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Citations for Millipore Sigma :
4101 - 4150 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: Around 750 entire-4 (e-4) seeds were treated with the mutagenic substance Ethyl-Methane Sulfonate (EMS, Sigma m0880) at a concentration of 0.6% for 10 hours ...
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bioRxiv - Biophysics 2021Quote: The ATPase activity of MutL or MutL(R-E) was measured by an ATPase/GTPase Activity Assay Kit (Sigma). The analysis was carried out with 5 μM protein in a 40 μL reaction mixture comprised of 20 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Microbiology 2022Quote: ... difficile spores were incubated with 10 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) with PBS for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were collected by filtration and promptly washed twice on the filter using one culture volume of H2O and then resuspended into YPD medium containing 0.2 mg/mL pronase E (Sigma).
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bioRxiv - Bioengineering 2022Quote: ... samples were stained with hematoxylin and eosin (H&E) through a 5- minute incubation in Harris Hematoxylin (Sigma Aldrich), followed by a wash in tap water and three dips in acid ethanol to destain ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... These sections were stained with Hematoxylin & Eosin (H&E) and periodic acid-Schiff (PAS) according to the manufacturer’s protocol (Sigma). The muscle sections were analyzed blindly by a pathologist ...
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bioRxiv - Immunology 2023Quote: Camostat (cat. no. SML0057) and E-64d (cat. no. E8640) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A short synthetic dsRNA ...
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bioRxiv - Microbiology 2024Quote: ... [cyano-(3-phenoxyphenyl)methyl] 3-(2,2-dichloroethenyl)-2,2- dimethylcyclopropane-1-carboxylate (i. e. cypermethrin, pesticide analytical standard, CAS 52315-07-8, supplier: Sigma-Aldrich, purity ≥ 90.0% ...
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bioRxiv - Bioengineering 2024Quote: ... 1L of perfusate was composed of 950 mL of William’s E hepatocyte culture media (Sigma Aldrich, Burlington, MA, USA) to supply the graft with amino acids and nutrition during perfusion ...
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bioRxiv - Neuroscience 2023Quote: ... The final concentrations of drugs used in the study: 10 μM of E-64 (Sigma-Aldrich, Cat.: #E3132-1MG), 60 nM of t-NED19 (Bio-techne ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were washed three times with buffer E and then mixed with 60 μg of 3xFLAG peptide (SIGMA) in 60 μl of buffer E for 1 h with shaking at 1400 rpm at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... The target protein was eluted by application of 5 CVs of buffer E containing 2.5mM d -desthiobiotin (Sigma-Aldrich). Elutions were pooled ...
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bioRxiv - Developmental Biology 2024Quote: ... for 1 min 30 seconds at high intensity in a 200 mTorr vacuum and then incubated at room temperature for 1h with 200µL/well of 200 µg/mL Poly-D-Lysine (A-003-E, Merck Millipore) diluted in 0.1M HEPES pH 8.4 (H3784-25G ...
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bioRxiv - Cell Biology 2019Quote: ... GST (clone 8-326, MA4-004 from Thermo Fischer, 1:1000) and Cyclin B (C8831 from Sigma, 1:1000). The secondary antibodies used for Western blotting were goat ⍰ -mouse IgG HRP conjugate (170-6516 from Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... sections were stained with primary (1:1000, guinea pig anti-NeuN, Millipore #ABN90P; 1:1000, mouse anti-NeuN, Millipore #MAB377 ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated with Anti-dCaMKII Clone 18 (1:1000, CosmoBio) and anti-actin mAb C4 (1:1000, Millipore). The secondary antibody used was ...
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bioRxiv - Cell Biology 2020Quote: ... The lysates were centrifuged at 14,000 rpm for 15 minutes and supernatant mixed with T7 Tag Antibody Agarose (EMD Millipore) for immunoprecipitation or NeutrAvidin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... DNA encoding the POTRA domains (1-4) of BamA with a C-terminal strep-tag (but without the signal sequence) was cloned in pET21a (Novagen). All the proteins above were overexpressed in BL21(DE3 ...
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bioRxiv - Microbiology 2021Quote: ... encoding PPK with a C-terminal GAAEPEA peptide tag for affinity purification (105) between the NdeI and HindIII sites of plasmid pET-21b(+)(Novagen), was synthesized by GenScript.
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bioRxiv - Immunology 2019Quote: The sequence-encoding residues Val77–Leu233 of human TNFα was cloned and fused to the N-terminal 6xHis tag in pET-28a+ vector (Novagen) and transformed into Escherichia coli Rosetta (DE3 ...
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bioRxiv - Molecular Biology 2020Quote: ... was replaced by the wild-type CARD14 gene obtained from pFN21AE3344 with N-terminal 3xFLAG tag peptides (DYKDHDGDYKDHDIDYKDDDDK) (Sigma-Aldrich). To generate CARD14-expressing vector (pTRE3G-Pur-3FLAG-CARD14wt ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-ZO1 (D7D12, CSL), anti-ZO2 (2847, CSL) or Alexa Fluor 488 conjugated anti-DYKDDDDK Tag monoclonal antibody (L5, Sigma) in 5 % BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... was PCR amplified from yeast constructs described above and inserted into the Nde1 and BamH1 sites downstream of sequence encoding the His6 tag and the thrombin cleavage sequence in bacterial expression vector pET28a (Novagen). IDT g-blocks® fragments were synthesized for wild-type UBE2A or UBE2B or their mutants and inserted into Nde1-BamH1-digested pET28a by sequence and ligation independent cloning (SLIC ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was used for FLAG-tag affinity purification with 1.2 µL of a 50% slurry of FLAG-M2 beads (Sigma-Aldrich) per 1 mL of culture ...
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bioRxiv - Cell Biology 2019Quote: ... cells were permeabilized with 0.2% Triton-X100 or 0.1% Saponin and stained with antibodies directed against the FLAG tag (M2, Sigma, 1:400), Golgin97 (CDF4 ...
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bioRxiv - Molecular Biology 2019Quote: ... The activated beads were resuspended in 100 μL 50 mM MES (pH 5.0) and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804 ...
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA sequence corresponding to sim-PD (aa 361-672) was PCR amplified and cloned in-frame with a 6xHis-Tag into a modified pET-14b plasmid (Novagen). The expression plasmid was transformed into BL21 competent E ...
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bioRxiv - Biophysics 2019Quote: ... Tag cleavage was carried out overnight at 4 °C on a roller with 10 units of HRV 3C protease (Novagen/Merck chemicals Boulevard Industrial Park ...
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bioRxiv - Cancer Biology 2019Quote: HeLa was transfected with Flag-tagged Vav1 and V5-tagged VEGFR1 grown on coverslips were stained with antibody against Flag-tag (Sigma), and against V5-tag (Cell Signaling) ...
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bioRxiv - Microbiology 2021Quote: ... The hSPLUNC1 coding sequence with a 3’ sequence encoding the FLAG tag was amplified from cDNA using KOD polymerase (Sigma) and the primers (Forward ...
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bioRxiv - Biochemistry 2020Quote: ... was fused to an N-terminal TEV-cleavable His6-GCN4 tag (as described in Sosa et al. (2012)) and cloned into a pRSF-Duet1 (Merck Millipore) vector ...
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bioRxiv - Biochemistry 2021Quote: ... Purifications using 3xFlag tag were performed similarly with the following modifications: 1 ml slurry of ANTI-FLAG M2 Affinity gel (Sigma) equilibrated in flag lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... the Phos-tag gels were incubated 4 × 10 min in 100 mM EDTA before electrophoretic transfer onto Nitrocellulose membrane (Sigma). Proteins were detected by immunoblotting using the following primary antibody concentrations in 5% milk (VWR)/Tris Buffered Saline -Tween20 (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... was cloned using cDNA from Arabidopsis seedlings and inserted in frame with an N-terminal His6-tag between the BamHI and EcoRI sites of the pETDuet-1 vector (Novagen). Protein expression was performed in E ...
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bioRxiv - Microbiology 2020Quote: ... All purified proteins were analyzed by SDS-PAGE followed by Coomassie blue staining as well as by Western blot using a mouse monoclonal antibody against the 6xHis-Tag (Novagen). Protein aliquots were stored at −80⁰C in 50% glycerol (v/v ...
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bioRxiv - Evolutionary Biology 2020Quote: ... brucei ALPH1 was expressed as an N-terminal truncation with a C-terminal 6xHis-Tag in RosettaBlue competent bacteria cells (Novagen) as previously described [10].
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bioRxiv - Microbiology 2021Quote: ... membranes were blocked for 1 h with 5% dried skim milk in 1X PBS containing 0.1% Tween 20 (T-PBS) and incubated with primary anti-HA epitope tag (Sigma, H6908) or anti-GFP (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mg/L avidin and 6 mM MgCl2 were added to the supernatant to improve the Strep tag binding efficiency with Strep-Tactin beads (Novagen). Next ...
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bioRxiv - Plant Biology 2021Quote: ... This was achieved using Western blotting detecting the HA-tag (antibody: Anti-HA-Peroxidase, Sigma-Aldrich, St. Louis, USA, 12013819001) and resistance against hygromycin-induced discoloration ...
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bioRxiv - Systems Biology 2020Quote: ... the cDNA was inserted in frame with a 6xHis-GST TEV-cleavable tag into a modified version of pET-30b (Novagen). The NCK2 SH3 shuffled chimeras were constructed using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... The analyzed proteins contained an SHH-tag (Sarkari et al., 2014) and were detected using anti-HA (Sigma-Aldrich, USA) antibodies and anti-mouse IgG-HRP (Promega ...
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bioRxiv - Microbiology 2020Quote: Monoclonal antibody against FLAG tag (ANTI-FLAG M2) and β-Actin were purchased from Sigma (Cat.No. F1804 and A2228, respectively). Monoclonal antibody against human IFITM1 (Cat.No ...
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bioRxiv - Biophysics 2021Quote: ... The GB1 tag was cleaved from the oxidized proteins by incubating overnight with thrombin (10 U.mg−1 protein, Sigma-Aldrich). The cleaved protein was purified by reversed phase high-performance liquid chromatography (RP-HPLC ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was resuspended in digest buffer (0.1 M triethylammonium bicarbonate, pH 8.5, Sigma-Aldrich, tandem mass tag (TMT) labeling using a 6-plex TMT kit (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... in this work referred to as Pif1) fused at its N-terminus to a 6-histidine tag was cloned into vector pET28 (Novagen) and transformed into E ...
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bioRxiv - Microbiology 2022Quote: ... and incubated overnight at 4°C with specific primary monoclonal (MAbs) or polyclonal (pAbs) antibodies: anti-HA tag (rabbit pAb; H6908, Sigma), anti-GFP (rabbit pAb ...
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bioRxiv - Biochemistry 2020Quote: ... and a His8 tag was ligated into the corresponding sites in the first multiple cloning site of pCDFDuet-1 (Novagen), yielding plasmid pRHJ001 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 5% skimmed milk in Tris Buffered saline-Tween for 1 h before incubation with primary antibody anti-myc tag monoclonal clone 4A6 (Millipore) diluted to final concentration of 200 ng mL−1 in 3% skimmed milk in TBST for 1h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Removal of the GST-tag from human and zebrafish Vps29 was performed using on-column cleavage with thrombin (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: Recombinant 6XHis-tag GreA or 6XHis-tag GreB were produced by cloning greA and greB genes into NdeI and BamHI sites of the pET14B vector (Novagen) using greA F and greA R or greB F and greB R primers ...