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Citations for Millipore Sigma :
4001 - 4050 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: Limbs from Mig-6 over/over and control mice were harvested and fixed in 4% paraformaldehyde (Sigma) for 24 hours and decalcified in ethylenediaminetetraacetic acid (5% EDTA in phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... 6-8 weeks-old mice were intraperitoneally injected with 0.1mL of 10mg/mL 4-hydroxytamoxifen (Sigma Aldrich) dissolved in a solution of DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were left to dry completely before being mounted with 6 µl of Mowiol 4-88 (Sigma) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... were placed in the middle of a 2% agar plate containing a container with 10 µl n-amylacetate (AM, diluted 1:50 in mineral oil; SAFC) or 3-Octanol (3-Oct, Sigma) on one side and a blank on the other side ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then fixed with 4% p-formaldehyde (PFA) during 15 min and stained with DAPI (5 min, 1 μg/ml in PBS; Merck-Millipore).
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bioRxiv - Evolutionary Biology 2021Quote: ... Three more washing steps with TBS were performed before resolving the staining with 5 ml of substrate per membrane: a 30 mg tablet of 4-chloro-1-naphthol (Sigma) dissolved in 10 ml of methanol ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at 4°C overnight with the following primary antibodies in 5% BSA in TBST: puromycin (Sigma, 1:25,000) and vinculin (Cell Signaling Technology ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... Membranes were incubated in primary antibodies (1:1000) overnight at 4° in 5% bovine serum albumin (BSA) (Sigma, A9647-100G), washed with TBST ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed in 4% PFA and stained with a phospho-Histone H3 primary antibody (1:1000; Millipore #5-806) overnightat 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... The reproductive organs were exposed by removing the sepals and placed into glass capillaries (size 4, Zeiss) containing ½ MS medium (5% sucrose, pH 5.8) with 1% low melting point agarose (Sigma Aldrich). The inflorescence embedded in solidified MS was pushed out of the capillary and then placed into the capillary holder for the Z.1 ZEISS light-sheet microscope ...
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bioRxiv - Biochemistry 2022Quote: ... and phosphatases (1.15 mM sodium molybdate, 4 mM sodium tartrate dihydrate, 1 mM freshly prepared sodium orthovanadate, 5 mM glycerophosphate, all Sigma), and 100 mM N-Ethylmaleimide (NEM ...
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bioRxiv - Molecular Biology 2022Quote: ... for one hour and incubated overnight at 4°C in 5% milk/PBS-T with the following antibodies: anti-LIMCH1 (1:400, catalog number HPA063840, Sigma), anti-MBNL1 (1:1,000 ...
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bioRxiv - Biophysics 2023Quote: ... product of fusion protein Bre1(591-700) -(GGS)4- Bre1(591-700) -(GGS)5-Rad6(1-172) was cloned into vector pET32-a (Novagen) with N-terminal thioredoxin ...
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bioRxiv - Microbiology 2023Quote: ... 5 mg/mL 6-animopenicillanic acid (6-APA) (Sigma-Aldrich), 5 mg/mL 7-aminocephalosporanic acid (7-ACA ...
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bioRxiv - Biochemistry 2022Quote: ... Cholesteryl hemisuccinate (CHS, C6013) and 1-stearoyl-2- hydroxy-sn-glycero-3-phosphocholine (Lyso-PC) were purchased from Sigma. Brain phosphatidylinositol-4-phosphate (PI(4)P) ...
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bioRxiv - Neuroscience 2021Quote: ... frozen tissue (from 2–3 mice or 1 rat) was homogenized in Lysis Buffer (EZ Prep Nuclei Kit, Sigma) with Protector RNAase Inhibitor (Sigma ...
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bioRxiv - Neuroscience 2022Quote: Recordings were then performed in oxygenated aCSF superfused at a rate of 2-3 ml/min and supplemented with 100 µM picrotoxin (Alomone Labs) and 0.5-1 µM Strychnine (Sigma). The internal solution was the same as above with 2-4 mM QX314 to block Na+ channels from driving AP firing ...
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bioRxiv - Cell Biology 2023Quote: ... 1 3 v/v) at 80°C for 2 h after addition of 50 nmol 5α-Cholestane (Sigma, C8003) as internal standard for neutral sterol analysis ...
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bioRxiv - Microbiology 2023Quote: ... and AP2XII-1-AID-HA /AP2XI-2-mAID-MYC was achieved with 3-indoleacetic acid (IAA, Sigma-Aldrich # 45533). A stock of 500 mM IAA dissolved in 100% EtOH at a ratio of 1:1,000 was used to degrade mAID-tagged proteins to a final concentration of 500 μM ...
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bioRxiv - Molecular Biology 2021Quote: ... chromosome spreads were obtained following 4 h incubation in colchicine 5 μM (Sigma-Aldrich) and prepared following standard procedure consisting of treatment with a hypotonic solution (75 mM KCl ...
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bioRxiv - Neuroscience 2021Quote: 4-5 week old mice were injected with 100 mg/kg BrdU (Sigma-Aldrich) intraperitoneally once every 3 days for 6 total doses ...
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bioRxiv - Cell Biology 2022Quote: ... at passages 4-5 were cultured on Millicell EZ 8-well glass slides (Millipore) pre-coated with gelatin (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... Briefly sections (5 µm) were fixed in 4% Paraformaldehyde (Sigma Aldrich, Cat. No. P6148) in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Müller’s Organs were fixed overnight at 5°C in 4% paraformaldehyde (P6148, Sigma Aldrich) dissolved in Phosphate Buffer Saline (PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were pretreated with 4% PFA in PBS and 5% Donkey serum (Sigma, #D9663) in PBS with 0.1% Triton-X100 ...
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bioRxiv - Cell Biology 2021Quote: ... we mounted the samples in Mowiol 4-88 mounting media (Sigma 9002-89-5). All images were acquired using a 63x 1.4 NA HC Plan Apochromat oil objective on a Leica SP8 confocal microscope ...
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bioRxiv - Cell Biology 2021Quote: ... for 5 minutes followed by fixation with 4% paraformaldehyde solution (Sigma, Cat. No. 158127) freshly made in 10 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... for 5 mins and fixed with 4% paraformaldehyde (Agar scientific)/ 0.0075% glutaraldehyde (Sigma Aldrich) in 1X PBS for 30 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 4 min with 5% w/v sodium carbonate (S7795, Sigma-Aldrich) in 3.7% neutral buffered formaldehyde ...
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bioRxiv - Developmental Biology 2022Quote: ... incubated overnight at 4°C in blocking solution composed by 5% BSA (Sigma-Aldrich) or 5% Non-Fat Dry Milk in PBS-TWEEN 0,1% (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... tissue sections of 4-5 μm thickness were collected on poly-L-lysine (Sigma) coated slides using a microtome and stained according to standard protocols ...
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bioRxiv - Immunology 2023Quote: Zebrafish embryos at 24 hpf were treated with 5 μM 4-OHT (H7904, Sigma) for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... the muscles were mounted in Mowiol 4-88 mounting media (Sigma 9002-89-5). To image the muscle ultrastructure ...
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bioRxiv - Developmental Biology 2023Quote: 5 dpf embryos were euthanised in 4 mg/ml MS-222 (Sigma-Aldrich, E10521) solution ...
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bioRxiv - Cancer Biology 2024Quote: ... 100mg/mL penicillin,100mg/mL streptomycin and 5 μM 4-hydroxytamoxifen (H7904 Sigma, UK). For all experiments ...
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bioRxiv - Bioengineering 2024Quote: ... and 4 ng/L 3,3′,5-triiodo-L-thyronine sodium salt (T6397, Sigma-Aldrich). The cells were cultured in a 75 cm2 flask (658175 ...
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bioRxiv - Neuroscience 2024Quote: ... spun at 2.000 g for 5 min at 4°C and Benzonase (Sigma-Aldrich) added at a concentration of 50U/mL to supernatants for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 5’-GATCCCCAAATCT-3’ and 5’-GATCAGAT[BtndT]TGGG-3’ with 5’ end phosphate (Sigma-Aldrich), were annealed (81 µl of each Oligo 100 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... 5’-GATCCCCAAATCT-3’ and 5’-GATCAGAT[BtndT]TGGG-3’ with 5’ end phosphate (Sigma-Aldrich), were annealed (81 µl of each Oligo 100 µM ...
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Hypothalamic melanocortin-4 receptors on astrocytes mediate inflammation and body weight homeostasisbioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich) prior to blood draw via cardiac puncture followed by transcardial perfusion with 10 mL of 10% sucrose in 1X Phosphate Buffered Saline (PBS) ...
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bioRxiv - Biophysics 2021Quote: ... The enzyme/protectant mixture was added 1:10 to assay buffer (100 mM Sodium Phosphate, 2 mM Sodium Pyruvate [Sigma-Aldrich], 1 mM NADH [Sigma-Aldrich], pH 6). Enzyme kinetics were measured by NAD+ absorbance at 340 nm ...
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bioRxiv - Plant Biology 2020Quote: ... 2-3-week-old plants were treated with 150µM DCMU (D2425-100G, Sigma), kept for 1 hour in the dark and then exposed to high light (HL ...
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bioRxiv - Cell Biology 2020Quote: ... TRCN0000037282 (shRNA Trim39#2) and TRCN0000438509 (shRNA Trim39#3) were from Sigma-Aldrich. Lentiviral particles were produced as previously described (Iréna Lassot et al. ...
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bioRxiv - Immunology 2021Quote: ... mtDNA depletion was induced by 100 μM 2′,3′ dideoxycytidine (ddC, Sigma-Aldrich) in medium supplemented with uridine and sodium pyruvate ...
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bioRxiv - Molecular Biology 2022Quote: ... growth medium was supplemented with 100 μM of 2′,3′-dideoxycytidine (Sigma; D5782) or 50 ng/ml of ethidium bromide ...
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bioRxiv - Neuroscience 2022Quote: ... and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2, Phosphatase Inhibitor Cocktail 3, Sigma-Aldrich), and then incubated for 1 hour on ice ...
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bioRxiv - Neuroscience 2024Quote: ... 3-month-old male and female mice were anesthetized with 2% avertin (Sigma) and positioned in a stereotaxic apparatus (David Kopf instruments) ...
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bioRxiv - Neuroscience 2024Quote: ... P8340) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich, P5726 and P0044). Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... Phosphatase inhibitor cocktail 2 (#P5726) and cocktail 3 (P0044) were from Sigma-Aldrich. Glutathione Sepharose 4B (#17-0756-01 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation in a solution of 2/3 dichloromethane (DCM; Sigma-Aldrich), 1/3 MetOH ...