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Citations for Millipore Sigma :
3951 - 4000 of 10000+ citations for 2 Aminomethyl 1 3 thiazole 4 carboxylic Acid Hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... Sonication was followed by 2 cycles of bead beating (200 µL glass beads, 400 nm acid washed, Sigma), each cycle lasting 20 sec at 4 Mz/sec with 1 min cooling intervals between the cycles.
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bioRxiv - Microbiology 2022Quote: ... Thiol concentration at the same intervals as cells infected with virus was determined by Ellman’s reagent (5,5’-dithio-bis-2-nitrobenzoic acid, DTNB, Sigma) by reading the optical density at 412 nm in a plate reader (Biotek Cytation 5 ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Genetics 2022Quote: ... Yeast cells were cultured in synthetic complete (SC) medium (2% D-galactose, 0.67% yeast nitrogen base without amino acids, 0.2% drop out (Sigma), (0.0076% uracil ...
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bioRxiv - Biochemistry 2022Quote: ... DHA was conjugated to fatty acid-free BSA (2% w/v; Sigma-Aldrich Pty Ltd, Sydney, NSW, Australia) in media to increase both its solubility and bioavailability (30) ...
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bioRxiv - Cell Biology 2023Quote: ... 15 U/mL of glutathione reductase and 40 μg/mL of 5,5’-dithiobis(2-nitrobenzoic acid) (Sigma-Aldrich) were dissolved in assay buffer (100 mM K2HPO4/KH2PO4 ...
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bioRxiv - Neuroscience 2023Quote: All samples were made to pH 6.0 in 50 mM 2-(N-morpholino)ethanesulfonic acid (MES) buffer (Sigma), using potassium as a counterion ...
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bioRxiv - Neuroscience 2023Quote: All samples were made to pH 6.0 in 10 mM 2-(N-morpholino)ethanesulfonic acid (MES) buffer (Sigma), using potassium as a counterion and containing 10% D2O ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were incubated in the presence of 5,5’-dithio-bis-[2-nitrobenzoic acid] (DTNB, 0.73 mM) (Sigma-Aldrich), EDTA (4 mM ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), equilibrated in lysis buffer and incubated for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Neuroscience 2024Quote: ... Oleic acid was complexed with fresh 40 % HPB (2-Hydroxypropyl)-β-cyclodextrin (H107 Sigma-Aldrich, St Louis, USA). 9 nmols of oleic acid/dose and 2 µl/dose were injected ...
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bioRxiv - Cell Biology 2024Quote: ... the medium was switched to High Glucose DMEM medium containing 2 μM retinoic acid (RA; R2625, Sigma, USA), 10% KSR ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.5 mM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (all Sigma-Aldrich, St. Louis, MO, USA). Cells were maintained at 37°C in an atmosphere of air supplemented with 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA) (Sigma Aldrich, St. Louis, MO, USA), LPS-RS Ultrapure (a selective TLR4 antagonist ...
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bioRxiv - Biophysics 2023Quote: ... The purified protein (in 25 mM piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES, P-1851, Sigma-Aldrich) pH 6.8 ...
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bioRxiv - Neuroscience 2023Quote: ... Stock solutions of the NMDA receptor antagonist DL-2-amino-5-phosphonovaleric acid (DL-APV; #5282; Millipore Sigma), GABAA receptor antagonist bicuculline methiodide (#14343 ...
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bioRxiv - Microbiology 2022Quote: ... SK-N-BE(2) cell differentiation was induced by 10 μM retinoic acid (RA) treatment (R2625, Sigma-Aldrich) for 5 days ...
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bioRxiv - Neuroscience 2024Quote: ... the growth medium was replaced with L-ascorbic acid phosphate 2-magnesium (Sigma-Aldrich, St. Louis, MO, US) according to the experimental design ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were lyophilized and resuspended in 50Lμl HPLC-water (Fisher Chemical) with 2% acetonitrile and 0.2% formic acid (Sigma) before LC-MS/MS analysis.
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bioRxiv - Bioengineering 2024Quote: ... 30 mM L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate resuspended in PBS (Cat: A8960-5G, Sigma-Aldrich), 1% Penicillin-Streptomycin (Cat ...
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bioRxiv - Bioengineering 2024Quote: ... The differentiation medium was supplemented with 100 µg/mL 2-Phospho-L-ascorbic acid trisodium (Sigma Aldrich, 49752) to stimulate ECM production ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5 mM palmitic acid and 0.5 mM stearic acid (Sigma). Non-supplemented plates only contained YPD and 1% Brij58 ...
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bioRxiv - Biochemistry 2020Quote: ... Acetic acid and trifluoroacetic acid (TFA) were purchased from Sigma. High-performance liquid chromatography (HPLC)–grade water and acetonitrile were purchased from Honeywell (Muskegon ...
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bioRxiv - Cancer Biology 2022Quote: ... oleic acid and octanoic acid were purchased from Sigma-Aldrich. These free fatty acids (FFA ...
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bioRxiv - Cell Biology 2024Quote: ... Reference material amino acids (certified amino acid mix, Sigma Aldrich) were used to prepare calibrator samples in the concentration range 0.2 to 25 µM by addition of internal standard and solvent as specified above ...
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bioRxiv - Developmental Biology 2022Quote: ... Standards for arachidonic acid and linoleic acid (Sigma Aldrich, USA) were used for validation of identification.
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bioRxiv - Genetics 2021Quote: ... were resuspended in 4 volumes lysis buffer (50 mM Tris-HCl pH 7.5, 1 mM dithiothreitol, 1 mM ethylenediaminetetraacetic acid, 1:400 Sigma protease inhibitory cocktail P8340 ...
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bioRxiv - Cell Biology 2021Quote: ... and RANK-L (100 ng/ml) for 4 to 6 days followed by staining for Tartrate-resistant acid phosphatase (TRAP) using a kit (Sigma) per manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... Auxin-inducible degradation of Brl1 in log-phase yeast cultures with OD600 = 0.1 - 0.2 was induced by addition of IP6 (4 μM phytic acid dipotassium salt, Sigma-Aldrich P5681) and either auxin (+auxin ...
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bioRxiv - Molecular Biology 2020Quote: ... and overlaid with 0.5 μl of the matrix solution (α-cyano-4-hydroxycinnamic acid in 50% acetonitrile/0.1% TFA; 5 mg/ml, Sigma). Peptide mass maps of proteins in Figures 4f ...
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bioRxiv - Cell Biology 2022Quote: Chemicals and solvents (analytical grade) were purchased from the following sources: α-Cyano-4-hydroxycinnamic acid 98% (CHCA) (Sigma Aldrich), 1,5-Diaminonaphthalene 97% (DAN ...
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bioRxiv - Cancer Biology 2022Quote: ... Digestion products were separated and identified via mass spectrometry with a MALDI–time-of-flight Voyager DE-Pro instrument (PE PerSeptive Biosystems) using the matrix 3,5-dimethoxy-4-hydroxy-cinnamic acid (Sigma-Aldrich).
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bioRxiv - Bioengineering 2020Quote: ... the culture medium was supplemented with 4 mM β-glycerophosphate and 50 μg/ml ascorbic acid (both from Sigma-Aldrich), starting from day 3 ...
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bioRxiv - Biochemistry 2021Quote: Chemicals and solvents (analytical grade) were purchased from the following sources: α-Cyano-4-hydroxycinnamic acid 98% (CHCA) (Sigma Aldrich), 2,5-Dihydroxybenzoic acid 98% (DHB ...
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bioRxiv - Microbiology 2020Quote: ... Each sample was spotted onto a titanium dioxide-coated plate (0.15 μL/spot) and covered with 0.15 μL matrix mixture (a saturated solution of α-cyano-4-hydroxycinnamic acid (Sigma) in 50% acetonitrile ...
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bioRxiv - Neuroscience 2022Quote: ... both followed by 150 mL Lana’s fixative (room temperature, 4% depolymerized paraformaldehyde and 14% saturated picric acid solution, Sigma Aldrich, P6744-1GA ...
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bioRxiv - Molecular Biology 2022Quote: Freshly-seeded healthy antral undifferentiated mucosoid cultures were treated every 24 h with 4 µM 5aza (in 0.02 % acetic acid; Sigma-Aldrich). A global decrease in DNA methylation ...
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bioRxiv - Biochemistry 2023Quote: Sample analyses were performed using either 4-hydroxycinnamic α-cyano acid (CHCA - Cat No. #201611-92-9, Sigma-Aldrich, USA) or 2-mercaptobenzothiazole (MBT – Cat No ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting submucosa was cut into 6 inch pieces and decellularized using 4% alcohol/0.1% peracetic acid (v/v, Sigma-Aldrich) and washed thoroughly with 1X PBS and Type 1 water followed by lyophilization and comminuted into an injectable particulate form via cryogenic milling and sieving through 425 µM pore sieve ...
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bioRxiv - Microbiology 2022Quote: ... the methanol:water solution was evaporated under a N2 stream and a 125 µM stock solution was prepared in 4 mg/mL fatty acid free Bovine Serum Albumin (Sigma) in PBS and stored at -20 ºC for up to 3 months.
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bioRxiv - Bioengineering 2024Quote: ... functionalized glass coverslips were immersed in a solution of 25 ml of anhydrous ethanol containing 20 µl of acetic acid and 0.4 mg/ml 4-nitrobenzaldehyde (4NB, Sigma Aldrich) at 50 °C for 3 h ...
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bioRxiv - Neuroscience 2024Quote: ... for better binding and penetration of antibodies using 4% SDS/400mM boric acid solution and 0.2% collagenase A (Millipore-Sigma, 11088793001)21,22 ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... were identified and quantified by gas liquid chromatography (Perichrom) as their alditol acetates obtained after hydrolysis (4 N trifluoroacetic acid (TFA, Sigma), 100 °C ...
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bioRxiv - Cell Biology 2024Quote: ... YFP-Rab2BS21N or YFP-Rab2BQ66L in PCF cells was induced by 10 µg/ml cumate (4-Isopropylbenzoic acid, Sigma, 268402) for 24-48 h ...
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bioRxiv - Cell Biology 2024Quote: ... 1M NaCl, 10 mM EDTA, 50 mM L-Ascorbic acid, 0.2 M disodium tartrate, 82 mM 4-nitrolphenylphosphate, all Sigma-Aldrich) added prior to incubation in the dark for 30 minutes at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: ... we added 200 μL of a freshly made 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma-Aldrich) solution 2% w/v (corresponds to 10 mg EDC in 500 μL MES buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The [5′-32P] cytidine 3′,5′-bisphosphate was prepared by incubating 1 mM cytidine 3′-monophosphate (Sigma) with 312.5 pmole [γ-32P] ATP (6000 Ci/mmol ...
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bioRxiv - Bioengineering 2020Quote: ... pH 5.8 and reacted with 600 molar excess of 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma) and 200 molar excess racemic aminomethylnicotine for 20h ...
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bioRxiv - Neuroscience 2023Quote: ... The eluates 1-3 were mixed and concentrated with Amicon Ultra-15 (Merck Millipore, MWCO 3 K) to protein concentrations of 44–162 µM.
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bioRxiv - Molecular Biology 2021Quote: ... 4-OHT (Sigma-Aldrich: H7904, 1 μg/mL) was added for the last 6 hours of doxycycline treatment to induce AsiSI translocation ...