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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Recombinant proteins used for biochemical assays were expressed from pMal-RT plasmids (see above and Table S3. For each protein preparation, E. coli Rossetta2 CapR cells (Novagen) containing freshly transformed expression constructs were plated on LB agar containing ampicillin (100 µg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... for 5 min and hybridized with smFISH probes diluted 1:3000 in hybridization buffer (10% dextran sulfate, Sigma D8906, 20% formamide, 1 mg/ml E. coli tRNA, Sigma R1753 ...
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bioRxiv - Immunology 2023Quote: ... were plated in P96 plates and infected overnight with BCG at an MOI of 10 or Mtb at an MOI of 1 or stimulated overnight with 100 ng LPS (from E. Coli 011: B4, Sigma) and 10 µM nigericin sodium salt (Sigma ...
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bioRxiv - Immunology 2023Quote: ... mice were injected intraperitoneally (i.p.) or intravenously (i.v.) with a single dose of LPS (E. coli O111:B4; Sigma Cat#L2630) 2.5 mg/kg ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated overnight at 4°C with the adequate primary antibodies in TBST buffer: mouse anti-His-tag (1:2000, Millipore, 05-949), rabbit anti-CAP-D2 serum (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... were inserted between the NdeI and XhoI sites of the bacterial expression vector pET-24a containing a HIS-tag (Novagen, Madison, USA). The ligated products were transformed into TOP10 E ...
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bioRxiv - Microbiology 2021Quote: ... from ffmA was PCR amplified and cloned in frame as a NdeI/HindIII fragment upstream of the C-terminal 6x-His tag in pET29a+ (EMD Millipore, Inc.) to form plasmid pSP113 ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of WsGT4 and WsGT6 was cloned into the NheI/HindIII and NdeI/BamHI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::WsGT4 and pET28a::WsGT6 respectively (Figure S1 A&B) ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of CfADH1 and CfAKR2b was cloned into the NdeI/EcoRI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::CfADH1 and pET28a::CfAKR2b ...
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Microbiology 2020Quote: ... Anti-Measles Nucleoprotein mouse monoclonal antibody produced from hybridoma NP.cl25 (Millipore Sigma, Cat # 95051114).
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bioRxiv - Biochemistry 2020Quote: Escherichia coli strain BL21(DE3) Rosetta-2 pLysS (Novagen Merck, Darmstadt Germany) was transformed with pET28a-His6-CrTRXz and grown in 1 L of 2YT medium supplemented with kanamycin (50 µg.mL-1 ...
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bioRxiv - Microbiology 2022Quote: ... Nanobodies were expressed in Escherichia coli Rosetta-gami 2 (DE3) cells (Novagen) in autoinduction media and purified through consecutive nickel affinity chromatography and size-exclusion chromatography (see Supplementary Methods).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... (E)-2-decenal were all obtained from Sigma-Aldrich (St. Louis, Missouri, USA). Dopamine was obtained from Tocris Bioscience (Bristol ...
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bioRxiv - Microbiology 2019Quote: ... Macrophages were counted by haemocytometer and diluted to a concentration of 1×105 cells per ml in DMEM supplemented with 1 μg/ml lipopolysaccharide (LPS from E. coli, Sigma L2630) before being plated into 24 well microplates (Greiner ...
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bioRxiv - Molecular Biology 2022Quote: ... by negative selection and activated for 72 hr in the presence of LPS (50 μg/ml final concentration, E. coli 0111:B4; Sigma-Aldrich) plus IL4 (2.5 ng/ml final concentration ...
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bioRxiv - Neuroscience 2021Quote: ... MDMi cells were treated with 500 nM stable human Aβ1-42 oligomers (180222EMH, gift from Crossbeta) or 100 ng/mL lipopolysaccharide in PBS (LPS, L4391-1MG, E. coli 0111:B4, Sigma-Aldrich). As a control for the oligomer and LPS condition ...
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bioRxiv - Neuroscience 2022Quote: ... Time-mated pregnant rhesus macaques at 127 days of gestation (80% of term gestation) were treated with intra-amniotic LPS 1mg (E. coli O55:B5, Sigma-Aldrich) diluted in 1 mL of sterile saline (LPS) ...
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
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bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...
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bioRxiv - Immunology 2020Quote: ... 180 uL of co-culture media (RPMI-1640 media containing 1% FBS, LPS (100 ng/mL; from E coli, Sigma-Aldrich) and IFNɣ (100 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... in the central-left abdomen area and 10 μg of LPS (from E. coli, serotype 055:B5 and deproteinized; Sigma-Aldrich) in 100 μl of PBS in the centralright abdomen area ...
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bioRxiv - Neuroscience 2022Quote: ... media was aspirated and cells were exposed to 10/50 μg/mL Lipopolysaccharide (LPS; E. coli strain 0111:B4; Sigma-Aldrich) (MilliQ H2O vehicle ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were stimulated for 4 h with one of the following: LPS (E. coli 0127:B8 100 ng/ml; Sigma-Aldrich); solutions of mix of synthetic neuromelanins (NM 10 μg/mL of Pheo-β-LG and 10 μg/mL of Pheo-β-fl) ...
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bioRxiv - Neuroscience 2022Quote: ... media was aspirated and cells were exposed to 10 or 50 μg/mL lipopolysaccharide (LPS) (E. coli strain 0111:B4; Sigma-Aldrich) (MilliQ H2O vehicle ...
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bioRxiv - Systems Biology 2023Quote: ... All E. coli strains (a gift from Dr. Christian Rudolph) were routinely grown in modified Luria Broth (LB) (1% tryptone (Sigma-Aldrich), 0.5% yeast extract (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... either fresh or thawed DCs (differentiation day 8) were stimulated for 24 h with 200 ng/ml lipopolysaccharide (LPS; E. coli O26:B6, Sigma-Aldrich) and used for experiments on day 9 ...
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bioRxiv - Immunology 2023Quote: ... CD11c+ were purified and either plated with no further supplementation or stimulated with 100 ng/mL or 1 μg/mL of lipopolysaccharide (LPS, E. coli O111:B4, Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... 8-week old male and female C57BL/6J mice (n=2/sex/condition) were intraperitoneally injected once with 1.0 mg/kg lipopolysaccharide (LPS; Lipopolysaccharides from E. coli O55:B5, Sigma-Aldrich L5418) or vehicle solution 1xPBS (Phosphate buffered saline ...
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bioRxiv - Neuroscience 2023Quote: ... 8-week old male and female Cx3cr1-GFP mice bred on a C57BL/6J background (n=2 females and 1 male/condition) were intraperitoneally injected with 0.5 mg/kg lipopolysaccharide (LPS; Lipopolysaccharides from E. coli O55:B5, Sigma-Aldrich L5418) or vehicle solution (PBS ...
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bioRxiv - Genetics 2023Quote: ... each probe set was added (final concentration of 500 nM) to 20mM VRC and hybridization buffer (1% Dextran sulfate [EMD Millipore], 1 mg/mL E. coli tRNA [Sigma] ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Systems Biology 2021Quote: ... The N-terminal His-tag was digested at room temperature overnight using up to 12 NIH Units per mL of bovine thrombin (Sigma-Aldrich T4648-10KU). Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... in the same buffer containing 1/5000 of primary antibody raised against poly-His tag directly coupled with peroxidase (Sigma, catalog no. A7058). After washing 3x by PBS 1x ...
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bioRxiv - Molecular Biology 2019Quote: ... His-GFP or His-fusion proteins were bound to HIS-Selec HF Nickel Affinity Gel (Sigma) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... strep tag and myc tag were obtained from Sigma, IBA and Abcam ...
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bioRxiv - Immunology 2021Quote: ... Infected cells were detected using influenza A nucleoprotein-specific monoclonal antibodies (Millipore, Burlington, MA, USA). MN titers were reported as the reciprocal of the highest serum dilution with ≥50% neutralization.
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Neuroscience 2021Quote: ... treated with poly-d-lysine(Sigma-Aldrich A-003-E, 1-2 hr at 37°C, 2 μL/well) followed by laminin (Thermo Fisher 23017015 ...
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bioRxiv - Bioengineering 2019Quote: ... coli in 2% alginate solution supplemented with 2% Luria-Bertani (LB) broth (Sigma Aldrich, St. Louis, MO, USA). For ink preparation ...
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bioRxiv - Synthetic Biology 2021Quote: ... rhaeticus cultures were grown shaking to mid exponential phase. Cells treated with 50 nM (E. coli) or 50 μM (K. rhaeticus) of AHL (N-Acyl homoserine lactone, Sigma-Aldridge K3255) and induced for approximately 4 doubling times (i.e ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli cell-free TXTL extract was prepared from BL21 Rosetta 2 from Novagen, as described previously.23,24 The TXTL system is commercially available as the product “myTXTL” from Arbor Biosciences ...
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bioRxiv - Immunology 2020Quote: ... coli lipopolysaccharides (LPS, 0.08, 0.4, and 2 μg/ml; L2630-10MG, Sigma, USA), S ...
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bioRxiv - Plant Biology 2022Quote: ... coli strains derived from BL21(DE3) and Rosetta 2 (Merck Millipore, Burlington, USA), BL21(DE3)-R3-pRARE2 and BL21(DE3)-R3-lambda-PPase ...
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bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
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bioRxiv - Cancer Biology 2021Quote: ... was prepared in 2% vitamin E/d-a-tocopheryl polyethylene glycol 1000 succinate (Sigma) immediately prior to IV administration by tail vein injection at 25 mg/kg ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were stained with H&E and 2% Alizarin Red solution (all Sigma-Aldrich).
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bioRxiv - Cell Biology 2019Quote: ... α-His (Sigma H1029). α-Rabbit IgG HRP conjugate (Promega W4011) ...
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bioRxiv - Biochemistry 2020Quote: ... PCNA-12 co-expression plasmids were constructed by inserting non-tagged PCNA-1 into multiple cloning site 1 and PCNA-2 (without and with a His6-tag) into site 2 of pET-DUET-1 plasmid (Novagen).