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Citations for Millipore Sigma :
4101 - 4150 of 10000+ citations for 6 Ethyl 1H indole 2 3 dione 3 O 4 4 4 trifluoro butyl oxime since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... 107 cells cultured with 100nM 4-OHT or ethanol control for 6 or 24 hours were incubated with 1% formaldehyde (Sigma-Aldrich, F8775) at room temperature for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... Y1eGFP mice at 6-8 weeks of age were transcardially perfused with chilled phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (Sigma-Aldrich, USA) in PBS ...
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bioRxiv - Physiology 2023Quote: ... mice were fasted for 6 hours followed by oral gavage of 150 µL of 80 mg/mL FITC-dextran (Sigma, 4 kDa). Blood was collected via tail vein at baseline and 1-hour post-gavage ...
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bioRxiv - Systems Biology 2023Quote: ... K562 cells were transduced in 6-well plates by mixing the cells and virus with 1:500 dilution of 4 mg/mL Polybrene (Sigma H9268-5G). For spinfection ...
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bioRxiv - Cell Biology 2023Quote: ... permeabilized for 4-6 h with 10% goat serum in PBS containing 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA), and incubated overnight at 4°C in primary antibody in PBS containing 0.1% Tween 20 (PBS-Tw ...
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bioRxiv - Neuroscience 2023Quote: ... then in the anti-dopamine receptor antibody diluted in the blocking solution for 5-6 days at 4°C (rabbit anti-dopamine D1A receptor, 1:100, Sigma-Aldrich AB1765P). Sections were washed in PBS and incubated for 2 h at RT in the secondary antibody (goat anti-rabbit Alexa Fluor 555) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were permeabilised overnight at 4°C and then transferred to blocking solution (10% DMSO, 6% normal donkey serum (NDS; Sigma-Aldrich, D9663), 0.2% Triton X-100 in DPBS ...
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bioRxiv - Neuroscience 2019Quote: ... and kinate receptors was achieved with 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione (NBQX; 50 µM; Sigma, Australia), N-methyl-D-aspartate (NMDA ...
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bioRxiv - Cell Biology 2021Quote: ... 3 (Sigma) in de-ionised (DI ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... 3 (Sigma) as counterstain.
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bioRxiv - Molecular Biology 2023Quote: ... were cleaned by sonication with 3 M NaOH then Piranha solution (2:3 ratio of 30% (w/w) H2O2 to sulfuric acid) and treated with 3-glycidyloxypropyl trimethoxysilane (GOPTS) (Sigma). GOPTS was then reacted with a mixture of 9 parts α-hydroxy-ω-amino PEG 3000 to 1 part α-biotinyl-ω-amino PEG 3000 by weight (Rapp Polymere) ...
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bioRxiv - Molecular Biology 2022Quote: ... The FahPM/PM mice were kept on water supplemented with 10 mg/L 2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione (NTBC; Sigma-Aldrich, Cat. #: PHR1731-1G). Mice with more than 20% weight loss were humanely euthanized according to IACUC guidelines ...
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bioRxiv - Cell Biology 2022Quote: ... pLKO.1-puro-CMV-tGFP plasmids targeting SNX5 (5’-ACTATTACAATAGGATCAAAG-3’, 5’-CTGAGTATCTCGCTGTGTTTA-3’) and SNX6 (5’-AGTAAAGGATGTAGATGATTT-3’, 5’-GCCGAAACTTCCCAACAATTA-3’) (Sigma-Aldrich) were lentivirally transduced ...
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bioRxiv - Cancer Biology 2021Quote: ... MYC forward 5’-TGAGGAGGAACAACAAGATG-3’ and reverse 5’-ATCCAGACTCTGACCTTTTG-3’ and GAPDH forward 5’-CTTTTGCGTCGCCAG-3’ and reverse 5’-TTGATGGCAACAATATCCA-3’) (Sigma-Aldrich), the Radian SYBR Green Lo-ROX qPCR Kit (Alkali Scientific ...
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bioRxiv - Immunology 2022Quote: ... The sequences of forward and reverse primers for εGLT (fw: 5′-CTGTCCAGGAACCCGACAGA-3′; rev: 5′-TGCA GCAGCGGGTCAAG-3′) and γ1GLT (fw: 5’-CCAGGGCAGGGTCAGCA-3’; rev: 5’-GGTGCTCTTGGAGGAGGGT-3’) (Sigma-Aldrich) and their corresponding FAM-labelled MGB probes (εGLT ...
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bioRxiv - Developmental Biology 2021Quote: Live zebrafish embryos and larvae were first anesthetized by bath application of 0.02% w/v solution of 1025 Ethyl-3-aminobenzoate methanesulfonate (Sigma-Aldrich, St. Louis) in filtered fish system water for 1 min ...
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bioRxiv - Neuroscience 2021Quote: ... Individuals were euthanized with a 0.1% solution of ethyl 3-aminobenzoate methane sulfonate (MS-222; Sigma, St. Louis, MO, USA; A-5040). The brains were removed and fixed by immersion in 4% w/v paraformaldehyde in 0.1 M phosphate buffer (PB ...
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bioRxiv - Pathology 2020Quote: Enzyme assays and x-gal staining require the use of the following materials: 4-methylumbelliferone (4-MU) (Sigma Aldrich, Cat. No. M1381, St. Louis, MO), 10N NaOH (BDH ...
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bioRxiv - Developmental Biology 2021Quote: ... CreERT2 activity was induced at 75% epiboly with E3 containing 10 μM final concentration of (Z)-4-Hydroxytamoxifen (Sigma Aldrich, H7904, abbreviated as 4-OHT). 4-OHT stock was dissolved in DMSO as 10 mM single-use aliquots stored at −20°C in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1 mL per 10 g of body weight) and were perfused intracardially with 0.9% NaCl followed by 4% paraformaldehyde (PFA) (Sigma-Aldrich, #P6148; CAS: 30525-89-4). Brains were collected and were kept overnight in 4% PFA ...
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bioRxiv - Biochemistry 2022Quote: ... MK-4 or DHNA + MK-4 (prepared from the following stock solutions: 100 mM DHNA dissolved in DMSO and 50 mM MK-4 [Sigma-Aldrich] dissolved in 100% ethanol). Plate wells contained a final concentration of 106 CFU/well with 0 ...
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bioRxiv - Biophysics 2023Quote: ... TEMPO-labeling was performed with a 10-fold molar excess of 4-maleimido-TEMPO (4-Maleimido-2,2,6,6-tetramethyl-1-piperidinyloxy; Sigma-Aldrich; 200 mM stock in 100% d6-DMSO) and the peptide was incubated ON at room temperature in the dark ...
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bioRxiv - Developmental Biology 2023Quote: Dorsal pancreatic buds were microdissected from Krt19-CreERT;R26-mTmG embryos at E12.5 and cultured for 48h in BME supplemented with 4-Hydroxytamoxifen (4-OHT) (Sigma H6278, final concentration 1 μg/ml). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... except the saline contained an additional 1 mM of 4-aminopyridine (4-AP, a voltage- gated K+ channel blocker, Sigma-Aldrich, St Louis, MO, USA) or 1 mM tetraethylammonium chloride (TEA ...
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bioRxiv - Biochemistry 2019Quote: ... The plate was developed with 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (Sigma), and analysed by spectrophotometry at 405 nm ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μL of azino-bis-3-ethylbenthiazoline-6-sulfonic acid (ABTS liquid substrate; Sigma) was added and incubated in the dark at room temperature for 20 minutes and 50 μL of 1% SDS was used to stop the reaction ...
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bioRxiv - Biochemistry 2020Quote: ... TsFpn was then concentrated to 3-6 mg/ml (Amicon 50 kDa cutoff, Millipore) and loaded onto a size-exclusion column (SRT-3C SEC-300 ...
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bioRxiv - Microbiology 2020Quote: ... 100 μL of azino-bis-3-ethylbenthiazoline-6-sulfonic acid (ABTS liquid substrate; Sigma) was added and incubated in the dark at room temperature and 100 μL of 1% SDS was used to stop the reaction ...
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bioRxiv - Microbiology 2020Quote: ... 50 μL of azino-bis-3-ethylbenthiazoline-6-sulfonic acid (ABTS liquid substrate; Sigma) was added and incubated in the dark at RT and 50 μL of 1% SDS was used to stop the reaction ...
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bioRxiv - Neuroscience 2021Quote: ... and 6-chloro-1,2-benzisoxazol-3(2H)-one (CBIO) were purchased from Sigma Aldrich. Phorbol 12-myristate 13-acetate was purchased from Cayman Chemical ...
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bioRxiv - Immunology 2024Quote: ... 6 uM pargyline and 50 uM (-)-epigallocatechin-3-gallate (EGCG) (all from Sigma Aldrich). For metabolic pathway analysis ...
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bioRxiv - Neuroscience 2023Quote: ... 3 mM Na+pyruvate (Sigma-Aldrich; St. Louis, MO; CAS No. 113-24-6), 2 mM N-acetyl-L-cysteine (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... cultured neurons from floxed conditional AP-2 KO mice expressing a tamoxifen-inducible Cre recombinase were treated with 0.25 μM (Z)-4-hydroxytamoxifen (Sigma) at DIV3 ...
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bioRxiv - Molecular Biology 2020Quote: ... then 10min at 4°C in the Lysis buffer 2 (50mM Tris pH8, 10mM EDTA, 0.5% NP-40, Complete protease inhibitor (Sigma)) and subsequently sonicated in 15ml conical tubes with a Bioruptor Pico (Diagenode ...
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bioRxiv - Immunology 2022Quote: ... for 2 or 4 days in complete culture medium composed of RPMI supplemented with 10% foetal calf serum (Sigma), 0.05mM 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2019Quote: ... and 10 μM biotin-tubulin were incubated in BRB80 (80 mM PIPES pH 6.85, 2 mM MgCl2, 1 mM EGTA) with 4 mM GTP (Sigma) for 25 min at 37°C in a total volume of 25 ul ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were cleared by centrifugation and incubated at 4°C for 1.5–2 h with anti-FLAG M2 affinity gel (Sigma). After extensive washing with native IP buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary acinar cells were pelleted (80 g, 2 min, at 4 °C) and re-suspended in Waymouth media (Sigma). To obtain acinar-cell-conditioned media ...
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bioRxiv - Neuroscience 2020Quote: (T-4)-[(1E,6E)-1,7-Bis[4-(dimethylamino)phenyl]-1,6-heptadiene-3’5-dionato-kO3’kO5] difluoroboron (CRANAD-2) [26] was purchased from Sigma-Aldrich AG ...
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bioRxiv - Microbiology 2021Quote: ... and the reaction was started with 100 μl of 4 mg/ml 2-nitrophenyl β-galactopyranoside (ONPG, Sigma-Aldritch) in Z buffer and stopped with 250 μl of 1M Na2CO3 ...
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bioRxiv - Neuroscience 2020Quote: ... 2-4 day old adult males raised in normal cornmeal food were transferred to food containing RU486 (mifepristone, Sigma) in 80% ethanol to a final concentration 200 μg/ml or with the same amount of ethanol (vehicle ...
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bioRxiv - Cell Biology 2020Quote: ... on ice and in a cold room at 4°C for 2 hours then warmed up to 37°C in complete medium with 10μM Nocodazole (M1404, Sigma) and incubated until fixation ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: Mice aged 6 months or naked mole-rats aged 2-4 years were given intraperitoneal (i.p.) injections with 150mg/kg 5-Fluorouracil (5-FU; Sigma) from a 50mg/ml stock in DMSO diluted with sterile 0.9% sodium chloride solution (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 xg at 4°C for 2 min and Pah1-FLAG was immunoprecipitated with anti-FLAG agarose beads (Sigma) at 4°C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were incubated for 2 h on a rotating wheel at 4°C with anti-FLAG M2 beads (Sigma). Beads were washed once with lysis buffer (without benzonase ...
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bioRxiv - Cell Biology 2019Quote: ... #4 inguinal mammary glands were stained for several hours in carmine-alumn staining solution (2% w/v Carmine, Sigma; 5 % w/v Aluminium Potassium Sulfate ...
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bioRxiv - Neuroscience 2021Quote: Rat and mouse primary cortical neurons and BE(2)-M17 cells were fixed using 4% paraformaldehyde (PFA, Sigma-Aldrich) in PBS pH 7.4 for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 2 days at 4°C in goat anti-calretinin (1:200; Cat#AB1550, EMD Millipore), followed by 2.5 hours incubation (RT ...
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bioRxiv - Cell Biology 2019Quote: ... were fully digested for 2 days at 4 °C in 0.1M of HCl digestion solution containing 1 mg/ml pepsin (Sigma). Collagen content was measured using the Sircol collagen assay kit (Biocolor) ...
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bioRxiv - Zoology 2020Quote: ... We used standard dilutions of hen egg white lysozyme (0.5, 0.8, 1, 2, 4, 8, 10, 20, and 40 µg/ml; Sigma-Aldrich) to prepare a standard curve in each plate ...