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Citations for Millipore Sigma :
4101 - 4150 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Darmstadt, Germany) using a BioRad transfer machine after being run through 10% SDS-polyacrylamide (SDS-PAGE ...
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bioRxiv - Cancer Biology 2021Quote: ... Resolved protein was transferred to PVDF membranes (Millipore, Billerica, MA, USA) using a Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were detected using either Luminata Crescendo Western HRP Substrate (Millipore) or ECL Western Blotting Analysis System (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then transferred to an Immobilon-P transfer membrane (Millipore) using a Trans-Blot Turbo Transfer system (BIO-RAD) ...
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bioRxiv - Cell Biology 2019Quote: ... and included protein phosphatase inhibitor cocktails I and III (Sigma-Aldrich) in cell extracts ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration in the supernatant was measured using Bradford Reagent (Sigma), and equal amounts of protein were resolved by SDS-PAGE.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were then transferred to PVDF membranes (Millipore, Burlington, MA, USA) and blotted with anti-phospho-Akt (Ser473 ...
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bioRxiv - Genetics 2021Quote: ... The expressed proteins were partially purified on Q sepharose (Sigma q1126) and the fractions containing the protein of interest were collected ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to 0.45 μm Immobilon-FL PVDF membranes (Millipore), and membranes were blocked in either 5 % BSA or Odyssey Blocking buffer (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... Separated proteins were transferred to PVDF-FL transfer membrane (Merck Millipore) at 40V for 90 min at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were reduced with 1.5 µmol of DTT (Sigma-Aldrich, Canada) for 20 minutes at 70 degrees Celsius ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells where then lysed with BugBuster protein extraction reagent (Novagen) for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-surfactant associated protein C (SFTPC, 1:1000, Millipore, AB3786), goat anti-podoplanin (PDPN ...
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bioRxiv - Immunology 2020Quote: A commercial protein-A affinity chromatography called PURE-1A (Sigma-Aldrich.) was used to purify anti-anti-SpA antibodies (Ab-3 ...
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bioRxiv - Developmental Biology 2020Quote: ... and protein concentration was determined using the Direct Detect spectrometer (Millipore). 20 µg of protein per sample was denatured at 70°C for 10 mins in 1× SDS Loading Dye (100 mM Tris pH 6.8 ...
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bioRxiv - Developmental Biology 2020Quote: ... the proteins were transferred onto a PVDF membrane (EMD Millipore, IPVH00010). The membranes were blocked with StartingBlockTM T20 (TBS ...
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bioRxiv - Immunology 2022Quote: Protein lysate was extracted using CelLytic B Cell Lysis Reagent (Sigma) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... protein bands were visualized using an enhanced chemiluminescence detection kit (Millipore) and and the Syngene Pxi4 imaging system (Ozyme) ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred by wet transfer to a PVDF membrane (Millipore) at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... AID-tagged proteins were conditionally depleted using 250mM auxin (Sigma, I2886) stock dissolved in ethanol at a final concentration of 0.5mM ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 µl of pre-washed protein A beads per sample (Sigma, equilibrated to lysis buffer as mentioned in the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred to Immobilon FL (EMD Millipore Corp IPFL00010) membrane in transfer buffer containing 10% methanol and 0.2% SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected with primary antibodies to FLAG M2 (Sigma, A8592), HA 3F10 (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... and house-keeping protein GAPDH (1:500; Mouse; Sigma-Aldrich G8795) or β-actin (1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... was used to transfer proteins to PVDF Immobilon membranes (Merck Millipore). Blocking and antibody incubation steps were performed in EveryBlot Blocking Buffer (BioRad) ...
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bioRxiv - Genomics 2020Quote: ... Eluted protein was concentrated by centrifugal ultrafiltration (Millipore Amicon Ultra, 10K), and loaded onto a pre-equilibrated HiLoad Superdex 75 16/60 column (GE Healthcare ...
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bioRxiv - Systems Biology 2019Quote: ... The 1 mg/mL pure bovine glutamate dehydrogenase (GDH) protein (Sigma) was reacted with 1 mM DSSO in 50 mM HEPES buffer ...
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bioRxiv - Genetics 2019Quote: ... Proteins were detected with an enhanced chemi-luminescence system (Millipore Luminata) and visualized using an Amersham Imager 600 (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred on Immobilon-FL membrane (Millipore, prod. number IPFL00010), blocked for 1 hr with blocking buffer (LiCOR prod ...
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bioRxiv - Epidemiology 2019Quote: ... The β subunit protein from cholera toxin was purchased from Sigma Chemical ...
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bioRxiv - Biochemistry 2020Quote: ... Digested glycans were cleaned using a PVDF protein-binding membrane (Millipore) and analyzed as described above.
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bioRxiv - Molecular Biology 2019Quote: ... After dialysis protein was concentrated using 10kDa MWCO Amicon filter (Millipore). In vitro SUMOylation assay was performed using SUMO2 Conjugation Kit (BostonBiochem ...
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bioRxiv - Immunology 2019Quote: ... Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore) visualized on Hyperfilm™ (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... protein spots were stained with Direct Blue (Sigma-Aldrich, MO, USA). Membrane spots were excised and washed in separate wells in a flat bottom polypropylene 96-well plate ...
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bioRxiv - Molecular Biology 2019Quote: ... and the proteins were transferred onto a PVDF Transfer Membrane (Millipore). The membrane was incubated in blocking buffer (5% nonfat milk in Tris-buffered saline with 0.05% Tween 20 ...
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bioRxiv - Biophysics 2019Quote: ... then incubated with 0.5μg/ml biotin-coupled Protein-A (Sigma-Aldrich) for 1 hour under stirring at room temperature and rinsed with 0.1% BSA ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were transferred onto Immobilon-FL PVDF membranes (Millipore; Cat # IPFL00010), and nonspecific protein binding was blocked with 3% BSA in Tris-buffered saline for at least one hour at room temperature ...
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bioRxiv - Physiology 2020Quote: ... Tissue proteins were isolated by homogenization in RIPA buffer (Sigma-Aldrich) supplemented with cOmplete Mini protease inhibitor cocktail tablets (Roche Diagnostics ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were concentrated using Amicon Ultra filters (Millipore, Ireland), and confirmed by SDS-PAGE.
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bioRxiv - Biochemistry 2019Quote: ... proteins were transferred to 0.45 µm Immobilon-P PVDF membranes (Millipore) pre activated in methanol ...
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bioRxiv - Plant Biology 2020Quote: ... Transferred proteins were probed with anti-tubulin (Sigma, 1:10,000 dilution) and anti-GFP (Takara Bio ...
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bioRxiv - Neuroscience 2019Quote: ... protease inhibitors and protein phosphatase inhibitors I and II (Sigma-Aldrich). After an incubation of 30 min on ice and centrifugation at 10 000g for 10 min ...
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bioRxiv - Plant Biology 2020Quote: ... All proteins were expressed in BL21 Rosetta DE3 pLysS cells (Novagen) and purified using IMAC as described in detail previously41.
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bioRxiv - Cell Biology 2019Quote: ... proteins transferred to PVDF membranes (Immobilon-FL or Immobilon-P, Millipore) were detected by fluorophore- or HRP-conjugated to secondary antibodies (Cy5 AffiniPure Goat Anti-Rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2019Quote: ... and 80 ug/mL myelin basic protein (MBP, Sigma-Aldrich #M1891) in the presence of 25 uM ATP (Sigma-Aldrich A7699) ...
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bioRxiv - Cell Biology 2019Quote: Proteins/peptides were labelled with atto-488 NHS ester (Sigma, 41698). Free dye was separated from labelled protein using G25 desalting columns ...
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bioRxiv - Systems Biology 2019Quote: ... Secondary incubation with anti-Protein G HRP conjugated (Millipore 18-161) at 1:10,000 was performed at RT during one hour ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The total protein extracted was quantitated using Bradford Reagent (Sigma, USA). Bovine Serum Albumin (BSA ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were electro-transferred to PVDF membranes (Merck Millipore, MA, USA). Immuno-detection was performed with polyclonal antibodies ...
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bioRxiv - Bioengineering 2021Quote: ... mouse anti-glial fibrillary acidic protein (GFAP)–Cy3 (Sigma, 1:2000) to show astrocyte structure ...