Controlled Fluorescent Labelling of Metal Oxide Nanoparticles for Artefact-free Live Cell MicroscopybioRxiv - Biophysics 2021Quote: ... centrifugal filter device Amicon Ultra 4 mL 100K (Merck Millipore), HCl (Merck) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 million cells were fixed in 1.5% formaldehyde (Sigma F8775) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.13 g/ml Mowiol 4–88 (Sigma-Aldrich 81381-50G), 0.13M Tris.HCL (pH 8.5)).
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bioRxiv - Neuroscience 2021Quote: ... 4 Mg-ATP and 14 Tris-phosphocreatine (all from Sigma). A second glass pipette (1-3 MΩ) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then fixed with 4% fresh paraformaldehyde (Sigma, 158127) in PBS at RT for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... followed by fixation for 4 min in 1 % formaldehyde (Sigma). Cell pellets were stored at −20 °C or processed immediately.
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bioRxiv - Cell Biology 2020Quote: ... Recombination was induced with 100 nM 4-hydroxytamoxifen (4OHT, Sigma) added to the culture medium upon splitting.
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bioRxiv - Cell Biology 2020Quote: ... and protease cocktail inhibitor (Sigma;10.4 mM 4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride ...
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed in a 4% formalin solution (Sigma-Aldrich) at 4 °C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... were fixed in freshly diluted 4% formaldehyde (Sigma-Aldrich, F1635) for 15 min at RT ...
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bioRxiv - Developmental Biology 2021Quote: Tissue was harvested and fixed in fresh 4% paraformaldehyde (Sigma) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with 4 μM Hoechst 33342 (Sigma-Aldrich) as a membrane-permeable DNA stain ...
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bioRxiv - Immunology 2021Quote: Mice paws were fixed using 4% p-formaldehyde (Sigma-Aldrich) for at least 24 h ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were blocked in 4% Donkey Serum (D9663, Sigma-Aldrich) for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: Unfertilized eggs were dejellied in 4% cysteine solution (Sigma, 168149) in ASW for 9 minutes and washed with ASW ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 4′6-diamidino-2-phenylindole (1:5000, Sigma Aldrich). Images were acquired on a Leica DM5500B microscope equipped with a Leica DEC500 camera.
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bioRxiv - Neuroscience 2022Quote: ... followed by 4% paraformaldehyde (PFA; CAT# P6148-500G, Sigma Aldrich). Brains were extracted and fixed in 4% PFA at 4 °C for one week ...