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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for Recombinant Mouse Havcr1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... FCS was obtained from Sigma-Aldrich. HEK293 and HEK293T cells (gifts from Caetano Reis e Sousa ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10 % FCS (Sigma-Aldrich), 100 U/mL Penicillin and 100 μg/mL Streptomycin (Gibco ...
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bioRxiv - Synthetic Biology 2022Quote: ... supplemented with 15% FCS (Sigma, C8056), 100 U/ml penicillin-streptomycin (Cellgro ...
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bioRxiv - Neuroscience 2022Quote: ... 10% FCS (Sigma-Aldrich, Germany, EU), 2 mM L-Glutamine (Biochrom ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% FCS (Sigma-Aldrich) and 1% PenStrep (GIBCO) ...
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bioRxiv - Molecular Biology 2020Quote: ... in FC-40 (Sigma Aldrich, USA). The devices were incubated at 95 °C overnight and afterward stored at room temperature until further usage for droplet production.
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bioRxiv - Genomics 2021Quote: ... with 10% FCS (Sigma, Cat # F0926), with 100U/mL Pen-Strep (Gibco ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% FCS (Sigma Aldrich), L-glutamine (2◻mM ...
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bioRxiv - Immunology 2021Quote: ... 10% fetal calf serum (FCS) (Sigma), 0.1 U/ml penicillin (Lonza) ...
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bioRxiv - Neuroscience 2022Quote: ... 10% FCS (Sigma-Aldrich, Germany, EU), 2 mM L-Glutamine (Biochrom ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% FCS (Sigma-Aldrich), 2 mM GlutaMAX (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% FCS (Sigma-Aldrich), 2 mM GlutaMAX (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Sigma-Aldrich), Pen-Strep (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Sigma-Aldrich), PenStrep (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... containing 10% FCS (Sigma-Aldrich, #F7524), 100 U/mL penicillin and 0.1 mg/mL streptomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Sigma-Aldrich) and PenStrep (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Sigma-Aldrich), PenStrep (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FCS (Sigma, F2442), pen-strep ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Sigma-Aldrich) and PenStrep (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Sigma-Aldrich) and PenStrep (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% FCS (Sigma, F2442), GlutaMAX 1x (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated overnight at 4°C with the adequate primary antibodies in TBST buffer: mouse anti-His-tag (1:2000, Millipore, 05-949), rabbit anti-CAP-D2 serum (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... We prepared InsP7 by using 100ng of Mouse IP6K1 (mIP6K1) recombinant protein along with 100 µM of InsP6 (Sigma, USA) in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Systems Biology 2020Quote: ... At time 0 medium was removed from cells and recombinant mouse tumor necrosis factor alpha (TNF-α, Sigma-Aldrich, #T7539) was added at concentrations 0-100 ng/mL as 5-minute pulse ...
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bioRxiv - Developmental Biology 2019Quote: ... The serum-containing mESCs medium was composed of high glucose DMEM (HyClone) supplemented with 1000 U/mL recombinant mouse leukaemia inhibitory factor (LIF) (Millipore), 100 μM non-essential amino acids (NEAA ...
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bioRxiv - Developmental Biology 2020Quote: ... The trophectoderm was removed by immunosurgery and “epiblasts” were grown in gelatinised dishes in the presence of NDiff + 2i and ESGRO (recombinant mouse LIF Protein, Millipore) to establish ESC lines.
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bioRxiv - Neuroscience 2023Quote: ... we randomly assigned Ai9:Tmem119cre mice to receive an intravenous injection of control solution or 0.05 mg/kg of recombinant IFNγ from mouse (Sigma #I4777), minutes before initiating the rupture of cortical capillaries and then 12h later ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in the presence of recombinant mouse IL-4 (25 ng/mL; #404-ML, R&D) and LPS (20 μg/mL; #L2880, Sigma) over night to induce B cell activation and terminal differentiation ...
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bioRxiv - Microbiology 2023Quote: ... 21 and 42 with 50 μg of recombinant LMW630 mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA). Enzyme-linked immunosorbent assay was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then washed with buffer and recombinant Tau protein (Sigma recombinant Tau protein #T0576) and ATP (Sigma #A1852 ...
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bioRxiv - Cell Biology 2021Quote: ... peroxidase-anti-peroxidase for detecting TAP-tagged proteins (Sigma); anti-FLAG mouse monoclonal antibody (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... and dextran tagged with fluorescein (40,000 MW, Sigma Aldrich) were resuspended in 100 μl HBSS (4 mg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged protein was immunoprecipitated using FLAG antibody (Sigma) and Protein G magnetic beads (Invitrogen) ...
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bioRxiv - Genetics 2019Quote: ... FLAG-tagged protein was immunoprecipitated using FLAG antibody (Sigma) and Protein G magnetic beads (Invitrogen) ...
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bioRxiv - Developmental Biology 2019Quote: Biotin-tagged OSTN was labeled by Cy3-streptavidin (Sigma). Equal volume of 25 μM biotin-tagged OSTN and 0.1 mg/ml Cy3-streptavidin were incubated with on ice for 15 minutes at 4 C ...
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bioRxiv - Molecular Biology 2023Quote: ... HTP-tagged proteins were detected using rabbit PAP (Sigma) diluted 10,000 fold ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with 3-isobutylmethyl-xanthine (1 mM)-containing medium or including the secondary antibody anti-mouse IgG (Fc specific, M2650, Sigma-Aldrich) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143, Sigma Aldrich, USA; 30 min at 4°C).
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bioRxiv - Immunology 2021Quote: ... Wells were washed 3 times with washing buffer then 100 μL of diluted goat anti-mouse IgG (Fc specific)-Peroxidase antibody (#A0168; Sigma-aldrich) were added ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed 4 times with PBS/Tween and then incubated for 1 h at RT with 100 μL/well of an anti-mouse Fc conjugated to alkaline phosphatase (Sigma-Aldrich), diluted 1:1000 in blocking buffer ...
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bioRxiv - Immunology 2022Quote: ... fibroblasts and MCF 10A cells were incubated with the FITC bound anti-mouse IgG secondary immunoglobulin (Fc specific) (Cat # F4143, Sigma-Aldrich) secondary antibody for 2 h at room temperature away from light ...
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bioRxiv - Biochemistry 2022Quote: ... under agitation at room temperature for 20 min and re-probed with HRP-conjugated poly-clonal goat anti-mouse IgG Fc (1:5000 dilution, Sigma–Aldrich) for checking the integrity of the mouse IgG2b Fc domain of the fusion protein ...
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bioRxiv - Molecular Biology 2022Quote: ... and expression levels of AGAL-FLAG tagged proteins were assessed by Western blot with mouse monoclonal anti-FLAG antibody (F1804, SIGMA-Aldrich, Prague, Czech Republic). Clones demonstrating similar GLA_FLAG transcript and AGAL-FLAG protein amounts were selected for further analyses.
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bioRxiv - Molecular Biology 2023Quote: ... Histidine tagged PDE4 and GST tagged CP proteins were detected chromogenically using anti-mouse secondary antibodies labelled with alkaline phosphatase (1:30000) (cat#A3562, Millipore Sigma, Burlington, MA, USA) and western blue stabilized substrate for alkaline phosphatase (Promega ...
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bioRxiv - Genetics 2023Quote: ... The presence of the tagged protein was identified by immunoblotting using anti-FLAG antibody (F1804 monoclonal antibody, clone M2 in mouse, SLCK5688, Sigma Aldrich, Supplemental Fig 2F). Rescue was confirmed by measuring cilia length ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...