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Citations for Millipore Sigma :
4051 - 4100 of 10000+ citations for Dectin 1 CLEC7A Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The chromatic mutants were mixed in different ratios (1:1, 1:3, 3:1) and the mixtures were fixed using 4% paraformaldehyde (Sigma−Aldrich/Merck, Germany) for 4 h at 4°C.
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bioRxiv - Cell Biology 2021Quote: βII-spectrin-KO CRISPR cell lines were generated as follows: a predesigned vector containing a human βII-spectrin-targeting gRNA and eCas9-2A-tGFP was purchased from Millipore Sigma (MISSION gRNA ID: HSP00000399630). 24 hours after transfection of this plasmid ...
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bioRxiv - Cell Biology 2021Quote: Human peripheral blood mononuclear cells (PBMC) were isolated from whole blood by Ficoll density gradient using Ficoll Plaque Premium (Sigma GE Healthcare, #17-544-02) or BD Vacutainer CPT Tubes (BD ...
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bioRxiv - Neuroscience 2021Quote: ... hiPSC lines were dissociated to form embryoid bodies and cultured on mitomycin C-treated SNL murine fibroblast feeder cells in standard human embryonic stem cell culture (hESC) medium (DMEM/F12; Sigma Aldrich, St. Louis, MO, USA) containing 20% knockout serum replacement (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... codon optimized for expression in human cell lines and modified to include the human ER localization signal was cloned into the expression vector (Catalog Nr: OGS606-5U) under the EF1α promoter (Sigma Aldrich, St. Louis, MO, USA). Positive clones were verified by Sanger sequencing (Microsynth ...
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bioRxiv - Cancer Biology 2020Quote: ... On this plasmid a single primer PCR with primer 5’-GCA AGC CCT GAA AGC GCA AG-3’ and the cDNA human HIF-1α in a p3XFLAG-CMV™-10 expression vector (Sigma, St Louis, MO, USA) (Gort et al. ...
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bioRxiv - Microbiology 2022Quote: ... and grown as undifferentiated 3D cultures in 500 µL IntestiCult™ Organoid Growth Medium (OGM) Human (STEMCELL™ Technologies, cat# 06010) supplemented with 10 µM Y-27632 (Sigma-Aldrich, cat# Y0503). Undifferentiated monolayers were produced by plating single cell suspensions obtained from 7 days old highly dense 3D cultures ...
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bioRxiv - Developmental Biology 2023Quote: ... following intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (∼ 90 hours pre-collect) and 5 IU human chorionic gonadotropin (hCG; Sigma; ∼40-48 hours pre-collect) and mating with BDF1 male mice (Jackson 100006) ...
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bioRxiv - Developmental Biology 2023Quote: ... COCs were placed in a 100-μl droplet of human tubal fluid (HTF) medium supplemented with 4 mg/ml BSA (Sigma-Aldrich, St. Louis, MO, USA)28 ...
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bioRxiv - Pathology 2023Quote: ... was performed in a representative subset of our participants using an adapted version of the MILLIPLEX® Human High Sensitivity T Cell Magnetic Bead Panel (Millipore Sigma, Burlington, MA, USA). The MAGPIX® Luminex analyzer (Luminex Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human mesenchymal stem cells (MSCs) were cultured on gelatin coated flasks for 2-3 days in Alpha-MEM (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 1% pen-strep (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... After washing, IgE antibodies were detected using biotinylated goat anti-human IgE (Vector, Burlingame, CA, USA) and streptavidin peroxidase (Sigma-Aldrich, St. Louis, MO, USA). The wells were then washed again with PBST buffer and incubated with 100 ul 3,3’,5,5’- Tetramethylbenzidine (Kirkegaard and Perry Laboratories ...
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bioRxiv - Immunology 2024Quote: ... and IFN-γ were measured in the full Generation R cohort using the Human High Sensitivity T-Helper Cells Custom 5-plex assay from Millipore (Millipore, St. Charles, MO, USA) on the Luminex™ 100 system (Luminex ...
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bioRxiv - Microbiology 2024Quote: ... blood mononuclear cells were isolated by density gradient centrifugation from buffy coats of human healthy donors (Ficoll-Paque Premium, Sigma Aldrich, St. Louis, MI, USA). Monocytes were purified from peripheral blood mononuclear cells by positive selection with magnetic CD14 MicroBeads (Miltenyi Biotech ...
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bioRxiv - Cancer Biology 2024Quote: ... Myc (CST 71D10, 1:1000), p-EGFR Y1068 (CST 3777S, 1:1000), EGFR (CST 4267, 1:1000) and β-actin (Sigma A1978, 1:10000). Densitometry analysis was performed with ImageJ software (NIH).
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin (B-5-1-2) (1:10000; Sigma-Aldrich T5168, B-5-1-2), mouse anti-GAPDH (1:25000 ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM EDTA, 1% NP40, 0.1% SDS, 0.5% Deoxycholate, 10 mM DTT, 0.5% PVPP, 1% protease Sigma inhibitor and 1 tablet of Roche protease inhibitor for 50 ml of buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Human ITGB1 (encoding integrin β1) primer #1 (H_ITGB1_2) and ITGB3 (encoding integrin β3) primer #1 (H_ITGB3_1) were purchased from Sigma-Aldrich.
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bioRxiv - Molecular Biology 2022Quote: ... Incubation with primary antibodies (anti-V5 at 1:1,000; ThermoScientific, anti-HA at 1:1,000; ThermoScientific, anti-tubulin at 1:5,000; Sigma) was performed at 4 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... rifampicin (50 μg mL-1) or surfactin (1 μg μL-1) (Sigma-Aldrich, St. Louis, MO, USA) was added.
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bioRxiv - Neuroscience 2019Quote: ... Antibodies Bach1 (1:500) or Bach2 (1:2000) were co-incubated with TH (1:10,000, Sigma, T1299). After primary incubation and washes in Tris ...
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bioRxiv - Immunology 2021Quote: ... Positive control wells containing PMA (50 ng mL−1) and Ionomycin (1 μg mL−1) (Sigma-Aldrich) were seeded with a reduced number of PBMCs ...
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bioRxiv - Microbiology 2020Quote: ... For the phage precipitation, DNase (2.5 U ml-1, Thermo) and RNase (1 μg ml-1, Sigma) were added to the filtered lysate before incubation at 37°C for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... Immunoblotting was performed using an anti-Nolz-1 antibody (1:1000) and anti-actin (1:10000, Sigma). Secondary antibodies were HRP-conjugated goat anti-rabbit IgG and goat anti-mouse IgG (Jackson ImmunoResearch Laboratories) ...
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bioRxiv - Molecular Biology 2024Quote: ... GFP-1020, 1:5,000; FLAG, Sigma, F1804, 1:1,000; FLAG, Rockland, 600-401-383, 1:1,000; GAPDH, Sigma, G9545 ...
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bioRxiv - Immunology 2023Quote: ... 0.1 % Tween-20, 0.1 % IGEPAL CA630, 0.01 % digitonin, 1 % BSA, 1 mM DTT, 1 U/µL Sigma Protector RNase inhibitor in nuclease free water ...
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bioRxiv - Microbiology 2023Quote: ... Filtered virus-containing medium was mixed 1:1 with fresh HaCaT media and 1:1000 polybrene (Sigma), and used to transduce HaCaT cells overnight [75] ...
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bioRxiv - Physiology 2024Quote: ... Primary antibodies were anti-Iba-1 (Wako, 019-1971, 1:500) and NeuN (Millipore, MAB377, 1:500). For immunofluorescent staining ...
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bioRxiv - Cell Biology 2024Quote: ... for 10 min then stained with 1% Rhodanile Blue (1:1 mixture of Rhodamine B (Sigma, R6626) and Nile Blue chloride (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... and 1:1 (v:v) mixture oil of hexadecane (Sigma-Aldrich) and silicon oil (Sigma-Aldrich) ...
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Tuning trophoblast invasion in a gelatin hydrogel via soluble cues from the maternal-fetal interfacebioRxiv - Bioengineering 2020Quote: ... per 1 mL 1% bovine serum albumin (Sigma-Aldrich, A4503) in PBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% cyclocreatine (w/v) (2-imino-1-imidazolidineaceticacid) (Sigma #377627) was supplied in drinking water ad libitum for one month.
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bioRxiv - Cancer Biology 2021Quote: ... 1% cyclocreatine (w/v) (2-imino-1-imidazolidineaceticacid) (Sigma #377627) in drinking water was started one week after tumour cell implantation ad libitum for one month ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG (Sigma-Aldrich, F1804, IF 1:100, WB 1:1000) and MKLP1 (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... Rab14 (Sigma-Aldrich, R0656, IF 1:100, WB 1:500), FLAG (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... 100 μl of “overlay” (1:1 of 2% methylcellulose (Sigma) and culture media ...
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bioRxiv - Plant Biology 2022Quote: ... Seedlings were incubated with 1 mg·mL-1 DAB (Sigma-Aldrich) in 20 mM 2-ethanesulfonic acid (MES ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-MLCK (Sigma Aldrich, HPA031677, 1:700-1:1000), rabbit anti-CNN1 (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... Tubulin beta (1:8,000; clone KMX-1 from Merck Millipore), dMi-2 (1:8,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... METTL14 (Sigma-Aldrich HPA038002, WB: 1:5000, IF: 1:100), WTAP (Proteintech 60188 ...
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bioRxiv - Molecular Biology 2020Quote: ... ALKBH5 (Sigma-Aldrich SAB1407587, WB: 1:250, IF: 1:100), β-Actin (Sigma-Aldrich A5441-100UL ...
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bioRxiv - Neuroscience 2019Quote: ... anti-vGlut (Merck Millipore, cat MAB5502, 1:200; 1:1,000); anti-GluR2 (Merck Millipore ...
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bioRxiv - Cell Biology 2019Quote: ... tubulin (clone 5-B-1-2 from Sigma, 1:5000), GST (clone 8-326 ...
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bioRxiv - Developmental Biology 2019Quote: ... with 100 μg ml−1 ampicillin/1 mM IPTG (Sigma). Empty L4440 RNAi vector served as a control ...
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bioRxiv - Biophysics 2019Quote: ... 1:500 for mouse monoclonal anti-Tau-1 (Millipore MAB3420), 1:500 anti-chicken tau (Genetex GTX49353) ...
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bioRxiv - Cell Biology 2020Quote: ... and 1% phosphatase inhibitor cocktails 1 and 2 (Sigma-Aldrich) added fresh] for 10 min on ice and proteins were resolved by SDS-PAGE for western blotting.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... while 250 µl of cold (1:1) chloroform:methanol (Sigma Aldrich) was added to the sample ...
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bioRxiv - Microbiology 2021Quote: ... 100 μl of “overlay” (1:1 of 2% methylcellulose (Sigma) and culture media ...
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bioRxiv - Cell Biology 2021Quote: ... blocked for 1 h in 1% BSA (Sigma, Cat# A7906)/0.1% Triton X-100/PBS (blocking solution) ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-mouse (Sigma-Aldrich, A9917, 1:3,000 or 1:4,000), anti-rabbit (Life Technologies ...