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Citations for Millipore Sigma :
3951 - 4000 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 15% (w/v) Kolliphor HS 15 (Sigma, Cat. 42966), and 80% normal saline.
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bioRxiv - Molecular Biology 2024Quote: ... incubated in 4% w/v BSA (Sigma-Aldrich, A2153)/TN buffer for 1-2 hours at room temperature in a humid box.
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bioRxiv - Cell Biology 2024Quote: ... 10mM Complex V buffer (0,1M Tris (Sigma-Aldrich #T1503), 0,01M MgCl2 (Sigma-Aldrich #M9272) ...
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bioRxiv - Microbiology 2021Quote: ... Cells (~106/ well) were seeded into wells of a 24-well dish and activated with 25 nM phorbol myristate acetate (PMA) (Sigma-Aldrich, MO, USA) for 10 minutes ...
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bioRxiv - Biophysics 2020Quote: ... except comparison of TSG101 mutants proceeded by 6-well plates (9.4 cm2 wells, Sigma). The NEB Gaussia and Cypridina plasmids were used at 40 ng each per well for each 96-well assay (or ten times as much for 6-well plates) ...
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bioRxiv - Cell Biology 2022Quote: ... HESCs at 2 × 105 per well were plated in 6-well dishes (Sigma-Aldrich), with BM-MSCs or UC-MSCs in the inner wells with 0.4 µm pore size inserts ...
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bioRxiv - Cell Biology 2022Quote: ... 2 × 105 HESC cells per well were plated in 6-well dishes (Sigma-Aldrich). As performed for the proliferation assays ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS and plated in a well of an 8-well EZ Slide (Millipore) which had been pre-coated with 5 ul Matrigel ...
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bioRxiv - Cell Biology 2020Quote: Human HeLa229 cells (ATCC CCL-2.1tm) and Sam50 knockdown cells sam50kd-225 were cultured in 10% (v/v) heat inactivated FBS (Sigma-Aldrich, St. Louis, USA) RPMI1640 + GlutaMAXtm medium (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... The minced kidneys were digested for 8 minutes at 37C in 2mL of 0.25% w/v Collagenase A/1% w/v Pancreatin (Sigma-Aldrich; Cat. 101378001, P1750), with manual dissociation via pipetting through a P1000 tip every 2 minutes ...
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bioRxiv - Genomics 2020Quote: DNA from sorted drops was recovered by breaking the emulsion with 10% (v/v) solution of perfluoro-octanol (Sigma-Aldrich, catalog no. 370533) and addition of 20 μl DI water ...
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bioRxiv - Bioengineering 2019Quote: ... Settling of cells within syringes was minimized by matching the density of the medium to the cell suspension using a 36 % v/v Optiprep Density Gradient Medium (Sigma-Aldrich, Buchs, Switzerland). A commercial calibration kit (Quantum Alexa Fluor 488 MESF ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The supernatant was discarded and pellet was resuspended in 100 μL ice-cold lysis buffer (50 mM K2HPO4, 1 mM EDTA, pH 6.5 and 0.1 % v/v Triton X-100, Sigma-Aldrich, St. Louis, MO, USA). The cells were incubated on ice for 15 minutes with gentle tapping from time to time ...
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bioRxiv - Plant Biology 2019Quote: ... the bacteria were plated on LB agar plates (2.5% w/v LB broth, Prod. No. L3152; 1.5% w/v agar, Prod. No. 05039, Sigma-Aldrich Co., St. Louis, USA) and incubated at 28°C ...
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bioRxiv - Neuroscience 2021Quote: ... This fixation step and the subsequent primary and secondary antibody incubations were each followed by several washes with PBT (0.5 % v/v TX-100, Sigma Aldrich: X100, in PBS). To block nonspecific antibody binding ...
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bioRxiv - Cell Biology 2021Quote: ... 2.7 mM KCl [Sigma-Aldrich P9541], 19 mM Tris-HCl pH 7.5, and 0.1% v/v Tween 20 [Sigma-Aldrich P7949] in water) for 1 hr at room temperature ...
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bioRxiv - Immunology 2022Quote: ... mice were immunized at weeks 0 and 4 with 5 µg of gH/gL monomer or 60-mer formulated in PBS (monomer) or TBS (60-mer) with 50% (v/v) Sigma Adjuvant System (SAS; Sigma Aldrich, catalog #S6322) for a total volume of 100 µL ...
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bioRxiv - Biophysics 2022Quote: ... the pancreas was transferred to a foil-covered glass vial containing a 1:1 solution of MeOH (100%) and BABB (1:2 (v/v) benzyl alcohol (402834-500ml, Sigma-Aldrich, Gillingham, UK) to benzyl benzoate (B6630-1L ...
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bioRxiv - Plant Biology 2021Quote: ... total soluble protein was extracted from 50 mg of ground frozen shoot material in 250 µl 2× Laemmli buffer supplemented with 1% (v/v) phosphatase inhibitor cocktail 2 (Sigma-Aldrich, Steinheim, Germany). Proteins were denatured for 10 min at 90°C and separated using 10% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... containing 1% (w/v) sucrose unless indicated otherwise and solidified with 1% (w/v) EDTA-washed Agar Type M (Sigma-Aldrich, Steinheim, Germany), as described (Schulten et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 4°C and incubated for 10 min at 37°C in 0.5ml trypsin-EDTA (0.25% trypsin w/v and 0.1% EDTA w/v, Sigma 59418C diluted 1:1 in DPBS). 5 ml HBSS with 10% (v/v ...
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bioRxiv - Biophysics 2020Quote: ... ammoniumpersulfate and 0.1 % (v/v) N,N,N,N-tetramethylethylenediamine onto freshly plasma-cleaned cover slips that were treated with 3-aminopropyltriethoxysilane (Sigma, Munich, Germany, A3648) and subsequently with a 0.05% glutaraldehyde solution (Sigma ...
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bioRxiv - Biophysics 2019Quote: ... Permeabilization of the membrane and blocking of unspecific binding was achieved by adding 5 % (w/v) bovine serum albumin (IgG-free, Carl Roth, Karlsruhe, Germany) and 0.3 % (w/v) Triton-X 100 (Sigma-Aldrich, St. Louis, USA) in PBS for 30 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... were done in Balb/c mice (n=4 in each group) through intraperitoneal (ip) injections of 20 μg of antigen in 200 μL of 50% v/v of adjuvant (Sigma Adjuvant System®). For experiments in Fig 4A ...
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bioRxiv - Bioengineering 2020Quote: We then prepared and autoclaved a 2% w/v alginate (Pronova UltraPure MVG, NovaMatrix, Drammen, Norway) or 7.5% w/v alginate (Alginic acid sodium salt, Millipore Sigma, St Louis, MO) in HEPES saline buffer solution (Ultrasaline A ...
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bioRxiv - Plant Biology 2022Quote: ... 2 volumes of stop solution—composed of 1:1 (v/v) of acetonitrile and isopropanol with 0.1% formic acid and 1 µM telmisartan as internal standard (Sigma-Aldrich, St. Louis, MO)—were added to the assays and mixed by pipetting ...
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Ultra-high-throughput microbial single-cell whole genome sequencing for genome-resolved metagenomicsbioRxiv - Microbiology 2022Quote: ... The microdroplets comprising amplified microbial genome and a precursor hydrogel solution containing 40% (v/v) Acrylamide solution (Sigma-Aldrich, catalog no. A4058-100ML) and 20% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... All tissue culture basal media was supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), 100 IU penicillin (Gibco® ...
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bioRxiv - Developmental Biology 2022Quote: ... organoids were cultured in ENRWN medium, which includes ENR medium with 50% v/v WNT3A (CM, ATCC CRL-2647) and 10 mM Nicotinamide (Sigma-Aldrich, N1630-100MG). For post-infection cultures ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... v/v) An aliquot corresponding to 1 mg of tissue was sampled and 2 µg TG17 (glyceryl triheptadecanoate, Sigma Aldrich, les Ulis, France) added as internal standard to verify the completeness of hydrolysis ...
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bioRxiv - Molecular Biology 2024Quote: ... were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA), at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Molecular Biology 2024Quote: ... for MCF-7/MDA-MB-231/ARPE-19/HEPG2/MCF10A cells and 10% (v/v) bovine calf serum (Sigma-Aldrich, Cat. No. 12133C) for NIH3T3 cells ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 mL of a UPLC-grade water:methanol (3:1, v/v) solution with 1:500 diluted 13C-and 15N-labeled amino acids standard mix (Sigma-Aldrich, Merck, Darmstadt, Germany) was added to the tubes ...
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bioRxiv - Immunology 2024Quote: ... pH 7.6) + 0.05% v/v Triton-X100 and 10% donkey serum and subsequently incubated with rat-MLKL (Millipore MABC604 clone 3H1 1:1000) overnight ...
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bioRxiv - Biochemistry 2023Quote: ... The viral transduction was accomplished by incubating cells with a mixture of 10% (v/v) viruscontaining media and complete growth media in the presence of Polybrene (10 μg/mL, Sigma TR-1003-G) for 48 hours ...
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bioRxiv - Bioengineering 2023Quote: ... The following day the tissues were incubated in primary antibody diluted 1:100 in fresh block buffer and 5% (v/v) dimethyl sulfoxide (DMSO; Sigma; a penetration enhancer) at 37°C for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... larvae were washed thrice with PBST for 5 min and re-blocked with PBST containing 5% (v/v) Goat Serum (Sigma-Aldrich, Gillingham, UK) for 2 h ...
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bioRxiv - Immunology 2023Quote: ... 6X SDS-PAGE sample loading buffer (G Biosciences, St. Louis, MO #785-701) containing 5% v/v 2-mercaptoethanol (Sigma-Aldrich #M3148-100ML) was added to lysates ...
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bioRxiv - Cancer Biology 2023Quote: HCC38 cells were purchased from ATCC and cultured in RPMI containing 10% (v/v) FBS (PS; 5.000 units penicillin and 5 mg streptomycin/ml in H2O, Sigma Aldrich, cat no. P4333). Cells were routinely tested to confirm the absence of mycoplasma contamination ...
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bioRxiv - Genomics 2023Quote: ... The culture medium was then aspirated and cells were lysed by adding 600ul buffer RLT from RNeasy Mini kit with 1:100 V/V β-mercaptoethanol (Sigma M3148-250ML) to the dish ...
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bioRxiv - Plant Biology 2023Quote: ... 5 to 10 dry seeds by accession were individually and synchronously disposed on perfectly flat 0.6 % (w/v) agarose media containing 0.00004 % (w/v) of toluidine blue O polychromatic stain (Sigma-Aldrich, Saint-Quentin-Fallavier, France). Upon the contact with the aqueous media ...
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bioRxiv - Plant Biology 2023Quote: ... 10 μM proteasome inhibitor MG132 (Sangon Biotech, product no. T510313, Shangai, China) and 1% (v/v) protease inhibitor cocktail for plant extracts (Sigma, product no. P9599). The supernatant was mixed with loading buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... the two were mixed gently at a ratio of 1:2 (v/v) and resuspended in 20 times ice-cold Collagen (Sigma, Cat # C0130-1G). Cells were then aliquoted in 20 µl volumes into a 48-well plate and allowed to polymerize at 37□°C for 45Lmin ...
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bioRxiv - Cancer Biology 2023Quote: ... in Millipore water supplemented with 1 % (v/v) protease and phosphatase inhibitor cocktail (Protease and Phosphatase Inhibitor Cocktail, Sigma-Aldrich, cat. no. PPC1010). Homogenization was carried out in different rounds ...
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bioRxiv - Cancer Biology 2023Quote: Post EdU or TUNEL stained larvae were washed thrice with PBST for 5 min and re-blocked with PBST containing 5% (v/v) Goat Serum (Sigma-Aldrich, Gillingham, UK) for 2 h ...
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bioRxiv - Plant Biology 2024Quote: ... followed by a final wash using the LiCl wash buffer (0.25 M LiCl, 1% (v/v) NP-40 (Sigma-Aldrich NP40S-100 mL), 1% Sodium Deoxycholate ...
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bioRxiv - Microbiology 2021Quote: ... wells of ELISpot plates (Millipore) were coated overnight with purified soluble pre-F protein (30 ng/well in PBS) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 384-well maxisorp plates (Sigma) were coated with NAV ...
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bioRxiv - Immunology 2021Quote: ... Ninety-six-well plates (Millipore) were coated with 100 μl of 10 μg/ml anti-human IFN-γ antibody (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... as well as DAPI (Sigma) for nucleus staining.