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Citations for Millipore Sigma :
3951 - 4000 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... human iPSCs were cultured on gelatin-coated dishes in differentiation medium (GMEM (Sigma) supplemented with 1mM sodium pyruvate ...
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bioRxiv - Biochemistry 2023Quote: ... Zymosan A was opsonized by incubation with human serum (Sigma-Aldrich, no. H4522) at 37 °C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... which was the concentration used for the human IgG negative control (Sigma, I2511) in subsequent experiments ...
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bioRxiv - Microbiology 2024Quote: ... MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Customised 7-plex Panel (Merck Millipore) was used to quantify cytokine levels.
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bioRxiv - Biophysics 2024Quote: ... We obtained the antibodies through commercial suppliers: IgA from human serum (Sigma-Aldrich), IgM from human serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... MILLIPLEX Map Human cytokine/chemokine Magnetic Bead Panel – Premixed 41 Plex (Millipore Sigma), run on a MAGPIX® xMAP instrument according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Fresh or cryopreserved primary adult human hepatocytes (BeCytes Biotechnologies, BiolVT or Sigma-Aldrich) were resuspended in hepatocyte spheroid medium (HSM) ...
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bioRxiv - Neuroscience 2024Quote: Human spinal cord samples were homogenized with a dounce homogenizer (Sigma Aldrich, D8938) in 1mL nuclei lysis buffer using the Nuclei Pure Prep Nuclei Isolation Kit (Sigma Adlrich ...
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bioRxiv - Cell Biology 2024Quote: ... primary human skeletal muscle cells were seeded and differentiated on laminin (Sigma Aldrich) coated 4-well Nunc Lab-Tek II Chamber Slides (Thermo Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... and 100 U/mL premium grade recombinant human IL-2 (Sigma catalog #I7908) with media changes every 2-3 days to maintain the cells at a concentration of 0.2 M cells/mL.
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bioRxiv - Immunology 2024Quote: ... coated with human placental type IV collagen (Sigma-Aldrich, St. Louis, MO, USA). The cells were cultured using Ex Plus in both the basolateral and apical compartments for 1 week ...
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bioRxiv - Microbiology 2024Quote: Purified human PMNs were incubated with phorbol 12-myristate 13-acetate (PMA) (Sigma) 100nM for 4h ...
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bioRxiv - Microbiology 2024Quote: ... Coverslips with C3bi-coated beads were incubated with rabbit anti-human IgM (Sigma) at 1:250 ...
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bioRxiv - Microbiology 2022Quote: ... The SPA-tagged Rho protein was visualized by hybridization with the primary mouse ANTI-FLAG M2 monoclonal antibodies (Sigma-Aldrich; dilution 1:5,000) and the secondary goat peroxidise-coupled anti-mouse IgG antibodies A2304 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... An even protein transfer was verified by reversibly staining membranes with Poncaeu S solution (Sigma-Aldrich, 0.1% w/v in 1% acetic acid). Next ...
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bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
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bioRxiv - Neuroscience 2020Quote: ... Dendrites were identified using a staining against the microtubule-associated-protein-2 with Rabbit-anti-MAP2 PAB (Milipore Sigma, cat. AB5622, 1:1000) and GAR-STAR488 SAB (Abberior ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Molecular Biology 2021Quote: ... the antibody – lysate mixture was incubated at 4°C with rotation for 1 hr prior to the addition of protein A/G agarose beads (Millipore, 50 L/IP) and continued overnight incubation ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight while shaking at 4°C in the blocking solution that contained combinations of the following primary antibodies: mouse anti- three microtubule-binding domain tau protein (3R-tau, 1:500, Millipore, Cat# 05-803), rabbit anti-purkinje cell protein 4 (PCP4 ...
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bioRxiv - Microbiology 2022Quote: ... THP-1 cells were harvested and washed twice with PBS and lysates were prepared using CytoBuster protein extraction reagent (Novagen/EMD, Gibbstown, NJ) supplemented with complete mini EDTA-free protease inhibitor (Roche ...
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bioRxiv - Cancer Biology 2019Quote: CAF cell monolayers were lysed for protein with RIPA buffer containing protease inhibitor cocktail at 1:100 dilution (both Sigma-Aldrich, Dorset, UK). Lysed cell suspensions were quantified for protein concentration using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The precipitated proteins were centrifuged for 10 min at 18,000 g and the protein pellet resuspended 50 mM NH4HCO3 before digestion with sequencing grade porcine trypsin (enzyme to protein ratio of 1:40) (Sigma-Aldrich, MO, USA) for 16 hours at 37°C ...
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bioRxiv - Genomics 2019Quote: ... Approximately 25μg of proteins were analyzed on 10% SDS-PAGE followed by western blots using monoclonal anti-FLAG-M2-Peroxidase (HRP) (Sigma Aldrich, 1:2000) and anti-β actin (1:4000).
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bioRxiv - Biochemistry 2021Quote: ... 25 µg of total protein were separated by denaturing PAGE and subjected to Western blotting using mouse monoclonal anti-FLAG antibody (M2, Sigma Aldrich, 1:1000) followed by probing with an HRP-coupled anti-mouse light chain-specific secondary antibody (1:10000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were blocked and incubated with specific antibodies: HA-tagged spike protein was detected with anti-HA antibody (H9658, Sigma-Aldrich, 1:10,000), hACE2 was detected with anti-ACE2 antibody (sc-390851 ...
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bioRxiv - Biochemistry 2020Quote: A 10 μM solution of purified PAPR10-CD protein was incubated for 20 minutes at 37°C with 1 mM final concentration of β-Nicotinamide Adenine Dinucleotide (β NAD+) (Millipore-Sigma) in a reaction buffer (50 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Neuroscience 2022Quote: ... The immunoprecipitated proteins were analyzed by SDS-PAGE and Western blotting using the following antibodies: anti-HA (Sigma, H3663; clone 12CA5, 1:1000), anti-Flag (Sigma ...
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bioRxiv - Neuroscience 2022Quote: Total GLP1 protein in brain tissue or in plasma was determined using GLP-1 Total ELISA 96-Well Plate Assay (Sigma-Aldrich, EZGLP1T-36K). The assay was conducted according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight while shaking at 4◦C in the blocking solution that contained combinations of the following primary antibodies: mouse anti-3 microtubule-binding domain tau protein (3R-Tau, 1:500, Millipore, Cat# 05-803), rabbit anti-Purkinje cell protein 4 (PCP4 ...
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bioRxiv - Microbiology 2024Quote: ... a 10 μM solution of purified PAPR10-CD protein was incubated for 20 minutes at 37°C with 1 mM final concentration of β-Nicotinamide Adenine Dinucleotide (β NAD+) (Millipore-Sigma) in a reaction buffer (50 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: ... separate sets of sections were immunohistochemically processed using anti-glial fibrillary acidic protein (GFAP) monoclonal antibody 1:500 (G3893, Sigma, Saint Louis, Missouri), anti-doublecortin (DCX ...
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bioRxiv - Physiology 2023Quote: ... We blocked sections in 5% normal goat serum in phosphate-buffered saline with 0.1% Tween 20 (blocking buffer) for 30 min at room temperature (RT) and then incubated in primary antibody for laminin protein (1:500, Sigma catalog no. L93931) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was detected using the Simple-Western-JESS capillary western blot system (Bio Techne) multiplexed for total protein and chemiluminescence detection of mouse αSFPQ (1:2000; Sigma-Aldrich WH0006421M2, RRID:AB_1843565) or mouse αTIAL1 (1:10 ...
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bioRxiv - Immunology 2023Quote: ... For purification of HDAC1-Flag 1 mg of protein was mixed with 120 µl of crosslinked M2-Flag beads (Sigma Aldrich, M8823-5ml) and incubated rotating overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Knockdown of proteins was achieved through RNA interference either by CaCl2 transfection of gene-specific shRNA encoding vectors (pLKO.1; Sigma-Aldrich or shRNAmiR) on DIV 7 or by transduction of cells with lentiviral particles harboring the gene-specific shRNA on DIV 2 ...
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bioRxiv - Neuroscience 2023Quote: Western blot analysis of MBP was performed on protein lysates from the anterior cerebrum at P22 as previously described [32] using rat anti-MBP (Millipore MAB386 1:500) and anti-β-actin (Sigma-Aldrich AC-74 ...
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bioRxiv - Cell Biology 2020Quote: ... For visualization of blue protein bands from the prestained protein ladder the anti-BLUE antibody (Anti-BLUE, 2D2-F11, Millipore) was used (Schuchner et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions containing dCas9 protein were pooled and the protein was concentrated with spin concentrators (Amicon Ultra 15, MWCO 10k; Millipore), and after adding glycerol to 20% final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... protein concentration was routinely determined by densitometry of Sypro-Orange stained gels against a calibration curve with protein standards (Sigma) using ImageJ ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using centrifugal filters and concentrations were measured using the Bicinchoninic acid (BCA) protein assay kit (Millipore Sigma). Proteins were aliquoted and stored at −80°C.
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bioRxiv - Developmental Biology 2020Quote: ... Fifty or thirty-five (depending on the protein detection) micrograms of proteins was separated on 9% Tricine gels and transferred to polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated separately with anti-Mmp-9 (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein extracts were then mixed with the indicated primary antibodies and protein A/G agarose (Sigma, St. Louis, MO, USA) or anti-FLAG M2 agarose for overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... For the ChIP using SV40 chromatin isolated 30 minutes post-infection we substituted a mixture of protein A and protein G magnetic beads (16-663, Millipore) for the protein A and protein G agarose which we previously used in our publications ...
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bioRxiv - Cell Biology 2019Quote: ... Gene Tex) antibody was incubated with protein G coated magnetic beads (PureProteome protein G magnetic beads, LSKMAGG10, EMD Millipore Corp.) for 1-3 hrs in IP (immunoprecipitation ...
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bioRxiv - Cell Biology 2021Quote: For conjugation of SNAP-tagged proteins (paxillin, kindlin) recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼50 μM ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins and input proteins were analyzed by immunoblotting with α-HA or α-FLAG antibodies (Sigma-Aldrich, Cat # A8592).
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bioRxiv - Microbiology 2020Quote: ... Protein-protein interactions in situ were visualized using the Duolink® in situ proximity ligation assay (PLA) system (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Proteins smaller than 30 kDa were removed by passing the protein lysate through an Amicon Ultra - 15 Centrifugal Filter (Millipore). One mg of cell lysate in 500 μL lysis buffer was pre-cleared by adding 10 μL of protein A/G PLUS-agarose beads (Santa Cruz ...